• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.264-264
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• 2006

The nanoparticle-hydrogel complex as a new bone defect replacement matrix, which is composed of the nanoparticles for the sustained release of BMP and the hydrogel for filling the bone defect site and playing a role as a matrix where new bone can grow, is presented. In vivo evaluation of bone formation was characterized by soft X-ray, MT staining, and calcium assay, based on the rat calvarial critical size defect model. The effective bone regeneration was achieved by the BMP-2 loaded nanoparticles in fibrin gel, compare to bare fibrin gel, the nanoparticle-fibrin gel complex without BMP-2, or the BMP-2 in fibrin gel, in terms of the new bone area and the gray level in X-ray, the bone marrow are, and the calcium content in the initial defect site. These findings suggest that the BMP-2 loaded nanoparticle-fibrin gel complex can a promising candidate for a new bone defect replacement matrix.

• Journal of Pharmacopuncture
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• v.13 no.3
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• pp.63-71
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• 2010

Objectives: Gallic acid (GA) is the major component of tannin which could be easily founded in various natural materials such as green tea, red tea, grape juice, and Corni Fructus. The purpose of this study is to investigate the effect of Gallic acid (GA) on production of interleukin (IL) in mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS). Methods: Productions of interleukins were measured by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. Firstly, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, it was incubated with the antibody-conjugated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System and concentration of interleukin was determined. Results: The results of the experiment are as follows. 1. GA significantly inhibited the production of IL-3, IL-10, IL-12p40, and IL-17 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). 2. GA significantly inhibited the production of IL-6 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). 3. GA diminished the production of some cytokine such as IL-4, IL-5, and IL-13 in LPS-induced mouse macrophage RAW 264.7 cells. 4. GA did not show the inhibitory effect on the production of IL-$1{\alpha}$ and IL-9 in LPS-induced mouse macrophage RAW 264.7 cells. Conclusions: These results suggest that GA has anti-inflammatory activity related with its inhibitory effects on the production of interleukins such as IL-3, IL-10, IL-12p40, IL-17, and IL-6 in LPS-induced macrophages.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.11
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• pp.2503-2510
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• 2012

This paper proposes a parallel operation intra predictor for H.264/AVC decoder. In previous intra predictor design, common operation units were designed for 17 prediction modes in order to compute more effectively. However, it was designed by analyzing the equation applied to one pixel. So, there are four operation units for computing 16 pixels in a $4{\times}4$ block and they need four cycles. In this paper, the proposed intra predictor contains T3(Three Type Transform) operation unit for parallel operation. It divides 17 modes into 3 types to calculate 16 pixels of a $4{\times}4$ block in only one cycle and needs 16 cycles minimum in 16x16 block. As the result of the experiment, in terms of processing cycle, the performance of proposed intra predictor is 58.95% higher than the previous one.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.4
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• pp.586-591
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• 2010

Chemokine and Growth Factor are major mediumtors of immuno-inflammatory pathway. The purpose of this study is to investigate whether productions of Chemokine and Growth Factor in lipopolysaccharide (LPS)-induced mouse macrophage RAW 264.7 cells are modulated by Gallic acid (GA), which is easily founded in tannin-containing natural materials such as red wine, green tea, grape juice, and Corni Fructus. Productions of Chemokine and Growth Factor were analyzed by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. At first, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, the antibody-conjugated beads were added and incubated for 30 minutes. After incubation, detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Based on fluorescence intensity, concentrations of Chemokine and Growth Factor were determined. The results of the experiment are as follows. GA significantly inhibited the production of interferon-inducible protein (IP)-10, keratinocyte-derived chemokine(KC), and vascular endothelial growth factor (VEGF) in LPS-induced RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). GA significantly inhibited the production of monocyte chemoattractant protein-1(MCP-1) and macrophage-colony stimulating factor(M-CSF) in LPS-induced RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). GA diminished the production of granulocyte macrophage-colony stimulating factor (GM-CSF) in LPS-induced RAW 264.7 cells. But GA did not show the inhibitory effect on the production of leukemia inhibitory factor (LIP) and macrophage inflammatory protein (MIP)-2 in LPS-induced RAW 264.7 cells. These results suggest that GA has the immuno-modulating activity related with its inhibitory effects on the production of IP-10, KC, MCP-1, VEGF, and M-CSF in LPS-induced macrophages.

• Proceedings of the Korean Society of Computer Information Conference
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• 2012.07a
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• pp.87-88
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• 2012

Because intra prediction modes in H.264 are determined by the brightness continuity between neighboring blocks, they can be used as a method for extracting edge information in the compression domain. However, if we just consider 9 intra prediction modes in H.264 as 9 different edge directions, we have the following two problems. First, intra prediction modes tend to yield too many edge blocks, generating unnecessary edge information. Second, we may not need all 9 directional edges (including the DC type) in H.264 intra prediction modes. For example, the EHD (edge histogram descriptor) in MPEG-7 defines only 4 directional edge types, namely horizontal, vertical, diagonal (HVD) edges with $0^{\circ}$, $90^{\circ}$, $45^{\circ}$, and $135^{\circ}$. Here, semi-diagonal (SD) edge types with $112.5^{\circ}$, $157.5^{\circ}$, $22.5^{\circ}$, and $67.5^{\circ}$ in the intra prediction modes in H.264 are not used. In this paper. we prepose a method that removes unnecessary edges from the intra prediction modes by utilizing the total average coefficient of 4x4 blocks in each slice and assign SD edges to HVD (horizontal, vertical, diagonal, $0^{\circ}$, $90^{\circ}$, $45^{\circ}$, $135^{\circ}$) edges by the contextual information of the neighboring blocks. Experimental results show that the edges determined by the proposed method in the compression domain are comparable to those of the previous edge detection methods in the spatial domain.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2008.11a
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• pp.267-270
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• 2008

H.264|AVC는 인트라 부호화 효율을 높이기 위해 공간 영역에서 주변 화소를 이용하여 다양한 방향에 대한 율-왜곡 최적화 기법을 사용하여 최적의 인트라 예측 모드를 선택한다. 하지만 율-왜곡 최적화 기법을 사용함에 따라 인트라 부호화에 높은 복잡도가 필요하게 되었다. 따라서 본 논문에서는 인트라 예측 모드 결정의 연산 복잡도를 감소시키고자 사전에 인트라 4x4 예측 모드들의 SATD(Sum of Absolute Transform Difference)를 계산하여 조기에 최우선 모드(Most Probable Mode)를 선택하는 방법을 제안하고, SATD의 값에 따라 제한된 후보 모드에 대해서만 율-왜곡 최적화를 수행하여 연산 복잡도를 감소하는 방법을 제안한다. 또한 Vertical, Horizontal 그리고 DC모드는 인트라 $4{\times}4$와 인트라 $16{\times}16$의 공통적인 모드이므로 인트라 $4{\times}4$에서 계산되어진 SATD값을 이용하여 인트라 $16{\times}16$에서의 SAD 계산 복잡도를 줄이는 방법을 제안한다. 본 논문에서 제안하는 빠른 인트라 예측 모드 결정 기법은 연산 복잡도는 평균 61.4% 감소 시킨 반면 부호화 손실은 평균 3.09%에 불과하였다.

• The Journal of Korean Medicine
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• v.33 no.4
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• pp.42-49
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• 2012

Objectives: The purpose of this study was to investigate effects of Red Ginseng-Ejung-tang Water Extract (ER) on cytokine production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Methods: Levels of various cytokines such as interleukin (IL)-6, IL-10, IL-2, IL-12p70, vascular endothelial growth factor (VEGF), monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-2, keratinocyte-derived chemokine (KC), tumor necrosis factor (TNF)-alpha, granulocyte macrophage colony-stimulating factor (GM-CSF) were measured by high-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. Results: ER significantly decreased levels of IL-6, IL-10, IL-2, IL-12p70, VEGF, and MCP-1 for 24 hrs incubation at the concentrations of 25, 50, and $100{\mu}g/mL$ in LPS-induced RAW 264.7 cells (P < 0.05). But ER did not exert significant effects on production of MIP-2, KC, TNF-${\alpha}$, and GM-CSF in LPS-induced RAW 264.7 cells. Conclusions: These results suggest that ER has an anti-inflammatory property related with its inhibition of cytokine production in LPS-induced macrophages.

• JSTS:Journal of Semiconductor Technology and Science
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• v.10 no.1
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• pp.37-44
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• 2010

New features of motion compensation, such as variable block size and multiple reference frames are introduced in H.264/AVC. However, these new features induce significant implementation complexity increases. In this paper, an efficient architecture for spiral-type motion estimation is proposed. First, we propose a hardware-friendly spiral search order. Then, an efficient processing element (PE) architecture for ME is proposed to achieve the proposed search order. The improved PE enables one-pixel-move of the reference pixel data to top, bottom, right, and left by four ports for input and output. Moreover, the parallel calculation architecture to calculate all block size with the SAD of 4x4 is introduced in the proposed architecture. As the result of hardware implementation, the hardware cost is about 145k gates. Maximum clock frequency is 134 MHz in the case of FPGA (Xilinx Vertex5) implementation.

• Archives of Pharmacal Research
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• v.11 no.2
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• pp.127-133
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• 1988

The crystal structure of fenbufen was determined by X-ray diffraction techniuques. The compound was recrystallized in orthorhombic crystal system, space group $Pca2_1$ a = 31. 918(10), b = 5.550 (2), c = 15.078 (9) ${\AA}, D_m$= $1.26, {\D_x$= 1.264 g/$\textrm{cm}^3$, and Z = 8. The structure was solved by direct methods and refined by least squares procedure to the final R value of 0.051 for 1780 independent reflections. Two molecules in the asymmetric unit are related by a pseudo center of symmetry and dimerized via O-H...O hydrogen bonds. The carbonyl group attached to the phenyl ring is nearly coplanar to the ring. In the crystal the molecules are arranged in paralle stacks in the a direction.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.79-82
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• 2008

We propose a new motion vector skip coding method for better motion compensation-based coding of inter-slices in H.264/AVC. It is to best utilize the spatial correlation between motion vectors of adjacent 4x4 blocks by effective motion vector coding. For this purpose, we introduce a new macroblock type of Predictive (P) slice into those of the H.264/AVC, so that it can lead to reduction in the coding bits required for encoding motion information. Experimental results with several well-known test video sequences verify that better performance of the proposed method is obtained.