BrdU에 의한 DNA

  • Published : 1991.06.01

Abstract

Complexities of testis structure and function are emphasized in morphometrical and genotoxic evaluation by statistical analysis. F-344 rats were treated with azinphos methyl, cyclophosphomide, and dichlorvos. And Brdu was injected with intrapertionially before sacrifice. The existence and degree of DNA damage were measured by Brdu labeling index which represented relative amount of Brdu incorporated in DNA, morphometric change was evaluated by the relative length of tubular diameter in circular seminiferous tubules and the number of spermatogonia per Sertoli cell in stage IX seminiferous tubules.

Keywords

References

  1. Nature v.240 Mutagenicity of dichlorvos Ashwood-Smith,M.J.;Trevino,J.;Ring,R.
  2. J. Androl. v.7 Antimitotic drugs (cyclophosphomide and vinblastine) in the male rat death and behavioral abnormalities in the offspring Auroux,M.R.;Dulioust,E.M.;Nawar,N.Y.;Yachub,S.G.
  3. Fundam. Appl. Toxicol. v.5 Potential indicators of reproductive toxicity: testicular sperm production and epididymal sperm number, transit time, and motility in F-344 rats Blazak,W.F.;Ernst,T.L.;Stewart,B.E.
  4. Anticancer Res. v.8 Application of ELISA procedure for the quantitation of bromodeoxyurdine incorporated into cellular DNA Cheitlin,R.A.;Bodell,W.J.
  5. Am. J. Anat. v.93 Renewal of spermatogonia of rat Clermont,Y.;Leblond,C.P.
  6. Arch.Toxicol. v.30 The effects of Dichlorvos on clutured human lymphocytes Dean,B.J.
  7. Fundam.Appl. Toxicol. v.6 Use of quantitative testicular histology to assess the effect of dibromocholoropropane (DBCP) on reproduction in rabbits Foots,R.H.;Berndston,W.E.;Rounsaville,T.R.
  8. Mut. Res. v.37 Inhibition of mouse testicular DNA synthesis by mutagens and carcinogens as a potential simple mammalian assay for mutagenesis Friedman,M.A.;Staub,J.
  9. Science v.218 Monoclonal antibody to 5-bromo-and 5-iododeoxyuridine: a new reagent for detection of DNA replication Gratzner,H.G.
  10. Cancer Res. v.42 Comparison of the mutagenicity and teratogenicity of cyclophosphomide and its active metabolites, 4-hydroxycyclophosphamide, phosphoramide mustard, and acrolein Hales,B.F.
  11. Cancer Res. v.48 Analysis of the chromosomal DNA replication pattern using the bromodeoxyuridine labeling method Karube,T.;Watanabe,S.
  12. Bull. Environ. Toxicol. v.11 Alterations of the seminiferous epithelium and the leydimg cells of the rat testis after the application of dichlorvos(DDVP) Kruse,W.K.;Homola,S.
  13. Bull. Environ. Toxicol. v.15 Damage to spermatogenesis in juvenile rat treated with DDVP and malathion Krause,W.;Hamm,K.;Weissmuller,J.
  14. Toxicol. Lett. v.2 Sister chromatid exchange frequences in cultured human cells exposed to an organophosphorus insecticide Nicholas,A.H.;Vienne,M.;Berghe,H.V.D.
  15. Nature v.265 Rapid test to detect agents that damage human DNA Painter,R.B.
  16. Cancer Res. v.48 Cell kinetics in human malignancies studies with in vivo administration of bromodeoxyuridine and flw cytometry Riccardi,A.;Danova,M.;Wilson,G.;Ucci,G.;Dormer,P.;Mazzini,G.;Brugnatelli,S.
  17. Int. J. Cancer. v.23 Carcinogenic action of low-dose cyclophosphomide given orally to Sprague-Dawley rats in a lifetime experiment Schmahl,D.;Habs,M.
  18. Mut. Res. v.46 Inhibition of testicular DNA synthesis by chemical mutagens and carinogens. Preliminary results in the validation of a novel short term test Seiler,J.B.
  19. Histrochem v.85 Immunohistochemical detection of bromodeoxyuridine in formalin-fixed tissues Sugihara,H.;Hattori,T.;Fukuda,M.
  20. Jpn. J. Cancer Res. v.78 Patterns of epithelial proliferation revealed by continuous administration of bromodeoxyuridine during urinary bladder carcinogenesis in rats Tatematsu,M,;Fukushima,;Aoki,T.;Mera,Y.;Inouse,T.;Ito,N.
  21. Mut. Res. v.78 Sister chromatid exchanges and chromosomal aberrations in cultured chinese hamster cells treated with pesticides posticides positive in microbial reversion assays Tezuk,H.;Ando,N.;Suzuki,R.;Terahata,M.;Moriya,M.;Shirasu,Y.
  22. Mut. Res. v.16 On the motagenic action of Dichlorvos Vooged,C.E.;Jacobs,J.J.J.A.A.;van der Stel.
  23. Mut. Res. v.32 Mutagenicity studies of organophosphorus insecticides Wild,D.
  24. Principles and methods of toxicology Assessment of male reproductive toxicity.: A risk assessment approach Zenick,H.;Clegg,E.D.;Hayes,A.W.(eds.)