송이균사(Tricholoma matsutake DGUM 26001)의 생육에 미치는 탄소원 및 비타민의 영향

Effect of Carbon sources and Vitamins on Mycelial Growth of Tricholoma matsutake DGUM 26001

  • 발행 : 1997.09.30

초록

경주시 남산으로부터 송이를 채집하여 균사를 분리하였다. 집락 및 균사의 형태, 균사의 독특한 송이향(1-octene-3-ol)을 기준으로 하여 Tricholoma matsutake DGUM 26001이라 명명하였다. T. matsutake DGUM 26001은 사용한 복합액체배지 중 yeast malt extract 및 Czapek-Dox 배지에서 가장 우수한 균사생육을 보여주었으며, nutrient glucose 배지, Macaya-Lizano 배지, 및 Tricholoma matsutake 배지(TMM)에서의 균사생육은 양호하였다. 세균 배양에 사용되는 colombia 배지, brain heart infusion 배지, Luria-Bertani glucose 배지 및 brucella 배지에서의 균사 생육은 매우 저조하였다. TMM 배지에 다양한 탄수화물을 탄소원으로 첨가하였을 때, 다당류의 starch, 이당류의 trehalose 및 maltose, 단당류의 sorbitol, xylitol 및 glucose를 사용하였을 때 균사생육이 양호하였다. Starch를 탄소원으로 하는 TMM 배지에서 30일간 균사를 배양하였을 경우, 8.85 g/L의 건조 균사중량을 얻을 수 있었다. 포도당을 탄소원으로 하는 TMM 액체배지에 coconut water 및 pyridoxine을 비타민으로 첨가하여 30일간 배양 후, 가장 우수한 균사 건조체를 얻을 수 있었으며 각각 8.65 및 8.32 g/L의 균사 건조량을 얻을 수 있었다.

The mycelium was isolated from the fruiting body of Tricholoma matsutake collected from Mt. Namsan, Kyongju and it was named as Tricholoma matsutake DGUM 26001. For the mycelial growth of T. matsutake DGUM 26001, the complex media, yeast-malt extract medium and Czapek-Dox medium supplemented with yeast extract, were excellent. The media such as nutrient glucose medium, mushroom complex medium, and Tricholoma matsutake medium (TMM), were effective. However, There was no a mycelial growth in the media used for bacterial cultivation such as colombia medium, brain heart infusion medium, Luria-Bertani medium supplemented with glucose, and brucella medium. When carbohydrate as a carbon and energy source was supplemented in the TMM medium for the mycelial growth, starch as a polysaccharide was best. As a disaccharide, trehalose and maltose were excellent. Sorbitol, xylitol and glucose were excellent carbon sources of monosaccharose. When the mycelia were cultivated for 30 days at $24^{\circ}C$ in the TMM supplemented with 2.0% starch, the dry weight of the mycelia harvested was 8.85 g/L. When organic acid was given as a carbon source, only succinic acid was utilized. As a vitamin source, coconut water and pyridoxine were excellent. After 30 day-cultivation in the TMM medium, the dry weights with coconut water and pyridoxine were 8.65 and 8.32 g/L, respectively.

키워드

참고문헌

  1. J. Kor. Soc. Food Nutr. v.15 no.3 Studies on the volatile aroma components of edible mushroom (Tricholoma matsutake) of Korea Ahn, J.S.;Lee, K.H.
  2. Kor. J. Food Sci. Technol. v.16 no.2 Storage of pine agaric by irradiation combined with natural low temperature Cho, H.O.;Byun, M.W.;Kwon, J.H.
  3. J. Microbiol. v.33 no.2 Nucleotide sequence analysis of the 5S ribosomal RNA gene of the mushroom Tricholoma matsutake Hwang, S.K.;Kim, J.G.
  4. Kor. J. Mycol. v.9 no.4 The present status of matsutake production technique in Japan Ito, T.
  5. Trans. Mycol. Soc., Japan v.28 Behavior of cell nuclei during basidiospore formation in Tricholoma matsutake Iwase, K,;Matsui, S.;Taniguchi, T.;Obayashi, A.
  6. Distribution of the ectomycorrhizal fungus Tricholoma matsutake and the related species and some characteristics of their isolates Iwase, K.
  7. Trans. Mycol. Soc., Japan v.29 Acceleration of fruit-body formation on the environmental control in an area with shiro of Tricholoma matsutake Iwase, K.;Ito, T.;Fujita, H.;Matsui, S.;Taniguchi, T.;Obayashi, A.
  8. Kor. J. Mycol. v.17 no.2 Studies on analyzing meteorological elements related with yield of Tricholoma matsutake (S. Ito et Imai) Singer Kang, A.S.;Cha, D.Y.;Kim, Y.S.;Park, Y.H.;You, C.H.
  9. Trans. Mycol. Soc., Japan v.17 Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. IV. Studies on a seed culture and a trial for the cultivation on solid media Kawai, M.;Ogawa, M.
  10. Trans. Mycol. Soc., Japan v.18 Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. V. Effects of some chemicals on the growth of mycorrhizal and non-mycorrhizal soil fungi Kawai, M.;Ogawa, M.
  11. Kor. J. Mycol. v.19 Biology of Tricholoma matsutake found at Pinus densiflora communities in the areas of Kyoung Sang Do Lee, S.S.
  12. Kor. J. Mycol. v.25 no.2 The mycelia isolated from the basidiocarps of Tricholoma matsutake in Korea Lee, S.S.;Sung, C.K.
  13. For. Rep. For. Res. Inst. Korea v.31 Studies on the artificial cultivation and propagation of pine mushroom (II)-Increasing yield and promoting quality of pine mushroom by the cap-covering or soil-covering- Lee, T.S.;Park, C.J.;Shim, W.S.;Kim, S.H.;Ju, Y.W.;Oh, S.W.;Jo, J.M.
  14. Trans. Mycol. Soc., Japan v.17 Microbial ecology of 'Shiro' in Tricholoma matsutake (S. Ito et Imai) Sing. and its allied species. II. Tricholoma matsutake in Pinus pumila var. yezoalpina forest Ogawa, M.
  15. Trans. Mycol. Soc., Japan v.17 Microbial ecology of 'Shiro' in Tricholoma matsutake (S. Ito et Imai) Sing. and its allied species. III. Tricholoma matsutake in Picea glehnii and Picea glehnii-Abies sachalinensis forests Ogawa, M.
  16. Trans. Mycol. Soc., Japan v.18 Microbial ecology of 'Shiro' in Tricholoma matsutake (S. Ito et Imai) Sing. and its allied species. IV. Tricholoma matsutake in Tsuga diversifolia forests Ogawa, M.
  17. Kor. J. Mycol. v.9 no.4 Mycorrhiza in the pine forest the ecological study of matsutake as a microorganism Ogawa, M.
  18. Trans. Mycol. Soc., Japan v.16 Primordia formation of Tricholoma matsutake (S. Ito et Imai) Sing. in pure culture Ogawa, M.;Hamada, M.
  19. Trans. Mycol. Soc., Japan v.17 Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. III. Effects of growth promoting natural products on the vegetative growth of T. matsutake Ogawa, M.;Kawai, M.
  20. Trans. Mycol. Soc., Japan v.19 On the primary stage in 'Shiro' formation of Tricholoma matsutake Ogawa, M.;Ito, T.;Kobayashi, T.F.;Fujita, H.
  21. Trans. Mycol. Soc., Japan v.19 Microbial ecology of 'Shiro' in Tricholoma matsutake (S. Ito et Imai) Sing. and its allied species. VIII. Tricholoma bakamatsutake Hongo in Quercus mongoloca var. grosserrata forest and Q. serrata forests Ogawa, M.;Ohara, H.
  22. Japan For. Soc. v.60 Cultivation method of the mycorrhizal fungus, Tricholoma matsutake (S. Ito et Imai) Sing. (I) Growing method of the pine saplings infected with T. matsutake in the field Ogawa, M.;Takeo, U.;Shuji, K.;Kisoo, Y.
  23. Trans. Mycol. Soc., Japan v.24 Quantitative analysis of odorous compounds in the fruitbodies of Tricholoma matsutake Ohta, A.
  24. Trans. Mycol. Soc., Japan v.27 Basidiospore germination of Tricholoma matsutake (I). Effects of organic acids on swelling and germination of the basidiospores Ohta, A.
  25. Trans. Mycol. Soc., Japan v.27 Basidiospore germination of Tricholoma matsutake (II). Evaluations of germination conditions and microscopic observations of germination stages Ohta, A.
  26. Trans. Mycol. Soc., Japan v.29 Effects of butyric acid and related compounds on basidiospore germination of some mycorrhizal fungi Ohta, A.
  27. Trans. Mycol. Soc., Japan v.31 A new medium for mycelial growth of mycorrhizal fungi Ohta, A.
  28. Trans. Mycol. Soc., Japan v.19 Fruit-bodies of Tricholoma matsutake appeared on a pot of dwarfed pines Okazawa, K.
  29. Kor. J. Mycol. v.8 no.1 A study on multiplication of Tricholoma matsutake Ryoo, C.I.;Nam, S.U.;Lee, J.Y.;Lee, S.K.
  30. Trans. Mycol. Soc., Japan v.20 Comparative studies on morphological characteristics of the colonies of Tricholoma matsutake, T. fulvocastaneum, and T. bacamatsutake on agar media Shimazono, H.
  31. Kor. J. Mycol. v.19 no.2 Microfungal flora of Tricholoma matsutake producing and nonproducing sites in the forest of Pinus densiflora Song, H.S.;Min, K.H.
  32. Trans. Mycol. Soc., Japan v.28 Purification and some properties of carboxyl proteinases from Tricholoma matsutake Terashita, T.;Kono, M.
  33. Mem. Fac. Agr. Kinki Univ. v.22 Carboxyl proteinases from Tricholoma matsutake and its related species Terashita, T.;Kono, M.
  34. Trans. Mycol. Soc., Japan v.20 Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. VII. Antibiotic activities of volatile substances extracted from a 'Shiro' of T. matsutake Tsuruta, T.;Kawai, M.
  35. Trans. Mycol. Soc., Japan v.28 In vitro cultures of Tricholoma matsutake and Pinus densiflora Yokoyama, R.;Yamada, T.