한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
권호 표지

목재부후균인 Phanerochaete chrysosporium의 원형질체 생성 및 재생

Protoplast Formation and Regeneration of the Wood-Rot Basidiomycete Phanerochaete chrysosporium

  • 발행 : 1995.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Phanerochaete chrysosporium ME446의 원형질체 생성 및 재생을 위한 원형질체 분리에 알맞은 세포벽 분해효소로는 Novozym 234이였으며 삼투압 조절제로서는 0.6M sucrose, 처리시간은 $2.5{\sim}3$시간이었다. 균사체 배양일수는 대수기에 해당하는 42시간이었으며 원형질체 재생에 알맞은 배지로는 0.6M mannitol을 첨가한 poly-R 배지였다.

To investigate optimal conditions for the protoplast formation and regeneration of Phanerochaete chrysosporium, preparations of three enzymes were used to liberate protoplasts from its 20 hrs-old mycelium on cellophan membrane covered agar media. Novozym 234 alone with 0.6M sucrose was the most effective for isolation of protoplasts from the mycelium with 3hrs incubation time at $39^{\circ}C$ in shaking condition of 120 rpm. The poly-R medium stabilized with 0.6M mannitol was the best for regeneration of the protoplasts.

키워드

참고문헌

  1. Kor. J. Mycol. v.18 no.3 Studies on protoplast formation and regeneration of Coriolus versicolor Bok, J.W.;Park, S.H.;Choi, E.C.;Kim, B.K.;Yoo, Y.B.
  2. Kor. J. Mycol. v.22 no.2 Studies on protoplast formation and regeneration of Lyophyllum decastes Bok, J.W.;Kim, J.P.;Jin, M.R.;Choi, E.C.;Kim, B.K.
  3. Kor. J. Mycol. v.12 no.1 Some factors affecting the protoplast release from Pleurotus ostreatus Byun, M.O.;Go, S.J.;Park, Y.H.;Shin, G.C.
  4. Microbial and Enzymatic Degradation of Wood and Wood Components Eriksson, K.E.;Blanchette, R.A.;Ander, P.
  5. Appl. Environ. Microbiol. v.46 no.1 Formation, fusion and regeneration of protoplasts from wild=type and auxotrophic strains of the white rot basidiomycete Phanerochaete chrysosporium Gold, M.H.;Cheng, T.M.;Alic, M.
  6. Kor. J. Mycol. v.22 no.2 Isolation of protoplasts from Rhizopus nigricans Kim, M.H.;Kim, M.N.
  7. Kor. J. Mycol. v.16 no.2 Interspecific protoplast fusion of Ganolerma applanatum and Ganoderma lucidum and fruit body formation of the fusants Park, Y.D.;Yoo, Y.B.;Shin, P.G.;You, C.H.;Cha, D.Y.;Park, Y.H.;Lee, J.S.
  8. Ann. Rev. Microbiol. v.33 Fungal protoplasts: Isolation, reverastion, and fusion Peberdy, J.F.
  9. Fungal Protoplast: Applications in Biochemistry and Genetics Peberdy, J.F.;Ferenczy, L.
  10. Mehtods in Enzymol. v.161 Lignin peroxidase of Phanerochaete chrysosporium Tien, M.;Kirk, T.K.
  11. Kor. J. Mycol. v.16 no.1 Protoplast isolation and reversion from Ganoderma lucidum and Ganoderma sp. Um, S.D.;Chae, Y.A.;Yoo, Y.B.;You, C.H.;Cha, D.Y.
  12. Kor. J. Mycol. v.21 no.1 Protoplast formation and regeneration from mycelia of Phytophthora capsici Yi, S.Y.;Kim, Y.J.;Hwang, B.K.
  13. Kor. J. Mycol. v.13 no.1 Studies on protoplast isolation from edible fungi Yoo, Y.B.;Peberdy, J.F.;You, C.H.
  14. Kor. J. Mycol. v.15 no.1 Protoplast isolation and reversion from Lyophyllum ulmarium Yoo, Y.B.;You, C.H.;Park, Y.H.;Chang, K.Y.
  15. Kor. J. Mycol. v.15 no.1 Protoplast isolation and reversion from Pleurotus spodoleucus Yoo, Y.B.;Byun, M.O.;Go, S.J.;You, C.H.;Chang, K.Y.