• Journal of the Korean BIBLIA Society for library and Information Science
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• v.28 no.4
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• pp.461-480
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• 2017

The purpose of this research is to explore young adults (YA)' perceptions, use, and needs of public libraries, as well as to find out how to make effective YA sections within public libraries. Two public libraries with separate YA sections were selected and a total of 126 YA users participated in the survey. The survey showed 86.5% of participants used the YA section and the main reasons for use were reading for leisure, study area use, and seeking information for school assignments. 64.8% of participants agreed that separate YA sections are needed at public libraries. The areas most in need of improvement were expansion of seats of YA section, lack of digital devices, and expansion of group study and meeting spaces. This research highlights for space composition policy as YA's needs, creating a balanced environment for physical facilities, and YA oriented service. As well, a need for improved opening hours, and expansion of multi-use spaces for seats of the YA section.

• Journal of Life Science
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• v.11 no.1
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• pp.42-46
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• 2001

Subtilisin YaB, produced by alkalophilic Bacillus strain YaB, is an extracellular alkaline serine protease having 55% homology to subtilisin BPN'. It is synthesized as a 378-amino acid preproenzyme and secreted into the culture medium as a 265-amino acid mature protease. To examine the role of pro-sequence for the secretion of subtilisin YaB, we have studied the expression, in Bacillus subtilis, of a mutant preprosubtilisin YaB in which active site Ser214 is substituted with Cys. The use of a six protease-deficient strain, WB600, was required for its efficient production. The prosubtilisin YaB, thus produced, was indeed secreted into the culture medium and was processed to its mature form upon treatment with exogenously added active subtilisin YaB. From these results, we have concluded that the processing of pro-sequence is not essential for the secretion of the enzyme.

• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.275-281
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• 1995

From the plasmid pYA300 carring a CMCase of Rhizobium fredii USDA193 plasmid was subcloned into pBluescript II KS(+)/pBluescript II SK(+) vectors and designated pYA500 and pYA600, respectively. Escherchia coli cells transformed with pYA500 porduced the CMCase more than with pYA600. The orientation of the cloned fragment in pBluescript vector had the effect on gene expression in E. coli background. When the 1.7 kb CMCase gene fragment of R. fredii USDA193 was hybridized to EcoRI-digested total DNA from R. meliloti and R. fredii USDA 191 the unique bands hybridized respectively, indicating that some genetic diversity exists in the EcoRI restriction enzyme site for CMCase gene in Rhizobium strains. The optimum pH of enzyme activity was 7 and the optimum temperature of that was nearly 37$\circ$C. The cellulase-minus derivatives of pYA500 were constructed by Tn5 insertional mutation. Among 6000 transconjugants, two mutant plasmids (designated pYA500::Tn5a and pYA500::Tn5b) were detected from the cellulase- negative transconjugants. The product of CMCase gene was analyzed by one dimensional SDS- PAGE of the cell extracts. About 45 kDa protein was considered to be a product of CMCase gene.

• The Optical Journal
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• s.96
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• pp.37-44
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• 2005

• Molecules and Cells
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• v.37 no.2
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• pp.188-188
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• 2014

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.3
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• pp.219-224
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• 1991

An acid tolerant R. meliloti "YA03" which was characterized through both acidified tube and pot soil experiment, was examined on its inoculation effect to alfalfa[Medicago sativa (L.)]cv. Vernal on hilly acid soil. It was conducted for 2 years to evaluate inoculation residual effect and there were two-fertilizer factors of nitrogen(0.8kg/10a) and lime(0,162kg/10a)as urea and $Ca(OH)_2$, respectively. The results obtained were summarized as follows : 1. In the 1st-year experiment, YA03 inoculated without nitrogen and lime application showed higher symbiotic effectiveness than check strains, YA026 and Rm2011 in nodule mass, $N_2$-fixing activity, and dry matter yield, but with N 8kg/10a application lower effectiveness than Rm2011. 2. In the 2nd-year experiment, Alfalfa dry matter yield by YA03 inoculation with N application(without lime) was 74% higher than that(276 kg/10a) in the 1st year, and the value showed 166%, 20%, and 21% increments as compared with noninoculated control(180kg/10a). YA026(399kg/10a), and Rm2011(397kg/10a), respectively. 3. In the 2nd-year experiment of N applicated plote(without lime), especially, YA03 showed higher shoot nitrogen(7.7kg N/10a/Yr.) fixed and soil rhizobial population($1{\times}10^4\;cells/g.\;soil$) than check strains. 4. On the basis of lime application, application effect of three major fertilizers with YA03 inoculation on alfalfa yield and soil rhizobial population was in the order of $N+P_2O_5+K_2O>P_2O_5+K_2O>P_2O_5+K_2O$. 5. On the whole, alfalfa yield by acid tolerant R. meliloti YA03 inoculation on hilly acid soil was enhanced with nitrogen application, and besides it appeared 44% higher with lime than without lime.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.1
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• pp.61-71
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• 2018

This study sought to interpret the Ya-Aeng (夜櫻) from the viewpoint of urban society, focusing on the occurrence of the Ya-Aeng at Changgyeongwon (昌慶苑) in the modern city of Kyungsung. When the Ya-Aeng started in the 1920s, the social aspects of Kyungsung were in a transitional period from the traditional to the modern. The social modernization of Kyungsung has had a dramatic impact on the Ya-Aeng as a part of the city culture. Using the concept of 'phantasmagoria', which was widespread in Kyungsung society and the Ya-Aeng, this study has established three implications of the Ya-Aeng. First, Kyungsung's phantasmagoria appeared in the form of crowds, spectacles, and experiences. This study suggests that such interpretation also applies to the Ya-Aeng. This means that the capitalism-controlled modern society on one hand and the Ya-Aeng on the other had the same mechanism. Therefore, the Ya-Aeng, as modern city culture, becomes a miniature version of Kyungsung and a modern commodity world in itself. Second, the fact that phantasmagoria is a major element of the landscape of the Ya-Aeng means that there is a special way of seeing. For modern subjects, the phantasmagoria of the Ya-Aeng has acted as a learning mechanism for a modern way of seeing. Third and finally, the phantasmagoria of the Ya-Aeng was an illusion to encourage the continued consumption of this culture and at the same time, forget about the capitalism-controlled urban culture. At this time, capitalism was dominated by the influence of Japanese imperialism. The significance of this study lies in that it expands the idea of the Ya-Aeng from the events inside Changgyeongwon into the urban culture, which is a projection of modern urban society. In addition, where the Ya-Aeng in the past had been regarded as a decadent and poor-quality spring celebration in comparison to the traditional spring celebration, this study proposes a new point of view for the Ya-Aeng in an urban social context.

• Microbiology and Biotechnology Letters
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• v.23 no.5
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• pp.544-549
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• 1995

The alkaline elastase is an extracellular serine protease of the alkalophilic Bacillus strain Ya-B. To increase the gene copy number and the production level of the alkaline elastase Ya-B, we designed, on the B. subtilis chromosome, a gene amplification of the 10.6 kb repeating unit containing amyE, aleE (alkaline elastase Ya-B gene) and tmrB. The aleE was inserted between amyE and tmrB, and B. subtilis APT119 strain was transformed with this amyE-aleE-tmrB-junction region fragment. As a result, we succeeded in obtaining tunicamycin-resistant (Tm$^{r}$) transformants (Tf-1, Tf-2) in which the designed gene amplification of 10.6 kb occurred in chromosome. The transformants showed high productivity of $\alpha $-amylase and alkaline elastase Ya-B. The copy number of the repeating unit (amyE-aleE-tmrB) was estimated to be 25, but plasmid vector (pUC19) was not integrated. The amplified aleE of chromosome was more stable than that of plasmid in absence of antibiotics.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.1459-1462
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• 2009

최근 Yang과 An은 기존의 RFID 인증 메커니즘들이 가지고 있는 프라이버시 침해 문제를 분석하고 보다 안전하고 효율적인 사용자의 프라이버시를 보호할 수 있는 RFID(YA-RFID) 인증 프로토콜을 제안하였다. 그들은 제안한 YA-RFID 인증 프로토콜이 공격자의 재전송 공격, 스푸핑 공격, 그리고 위치 트래킹 공격에 대해 안전하다고 주장하였다. 하지만 본 논문에서는 그들의 주장과는 달리 YA-RFID 프로토콜이 여전히 위치 트래킹 공격에 취약함을 증명하며, 더 나아가 동일한 연산 효율성을 보장하며 위치 트래킹 공격을 막을 수 있는 간단히 개선된 일회성 난수와 안전한 일방향 해쉬 함수 기반의 RFID 인증 프로토콜을 제안한다.

• Journal of Life Science
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• v.11 no.4
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• pp.385-391
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• 2001

Filamentous hemagglutinin (FHA) is considered as an essential immunogenic component for incorporation into acellular vaccines against Bordetella pertussis, the causative agent of whooping cough. Classically, antipertussis vaccination has employed an intramuscular route. An alternative approach to stimulate mucosal and systemic immune responses is oral immunization with recombinant live vaccine carrier strains of Salmonella typhimurium. An attenuated live Salmonella vaccine sgrain($\Delta$cya $\Delta$crp) expressing recombinant FHA(rFHA) was developed. Stable expressionof rFHA was achieved by the use of balanced-lethal vector-host system. which employs an asd deletion in the host chromosome to impose in obligate requirement for diaminopimelic acid. The chromosomal $\Delta$asd mutation was complemented by a plasmid vector possessing the asd$^{+}$ gene. A 3 kb DNA fragment encoding immuno dominant regionof FHA was subcloned in-frame downstream to the ATG translation initiation codon in the multicopy Asd$^{+}$ pYA3341 vector to create pYA3457. Salmonella vaccine harboring pYA3457 expressed approximately 105kDa rFHA protein. The 100% maintenance of [YA3457 in vaccine strain was confirmed by stability examinations. Additionally, a recombinant plasmid pYA3458 was constructed to overpress His(8X)-tagged rFHA in Essherichia coli. His-tagged rFHA was purified from the E. coli strain harboring pYA3458 using Ni$^{2+}$-NTA affinity purification system.>$^{2+}$-NTA affinity purification system.