• Title/Summary/Keyword: yeast solution

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Washing for Debittering of Brewers Yeast Slurry (맥주효모 슬러리의 쓴맛을 제거하기 위한 세척)

  • Kim, Jae-Sik
    • Korean Journal of Food Science and Technology
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    • v.33 no.2
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    • pp.205-208
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    • 2001
  • The bitterness of brewers yeast slurry decreased by washing with mild caustic soda solution followed by washing with 0.85% (w/v) NaCl solution The higher concentration of caustic soda was, the lower the bitterness unit(BU) of washed yeast slurry was. The lethal rate of yeast cells increased. When the concentration of caustic soda solution increased from 0.05%(w/v) to 0.25%(w/v), the BU of brewers yeast slurry was decreased from 45 to 3, but yeast cells viability decreased from 93% to 0%. The optimum washing conditions of brewers yeast slurry were as follows: the concentration of caustic soda solution was $0.07{\sim}0.1%$(w/v) and the contact time of brewers yeast slurry with caustic soda was $10{\sim}20$ minutes. The similar washing effect was obtained when the brewers yeast slurry was washed with 20%(v/v) ethanol solution.

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Eco-friendly Indigo Dyeing using Baker's Yeast: Reducing Power according to Alkaline Solution Type (효모를 사용한 친환경 인디고 염색: 알칼리수용액 종류에 따른 환원력)

  • Son, Kyunghee;Shin, Younsook
    • Textile Coloration and Finishing
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    • v.31 no.4
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    • pp.249-257
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    • 2019
  • Baker's yeast(Saccharomyces cerevisiae) was used as a biocatalyst for eco-friendly indigo dyeing and the reducing power of yeast according to the alkaline solution type was compared. NaOH solution, lye, and buffer solution were used as alkaline solutions. The reducing power(K/S value, oxidation/reduction potential(ORP), pH) was monitored according to the elapsed time including the initiation of reduction, peak reduction, and the end of reduction. In all alkaline solutions, it was confirmed that yeast can be used reducing agent in indigo reduction dyeing. The pH stability and reducing power of buffer solution was better than that of NaOH alone. Although, pH and ORP stability of the reduction bath in lye were better than that of buffer solution, K/S value in buffer solution was higher compared to lye. The reducing power was different depending on the starting pH of the dye bath, and it was better when starting at pH 10.70 than at pH 11.30. Fastnesses to washing, rubbing, and light were relatively good with above rating 4. There was no significant difference in colorfastness depending on the type of alkaline solutions.

Reduction of m-Bromonitrobenzene and Nitrosobenzene with Bakers' Yeast (Bakers' Yeast를 이용한 m-Bromonitrobenzene 및 Nitrosobenzene의 환원반응)

  • Kim, Kyungsoon;Baik, Woonphil;Oh, Sunghwan
    • Journal of the Korean Chemical Society
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    • v.39 no.10
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    • pp.812-817
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    • 1995
  • Rapid and selective reduction of aromatic nitro compounds is of important for the preparation of amino derivertives in organic synthesis, particularly when a molecule has other reducible substituents. While Bakers' Yeast has been used for the enantioselective reduction of carbonyl compounds, little attention has been paid to the reduction of aromatic nitro compounds with Bakers' Yeast. Nitro group of m-bromonitrobenzene was selectively and rapidly reduced to corresponding amino derivative in good yield by Bakers' Yeast in basic solution. Furthermore, nitrosobenzene was rapidly reduced to aniline in good yield by Bakers' Yeast under neutral condition. In this paper, we wish to report a rapid and simple reduction of m-bromonitrobenzene and nitrosobenzene to the corresponding amino derivatives using Bakers' Yeast. And the effects of various agents, temperature and pH on the reduction will be discussed.

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Permeation Characteristics of the Tubular Membrane with Continuous Air Cleaning System (연속식 공기세정 관형막 투과특성)

  • Park, Mi Ja;Chung, Kun Yong
    • Membrane Journal
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    • v.23 no.2
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    • pp.185-188
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    • 2013
  • This study was carried out for microfiltration tubular membrane module equipped with self-designed air injection nozzle in order to determine the permeate flux due to the effect of membrane fouling reduction. The 0.1 wt% yeast particle solution was used as a feed solution and permeation tests were performed for the cases with and without air injection. Permeation fluxes were measured and analyzed to examine the effect of membrane fouling reduction. While the permeation flux without air injection decreased continuously, that with air injection was improved more than 30 percent than that of no air injection case.

Characterization of Vitamins in Yeast Extract using Gel Filtration, Ion Exchange Chromatography and HPLC (젤 여과, 이온 크로마토그래피와 HPLC에 의한 효모 엑기스내의 비타민의 분석연구)

  • 최인호;홍억기;강환구;김인호
    • KSBB Journal
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    • v.15 no.1
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    • pp.76-79
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    • 2000
  • Complex, ill-defined mixtures of natural origin are often used as nutrients in the production of biological products through microbial fermentation. Product yields are affected by variation in these natural products. Yeast extract is a typical example of these natural products. Since it is a mixture of amino acids, peptides and nucleic acids, its composition is not well characterized. In this study, we investigated the properties of thiamine hydrochloride, riboflavin and pyridoxine hydrochlride in yeast extract by using a gel filtration chromatography, ion exchange chromatography and high performance liquid chromatography. Yeast extract solution was fractionated by gel filtration chromatography and ion exchange chromatography, and then, each fraction was analyzed by using a high performance liquid chromatography.

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Yeast Loading BOD Biosensor (효모균을 이용한 BOD Biosensor)

  • Kim, Mal-Nam
    • The Korean Journal of Mycology
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    • v.23 no.4 s.75
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    • pp.354-358
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    • 1995
  • A yeast loading biochemical oxygen demand (BOD) sensor was designed and constructed to quickly measure the concentration of biologically assimilable organic substances dissolved in water as BOD values to feed back to the waste water treating processes. The sensitivity of the BOD sensor reached maximum at around pH 7.0 and $30^{\circ}C$ where yeast showed the highest assimilation activity. Biomass also affected the sensor output, and biomass of $ 0.14\;mg/cm^2$ on the dialysis membrane appeared to be the optimum cell mass level. The sensitivity of the sensor depended on the kinds of pollutants and increased considerably when the yeast was preincubated in the solution of respective pollutants before loading on the sensor.

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Physicochemical and Functional Properties of Yeast-Fermented Cabbage

  • Ahhyeon Chun;So Jeong Paik;Jongbeom Park;Ryeongeun Kim;Sujeong Park;Sung Keun Jung;Soo Rin Kim
    • Journal of Microbiology and Biotechnology
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    • v.33 no.10
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    • pp.1329-1336
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    • 2023
  • Microbial fermentation is often used to improve the functionality of plant-based food materials. Herein, we investigated changes in the physicochemical and functional properties of cabbage during yeast fermentation to develop new products using fermented cabbage. Among the 8 types of food-grade yeast, both Saccharomyces cerevisiae and Saccharomyces boulardii fermented 10% cabbage powder solution (w/w) the most effectively, leaving no soluble sugars after 12 h of fermentation. In addition, the yeast fermentation of cabbage resulted in functionally positive outcomes in terms of sulforaphane content, antioxidant properties, and anti-inflammatory activity. Specifically, the yeast-fermented cabbages contained about 500% more sulforaphane. The soluble fraction (5 ㎍/ml) of yeast-fermented cabbage had no cytotoxicity in murine RAW 264.7 cells, and the radical-scavenging capacity was equivalent to 1 ㎍/ml of ascorbic acid. Moreover, cabbage fermented with S. boulardii significantly suppressed both lipopolysaccharides (LPS)-induced nitric oxide production and LPS-induced reactive oxygen species production in RAW 264.7 cells, suggesting a potential anti-inflammatory effect. These results support the idea that yeast fermentation is promising for developing functionally improved cabbage products.

Cell Cycle Regulation in the Budding Yeast

  • Nguyen, Cuong;Yoon, Chang-No;Han, Seung-Kee
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2005.09a
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    • pp.278-283
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    • 2005
  • Cell cycle is regulated cooperatively by several genes. The dynamic regulatory mechanism of protein interaction network of cell cycle will be presented taking the budding yeast as a sample system. Based on the mathematical model developed by Chen et at. (MBC, 11,369), at first, the dynamic role of the feedback loops is investigated. Secondly, using a bifurcation diagram, dynamic analysis of the cell cycle regulation is illustrated. The bifurcation diagram is a kind of ‘dynamic road map’ with stable and unstable solutions. On the map, a stable solution denotes a ‘road’ attracting the state and an unstable solution ‘a repelling road’ The ‘START’ transition, the initiation of the cell cycle, occurs at the point where the dynamic road changes from a fixed point to an oscillatory solution. The 'FINISH' transition, the completion of a cell cycle, is returning back to the initial state. The bifurcation analysis for the mutants could be used uncovering the role of proteins in the cell cycle regulation network.

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Production Condition and Utilities of Extracellular Biopolymer from Bacillus licheniformis (Bacillus licheniformis가 생산하는 Extracellular Biopolymer의 생산조건 및 이용특성)

  • 진효상;이완옥
    • Korean Journal of Environmental Biology
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    • v.18 no.1
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    • pp.199-203
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    • 2000
  • A bacterium that produce biopolymer was isolated from Gochujang, one of Korean traditional fermented foods, and identified as Bacillus licheniformis. The production of biopolymer was highest and 34mg/250ml, when the baterium was cultivated in condition of sucrose 6.0%, Yeast extract 0.1%, peptone 0.l%, NaCl 3.0%, and pH 6.0. The 1% solution of this biopolymer was able to form a translucent and glossy film. And the solution of biopolymer was found to precipitate Kaolin solution and also stabilize the suspension of lactic acid bacteria within the test range of 0.25-1.00%. [Bacillus licheniformis, Biopolymer].

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Studies on the Fermentative Utilization of Cellulosic Wastes (part III) Production of Yeast from the Hydrolyzate of Rice straw, Rice hull and Corn Starch Pulp. (폐섬유자원의 발효공학적 이용에 관한 연구 (제3보) 볏짚, 왕겨및 전분박 당화액을 이용한 효모배양)

  • 성낙계;심기환;이천수
    • Microbiology and Biotechnology Letters
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    • v.4 no.4
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    • pp.152-158
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    • 1976
  • Cultivation condition of yeast on the utilization of fermentable substrate from the cellulosic wastes such as rice hull, rice straw and corn starch cake was investigated. The results obtained were summarized as follows;1. Corn starch cake was respectively added to rice hull and rice straw in order to increase sugar concentration in the hydrolyzate, and then hydrolyzed. As the result, concentration of sugar in hydrolyzed solution of rice hull was 9.12%, in that of rice straw was 7.98%. 2. It was found that calcium carbonate as a neutralizer was the most effective to prepare the culture broth of yeast. 3. An optimal growth of Hansenula subpelliculosa GFY-2 was observed in the medium prepared by adding 0.3% of ammonium sulfate, 0.4% of potassium phosphate dibasic, 0.02% of magnesium sulfate, sodium chloride and calcium chloride to hydrolyaed sugar solution, respectively. 4. Hansenula subpellicuiosa GFY-2 cultured in the substrate solution which of rice hull and rice straw added to corn starch cake was assimilated more than 90% of sugar in the hydrolyzate within 48 hours. The yeast cells yielded in rice hull was 46.5%, and that of rice straw 45.4% to utilized sugars.

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