• Title/Summary/Keyword: yield conditions

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Changes of Total Polyphenol Content and Electron Donating Ability of Aster glehni Extracts with Different Microwave-assisted Extraction Conditions (마이크로웨이브 추출조건에 따른 섬쑥부쟁이 추출물의 총 폴리페놀 함량 및 전자공여 작용 변화)

  • Kim, Hyun-Ku;Kwon, Young-Joo;Kim, Kong-Hwan;Jeong, Yoon-Hwa
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1022-1028
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    • 2000
  • Aster glehni was extracted by microwave-assisted extraction(MAE) and reflux extraction(RE) methods and their extraction efficiencies were compared. A considerable reduction in extraction time was achieved by MAE. When 70% methanol, 50% methanol, 70% ethanol, or 50% ethanol was used, MAE extract contained nearly same amounts of soluble solid and total polyphenol contents as obtained by RE. The optimum MAE conditions for the extraction of Aster glehni were $120{\sim}150$ watts of microwave energy and $4{\sim}8$ minutes of extraction time. No significant changes were found in electron donating ability(EDA) over the variation of microwave energy or extraction time. The use of diluted methanol or ethanol resulted in improving extraction yield(24%), total polyphenol content(2.6%) and EDA(60%).

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Improved Purification of Thermophilic FoF1-ATP Synthase c-Subunit Rings and Solid-State NMR Characterization of Them in Different Lipid Membranes

  • Bak, Suyeon;Kang, Su-Jin;Suzuki, Toshiharu;Yoshida, Masasuke;Fujiwara, Toshimichi;Akutsu, Hideo
    • Journal of the Korean Magnetic Resonance Society
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    • v.17 no.2
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    • pp.67-75
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    • 2013
  • ATP synthase produces ATP, a major energy source for metabolic processes in organisms, from ADP and inorganic phosphate in cellular membranes. ATP synthase is known as a rotary motor, in which the c-subunit ring functions as a rotor. In this work, we have tried to develop a more general preparation procedure of thermophilic $F_oc$-ring ($TF_oc$-ring) for NMR measurements. The expression of $TF_oF_1$ is easily affected by various experimental conditions such as temperature, shape and size of a flask, a volume of medium, and shaking rate of an incubator. Accordingly, we have tried to optimize the expression conditions of $TF_oF_1$. $TF_oc$-rings were purified from $TF_oF_1$ according to a reported method. We modified purification procedures to improve purity and yield of $TF_oc$. On top of them, we found a new combination of detergents for the purification at anion-exchange column chromatography. To examine the effect of lipid environments on the structure, the $TF_oc$-rings were reconstituted into two kinds of lipid bilayers, namely, saturated and unsaturated lipid ones. Then, we have compared characteristics of the $TF_oc$-ring structures in these membranes with solid-state NMR.

Process optimization for biodiesel production from indigenous non-edible Prunus armeniaca oil

  • Singh, Deepak;Kumar, Veerendra;Sandhu, S.S.;Sarma, A.K.
    • Advances in Energy Research
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    • v.4 no.3
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    • pp.189-202
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    • 2016
  • This work emphasized optimum production of biodiesel using non-edible Prunus armeniaca (Bitter Apricot) oil via transesterification collected from the high altitude areas of Himachal Pradesh, India. In this study the author produced biodiesel through the process of transesterification by using an alkali catalyst with alcohol (methanol and ethanol), under the varying molar ratio (1:6, 1:9, 1:12), variable catalyst percentage (1% and 2%) and temperature ($70^{\circ}C$, $75^{\circ}C$, $80^{\circ}C$, $85^{\circ}C$). Furthermore, a few strong base catalysts were used that includes sodium hydroxide, potassium hydroxide, sodium metal and freshly prepared sodium methoxide. After screening the catalyst, response surface methodology (RSM) in connection with the central composite design (CCD) was used to statistically evaluate and optimize the biodiesel production operation using NaOH as catalyst. It was found that the production of biodiesel achieved an optimum level biodiesel yield with 97.30% FAME conversion under the following reaction conditions: 1) Methanol/oil molar ratio: 1:6, 2) Reaction time: 3h, 3) Catalyst amount: NaOH 2 wt. %, and 4) Reaction temperature: $85^{\circ}C$. The experimental results showed that the optimum production and conversion of biodiesel through the process of transesterification could be achieved under an optimal set of reaction conditions. The biodiesel obtained showed appropriate fuel properties as specified in ASTM, BIS and En- standards.

Bio-control of Stem Rot in Jerusalem Artichoke (Helianthus tuberosus L.) in Field Conditions

  • Junsopa, Chutsuda;Saksirirat, Weerasak;Saepaisan, Suwita;Songsri, Patcharin;Kesmala, Thawan;Shew, Barbara B.;Jogloy, Sanun
    • The Plant Pathology Journal
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    • v.37 no.5
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    • pp.428-436
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    • 2021
  • Stem rot is a serious disease in Jerusalem artichoke (JA). To reduce the impact of this disease on yield and quality farmers often use fungicides, but this control method can be expensive and leave chemical residues. The objective of this study was to evaluate the efficacy of two biological control agents, Trichoderma harzianum T9 and Bacillus firmus BSR032 for control of Sclerotium rolfsii under field conditions. Four accessions of JA (HEL246, HEL65, JA47, and JA12) were treated or notreated with T. harzianum T9 and B. firmus BSR032 in a 4 × 2 × 2 factorial experiment in two fields (environments), one unfertilized and one fertilized. Plants were inoculated with S. rolfsii and disease was evaluated at 3-day intervals for 46 days. T. harzianum T9 and B. firmus BSR032 reduced disease incidence by 48% and 49%, respectively, whereas T. harzianum T9 + B. firmus BSR032 reduced disease incidence by 37%. The efficacy of T. harzianum T9 and B. firmus BSR032 for control of S. rolfsii was dependent on environments and genotypes. The expression of host plant resistance also depended on the environment. However, HEL246 showed consistently low disease incidence and severity index in both environments (fertilized and unfertilized). Individually, T. harzianum T9, B. firmus BSR032, or host plant resistance control stem rot caused by S. rolfsii in JA. However, no combination of these treatments provided more effective control than each alone.

A Study on Synthesis of Potassium Sulfate used Sodium Sulfate and Potassium Chloride (황산나트륨과 염화칼륨을 사용한 황산칼륨 제조 연구)

  • Kim, Nam-Il;Kim, Tae-Yeon;Chu, Yong-Sik
    • Resources Recycling
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    • v.30 no.1
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    • pp.35-43
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    • 2021
  • In this study, Na2SO4 and KCl reagents were used to synthesize K2SO4 as a basic study for recycling byproducts generated during the manufacture of steel and cement. The mole ratio of Na2SO4 to KCl, the saturation of the solution, and the stirring temperature were controlled to derive the optimal manufacturing conditions. The microstructure and crystallinity of the materials prepared were evaluated using scanning electron microscopy and X-ray diffraction analysis. Pure K2SO4 was obtained when the mole ratio of Na2SO4 to KCl was 1:6-18, the saturation of the solution was less than 160%, and the stirring temperature was 20℃, 50℃. The optimal manufacturing conditions to maximize the crystallinity and yield of K2SO4 while minimizing the energy consumption were 1:6 mole ratio of Na2SO4 to KCl, 140% saturation of the solution, and 20℃ stirring temperature.

Expression of Codon Optimized β2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of β-Agonist Residues in Pork

  • Liu, Yuan;Wang, Jian;Liu, Yang;Yang, Liting;Zhu, Xuran;Wang, Wei;Zhang, Jiaxiao;Wei, Dong
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1470-1477
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    • 2019
  • ${\beta}_2$-adrenergic receptor (${\beta}_2-AR$) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of ${\beta}$-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and $59.57{\mu}g/l$, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was $3.2{\mu}g/l$ and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified ${\beta}_2-AR$ would have great application potential in detection of ${\beta}$-agonist residues.

Improvement in Tensile Strength, Scratch Resistance and Tribological Performance of Cu-based Bimetals by Surface Modification Technology (표면개질 기술에 의한 Cu 기반 바이메탈의 인장강도, 스크래치 저항성 및 트라이볼로지 성능 향상)

  • Karimbaev, R.;Amanov, A.
    • Tribology and Lubricants
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    • v.37 no.3
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    • pp.83-90
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    • 2021
  • In this study, an ultrasonic nanocrystal surface modification (UNSM) was used to improve the mechanical properties, scratch resistance and tribological performance of Cu-based bimetals, which are usually used to manufacture sliding bearings and bushings for internal combustion engines (ICEs). Two different Cu-based bimetals, namely CuPb10Sn10 and CuSn10Bi7, were sintered onto a low carbon steel substrate. The mechanical properties and dry tribological performance using a tensile tester and micro-tribo tester were evaluated, respectively. The scratch resistance was assessed using a micro-scratch tester at an incremental load. The tensile test results showed that the yield strength (YS) and ultimate tensile strength (UTS) of both Cu-based bimetals increased after UNSM. Furthermore, the scratch and tribological tests results revealed that the scratch resistance and tribological performance of both Cu-based bimetals were improved by the application of UNSM. These improvements were mainly attributed to the eliminated pores, increased hardness and reduced roughness after UNSM. CuSn10Bi7 demonstrated better mechanical properties, scratch resistance and tribological performance than CuPb10Sn10. It was found that the presence of Bi in CuSn10Bi7 formed a Cu11Bi7 intermetallic phase, which is harder than Cu3Sn. Hence, CuSn10Bi7 demonstrated higher strength and wear resistance than CuPb10Sn10. In addition, a CuSn10Bi7 formed both SnO2 and Bi2O3 that prevented adhesion and improved the tribological performance. It can be expected that under dry tribological conditions, ICEs can utilize UNSM bearings and bushings made of CuSn10Bi7 instead of CuPb10Sn10 under oil-lubricated conditions.

A Risk Assessment Method using Disaster Influence Factors on Construction Project (건설 프로젝트의 재해영향요인 기반 위험성 평가방법)

  • Yu, Yong-Sin;Choi, Jae-Wook;Kim, Tae-Wan;Lee, Chansik
    • Journal of the Architectural Institute of Korea Planning & Design
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    • v.35 no.6
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    • pp.3-12
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    • 2019
  • Current risk assessment methods typically determine accident risks embedded in construction projects by combining severity and frequency; however, they do not reflect the characteristics of construction projects. To solve the problem, this study aims to develop a novel risk assessment method that combines severity, frequency, and disaster influence factors (i.e., weather conditions and worker's characteristics) for assessing risks of activities occurring on a construction site actually. In this study, a severity was estimated by death against victims, and a frequency was estimated by the victim rate. The frequency was then converted to probability taking disaster influence factors into account. Thus, instead of considering severity and frequency for assessing the original risks (RO), the proposed method uses severity and probability to yield adjusted risks (RA) for each activity. A case study was conducted to determine if the proposed method works as intended in a real setting. The results show that RA is more sensitive to disaster influence factors than RO and, therefore, is able to assess the actual risks reflecting the working environment and conditions of a construction site. This study contributes to risk management of construction projects by offering a risk assessment method that measures a possibility of potential disasters from the probabilistic perspective. This method would help project managers assess accident risks in a more systematic and quantitative manner.

Structural Stability of High-temperature Butterfly Valve Using Interaction Analysis

  • Lee, Moon-Hee;Son, In-Soo
    • Journal of the Korean Society of Industry Convergence
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    • v.23 no.6_1
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    • pp.881-888
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    • 2020
  • A butterfly valve is a valve that adjusts flow rate by rotating a disc for about 90° with respect to the axis that is perpendicular to the flow path from the center of its body. This valve can be manufactured for low-temperature, high-temperature and high-pressure conditions because there are few restrictions on the used materials. However, the development of valves that can be used in a 600℃ environment is subject to many constraints. In this study, the butterfly valve's stability was evaluated by a fluid-structured interaction analysis, thermal-structure interaction analysis, and seismic analysis for the development of valves that can be used in high-temperature environments. When the reverse-pressure was applied to the valve in the structural analysis, the stress was low in the body and seat compared to the normal pressure. Compared with the allowable strength of the material for the parts of the valve system, the minimum safety factor was approximately 1.4, so the valve was stable. As a result of applying the design pressures of 0.5 MPa and 600℃ under the load conditions in the thermal-structural analysis, the safety factor in the valve body was about 3.4 when the normal pressure was applied and about 2.7 when the reverse pressure was applied. The stability of the fluid-structure interaction analysis was determined to be stable compared to the 600℃ yield strength of the material, and about 2.2 for the 40° open-angle disc for the valve body. In seismic analysis, the maximum value of the valve's stress value was about 9% to 11% when the seismic load was applied compared to the general structural analysis. Based on the results of this study, the structural stability and design feasibility of high-temperature valves that can be used in cogeneration plants and other power plants are presented.

Optimization of Medium Composition for Biomass Production of Lactobacillus plantarum 200655 Using Response Surface Methodology

  • Choi, Ga-Hyun;Lee, Na-Kyoung;Paik, Hyun-Dong
    • Journal of Microbiology and Biotechnology
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    • v.31 no.5
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    • pp.717-725
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    • 2021
  • This study aimed to optimize medium composition and culture conditions for enhancing the biomass of Lactobacillus plantarum 200655 using statistical methods. The one-factor-at-a-time (OFAT) method was used to screen the six carbon sources (glucose, sucrose, maltose, fructose, lactose, and galactose) and six nitrogen sources (peptone, tryptone, soytone, yeast extract, beef extract, and malt extract). Based on the OFAT results, six factors were selected for the Plackett-Burman design (PBD) to evaluate whether the variables had significant effects on the biomass. Maltose, yeast extract, and soytone were assessed as critical factors and therefore applied to response surface methodology (RSM). The optimal medium composition by RSM was composed of 31.29 g/l maltose, 30.27 g/l yeast extract, 39.43 g/l soytone, 5 g/l sodium acetate, 2 g/l K2HPO4, 1 g/l Tween 80, 0.1 g/l MgSO4·7H2O, and 0.05 g/l MnSO4·H2O, and the maximum biomass was predicted to be 3.951 g/l. Under the optimized medium, the biomass of L. plantarum 200655 was 3.845 g/l, which was similar to the predicted value and 1.58-fold higher than that of the unoptimized medium (2.429 g/l). Furthermore, the biomass increased to 4.505 g/l under optimized cultivation conditions. For lab-scale bioreactor validation, batch fermentation was conducted with a 5-L bioreactor containing 3.5 L of optimized medium. As a result, the highest yield of biomass (5.866 g/l) was obtained after 18 h of incubation at 30℃, pH 6.5, and 200 rpm. In conclusion, mass production by L. plantarum 200655 could be enhanced to obtain higher yields than that in MRS medium