Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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PULMONARY XENOBIOTIC CONJUGATION IN THE ISOLATED PURFUSED RABBIT LUNG AND IN VITRO: EFFECT OF ETHANOL

  • Yang, C.Mierha (College of Phamacy Dan kuk University) ;
  • Carlson, Gary P. (College of Phamacy Dan kuk University)
  • Published : 1991.12.01
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Abstract

Pulmonary conjugation pathways may be important for the metabolism of xenobiotics introduced via airways of systemically. The objective of this study was to determine the pulmonary conjugating capacity in both the isolated perfused rabbit lung (IPRL) and in vitro, and the ability of ethanol to alter the above. The IPRL was capable of conjugating glutathione (GSH) with either 1-chloro-2,4-dinitrobenzene (CDNB) of 1,2-epoxy-(p-nitrophenoxy) propane(ENP). The pulmonary GSH conjugation with ENP was inhibited by cibacron blue, indicating the presence of glutathione-S-transferase (GST) u and/or classes, but it was not altered by buthionine sulfoximine, a selective inhibitor of Gamma-glutamylcysteine synthetase.

Keywords

References

  1. Toxicology v.36 The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats Aykac,G.;Uysal,M.;Yalcin,A.S.;Kocak-Toker,N.;Sivas,A.;Oz,H.
  2. Eur. J. Biochem. v.46 UDP-glucuronyltransferase in perfused rat liver and in microsomes: Influences of phenobarbital and 3-methylcholanthrene Bock,K.W.;White,N.H.
  3. Chem. Biol. Interact. v.42 Transient decrease of liver cytosolic glutathione S-transferase activity in rat given 1,2-bromoethane or $CCl_4$ Botti,B.;Moslen,M.T.;Triff,N.M.;Reynolds,E.S.
  4. Biochem. Pharmacol. v.32 Induction of louse glutathione-S-transferase by ethanol David,R.M.;Nerland,D.E.
  5. Bioemetrics v.11 Multiple range multiple F tests Duncan,D.B.
  6. Glucuronidation of drugs and other compounds Dutton,G.J.
  7. Life Sci. v.43 Circadian periodicity of tissue glutathione and its relationship with lipid peroxidation in rats Farooqui,M.Y.H.;Ahmed,A.E.
  8. Experimentica v.39 Time course study of the changes in blood glutathione induced by acute ethanol intoxication in the rat Fernandez,V.;Fernandez,N.;Velenzuela,A.;Videla,L.A.
  9. Methods in Enzymol. v.77 Depletion of glutathione by inhibition of biosynthesis Griffith,D.W.
  10. Proc. Natl. Acad. Sci. (U.S.A.) v.76 Glutathione: Interorgan translocation, turnover and metabolism Griffith,O.W.;Miester,M.
  11. J. Biol. Chem. v.249 Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation Habig,W.H.;Pabst,M.J.;Jakoby,W.B.
  12. Glutathione conjugation Possible mechanisms involved in overexpression of glutathione transferase, glutathione and other glutathione-dependent enzymes Hayes,J.D.;Wolf,C.R.;Sies,H.(ed.);Ketterer,B.(ed.)
  13. Drug Metab. Dispos. v.10 Influence of ethanol on glutathione content and on the activity of glutathione-S-transferase and epoxide hydrase in the rat Hetu,C.;Yelle,L.;Joly,J.L.
  14. Toxicology v.16 Hepatic drug metabolism during ethanol ingestion in riboflavin deficient rats Hietanan,E.;Koivusaari,U.;Laittilatinen,M.;Norling,A.
  15. The Toxicologist v.9 Immunoprecipitation of ethanol-induced rabbit UDP-glucuronyltransferase (GT) by p-nitrophenol GT antibodies Hutabarat,R.M.;Green,M.D.;Tephly,T.R.;Yost,G.S.
  16. Arch. Biochem. Biophys. v.273 Purification and characterization of an ethanol-induced UDP-glucuronyltransferase Hutabarat,R.M.;Yost,G.S.
  17. Anal. Biochem. v.86 Measurement of cytochrome P-450 in the presence of large amount of contaminating hemoglobin and methemoglobin Johannesen,K.A.M.;DePierre
  18. Hepatology v.10 Lipid peroxidation and antioxidant defence system in rat liver after chronic ethanol feeding Kawase,T.;Kato,S.;Lieber,C.S.
  19. Pharmacol. Res. Commun. v.17 Influence of acute ethanol administration on hepatic glutathione peroxidase and glutathione transferase activities in the rat Kocak-Toker,N.;Uysal,M.;Aykac,G.;Sivas,A.;Yalcin,S.;Oz,H.
  20. J. Pharmacol. Exp. Ther. v.35 Glucuronidation in isolated perfused rat intestine segments after mucosal and serosal administration of 1-naphthol Koster,A.S.;Noordhook,J.
  21. Hepatology v.10 Experimental methods of ethanol administration Lieber,C.S.;DeCarli,M.;Notley,K.A.
  22. J. Biol. Chem. v.193 Protein measurement with Folin reagent Lowey,O.H.;Posebrough,N.J.;Farr,A.L.;Randall,R.J.
  23. Xenobiotica v.8 The influence of dose on the pattern of conjugation of phenol and 1-naphthol in non-human primites Metha,R.;Hirom,P.C.;Millburn,P.
  24. Kinetics and Mechanism Moor,J.W.;Pearson,R.G.
  25. Biochem. J. v.125 The heterogeneity of uridine-diphosphate glucuronyltransferase from rat liver Mulder,B.J.
  26. J. Biol. Chem. v.239 The carbon-monoxide binding pigmant of liver microsomes. I. Evidence of its hemoprotein nature Omura,T.;Sato.R.
  27. Biochem. Pharmacol. v.35 Diminished rates of glucuronidation an sulfation in perfused rat liver after chronic ethanol administration Reinke,L.A.;Moyer,M.J.;Notley,K.A.
  28. Carcinogenesis v.7 Glutathione transferase in rat lung: The presence of transferase 7-7, highly efficient in the conjugation with carcinogenic, (+)-7β, 8α-dihydroxy-9α, 10α-oxy-7,8,9,10-tetrabenzo[a]pyrene Robertson,I.G.C.;Jensson,H.;Mannervik,G.;Jerstron,B.
  29. Arch. Biochem. Biophys. v.15 Purification and biochemical characterization of the rabbit pulmonary glutathione-S-transferase: stereoselectivity and activity toward pyrene 4,5-oxide Seragjit-Singh,C.J.;Bend,J.R.
  30. Biochem. J. v.226 Hepatic thiol and glutathione efflux under the influence of vasopressin, phenylephrine and adrenaline Sies,H.;Graf,P.
  31. Biochem. J. v.243 Glutathione-S-transferase of mouse lung: Selective binding of benzo[a]pyrene metabolites by the subunits which are preferentially induced by t-buthylated hydroxyanisole Singh,S.V.;Creeadon,G.;Das,M.;Hukhtar,H.;Awasthi,Y.C.
  32. Alcoholism (NY) v.12 Depeletion of hepatic glutathione by ethanol occurs independently of ethanol metabolism Spiesky,H.;Kera,Y.;Penttila,K.E.;Isrel,Y.;Lindros,K.O.
  33. Biochem. Pharmacol. v.34 Gamma-glutamylcysteine: a substrate for glutathione-S-transferases Sugimoto,M.;Kuhlenkamp,J.;Ookhtens,M.;Aw,T.W.;Reeve,Jr.J.;Kaplowits,N.
  34. Biochem. Pharmacol. v.36 Effect of ethanol feeding on hepatic microsomal UDP-glucuronyltransferase activity Sweeny,D.J.;Reinke,L.A.
  35. FEBS Lett. v.181 Inhibitors of distinction of three types of human glutathione transferase Tahir,M.K.;Guthenberg,C.;Mannervik,B.
  36. Toxicol. Lett. v.29 Comparison of in vitro methods and in vivo metabolism of lindane for assessing the effect of repeated administration of ethanol on hepatic drug metabolism Trela,B.A.;Carlson,G.P.;Chadwick,R.W.;Copeland,M.F.
  37. Anal. Biochem. v.86 Determination of malonaldehyde precursor in tissues by thiobarbituric acid test Uchiyama,M.;Mihara,M.
  38. Toxicol. Lett. v.52 Glutathione conjugation in the isolated perfused rabbit lung: The effects of carbon monoxide, buthionine sulfoximine and cibacron blue Yang,C.M.;Carlson,G.P.
  39. Toxicology v.62 Influence of ethanol on glutathione-S-transferase activity and glutathione content in the isolated perfused rabbit lung Yang,C.M.;Carlson,G.P.
  40. Pharmacology v.42 Glucuronidation and sulfation in subcellular fractions and in the isolated perfused rabbit lung: Influence of ethanol Yang,C.M.;Carlson,G.P.
  41. Biochem. Pharmacol. v.41 Effect of ethanol on glutathione conjugation in rat liver and lung Yang,C.M.;Carlson,G.P.
  42. Pharmcol. Res. Commun. v.12 Glutathione S-transferase activities in rat liver: Effect of some factors influencing the metabolism of xenobiotics Younes,M.;Schlichting,R.;Siegers,C.P.
  43. Biochem. Biophys. Res Commun. v.111 Ethanol as an inducer of UDP-glucuronyltransferase: A Comparison with phenobarbital and 3-methylcholanthrene induction in rabbit hepatic microsomes Yost,G.S.;Finley,B.L.