한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제39권5호
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- Pages.465-472
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- 1994
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
저온에서 볍씨 발아에 미치는 삼투처리 효과
Effect of Osmopriming on Rice Seed Germination in Low Temperature
초록
저온에서 볍씨종자의 발아증진을 도모하기 위하여 파종 전 종자를 PEG용액에 삼투처리 하였다. 발아율을 높이기 위한 가장 적절한 PEG-6000의 농도는 20%였으며, 침지하는 동안 온도는 20
To improve the germination performance of rice seeds under suboptimal temperature, osmopriming with PEG-6000 was examined. Optimal PEG-6000 concentration to improve germination was 20% PEG-6000 solution, and rice cultivars used in this experiment were Sinunbongbyeo, Gancheokbyeo, Dongjinbyeo. The water content of seeds after soaking for 60 hours in the PEG solution is similar to that of seeds after soaking for 24 hours in the distilled water. Germination performance of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control, especially under suboptimal temperatures. Electrical conductivity of the soaked seeds in the PEG solution was lower than that of the soaked seeds in the distilled water or the control, and total dehydrogenase activity of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control. SDS-PAGE results of soluble protein from the embryos of seeds primed differently showed darker band in the seeds soaked in the PEG solution than the seeds soaked in the distilled water or the control at the 68 KD region. Also, band patterns of peroxidase and esterase of embryos soaked in the PEG solution were darked than that of embryos soaked in the distilled water or the control at the Rf 0.94 and Rf 0.87, respectively.