한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
권호 표지

RAPD(Random Amplified Polymorphic DNA) 검정을 이용한 한국 표고균주의 계통분류

Classification of Korean Lentinula edodos Strains by Random Amplified Polymorphic DNA (RAPD) Markers

  • 이태수 (임업연구원 산림미생물과) ;
  • 박원철 (임업연구원 산림미생물과) ;
  • 강호덕 (임업연구원 산림미생물과) ;
  • 김세권 (임업연구원 산림미생물과) ;
  • 변병호 (임업연구원 산림미생물과) ;
  • 이창근 (임업연구원 산림미생물과) ;
  • 이원규 (임업연구원 산림미생물과) ;
  • 민두식 (충북대학교 농과대학)
  • Lee, Tai-Soo (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Bak, Won-Chull (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Kang, Ho-Duck (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Kim, Se-Kwon (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Byun, Byung-Ho (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Yi, Chang-Keun (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Lee, Won-Kyu (Department of Forestry Microbiology, Forestry Research Institute) ;
  • Min, Du-Sik (College of Agriculture, Chungbuk National University)
  • 발행 : 1997.09.30
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초록

한국의 7가지 대표적인 표고품종에 대하여 RAPD(Random Amplified Polymorpic DNA) 검정을 실시하여 품종간의 구분이 가능한 지를 시도하였다. 표고 품종간의 계통분류에 적합한 RAPD marker를 생성시키기 위하여 OPA-01에서 OPA-20까지 20개의 primer를 사용한 결과, 9가지의 primer는 품종식별에 유용한 RAPD pattern을 보였으나, 나머지 11가지의 primer는 품종 식별에 사용하기 어려운 것으로 나타났다. 9가지 primer중 7개 품종을 모두 구분할 수 있는 단일 primer는 없었지만, 9가지중 2개의 조합을 취하면 어떤 경우도 7개의 표고 품종을 구분지울 수 있음으로써 RAPD 검정법이 표고 계통의 분류에 매우 정밀한 방법임을 알 수 있다.

Random Amplified Polymorphic DNA (RAPD) assay was used to identify seven typical Lentinula edodes (Berk.) Pegler strains isolated in Korea. Twenty primers from OPA-01 to OPA-20 were applied to generate the recognition of L. edodes strains. Out of 20 primers, nine primers showed efficient RAPD patterns to classify the 7 strains tested, but the rest eleven primers were not useful to be used. Even though there was no single primer that could classify all of the strains, any combination of two primers among the nine primers could identify the strains tested. Thus, RAPD assay turned out to be very precise method for classifying L. edodes strains.

키워드

참고문헌

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