• Title/Summary/Keyword: yield conditions

Search Result 3,129, Processing Time 0.034 seconds

The Preparation of Alkenyl Fluorides form Organometallic Reagents

  • 이승한;Martin Riediker;Jeffrey Schwartz
    • Bulletin of the Korean Chemical Society
    • /
    • v.19 no.7
    • /
    • pp.760-766
    • /
    • 1998
  • Fluorination of alkenyllithium reagents can be accomplished in acceptable yield under conditions which give rise to low competitive alkene formation. These reactions are abetted by the use of the low temperature soluble, mild fluorinating agent N-fluoro-N-tert-butylbenzenesulfonamide; "simpler" fluorinating reagents such as F2, XeF2 or FClO3 failed to give acceptable amounts of the fluoroolefin with these alkenyllithiums.

Optimization of Extraction Conditions for Lyophyllum ulmarium by Response Surface Methodology (반응표면분석에 의한 만가닥 버섯의 추출조건 최적화)

  • 김현구;최맑음;김미옥;김공환
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.32 no.4
    • /
    • pp.574-580
    • /
    • 2003
  • Optimal extraction conditions for yield, browning color, electron donating ability, nitrite scavenging effect, total polyphenol content and tyrosinase inhibitory activity of Lyophyllum ulmarium were determined by using response surface methodology (RSM) through the central composite design. The extraction yield of Lyophyllum ulmarium was effected by ethanol concentration and browning color was improved with the increase of ethanol concentration than microwave power. The nitrite scavenging effect was improved with the increase of ethanol concentration and decrease of microwave power The electron donating ability, browning color, tyrosinase inhibitory activity and total polyphenol content were improved with the increase in ethanol concentration and microwave power. The optimal ranges of extraction conditions for effective components of Lyophyllim ulmarium were predicted as 60.05~102.75 watt of microwave power, 53.20~64.01% of ethanol concentration and 7.77 min of extraction time.

Change of Antioxidant Activity and Antioxidant Compounds in Saururus chinensis by Extraction Conditions (추출조건에 따른 삼백초 추출물의 항산화 활성 및 성분의 변화)

  • Kim, Suk-Kyung;Ban, So-Youn;Kim, Jun-Sung;Chung, Shin-Kyo
    • Applied Biological Chemistry
    • /
    • v.48 no.1
    • /
    • pp.89-92
    • /
    • 2005
  • This study was conducted in order to establish the optimum extraction conditions in obtaining Saururus chinensis extracts. At the optimum extraction solvent (40% ethanol solvent), yield, DPPH, total phenolic compounds and total flavonoid in the extract of Saururus chinensis were 13.50%, 83.50%, 2.60 mg/ml and 2.09 mg/ml, respectively. At the optimum extraction time (4 hours), yield, DPPH, total phenolic compounds and total flavonoid in the extract of Saururus chinensis were 12.79%, 71.13%, 2.83 mg/ml and 2.16 mg/ml, respectively. At the optimum conditions (40% ethanol solvent and 4 hours), quercetrin and quercetin contents were 360.13 mg/100 g and 1379.54 mg/100 g, respectively. From the above results, we suggest that the optimum Saururus chinensis extract conditions are 40% ethanol solvent and 4 hours.

Formation of Succinic Acid by Klebsiella pneumoniae MCM B-325 Under Aerobic and Anaerobic Conditions

  • Thakker Chandresh;Bhosale Suresh;Ranade Dilip
    • Journal of Microbiology and Biotechnology
    • /
    • v.16 no.6
    • /
    • pp.870-879
    • /
    • 2006
  • The present study describes the formation of succinic acid by a nonvirulent, highly osmotolerant Klebsiella pneumoniae strain SAP (succinic acid producer), its profile of metabolites, and enzymes of the succinate production pathway. The strain produced succinate along with other metabolites such as lactate, acetate, and ethanol under aerobic as well as anaerobic growth conditions. The yield of succinate was higher in the presence of $MgCO_3$ under $N_2$ atmosphere as compared with that under $CO_2$ atmosphere. Analysis of intracellular metabolites showed the presence of a smaller PEP pool than that of pyruvate. Oxaloacetate, citrate, and $\alpha$-ketoglutarate pools were considerably larger than those of isocitrate and fumarate. In order to understand the synthesis of succinate, the enzymes involved in end-product formation were studied. Levels of phosphoenolpyruvate carboxykinase, fumarate reductase, pyruvate kinase, and acetate kinase were higher under anaerobic growth conditions. Based on the profiles of the metabolites and enzymes, it was concluded that the synthesis of succinate took place via oxaloacetate, malate, and fumarate in the strain under anaerobic growth conditions. The strain SAP showed potential for the bioconversion of fumarate to succinate under $N_2$ atmosphere in the presence of $MgCO_3$. At an initial fumarate concentration of 10 g/l, 7.1 g/l fumarate was converted to 7 g/l succinate with a molar conversion efficiency of 97.3%. The conversion efficiency and succinate yield were increased in the presence of glucose. Cells grown on fumarate contained an 18-fold higher fumarate reductase activity as compared with the activity obtained when grown on glucose.

Evaluation for anaerobic germinability of rice germplasm for direct-seeding cultivation under submerged conditions

  • Rauf, Muhammad;Choi, Yu-Mi;Lee, Sukyeung;Lee, Myung-Chul;Oh, Sejong;Hyun, Do Yoon
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2017.06a
    • /
    • pp.71-71
    • /
    • 2017
  • Stable stand establishment is pre-requisite in direct rice seeding system for obtaining optimal yield of rice crop in rain-fed and waterlogged areas. Anaerobic condition on waterlogged soil causes low germination which significantly reduces crop yield. Due to low availability of tolerant genetic material for anaerobic germination, there is urgent need to evaluate rice germplasm for better germinability under anaerobic conditions. Seeds of the 185 rice accessions were evaluated for germination vigor and coleoptile length under anaerobic conditions. The variation among germplasm was tested for significance using analysis of variance and various multivariate components. Significant level of variation was observed among all accessions for germination vigor and coleoptiles length. Although highest mean values for coleoptiles length (2.1cm) and germination rate (60%) were observed in japonica accessions but maximum coleoptile length (4.68cm) and germination rate (96%) was found in indica genotype CO18. A highly significant and positive correlation was also observed between germination vigor and coleoptiles length, which signify the importance of elongated coleoptile under anaerobic conditions. The PCA analysis illustrated that 97.24% variation was accounted by PC1 while PC2 and PC3 explained 2.54% and 0.24% variation for germination vigor and coleoptile length. PCA scattered plot divided the accessions in four various groups. All AG tolerant accessions were included in group I. Likewise in the case of cluster analysis, two major clades (I and II) were formed. All accessions showing >40% germination rate were included in clade I, whereas all other accessions with <40% germination rate were grouped in clade II. Further more highly tolerant accessions (>80% germination) were grouped in sub-cluster IA.

  • PDF

Studies on the Wet-injury Resistance of Wheat and V. Interrelationship among the characters of Roots and Those of Tops in Barley and Wheat Crop (맥류 내냉성에 관한 연구 제5보 맥류근의 생육환경차이가 지하 및 지상부의 형질에 미치는 영향)

  • Hyung-Soo Suh;Rae-Kyung Park
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.24 no.1
    • /
    • pp.66-72
    • /
    • 1979
  • interrelationship among the characters of the top and the root within a plant was investigated by deviding the roots of the plant into two parts and growing each one in two different environmental conditions, The roots grown on the poor environmental conditions such as over flooding moisture and overdried were less in numbers of root. shorter in root length. and lighter in root weight than those of good growing conditions, It was demonstrated that the roots grown under the poor environmental conditions affected not only the growing of the associated tillers but also the growing of all the tillers within a plant. In comparision with untreated plants. the culm length of treated plants was shortened. and the heading time of them was delayed. The most remarkable yield reduction was appeared at the over flooding moisture treatment. and the next was at the overdried treatment. The ten dency of yield reduction was more severe in sandy soil than that of clay loam.

  • PDF

Xylitol Production by Kluyveromyces marxianus 36907-FMEL1 at High Temperature was Considerably Increased through the Optimization of Agitation Conditions (교반속도 최적화를 통한 Kluyveromyces marxianus 36907-FMEL1로 부터 고온에서의 자일리톨 생산성 증진)

  • Kim, Jin-Seong;Park, Jae-Bum;Jang, Seung-Won;Kwon, Deok-Ho;Hong, Eock Kee;Shin, Won Cheol;Ha, Suk-Jin
    • Microbiology and Biotechnology Letters
    • /
    • v.45 no.1
    • /
    • pp.57-62
    • /
    • 2017
  • Recently, we isolated the mutant Kluyveromyces marxianus 36907-FMEL1, which demonstrated improved xylose reductase activity as compared to the parental strain, K. marxianus ATCC 36907. Effects of agitation conditions on xylitol production were verified using a bioreactor system. Under an agitation speed of 400 rpm, K. marxianus 36907-FMEL1 exhibited the highest xylitol yield (0.57 g/g) and productivity ($0.64g{\cdot}l^{-1}{\cdot}h^{-1}$) at $30^{\circ}C$. When the fermentation temperature was increased to $40^{\circ}C$, interestingly, xylitol yield and productivity were found to be increased to 21% (0.64 g/g) and 58% ($0.90g{\cdot}l^{-1}{\cdot}h^{-1}$), respectively, under the optimized agitation conditions.