YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of EGF against Oxygen Radical-Induced Neurotoxicity in Cultured Spinal Dorsal Root Ganglion Neurons of Mouse

산소자유기에 의해 저해된 배양 척수감각 신경절 세포에 대한 상피세포성장인자의 영향

  • Park, Seung-Taeck (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Kim, Hyung-Ryong (Department of Dental Pharmacology, and School of Dentistry, Wonkwang University) ;
  • Chae, Han-Jung (College of Pharmacy, Wonkwang University)
  • 박승택 (원광대학교 의과대학 해부학교실) ;
  • 김형룡 (원광대학교 치과대학 치과약리학교실) ;
  • 채한정 (원광대학교 약학대학)
  • Published : 1997.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to elucidate the cytotoxic effect of oxygen radicals on cultured spinal dorsal root ganglion(DRG) neurons derived from mouse. the neurotoxic effect of oxygen radicals w as examined after cultured DRG neurons were exposed to xanthine oxidase(XO) and hypoxanthine(HX)-oxygen radical generating system. In addition. neuroprotective effect of epidermal growth factor(EGF) against oxidant-induced neurotoxicity was also evaluated in these cultures. The results were, as follows: 1. Lethal concentration 50(LC$_{50}$) was 35mU/ml XO and 0.1mM HX in cultured DRG neurons. 2. Oxygen radicals induced the morphological changes such as the decrease of cell number and loss of neurites in these cultures. 3. EGF increased the cell viability and neurofilament in neurons damaged by oxygen radicals. From above the results, it is suggested that oxygen radicals have a cytotoxic effect on cultured DRG neurons of neonatal mouse and selective neurotrophic factors such as EGF are, effective, in blocking the neurotoxicity induced by oxygen radicals in cultured spinal DRG neurons.

Keywords

References

  1. J Neurosci Res. v.37 Oxygen radical induced neurotoxicity in spinal cord neuron culture Michikawa M;Lim KT;McLarnon JG;Kim SU
  2. Neurology v.42 Glutamate receptors in the substentia nigra of Parkinson's disease brains Difazio MC;Hollingsworth Z;Young AB;Penny JB
  3. Nature (London) v.362 Mutation in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis Rosen D;Siddique T;Patterson D Figlewiez D;Sapp P Hentati A;Donaldson D;Toto J;O Regan J;Deng H;Rahmani Z;Krizus A
  4. Free Rad Biol Med v.12 NGF restores decrease in catalase activity and increases superoxide dismutase and glutathione peroxidase activity in the brain of aged rate Nistico G;Ciriolo MR;Fiskin D;Iannone M;DeMartino A;Rotilio G
  5. J Neurosci Res v.36 Glutamate neurotoxicity in mesencephalic dopaminergic neurons in culture Kikuchi S;Kim SU
  6. J Neurchem. v.51 Excitatory amino acid release from rat hippocampal slices as a consequence of free-radical formation Pellegrini-Giampietro DE;Cherici G;Alesiani M;Carla V;Moroni F
  7. Nature(London) v.336 NMDA receptors activate the arachidonic acid cascade system in striatal neurons Dumuis A;Sebben M;Haynes L;Pin J-P;Bockaert J
  8. Acta Physiol Suppl v.492 Cell penetration by exogenous superoxide dismutase Michelon AM;Puget K
  9. Br Med Bull v.45 Fibroblast growth factors Baird A;Walicke P
  10. Exp Neurol v.121 Growth factors prevent mitochondrial dysfunction, loss of calcium homeostasis and cell injury, but not ATP depletion in hippocampal neurons Mattson MP;Zang Y;Bose S
  11. Neuroscience v.25 Long-term culture of human fetal spinal cord neurons: Morphological immunocytochemical anelectrophysiological characteristics Kim SU;Osborne D;Kim MW;Spigelmam I
  12. Biochem Pharmacol. v.15 Metabolic studies of allopurinol, an inhibitor of xanthine oxidase Elion GB;Kovensky A;Hitchings GH;Metz E;Rundles RW
  13. Myelination and Demyelination v.219 Studies of myelin breakdown in vitro Johnson D;Toms R;Weiner H;Kim SU(ed)
  14. J Neurosci Res v.29 Oligodendrogial cell death induced by oxygen radicals and its protection by catalase Kim YS;Kim SU
  15. Stroke v.14 A possible role of lipid peroxidation in cellular damages caused by cerebral ischemia and protective effect of alpa-to-copherol administration Yamamoto M;Shima T;Uozmi T;Sogabe T;Yamada K;Kawasaki T
  16. Biochem Biophys Res Commun v.177 Purification of recombinant midkine and examination its biological activities: functional comparison of new heparin binding factors Muramatsu H;Muramatsu T
  17. J Biol Chem v.247 Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization Savage CR Jr;Cohen S