YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Immunoactivities of the Protein-Polysaccharides of the Tips of the Growing Carpophores of Ganoderma lucidum

영지버섯(Ganoderma lucidum) 생장점으로부터 분리한 단백다당류의 면역활성

  • Chung, Kyeong-Soo (Laboratory of Microbial Chemistry, College of Pharmacy, Chung-Nam National University) ;
  • Kim, Sang-Bum (Laboratory of Microbial Chemistry, College of Pharmacy, Chung-Nam National University) ;
  • Chung, Soo-Hyun (Laboratory of Microbial Chemistry, College of Pharmacy, Chung-Nam National University)
  • 정경수 (충남대학교 약학대학 미생물약품화학교실) ;
  • 김상범 (충남대학교 약학대학 미생물약품화학교실) ;
  • 정수현 (충남대학교 약학대학 미생물약품화학교실)
  • Published : 1997.02.01
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Abstract

Two protein-polysaccharide fractions, GLA and GLB, respectively, were prepared from the pileus of the fully grown carpophores and the tips of the growing carpophores of Ganod erma lucidum. At a dose of 100mg/kg/day ip, GLA and GLB inhibited the growth of sarcoma 180 solid tumor in ICR mice by 56.3% and 81.8%, respectively. In a flow cytometric (FCM) analysis, GLA and GLB enhanced the formation of lymphoblasts of BALB/c, splenic leukocytes at a concentration of 100 ${\mu}$g/ml, by 38.3% and 61, 3%, respectively. When ip injected into ICR mice, GLB exerted anti-leukopenic effect against cyclophospamide (75mg/kg, ip) in that the leukocyte counts of the peripheral blood of the normal and the cyclophosphamide-treated mice. respectively. was (11.1 ${\pm}$ 3.8) ${\times}$ 10$^3$ and (4.0 ${\pm}$ 1.8) ${\times}$ 10$^3$, while the GLB-cyclophosphamide treated mice showed a leukocyte count of (10.8 ${\pm}$ 5.1) ${\times}$ 10$^3$ CELLS/${\mu}$l. These results suggest that GLB is a promising candidate for an effective, cancer immunotherapeutic agent.

Keywords

References

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