To determine anti-lodging effect of paclobutrazol and its influence on yield of rice, paclobutrazol was applied at 12 and 18g ai/10a to cv. Nakdongbyeo and cv. Seomjinbyeo 20, 15, and 10 days before heading under 12 and 20kg/10a nitrogen conditions. 1. Paclobutrazol reduced internode length of rice, especially internode N3. Higher suppression of stem of both cultivars and reduction of ear length in cv. Nakdongbyeo were observed when paclohutrazol was applied at 18g ai/10a 15 and 20 days before heading. 2. Paclobutrazol increased culm cross-section area, culm diameter, linear density and stem flexure resistance which affected anti-lodging efficacy. Although actual lodging was not occurred in field, less stem inclination was observed at paclobutrazol treated plots. 3. There were significant correlation between stem flexure resistance and culm cross-section area, culm diameter and linear density, and negative correlation between straw inclination angle and culm cross-section area and culm diameter. 4. Slight yield reduction was observed when paclobutrazol was applied at 18g ai/10a 20 days before heading. However, when paclobutrazol was applied at 12g ai/10a 10 and 15 days before heading, rice yield was not decreased compared to untreated control.
Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized. The phytase had an optimum temperature at $25^{\circ}C$, which is the lowest among all the known phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and temperature, MiPhyA showed the maximum activity level ($2,384.6{\pm}90.4{\mu}mol{\cdot}min^{-1}{\cdot}mg^{-1}$, suggesting that the enzyme possesses a higher activity level over many known phytases at low temperatures. The phytate-degrading efficacy was tested on three common feed materials (soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could yield at least $3{\times}$ more inorganic phosphate than the two reference phytases. When using the same weight of protein, MiPhyA could yield at least $5{\times}$ more inorganic phosphate than the other two. Since it could degrade phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, MiPhyA might be a promising candidate as a feed additive enzyme.
Nam, Cheol Hwan;Kim, Ki Soo;Park, Man Ho;Kim, Won Ho;Ji, Hee Jung;Choi, Ki Chun;Sun, Sang Soo
Journal of The Korean Society of Grassland and Forage Science
/
v.38
no.2
/
pp.91-98
/
2018
This study was conducted in 2014 in the Gangjin area of Jeonnam province for the purpose of confirming the potential of Kenaf as an alternative feed crop of rice. The weather and soil conditions were favorable and there was no adverse effect on the growth of Kenaf. The amount of seeding was given at 10 kg, 15 kg and 20 kg. As the seeding volume increased, plant height and dry matter yield increased, and stem diameter and number of nodes decreased. The highest growth rate was observed between 75 and 90 days from the date of planting(p<0.05). As the growth progressed, stem rate increased and leaf rate decreased(p<0.05). Increased amount of seeding and growth resulted in decreased CP and TDN and increased ADF and NDF, which resulted in the decreased economic value of Kenaf as a feed crop. The result of increasing the use of organic fertilizer to 20 kg and 30 kg was similar to increasing the amount of seeding. As organic fertilizer usage increased, dry matter yield and growth rate improved(p<0.05).
At the initial stage of the underground reservoir design one should thoroughly consider surface and subsurface hydrology, hydrogeologic characteristics of aquifer system, and the function of cut - off wall because it is linked to the effective management. In this study, three dimensional finite difference model was applied to analyse the function of Ian underground reservoir at Kyungbuk Province. The steady and unsteady state conditions after construction of the underground dam were simulated through the model, and from these results the groundwater budget and the safe yield were determined. The model simulation indicates the infiltration of irrigation water to be one of the major factors of seasonal fluctuation of groundwater level. The recharge rates of irrigation water were estimated as 4.3mm/d during May and June, and 1.7mm/d during July and Agust. Groundwater recharge from the watershed area estimated to about $0.04m^3/s$, almost consistent through the year. In 1984, groundwater discharge through the transverse section of the dam was $0.002m^3/s$ and the optimum yield for two momths(July and Aguest)was $254000m^3$, however, the discharge became $0.013m^3/s$ in1993, implying the failure of cut -off function. without appropaiate of the cut - off wall, optiumum yield during the irrigaton period would be $93, 000m^3$.
This study was conducted to identify the appropriate nitrogen fertilizer application rate for improving rice quality in tidal reclaimed area, at the Gyehwado substation of the Honam Agricultural Research Institute during 2002-2(103. The experimental fields contained 0.1% (low salinity) and 0.3-0.4% (medium salinity) Nacl in soil solution. Plant height at panicle formation stage was tall ay heavy nitrogen level and the effect of heavy nitrogen was higher in low than in high soil salinity condition. Heading date was not affected by applied nitrogen levels from 8 to 16 kg/10a in low soil salinity condition but it was one day later in 24 kg/10a nitrogen level when compared with the standard nitrogen level,20 kg/10a. In middle soil salinity condition, the heading date was one day earlier in 8 to 16 kg/10a and similar in 24 kg/10a, when compared with 20 kg/10a nitrogen level. And also it was four days later in middle than in low soil salinity condition. In low soil salinity condition, grain number $\textrm{m}^2$ increased but ripened grain ratio decreased as the nitrogen application increased and finally, milled rice yield was not different among heavy nitrogen application levels compared with 12 kg/10a. Head rice ratio was high and protein content was low in 12 kg/10a or lower nitrogen level. In middle soil salinity condition, grain number $\textrm{m}^2$ increased and ripened grain ratio was not affected as the nitrogen application increased. And finally, milled rice yield increased with increasing nitrogen application levels, Head rice ratio was high and protein content was not affected by nitrogen application levels. Therefore, on the basis of milled rice yield and rice grain quality inreclaimed land, the appropriate nitrogen application level would be 12 kg/10a in low soil salinity condition and 20 kg/10a in middle soil salinity condition.
Journal of The Korean Society of Grassland and Forage Science
/
v.11
no.4
/
pp.258-263
/
1991
There are ten NLCF's recommended sorghum-sudangrass hybrids(Sorghum bicolor (L.) Moench), but they have not been evaluated under same environmental conditions. Therefore, a field experiment was conducted to campare forage yield and quality of the NLCF's recommended cultivars of sorghum-sudangrass hybrids at the experimental forage field, College of Agriculture, SNU, Suweon over two years. Among the ten cultivars used, G83F and NC+855 were classified as early maturing cultivars. Pioneer 988 and Sordan 79 were more resistant to leaf blight than the other hybrids. Jumbo, Sordan 79 and SX-17 were susceptible to lodging. No significant differences in dry matter yield were found among the cultivars used, but the dry matter yields of Pioneer 988, TE-Haygrazer and G83F were slightly higher than the other hybrids in 1989 and those of NC+855 and Pioneer 988 were higher in 1990. There were no marked cultivar differences in CP, ADF, NDF and RFV at the first, second and third cuts in 1990, but the ADF, NDF and RFV of sorghum-sudangrass hybrids at the third cut showed more higher than those of the first and second cut forage. In conclusion, there are no forage yield differences among the ten NLCF's recommended sorghum-sudangrass hybrids.
These experiments were carried out to determine the optimal culture conditions for nine strains of collected Hatakeshimeji, Lyophyllum decastes (Fr.:Fr.) Sing. SPA 202 and SPA 205 strains were selected because mycelium grew fast and showed fine density. All strains showed fast mycelial growth and mycelial density on BC(Burke compost) media for 20 days of incubation. The optimal sawdust species for the mycelial growth were the fermented sawdusts of Quercus aliena and Populus deltoides. Spawn running period on the fermented sawdust substrate required 50 days at 20 to $25^{\circ}C$ and additional 7 days after soil casing. Cultivation period and temperature forprimordia formation and fruitbody development appeared from 10 to 11 days and from 7 to 8 days at 17 to $18^{\circ}C$ respectively. The length of pilei and stipes of SPA 202 harvested in optimal stage showed 60mm and 67mm, respectively. Yield of SPA 202 strain grown on fermented sawdust substrate was 130g per 1,100ml in bottle cultivation. The length of pilei and stipes of SPA 205harvested in optimal stage showed 51mm and 81mm, respectively. Yield of SPA 205 strain grown on fermented sawdust substrate was 129g per 1,100 ml in bottle cultivation. SPA 202 strain and SPA 205 strain in artificial bottle cultivation of Lyophyllum decastes used in fermented sawdust substrate were selected as themost appropriate strain in yield.
Park, Kyung-Bae;Awh, Ok-Doo;Kim, Jae-Rok;Lim, Sang-Moo;Hong, Seong-Woon
The Korean Journal of Nuclear Medicine
/
v.23
no.1
/
pp.71-83
/
1989
For the development of $^{99m}Tc-labelled$ antimony sulfide colloid and hydroxyethyl starch, various experiments such as preparation of colloid, control of the distribution of particle size, establishment of labelling conditions, determination of labelling yield and radiochemical purity, examination of stability, and organ imagings of rabbits etc. were carried out. 1) Antimony sulfide colloid was readily prepared by the reaction of aqueous solution of antimony potassium tartrate with hydrogen sulfide generated by treating ferrous sulfide with dilute sulfuric acid. The colloid could be stabilized by adding small amount of polyvinylpyrrolidone. 2) Electron microscopy analysis exhibited the distribution of colloid size in the range of $1\sim15nm$ with a major portion of 9 m. The colloid solution was sterilized by membrane filtration $(0.2{\mu}m)$ and then stored at $4^{\circ}C$. This sterilized colloid was so stable that it was usable at least for one year. 3) The antimony sulfide colloid was labelled by adding sodium $pertechnetate-^{99m}Tc$ solution to the reaction vial, followed by adding hydrochloric acid and then boiled for 30 min. The optimal pH of the reaction mixture was found to be in the range of $1.3\sim1.4$. Instant thin layer chromatography (ITLC) analysis showed high labelling yield of above 99.5%. This labelled colloid maintained high radio-chemical purity of above 99% until 10 hours after labelling. 4) Animal studies showed high uptake of $^{99m}Tc-Sb_2S_3$ colloid at lymph vessels and nodes indicating a suitable agent for lymphoscintigraphy. Satisfactory results were also abtained in other clinical studies. 5) Hydroxyethyl starch (HES $0.6\sim1.0%$) was labelled with $Na^{99m}TcO_4$ in the presence of $SnCl_2$ with high labelling yield of above 99.5%. The optimal pH of the reaction mixture was in the range of $1.8\sim2.0$. $^{99m}Tc-HES$ maintained high radiochemical purity of above 99% until 10 hours after labelling. 6) Animal studies showed that $^{99m}Tc-HES$ migrated more rapidly from the injection sites into the lymph vessels than $^{99m}Tc-Sb_2S_3$ colloid while less amount of the former was uptaken at lymph nodes than that of the latter. Similar phenomenon was also observed in other clinical studies. As a result, $^{99m}Tc-Sb_2S_3$ colloid was found to be more effective lymphoscintigraphic agent than $^{99m}Tc-HES$.
BACKGROUND: To evaluate the impact of rapid temperature change at spring and the early summer seasons in climate change, we have investigated the physiological response and yield of onion in a greenhouse with thermostat control system. METHODS AND RESULTS: Seedlings of onion(cv. Sunshine) were planted on October 30, 2012 and harvested on May 30, 2013. The used treatments(March-April-May) for a rapid temperature change were T0(control): $6.0-10.4-17.2^{\circ}C$, T1: $6.0-5.4(-5)-17.2^{\circ}C$, T2: $6.0-10.4-22.2(+5)^{\circ}C$ and T3: $6.0-5.4(-5)-22.2(+5)^{\circ}C$. Total yields of bulb within the temperature change as high temperature treatment T2 and control treatment T0 were increased significantly(p<0.05), as compared to the low temperature treatment T1. Low temperature conditions significantly (p<0.05) reduced plant height, SPAD reading, crude protein and fiber etc., as compared to the TO and T2. CONCLUSION: The rapid temperature changes were highly affected by low temperature than high temperature. These results suggest that rapid climate change of future could need systematic standard model for physiological characteristics and yields of onion.
The task of plastic theory is twofold: first, to set up relationships between stress and strain that adequately describe the observed plastic deformation of metals, and second, to develop techniques for using these relationships in studying of the mechanics of metal forming processes, and the anlaysis and design of structures. One of the major problems in the theory of plasticity is to describe the behavior of work-hardening materials in the plastic range for complex loading histories. This can be achieved by formulating constitutive laws either in the integral or differential forms. To adequately predict the response of steel members during cyclic loading, the hardening rule must account for the features of cyclic stress-strain behavior. Neithe of the basic isotropic and kinematic hardening rules is suitable for describing cyclic streess-strain behavior, although a kinematic hardening rule describes the nearly linear portions of the stabilized hystersis loops. There is also a limited expansion of the yield surface as predicted by the isotropic hardening rule. Strong ground motions or wind gusts affect the complex and nonproportional loading histories in the inelastic behavior of structues rather than the proportional loading. Nonproportional loading is defined as externally applied forces on the structure, with variable ratios during the entire loading history. This also includes the rate of time-dependency of the loads. For nonproportional loading histories, unloading may take place along a chord instead of the radius of the load surface. In such cases, the shape of the stress-strain curve has to be determined experimentally for all non-radial loading conditions. The plasticity models including two surface models ae surveyed based on a yield surface and a bound surface that represent a state of maximum stress. This paper is concerned with the improvement of a plasticity models of the two-surface type for structural steel. This is follwed by an overview of plasticity models on structural steel. Finally the need for further research is identified.
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