• Journal of Technologic Dentistry
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• v.23 no.1
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• pp.95-106
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• 2001

The purpose of this study was to show the method of three-dimensional morphometry developed recently and to compare the accuracy of three-dimensional morphometry with those of PA cephalometry, The three-dimensional morphometry analysis program and device were developed. Steel balls (1.2mm in diameter) were attached in twenty five landmarks of artificial human skull. This artificial human skull was used as experimental materials. From three-dimensional morphometry and PA cephalometry of artificial human skull. eleven linear measurements were acquired and made into asymmetry index. Right-left differences of measurements were used as asymmetry index. These measurements and asymmetry index were compared respectively with those of actual. The results were as follows: 1. Mean difference between three-dimensional morphometry and actual artificial human skull in linear measurements was $1.99{\pm}0.37mm$, and mean difference between PA cephalometry and actual was $21.12{\pm}0.45mm$. Both of all were reduced more than those of actual. 2. Mean difference between three-dimensional morphometry and actual artificial human skull in asymmetry index was $0.07{\pm}0.42$, and mean difference between PA cephalometry and actual was $3.63{\pm}0.60$. Three-dimensional morphometry was reduced while PA cephalometry was magnified more than that of actual. 3. Each eleven asymmetry index of three-dimensional morphometry was the same negative sign as those of actuals while only N-Z, ANS-J, Tr-Go, Tr-ANS asymmetry index were the same in PA cephalometry. These results suggest that the method of three-dimensional morphometry is more accurate than those of PA cephalometry in asymmetry analysis.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6919-6922
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• 2014

Background: The cytological analysis of serous effusions is a common investigation and yields important diagnostic information. However, the distinction of reactive mesothelial cells from malignant cells can sometimes be difficult for the cytopathologist. Hence cost-effective ancillary methods are essential to enhance the accuracy of cytological diagnosis. The aim of this study was to examine the utility of nuclear morphometry in differentiating reactive mesothelial cells from malignant cells in effusion smears. Materials and Methods: Sixty effusion smears consisting of 30 effusions cytologically classified as malignant (adenocarcinomas) and 30 benign effusions showing reactive mesothelial cells were included in the study. ImageJ was used to measure the nuclear area, perimeter, maximal feret diameter, minimal feret diameter and the circularity. A total of ten representative cells were studied in each case. Results: Significant differences were found between benign and malignant effusions for the nuclear area, perimeter, maximal feret diameter and minimal feret diameter. No significant difference was found for circularity, a shape descriptor. Receiver operating characteristic (ROC) curve analysis revealed that nuclear area, perimeter, maximal feret diameter, and minimal feret diameter are helpful in discriminating benign and malignant effusions. Conclusions: Computerised nuclear morphometry is a helpful ancillary technique to distinguish benign and malignant effusions. ImageJ is an excellent cost effective tool with potential diagnostic utility in effusion cytology.

• The Korean Journal of Cytopathology
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• v.6 no.2
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• pp.99-105
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• 1995

Morphometry of nuclei of the benign and malignant prostatic lesions was performed to study the relationship between nuclear size and shape and the prognosis of prostatic adenocarcinoma fifty one cases of prostatic adenocarcinoma and 13 cases of benign prostatic hyperplasia were included to evaluate area, perimeter, Dmax, Dmin, and 5 form factors of the nuclei by image analyzer(Zeiss Ibas 2000) using hematoxylin-eosin stained slides. All analytic factors of nuclear size and shape were significantly different between benign lesions and adenocarcinomas. Increased nuclear size was associated with nuclear irregularity, presence of metastasis, advanced clinical stage, and high Gleason's grade and score of prostatic adenocarcinoma. On Kaplan-Meier method, survival was decreased with older age, no hormonal treatment, stage D, high Gleason's grade and stage as well as with larger size and irregular shape of the nuclei in conclusion, morphometry of nuclei of the prostate can be a helpful tool to differentiate between be nign and malignant lesions. Nuclear morphology is thought to be associated with prognosis of prostatic adenocarcinoma.

• Journal of Technologic Dentistry
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• v.27 no.1
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• pp.27-39
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• 2005

This study is to show the qualitative analytic methods of facial asymmetry with three-dimensional morphometry and find out asymmetry change resulted from enlarging three local regions. Steel balls (1.2mm in diameter) were attached in twenty seven landmarks of a symmetrical artificial human skull. This artificial human skull was used as experimental materials. Twelve different asymmetrical artificial human skulls were formed by gradually enlarging the mandibular body length, gonial angle, and ramus height of the left hemiface. From the three-dimensional morphometry of each skull type, nine local area measurements and three total sum area measurements(representing the mandibular area, maxillary area, and lower facial area) were acquired and made into the surface area asymmetry degree. Menton deviation itself was used as the surface area asymmetry degree while right-left percentages were used in the other measurements. These surface area asymmetry degrees were compared with each other to find out asymmetry change according to the degree of actual facial asymmetry. Through the statistical analysis, following results were obtained. The results were as follows: 1. Left maxillary area of artificial human skull was 7.13$\pm$0.26% larger while mandibular area was 4.14$\pm$0.12% smaller than each those of right hemiface. After all, left lower facial area was 1.44$\pm$0.07% larger than those of right hemiface.(n=7). 2. Among the reduce rates of surface area asymmetry degree resulted from enlarging three local regions, ramus height was similar to mandibular body length while it was bigger than those of gonial angle. 3. Among the increase rates of menton deviation resulted from enlarging the local regions, ramus height was the biggest, mandibular body length was the second and gonial angle was the smallest. These results suggest that three-dimensional morphometry can be used to qualitatively analyse facial asymmetry and the asymmetry degree is more influenced by enlarging the ramus height, mandibular body length than those of gonial angle.

• Korean Journal of Psychosomatic Medicine
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• v.22 no.2
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• pp.130-137
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• 2014

Objectives : Obsessive-compulsive(OC) symptoms have yet to be directly studied in neurodegenerative conditions involving behavioral changes. To examine regional abnormalities in the brains of dementia patients with OC symptoms, we assessed the gray matter density using voxel-based morphometry(VBM). Methods : We performed brain magnetic resonance imaging(MRI) with VBM analysis in 106 dementia patients with OC behaviors. In this study, OC behaviors were investigated in patients with neurodegenerative disease using the modified Manchester Behavior Questionnaire. Results : The OC behavior scores were correlated with structural brain volume using VBM. The total OC symptom score correlated negatively with the volume of both putamens, the right middle orbitofrontal gyrus, both anterior cingulate cortices, and the left insula(p<0.001, uncorrected). No gray matter reductions were associated specifically with the OC symptom sub-categories. Conclusions : Our results suggest that abnormalities in these brain regions may play an important role in the pathophysiology of OCD in neurodegenerative disease. This is the first lesion study to investigate the neural basis of OCD behaviors in neurodegenerative disease.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2006.08a
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• pp.146-146
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• 2006

• Proceedings of the Korean Society of Medical Physics Conference
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• 2006.08a
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• pp.149-149
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• 2006

• Imaging Science in Dentistry
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• v.37 no.1
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• pp.27-33
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• 2007

Purpose : The research was done to investigate the effectiveness of 2D bony morphometry and microstructure of micro-computed tomography (micro-CT) on the osteoporotic bony change. We performed the bone morphometric analysis of proximal femur in ovariectomized rabbits with BMD and micro-CT examination. Materials and Methods : Twenty-one female (Newzealand, about 16 weeks old, 2.9-3.4kg) rabbits were used. Three rabbits were sacrificed on the day when experiment began (Baseline). The remaining 18 rabbits were divided into two groups. One group was ovariectomized bilaterally (OVX) and the other animals were subjected to sham operation (Sham). Bone specimens were obtained from the right and left femur of sacrificed rabbits. At intervals of 1, 2, 3, 5, 6 months respectively, BMD tests were performed on the proximal femur by using PIXlmus 2 (GE Lunar Co. USA), 2-dimensional bone morphometric analysis by custome computer program and 2D/3D bone structure analysis by micro-CT (Skyscan 1072, Antwerpen, Belgium). Statistical analysis was carried out for the correlation between bone morphometry, micro-CT and BMD Result : BV/TV, Tb.Th, Tb.N of micro-CT parameters showed higher values in sham group than OVX group. N.Nd/Ar.RI, N.NdNd, N.NdTm, N. TmTm, PmB/Ar.RI, 3-D BoxSlope of 2D morphometric parameters showed higher values in Sham group than OVX group. The micro-CT parameters of Tb.Sp, Tb.N were statistically significant correlated with BMD respectively. Several 2D morphometric parameters were statistically significant correlated with BMD respectively. Conclusion : Several parameters of 2D bony morphometry and micro-CT showed effective aspects on the osteoporotic bony change.

• The Korean Journal of Cytopathology
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• v.9 no.2
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• pp.161-168
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• 1998

Primary breast lesions diagnosed by fine needle aspiration cytology, confirmed by histologic examination were analyzed by morphometry to evaluate the difference between benign and malignant lesions, and the methods obtaining the sample. four size factors and 5 form factors were evaluated in 22 fibroadenomas and 20 carcinomas by image analyzer(Zeiss Ibas 2000) using the H-E stained slides. Nuclear size was significantly larger in the carcinoma cells than fibroadenoma cells both in the cytology and biopsy specimens, but the form factors were not significantly different. Both fibroadencma and carcinoma cells were significantly larger in cytologic smear than histologic section. The cells in the cytology were more regular and round than those in histology, but not statistically significant. Fibroadenomas having cellular proliferation and atypism exhibited larger size and more irregular nuclei than non-proliferative fibroadenoma, but not statistically significant. Therefore nuclear morphometric analysis can be a helpful method to diagnose the questionable breast lesions and is a method appropriate for use as a quality control procedure in the fine needle aspiration cytology.

• Investigative Magnetic Resonance Imaging
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• v.19 no.1
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• pp.10-18
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• 2015

Background and Purpose: The association between the low emotional regulation and the brain structural change of major depressive disorder (MDD) has been proposed, but the voxel-based morphometry (VBM) studies on female MDD are rare. The purpose of the present study was to show the regional volume changes of gray matter (GM) in female patients with MDD by optimized VBM. Methods: To control subjects homogeneity, twenty female MDD patients and age, sex matched 21 normal controls were included for the VBM analysis. To identify the change of regional gray matter volume (GMV), the optimized VBM was performed with T1 MRIs. The amounts of gray/white matter and intracranial cavity volumes (ICV) were measured. The analysis of covariance (ANCOVA) and partial correlation analyses covariate with age and ICV were applied for VBM. Results: The age and ICV distributions were similar between the two groups. In the ANCOVA, the total GMV of MDD was smaller than that of normal controls. In the VBM, regional GMV was relatively decreased in the limbic system (amygdalae, ambient gyri, hippocampi heads, subiculum, posterior parahippocampal gyri, pulvinar nuclei, dorsal posterior cingulate gyri, and left pregenual cingulate gyrus). The lingual gyri, short insular gyri, right fusiform gyrus, and right inferior frontal gyrus were also showed decreased regional GMV. Conclusion: The results of this study indicate that the female MDD is mainly associated with the structural deficits of the limbic system and limbic system related cortices, which were known to the center of emotions.