Characterization of 2-hydroxymuconic semialdehyde dehydrogenase from Burkholderia cepacia G4

  • A. Matta Reddy (College of Pharmacy, Chungbuk National University) ;
  • Min, Kyung-Rak (College of Pharmacy, Chungbuk National University) ;
  • Kim, Young-Soo (College of Pharmacy, Chungbuk National University)
  • Published : 2003.04.01

Abstract

2-Hydroxymuconic semialdehyde dehydrogenase catalyzes the conversion of 2-hydroxymuconic semialdehyde (HMS) to an enol form of 4-oxalocrotonate which is a step in the catechol-meta cleavage pathway. A tomC gene encoding 2-HMS dehydrogenase of Burkholderia cepacia G4, a soil bacterium that can grow on toluene, cresol, phenol or tricholoro ethylene, is identified in between catechol 2,3-dioxygenase gene and HMS hydrolase gene, its sequence is analysed and the enzyme is characterised. (omitted)

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