• Title/Summary/Keyword: yield conditions

Search Result 3,129, Processing Time 0.03 seconds

Protoplast Isolation and reversion from Pleurotus spodoleucus (참느타리버섯의 원형질체(原形質體) 분리(分離) 및 환원(還元))

  • Yoo, Young-Bok;Byun, Myung-Ok;Go, Seung-Joo;You, Chang-Hyun;Chang, Kwon-Yawl
    • The Korean Journal of Mycology
    • /
    • v.15 no.1
    • /
    • pp.19-22
    • /
    • 1987
  • This experiment was undertaken to investigate proper conditions for protoplast formation from Pleurotus spodoleucus. Novozym 234 was the most effective enzyme and a high yield of protoplasts was obtained. Combination of enzymes did not improve this result. Sucrose gave the best result to support the release and maintain the stability of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs. in shaking condition at 120 strokes $min-^1$. When mycelium of P. spodoleucus was cultured for 3 days on mushroom complete agar medium at $27^{\circ}C$, the formation of protoplasts was effective. The mushroom complete agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasts.

  • PDF

Granulite xenoliths in porphyroblastic gneiss from Mt. Jiri area, SW Sobaegsan massif, Korea (소백산육괴 서남부 지리산지역의 반상변정질 편마암에서 산출되는 백립암질 포획암)

    • The Journal of the Petrological Society of Korea
    • /
    • v.8 no.1
    • /
    • pp.34-45
    • /
    • 1999
  • Mafic granulite xenoliths are found in precambrian porphyroblastic gneiss of the Mt. Jiri area, SW Sobaegsan massif, Korea. The xenoliths are rounded to ellipsoidal in shape, 50-100 cm in length and coarse-grained with granoblastic and foliated texture. The xenoliths consist of orthopyroxene, garnet, biotite, plagioclase, quartz, ilmenite and secondary orthoamphibole. Orthopyroxene is mostly resorbed and rimmed by coronitic orthoamphiboles. Garnets occur as porphyblasts and are zoned with higher pyrope content in cores than in rims. Geothermo-barometry results yield conditions of about $800-850^{\circ}C$, 6 kb and $500^{\circ}C$, 4 kb for early and retrograde stages of equilibration, respectively. According to available geochronological data, it is suggested that the granulite facies metamorphism occurred prior to 2.1-1.9Ga and that the area was superimposed by the high-grade (over $600-700^{\circ}C$) metamorphism between 1.9-1.7Ga, followed by cooling during uplift.

  • PDF

Fitness cost and competitive ability of transgenic herbicide-tolerant rice expressing a protoporphyrinogen oxidase gene

  • Chun, Young Jin;Kim, Dae In;Park, Kee Woong;Jeong, Soon-Chun;Park, Sangkyu;Back, Kyoungwhan;Kim, Chang-Gi
    • Journal of Ecology and Environment
    • /
    • v.36 no.1
    • /
    • pp.39-47
    • /
    • 2013
  • The expression of transgenic traits in genetically modified crops is sometimes associated with decreases in crop performance or fitness. These decreases in performance or fitness of transgenic plants in unfavourable conditions may provide valuable information about the ecological consequences of transgene escape. In a glasshouse trial, we tested the cost associated with resistance to herbicides by comparing the growth, yield, and competitive ability of transgenic rice with its parental non-transgenic line. This new line was developed for constitutive overexpression of protoporphyrinogen oxidase (PPO) to increase resistance to herbicides. We evaluated nine agronomic traits of transgenic and non-transgenic rice grown in a replacement series design over four densities. Competitive ability was also assessed between transgenic and non-transgenic plants by analyzing their relative yields based on biomass and seed weight data. Our results indicated that non-transgenic plants showed greater performance than did the transgenic plants when those genotypes were grown in mixtures. The non-transgenic rice plants exhibited superior competitive ability at certain combinations of planting densities and genotype proportions. These results suggest that PPO-herbicide resistance incurs some costs in plant performance and competitive ability.

Basic Study on the in-situ Biogenic Methane Generation from Low Grade Coal Bed (저품위 석탄의 원지반에서의 생물학적 메탄가스 생산에 관한 기초연구)

  • Wang, Fei;Jeon, Ji-Young;Lim, Hak-Sang;Yoon, Seok-Pyo
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.23 no.4
    • /
    • pp.11-20
    • /
    • 2015
  • In the present work, a basic study on the in-situ biogenic methane generation from low grade coal bed was conducted. Lignite from Indonesia was used as a sample feedstock. A series of BMP (Biochemical Methane Potential) tests were carried out under the different experimental conditions. Although nutrients and anaerobic digester sludge were added to the coal, the produced amount of methane was limited. Both temperature control and particle size reduction showed little effect on the increase of methane potential. When rice straw was added to lignite as an external carbon source, methane yield of 94.4~110.4 mL/g VS was obtained after 60 days of BMP test. The calorific value of lignite after BMP test decreased (4.5~12.1 %) as increasing the content of rice straw (12.5~50 wt % of lignite), implying that anaerobic digestion of rice straw led to partial degradation of lignite. Therefore, rice straw could be used as an external carbon source for the start-up of in-situ biogas generation from low grade coal bed.

Evaluation of Characteristics and Reliability of an Auger Crane with Built-in Hydraulic Extender (유압식 확장기가 내장된 오거 크레인의 특성 및 신뢰성 평가)

  • Kim, Jeom-Sik;Kwon, Sin-Won
    • The Transactions of the Korean Institute of Electrical Engineers P
    • /
    • v.59 no.1
    • /
    • pp.77-82
    • /
    • 2010
  • This study evaluated the characteristics and reliability of an auger crane with a built-in hydraulic extender. The field test of the hydraulic extender was performed with the hydraulic lines filled with hydraulic fluid and free of air. The pressure generated during the test was measured with a digital pressure gauge. The crane was considered to have undergone one cycle of the excavation process after it had performed excavation under three conditions at the same location. This process was performed three times in total. From the results of the excavation using the hydraulic extender, it was found that the maximum pressure and torque measured were 19.9 [MPa] and 895.4 [$kgf{\cdot}m$], respectively. The rotation force of the auger crane generated at this time signifies a horizontal force. If the excavation diameter of the auger crane is increased, the rotation speed is reduced causing the circumferential speed to also be reduced. The torsional shear stress of the extendable auger crane was calculated to be approximately 23.5 [MPa]. However, the rotation shaft material used for this system was carbon steel for machine structural use (SM45C). Since the minimum torsional yield stress is greater than 150 [MPa] according to KS D 3752, it means the equipment has secured a safety factor greater than 6. Therefore, it was found that when performing work using the extendable auger crane, it exhibited no problems with the safety and reliability of its shaft.

Mechanical Properties and Microstructure on Dissimilar Friction-Stir-Weld of Aluminium Alloys (FSW된 이종알루미늄합금의 접합 특성 및 미세 조직)

  • Han, Min-Su;Jang, Seok-Ki
    • Journal of Advanced Marine Engineering and Technology
    • /
    • v.35 no.1
    • /
    • pp.75-81
    • /
    • 2011
  • Dissimilar joining of aluminum 6061-T6 alloy to aluminum 5083-O alloy was performed using friction-stir welding technique. The mechanical properties, hardness, macro- and micro-structure on dissimilar friction-stir-weld aluminium alloy were investigated. Mechanical properties of the weld mainly depend on which Al alloy is placed at the retreating sides of the rotating tool respectively during dissimilar friction-stir weld because the microstructure of stir zone was mainly composed of welded Al alloys of the retreating side. Onion ring pattern was observed like lamella structure stacked by each Al alloy in turn. It apparently results in defect-free weld zone that traverse speed was changed to 124 mm/min under conditions of tool rotation speed like 1250 rpm with 5 mm of tool's prove diameter, 4.5 mm of prove length, 20 mm of shoulder diameter, and $2^{\circ}$ of tilting angle. The 231 MPa of ultimate stress and the 121 MPa of yield point are obtained about the friction-stir-welded Al 6061-T6(AS) to Al 5083-O(RS).

Establishment of artifical cultivation technique of Pleurotus ostreatus using an onion net (양파망을 이용한 느타리버섯(Pleurotus ostreatus) 재배기술)

  • Yu, Young-Jin;Seo, Sang-Young;Jung, Gi-Tai;Ryu, Jeong;Ko, Bok-Rai;Choi, Joung-Sik;Kim, Myung-Kon
    • Journal of Mushroom
    • /
    • v.4 no.3
    • /
    • pp.101-105
    • /
    • 2006
  • This was performed to improve the working conditions of oyster mushroom cultivation on bed using rice straw of which binding and cutting procedure need a lot of time. There was a little difference in physiochemical components between rice straw and cotton waste C/N ration of cotten waste was, however, similar to rice straw as 84-85. If is feasible to use as substrate material. The average yield of a net was 1450g in six frustration harvest, when the size of an onion net was $35{\times}30cm$, mixture ratio of rice straw to cotton waste was 40:60(V/V). Cultivation method using an onion net saved 3 days for preparation, 4 hours for inoculation compared to normal bed cultivation. This method was analysed to give 5% economical benefit.

  • PDF

Study on Structural Analysis and Manufacturing of Polyethylene Canoes (폴리에틸렌 카누의 구조해석과 제조에 관한 연구)

  • Park, Chan-Kyun;Kim, Min-Gun;Cho, Seok-Swoo
    • Transactions of the Korean Society of Mechanical Engineers A
    • /
    • v.35 no.3
    • /
    • pp.309-316
    • /
    • 2011
  • Canoes are usually made from wood or FRP. However, today environment-friendly materials are preferred, and hulls made of FRP are prohibited in some countries. Polyethylene can be recycled and so is suitable for synthetic canoe construction. We used 3D Boat-Design to determine the hydrostatic properties of the canoe. Flow-structure coupled analysis was performed using ANSYS Workbench R12.1. The hull pressure and passenger weight were considered as canoe loading factors. The key parameters for the canoe are the design variables. The constraints are as follows: (1) The maximum stress must not exceed 50% of the polyethylene yield stress; and (2) the canoe weight must not exceed 50 kg. The optimal structural conditions were obtained by the response optimization process. The components of the canoe hull were manufactured from polyethylene pipes and joined by thermal fusion methods. Tests showed that the polyethylene canoe had better performance than existing canoes.

Saccharification of lignocellulosics by Supercritical Water (초임계수를 이용한 목질바이오매스의 당화 특성)

  • Choi, Joon-Weon;Lim, Hyun-Jin;Jo, Tae-Su;Han, Gyu-Sung;Choi, Don-Ha
    • New & Renewable Energy
    • /
    • v.3 no.1 s.9
    • /
    • pp.38-45
    • /
    • 2007
  • To characterize thermo-chemical feature of sugar conversion of woody biomass, poplar wood ($Populus\;alba{\times}glandulosa$) powder was treated with supercritical water system. Supercritical water treatment (SCWT) was performed for 60 seconds at different temperatures (subcritical zone 350; supercritical zone $300,\;400,\;425^{\circ}C$) under two pressures $230{\pm}10atm$ as well as $330{\pm}10atm$, respectively, using flow type system. After separation of solid residues from SCWT products, the monomeric sugars in aqueous part converted from poplar wood powder were quantitatively determined by high performance anionic exchange chromatography [HPAEC] equipped with PAD detector and Carbo Pac PA10 column. As the temperature treated increased, the degradation of poplar wood powder was enhanced and ca 83% of woody biomass was dissolved into the water at $425^{\circ}C$. However, the pressure didn't help the degradation of biomass components. At subcritical temperature range, xylose was first formed by degradation of xylan, which is main hemicellulose component in hardwood species, while cellulose degradation started at the transition zone between sub and supercritical conditions and was remarkably accelerated at the supercritical temperature. In the supercritical water system the maximum yield of monomeric sugars amounts to ca. 7.3% based on oven dried wood weight at $425^{\circ}C$.

  • PDF

Groundwater Flow Modeling for a Finite Unconfined Sandy Aquifer in a Laboratory Scale (사질 자유면 대수층 모형에서의 지하수 모델링)

  • 이승섭;김정석;김동주
    • Journal of the Korean Society of Groundwater Environment
    • /
    • v.6 no.4
    • /
    • pp.188-193
    • /
    • 1999
  • Transport of pollutants in aquifer largely depends on groundwater flow which is governed by aquifer hydraulic parameters. Determination of these parameters and associated groundwater modeling become essential for adequate remediation of contaminate groundwater. The objective of this paper is to analyze groundwater flow and determine the optimum hydraulic parameters by performing groundwater modeling based on sensitivity analysis for unconfined sandy gavel aquifer constructed in a laboratory scale under various boundary condition. Results revealed that the simulated drawdown was lower than the observed drawdown irrespective of boundary conditions. and specific yield (S$_{y}$) had less effect on the grondwater flow than permeability (K) in the aquifer. Water balance analysis showed that the measured drawdown in neighboring observation wells during pumping was higher than either simulated or recovered water table. The indicated that a difference might exist in the water tables between aquifer and wells. The difference was investigated by time domain reflectometry (TDR) measurements on water contents in the region of water table and capillary fringe, and explained by a delayed response of water table during gravitational drainage as the water table was lowered as a result of pumping.g.

  • PDF