Proceedings of the KIEE Conference (대한전기학회:학술대회논문집)

The Korean Institute of Electrical Engineers (대한전기학회)
권호 표지

Sheathless electrospray ionization with integrated metal emitter on microfluidic device

전기 분무 이온화를 이용한 단백질 질량분석용 마이크로 유체 소자의 제작 및 실험

  • Kim, Min-Su (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Joo, Hwang-Soo (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University) ;
  • Lee, Kook-Nyung (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Byung-Gee (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University) ;
  • Kim, Yong-Kweon (School of Electrical Engineering and Computer Science, Seoul National University)
  • 김민수 (서울대학교 전기 컴퓨터 공학부) ;
  • 주황수 (서울대학교 응용 화학부) ;
  • 이국녕 (서울대학교 전기 컴퓨터 공학부) ;
  • 김병기 (서울대학교 응용 화학부) ;
  • 김용권 (서울대학교 전기 컴퓨터 공학부)
  • Published : 2004.07.14
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Abstract

In this study, sheathless electrospray from PDMS/glass microchips with conducting metal emitter tip is described. A chip-based capillary electrophoresis/mass spectrometry (CE/MS) system has advantages of the CE separation and on-line electrospray detection of peptide solution. We have fabricated a new electrospray ionization(ESI) device composed of the metal emitter tip and CE separation channel monolithically in a glass microchip. The separation channel and metal emitter tip are fabricated using a glass wet etching and gold electro plating process, respectively. The fabricated micro electrospray chip was tested by spraying peptide sample for mass spectrometric analysis. Singlely-charged peak and doublely-charged peak of peptide were detected and further MS/MS fragmentation was performed in each peak. Direct comparisons with conventional glass or fused silica emitters showed very similar performance with respect to signal strength and stability.

Keywords