• BMB Reports
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• v.31 no.3
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• pp.221-226
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• 1998

Phosphorylation of phytochrome may play important functional roles to control plant photomorphogenesis. Many attempts have failed to identify the protein kinase that phosphorylates phytochrome in vivo. It has been reported that a polycation-stimulated protein kinase activity was associated with the purified phytochrome. However, it is not known if the kinase activity is an intrinsic property of phytochrome or whether it comes from a contaminant of the purified phytochrome. In the present study, three protein kinases that phosphorylate phytochrome have been identified from etiolated oat seedlings. A polycationstimulated protein kinase that had very similar enzymatic properties with that associated with the purified phytochrome was identified in the cytosolic extract. It phosphorylated several contaminant proteins in the kinase preparation as well as phytochrome and had a broad substrate specificity. A CK II-type protein kinase phosphorylated phytochrome and the exogenously added casein. It is likely that this kinase may not be a feasible candidate for the kinase phosphorylating phytochrome in vivo since the content of the kinase seemed to well exceed the content of phytochrome in the etiolated oat seedlings. Another protein kinase that had unique enzymatic properties phosphorylated phytochrome very specifically and seemed to be present in a small quantity in the etiohlted seedlings. It is expected that one of three kinases may be responsible for the phytochrome phosphorylation in vivo.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.5
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• pp.325-331
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• 2005

Seedlings of two rice genotyopes, cvs. Ilpumbyeo and Gancheokbyeo, were exposed to 0, 50 and 100 mM NaCl in nutrient solution for nine days. Plants were collected at the interval of 3 days and organic and inorganic solutes in leaves and roots and antioxidative enzyme activity in leaves were determined. Under salinity, the accumulation of soluble sugars occurred considerably in the older leaves of stressed seedlings compared to younger leaves and roots. The endogenous Na+ contents markedly increased at higher NaCl concentration in leaves and roots of seedlings, though it was higher accumulated in roots. Salinity resulted in an excessive proline accumulation in the stressed plants. A more pronounced increase was observed in Gancheokbyeo leaves. SOD activity in Impumbyeo cannot found any remarkable change, whereas, in Gancheokbyeo, its activity was rapidly decreased. CAT and POD activities increased with an increase in NaCl concentration in both genotypes. In sum­mary, the high capacity of rice seedlings to overcome an unfavorable growth condition such salt stress appears to be related to an adequate partition of organic solutes between shoots and roots and to changes in absorption, transport and re-translocation of salts.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.3
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• pp.220-226
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• 2005

We investigated the effect of $O_3$ on the photosynthetic characteristics of oriental plane (Platanus orientalis L.) that is used as a side tree or ornamental tree in Korea. Two-year-old oriental plane seedlings were transplanted to pots and transferred into a closed $O_3$ chamber, Photosynthetic pigment content and photosynthetic characteristics of leaves were measured every three weeks during 100 ppb $O_3$ fumigation. There was no visible foliar injury by $O_3$ exposure and the content of photosynthetic pigments did not show significant differences between control and $O_3$-treated seedlings. But photosynthetic rate, stomatal conductance, and water use efficiency in leaves of $O_3$-treated seedlings were reduced after six weeks of ozone fumigation. In addition, reduction of carboxylation efficiency and photochemical efficiency was observed in leaves of $O_3$-treated seedlings after three weeks and six weeks. In accordance with our results, carboxylation efficiency, the most sensitive parameter to $O_3$ stress, was considered to be a suitable indicator of $O_3$ sensitivity.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.781-790
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• 2019

Changes in the physical and chemical properties of soil materials during the nursing process have a great influence on the quality of containerized seedlings and on growth and survival after planting. In this study, the effect of biomaterials and their mixed ratios on the growth of Zelkova serrata seedlings in a containerized seedling production system was investigated. Mushroom sawdust, pine bark, and carbonized rice husk were used as biomaterials. The mixed ratios were 10% and 20% volume ratio of the growing medium volume, including the untreated controls. There was no significant difference in the height growth of the Zelkova serrata seedlings according to the biomaterials. The root collar diameter was the highest with the 20% carbonized rice husk and the lowest with the mushroom sawdust. The difference between the highest quality index and the lowest quality index was 30% in the order of the carbonized rice husk, pine bark, control, and mushroom sawdust, but there was no statistical significance. In this study, if the growing medium mixed with biomaterials does not reduce the seedling growth compared with the control, it is considered that the biomaterial can replace a part of the growing media. Therefore, the results show that some of the growing media can be replaced with carbonized rice husk or pine bark when producing Zelkova serrata seedlings.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.247-253
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• 1995

The herbicide butachlor [N-(butoxymethyl)-2-chloro-N-(2,6-di-methylphenyl) acetamide] is widely used by farmers as a tool for weed management of transplanted rice(Oryza sativa L.) in Taiwan. The herbicide did not stop germination of rice and weed seeds, but strongly inhibited the subsequent growth of young shoots and roots. The inhibition was also strong on established seedlings. However, they could recover to normal growth after the herbicide effect disappeared. Butachlor greatly decreased the endogenous indole-3-acetic acid (IAA) but increased the endogenous abscisic acid (ABA) contents of rice seedlings. Addition of lAA into growth medium (Hoagland's solution) partly relieved growth inhibition. Pretreatment of both gibberellic acid ($GA_3$) and IAA 24 hours before butachlor treatment almost completely alleviated the butachlor-interfere with GA and/or IAA metabolism or their action resulting in the growth inhibition of rice. Butachlor was readily absorbed by rice roots. During 24 hours of uptake experiment, 32% of the applied herbicide was absorbed. Pretreatment of the herbicide for 2 days did ncx affect the absorption. Of the absorbed herbicide, 80% remained in roots, only 20% transported into shoots, and more than 50% was metabolized to water soluble substances. Thin-layer chromatographic (TLC) analysis indicated that the Rf value of the most abundant metabolite was butachlor-glutathione conjugate. Rice, barnyardgrass (Echinochloa crus-galli (L.) Beauv.), and monochoria (Monochoria vaginalis Presl) seedlings contained relatively high level of non-protein thiols, while the glutathione S-transferase (GST) activity was found highest in rice, barnyardgrass the next, monochoria the lowest. The difference in GST activity among these species might be related to their sensitivity to butachlor.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.1
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• pp.12-17
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• 2002

This study identified the physiological characteristics of Alnus firma seedlings collected from air polluted industrial complex of Yeosu area with inoculation of nitrogen-fixing nodule. A. firma individuals resistant or sensitive to air pollution stress at the study area were selected. Seeds of the individuals were sown in a pot and inoculated with the nodules collected at the same area about 3 weeks after germination. To compare the inoculation effects, photosynthesis was measured among the A. firma seedlings under 12 inoculation combinations of seed sources and nodule sources. Photosynthetic activities of seedlings from resistant mother trees were obviously higher than those from sensitive mother trees. In general, the seedlings inoculated with nodule of sensitive trees, showed a worse physiological trend on every combination. In this study, inoculation effect was less important factor than seed sources to photosynthetic activities of A. firma. This physiological characteristic seemed to be determined by seed sources rather than nodule sources.

• Korean Journal of Environment and Ecology
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• v.37 no.1
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• pp.13-34
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• 2023

Many ecologists try to understand plant communities and societies' changing processes by interacting with environmental factors. Plant communities formed in the sidings of streams, which are more dynamic than forest vegetation, are closely related to the disturbance regime of streams. U. pumila, which forms communities at the edges of streams, is distributed over the central and northern regions of the Korean Peninsula. In this study, we intended to explain the regeneration of U. pumila communities in the upstream region of the Namhangang River. Heights from the stream surface to the mid-high points of communities increased when the age class of U. pumila communities increased. On the other hand, the height of U. pumila tended to increase and decrease as the age class increased. The richness (S), evenness (J'), diversity (H'), and maximum diversity (H'max) also showed a tendency to increase and then decrease as the age class increased. On the other hand, the number of stems per individual and density (D) decreased as the age class increased. Seedlings of U. pumila failed to regenerate in the communities at the intermediate or higher stage of the age class. Regeneration of seedlings occurred in the spaces formed by the flooding, which occurred periodically in streams. After regeneration, U. pumila communities grew like a single organism. On the other hand, the plant species consisting of U. pumila communities exhibited a process in which the S and H' increased and then decreased as they developed. In other words, we determined that the changes in the species compositions were associated with changes in each species' environment and community. A space where seeds can regenerate seedlings should be provided for the regeneration of U. pumila communities. In the mid/upstream region of the Namhangang River, where the present study focused, newly formed communities were rare because the space required to regenerate U. pumila ceased to exist. Accordingly, we suggest securing an appropriate space for the regeneration of seedlings to maintain the U. pumila communities in the upstream region of the Namhangang River.

• Journal of Bio-Environment Control
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• v.10 no.3
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• pp.159-164
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• 2001

This study were conducted to investigate the influence of chilling on photosynthetic rate, root activity, contents of total sugars and fatty acids of cucumber seedlings grown in a greenhouse. Even though photosynthetic activity of seedlings exposed to $0^{\circ}C$ for 5 hours was little or insignificantly influenced, it was reduced by 52.8% and 67.7% in seedlings exposed to the same temperature for an extended 10 and 24 hours, respectively. Photosynthetic rate decreased significantly when seedlings were illuminated, as compared to continuously held under darkness, during 15 hours of chilling treatment at 3$^{\circ}C$. Recovery of photosynthetic ability was also retarded by illumination during a recovery period after chilling treatment. Root activity, as measured by the oxidation power of $\alpha$-naphtylamine, was significantly reduced by chilling treatment at 0 to 6$^{\circ}C$, but amount of bleeding xylem sap collected at 40 days after chilling treatment was not significantly different among treatments. Total sugar content increased by 12 and 23% as compared to the control in seedlings chilled for 24 hours, respectively, at 3$^{\circ}C$. Contents of unsaturated linolenic and oleic acids increased, while content of saturated palmitic acid decreased with chilling treatment.

• Journal of Ecology and Environment
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• v.41 no.6
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• pp.152-164
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• 2017

Background: Natural regeneration of seedlings as well as saplings of Korean fir has been significantly impacted by the browsing from the early stages of their development, potentially, by roe deer for the last two to three decades at the study site since late 1980s. This study was carried out to investigate current status of the disturbance in the seedling development of Korean fir (Abies koreana) on Mt. Hallasan, Jeju Island, Korea. Methods: Field survey was carried out during June and August in 2016 to measure the characteristics of study site and understory vegetation by applying systematic sampling to 125 plots of $5m{\times}5m$ quadrat located on eastern slope of the mountain. Correlation and regression analyses were applied to the variables quantified from the data sets using the SAS software. Results: No saplings with their diameters at breast heights smaller than 5.0 cm were found at the study site indicating the serious disturbance in the natural regeneration of Korean fir at the study site. No seedlings with their heights taller than 36.0 cm were found at the study site indicating even more serious disturbance during earlier stage of the natural regeneration of Korean fir at the study site. A total of 616 individuals of the seedlings of Korean fir were found at 54 out of 125 sampling plots. One hundred thirty-eight seedlings (22.4%) out of 616 individual seedlings have the vestiges for being grazed, potentially, by roe deer. Conclusions: Due considerations should be given to the effects of browsing of the seedlings by roe deer to promote the natural regeneration of Korean fir, ultimately to restore Korean fir. It is needed for the managers of the forest to install fences around the forest area. Exclosure experiments as well as enclosure experiments of different densities of browsing should be carried out. In addition, treatment with different densities of Jeju dwarf bamboo should also be included in the experiment on Mt. Hallasan National Park, Jeju Island, Korea.

• Food Science and Preservation
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• v.10 no.3
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• pp.365-369
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• 2003

The main objective of this study was to investigate the pattern of glucosinolates, in terms of 3-butenyl isothiocyanate, and total glucosinolates, in different parts of young seedlings and seeds in Korean Chinese cabbages. For determination of glucosinolates, 55 days and Winter pride cultivars of Korean Chinese cabbages seeds and different parts of 1, 3, 5, and 7-day-old seedlings were used for analytical sample preparation provided with an anion exchanges column, and measured by GC and UV-visible Spectrophotometer. The concentration of 3-butenyl ITC was higher in the cotyledon and the hypocotyl parts of 55 days cultivar seedling than those of Winter Pride cultivar. In the cotyledon parts of Winter pride cultivar seedling, 3-butenyl ITC amount was increase to 3-day-old seedling and then reduced. The cotyledon of 55 days cultivar contained the highest concentration of total glucosinolates while those were increased in the hypocotyl and decreased in the root. The level of total glucosinolates was increased to 5-day-old seedling and then decreased in the cotyledon and the hypocotyl parts of seedling in Winter pride cultivar. There was no significant difference of total glucosinolates in the root part. In the seeds, 55 days cultivar concentration was higher than Winter pride cultivar both of 3-butenyl ITC and total glucosinolates. In our study, the levels of 3-butenyl ITC and total glucosinolates depended on cultivars, growth stages, and parts in Korean Chinese cabbage.