• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2975-2979
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• 2015

Background: Telomere length is closely associated with cellular radiosensitivity and WRAP53 is required for telomere addition by telomerase. In this research we assessed radiosensitivity of laryngeal squamous cell carcinoma Hep-2 cell lines after WRAP53 inhibition, and analyzed the molecular mechanisms. Materials and Methods: phWRAP53-siRNA and pNeg-siRNA were constructed and transfected into Hep-2 cells with lipofectamine. Expression of WRAP53 was analyzed by RT-PCR and Western-blottin, radiosensitivity of Hep-2 cells was assessed colony formation assay, and the relative length of telomeres was measured by QPCR. Results: The data revealed that the plasmid of phWRAP53-siRNA was constructed successfully, and the mRNA and protein levels of WRAP53 were both obviously reduced in the Hep-2 cell line transfected with phWRAP53-siRNA. After Hep-2 cells were irradiated with X-rays, the $D_0$ and $SF_2$ were 2.481 and 0.472, respectively, in the phWRAP53-siRNA group, much lower than in the control group ($D_0$ and $SF_2$ of 3.213 and 0.592) (P<0.01). The relative telomere length in the phWRAP53-siRNA group was $0.185{\pm}0.01$, much lower than in the untreated group ($0.523{\pm}0.06$) and the control group ($0.435{\pm}0.01$). Conclusions: Decreasing the expression of WRAP53 using RNA interference technique can enhance the radiosensitivity of Hep-2 cell lines by influencing the telomere length. WRAP53 is expected to be a new target to regulate the radiosensitization of tumor cells.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3503-3507
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• 2013

Background: Although the majority of investigations concerned with TP53 and its protein have focused on coding regions, recently a set of studies highlighted significant roles of regulatory elements located in p53 mRNA, especially 5'UTR. The wrap53${\alpha}$ transcript is one of those that acts as a natural antisense agent, forming RNA-RNA hybrids with p53 mRNA and protecting it from degradation. Materials and Methods: In this study, we focused on the mutation status of exon $1{\alpha}$ of the WRAP53 gene (according to exon 1 of p53) in 160 breast tumor tissue samples and conducted a bioinformatics search for probable miRNA binding site in the p53/wrap53 overlapping region. Mutations were detected, using single stranded conformation polymorphism (SSCP) and sequencing. We applied the miRBase database for prediction of miRNAs which target overlapping region of p53/wrap53 transcripts. Results: Our results showed all samples to have wild type alleles in exon 1 of TP53 gene. We could detect a novel and unreported intronic mutation (IVS1+56, G>C) outside overlapping regions of p53/wrap53 genes in breast cancer tissues and also predict the presence of a binding site for miR-4732-5p in the 5'UTR of Wrap53 mRNA. Conclusions: From our findings we propose designing further studies focused on overexpression of miRNA-4732-5p and introducing different mutations in the overlapping region of wrap53 and p53 genes in order to study their effects on p53 and its ${\Delta}N$ isoform (${\Delta}$40p53) expression. The results may provide new pieces in the p53 targeting puzzle for cancer therapy.

• Food Science and Preservation
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• v.5 no.4
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• pp.315-319
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• 1998

In order to study the effect of packing materials on the quality of fares during storage period, grapes (Campbell Early) were packed with different materials such as expendable folystyrene (EPS) box, paper board box, biopaper board box, paper board box + small box, EPS box + (EPS dish + Bio-PE film sealing), md EPS box + (EPS dish + wrap sealing). The fruit weight loss was increased with the storage period by all the treatments. Weight loss was 6.38% lower in the EPS box+ (EPS dish + Bio film sealing) during 15 days of storage and 5.53% lower in EPS box + (EPS dish + wrap sealing) than that in the EPS box. The abnormal fruits were more increased in the sealing packing than in the non-sealing packing since water transpiration was prevented in the sealing treatment. Wilting fruits were also fewer in the sealing packing than that in the non-sealing treatment. The taste and appearance quality were worsened with increasing the storage days, whereas the appearance quality of the grapes in the bio paper board box was better. Hardness was scarecely changed in the EP5 box+ (EPS dish + Bio-PE film sealing) treatment than those by the other treatments. The soluble solid and acidity showed very little change but soluble solid content was more decreased in the sealing packing than that by the non-sealing treatment.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.14 no.2
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• pp.53-56
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• 2008

Polyvinylchloride(PVC) is variously used to produce food packaging, wrap film, sealing compound of drinking bottle cap. Safety of these PVC food package is controlled by Food Code, PVC regulation. This study was carried out researches on manufacture process, physical, chemical properties and monomer(CAS No., common name, molecular structure) of PVC to help the understanding of PVC material. Also, this study helps us the understanding of regulation through researches on the foundation of PVC regulation, the purpose of test and the comment of test method. It is considered that these informations of material properties and explanations of test method are very useful to PVC producers, users and analyzers of them.

• Journal of the Korea Organic Resources Recycling Association
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• v.7 no.1
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• pp.53-66
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• 1999

Limited landfill area and finance as well as a large generation of food wastes, have called for systematic approaches towards potential cost savings. In this study, the regional management system of food wastes generated from the residential and commercial sources was considered in three cities bounded each other; Changwon, Masan, and Jinhae city. Total thirteen alternatives were established and compared by applying the WRAP (Waste Resource Allocation Program). The following results were obtained: 1. While relatively small amounts were generated from the commercial sources such as cafeteria, restaurants, and market facilities, almost 80% of food wastes were generated from the residential sources. 2. Unit costs for food waste management in three cities were different according to their present situation such as the type and location of major generation sources and treatment facilities. Especially, the highest cost appeared in Jinhae city due to the most expensive construction of coastal landfill site. 3. Considering proper revenue, the whole conversion of food waste into animal feed was selected as the optimal alternative and represented 60% to 74% of the management cost of the whole landfill alternative in all cities. Comparing the other alternatives, composting of food waste was more economical than landfill alternative and the incineration was the most expensive alternative. 4. Some of the regional management systems using common food waste processing facilities together in three cities showed to be more economical than the single management system. Therefore, more detailed research for the regional management systems of food waste was recommended.

• Journal of environmental and Sanitary engineering
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• v.20 no.2 s.56
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• pp.39-46
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• 2005

This study was Performed to survey and evaluate the contents of Plasticizers such as DEP(diethyl phthalate), DPrP(di-n-Phthalate), DBP(di-n-butyl Phthalate), DPP(di-n-pentyl Phthalate), DCHP(dicyclohexyl phthalate), BBP (butylbenzyl phthalate), DEHP(di-(2-ethylhexyl) phthalate) and DEHA(di-(2-ethylheryl) adipate), which are suspected as endocrine disruptors, in food and drug PVC packaging. Tested samples were 5 food wraps, 35 food containers, 40 food and drug packages(type of tablet and capsule) in Gyeonggi-Do area. The contents of DEHA in wrap were 188.9g/kg, 203.1g/kg, 238.4g/kg, 290.9g/kg and 308.3g/kg, respectively, while the other plasticizers were not detected. DEHP was used in 4 samples of food containers and DEHP contents were 4.7g/kg, 30.7g/kg, 35.8g/kg and 53.4g/kg, respectively. In food and drug packaging materials(type of tablet and capsule), the plasticizers were not detected.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.3-13
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• 1994

To minimize the time ordinarily spent in mono filter cigarette design, we studied the relationship between major seven independant variables ; filament(X1) and total denier(X2), porosity of the aller plug wrap(X3), filter length(X4), Porosity of the tip paper(X5) and cigarette paper(X6) and net weight of the reference cut tobacco(X7). Ninty trial numbers were obtained as a results of using rotatable central composite design and it is analyzed by the multiple regression analysis with stepwise in SAS/pc under restricted conditions. That is, UPD (Y1) = 82.96 - 3.80X1 + 2.50X2 - 3.29X3 - 3.15X5 - 0.83X22 + 1.88X5X6 - 1.38 X5X7(R2: 0.63), EPD(Y2) : 120.91 - 5.70X1 + 3.60X2 + 4.23X4 - 0.93X6 + 4.06X7 (R2=0.84), TVR(Y3) = 49.70 - 0.78X1 + 3.60X3 + 2.00X4 + 4.20X5 - 0.93X6 + 2.64X7 - 1.07X1X2 + 1.0IX1 X3 + 1.05X2X6 + 0.45X22 - 0.64X42 + 1.29X4X6 - 0.97X4X7 - 1.28X5X6 + 1.53X5X7 + 1.39X6X7(R2=0.65), and EVR(Y4) : 3.24-0.21X3-0.20X4 -0.24X5+0.67X6+0.26X4X7 (R2=0.55), where EPD : encapsulated pressure drop, VPD : unencapsulated pressure drop, TVR ; tip ventilation rate, and En : envelope ventilation rate. All variables in the model are significant at the 0.05 level.

• Journal of Fashion Business
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• v.12 no.3
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• pp.31-53
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• 2008

This study is to suggest a brassiere pattern suitable to the Chinese woman by recognizing the differences among the brassiere patterns of Europe, America and Italy by comparison. Collected data was analyzed by using SPSS 12.0. 1. If seeing the length of the ordinary brassiere's wing in Korea, the mean is 31.02cm to be shorter than these 3 patterns. The length of a wing should be adjusted according to the degree of fabric's elasticity. 2. The ESMOD type shows a high angle of $60^{\circ}$ as the position of a shoulder strip is placed outward a lot compared with other patterns. 3. For the FIT and Marangoni types, the lengths of the inside and the external diameters show similarly to each other but the ESMOD type shows that its inside diameter is 7.5cm and external one is 9.6cm to have the difference of 2.1cm so that it is considered to put the bust together stronger than the others. 4. The cup circumference of the FIT pattern shows to be the biggest and that of the FIT pattern is the smallest. As the FIT pattern has the shape to wrap the side of the bust, it has large circumference but as it has a narrow angle of a dart, it seems to be fit to the woman with a small and flat bust. 5. For 1/2 of the nipple distance of the brassiere for the Korean adult women, it shows to be 6.12cm, much narrower than the patterns studied, and it is noticed that the ESMOD pattern is very similar to the brassier in the Korean market. 6. As the mean keeper height is 6.5cm, it is noticed that it is very similar to the brassier for the Korean adult women if comparing that the brassiere for the Korean adult has the keeper height of 6.5-8.6cm. The Marangoni pattern tends to have a little low and the ESMOD and FIT patterns have a ordinary measure.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.303-308
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• 2015

The consumption of leaf vegetables has been steadily increasing in Korea. Leaf vegetables are used for "Ssam (vegetable wrap-up), eaf vegetables has been steadily increasing in Korea. Leaf vegetables are used for asoned condiments inside several layers of young vegetable leaves. This study investigated the effect of air temperature on the growth and phytochemical contents of beet (Beta vulgaris L.) and Ssamchoo (Brassica lee L. ssp. namai) grown in a closed-type plant factory system where fluorescent lamps were used as an artificial light source. Seeds of beet and Ssamchoo were sown in a peat-lite germination mix. The roots of 20-day-old seedlings were washed, and the seedlings were planted on a styrofoam board and grown in hydroponic beds for 25 days under fluorescent light. Plants were exposed to one of three different air temperature regimes (20, 25 and $30^{\circ}C$ during the day combined with $18^{\circ}C$ during the night), which were monitored with a sensor at 30 cm above the plant canopy. Increased plant height and leaf area were observed in beet at $25^{\circ}C$ and $30^{\circ}C$ compared to $20^{\circ}C$. For Ssamchoo, the greatest plant height, leaf area, fresh weight and dry weight were obtained at $20^{\circ}C$. Ascorbic acid content of beet and Ssamchoo leaves were highest at $30^{\circ}C$. In beet, total polyphenol and flavonoid contents were higher at $20^{\circ}C$ (42.4, $197.0mg{\cdot}g^{-1}DW$) and $25^{\circ}C$ (46.9, $217.0mg{\cdot}g^{-1}DW$) than $30^{\circ}C$ (22.4, $88.0mg{\cdot}g^{-1}DW$). In Ssamchoo, total polyphenol and flavonoid contents were also higher at $20^{\circ}C$ (79.2, $268.2mg{\cdot}g^{-1}DW$) and $25^{\circ}C$ (66.3, $258.3mg{\cdot}g^{-1}DW$), respectively, than $30^{\circ}C$ (53.7, $134.7mg{\cdot}g^{-1}DW$). Hence, the optimum temperature appears to be $20^{\circ}C$ for growing both beet and Ssamchoo in a closed-type plant factory system with fluorescent light.

• Journal of Korean Home Economics Education Association
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• v.26 no.1
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• pp.31-53
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• 2014

The purpose of this study is to analyze how the elements of activating happiness education suggested by Noddings is reflected in the Home Economics part of Middle School Technology-Home Economics Textbook Volume 1 of 2009 Curriculum Revision. The introduction style of unit, sub-unit, and small chapter, the objectives, the body contents, the activity resources, the tables/diagrams/pictures, the supplementry and advancedl materials, and the wrap-up and evaluation of sub-unit and units of Home Economics part of Technology Home Economics textbook volume 1 were analyzed. Noddings suggested the elements for activating happiness education in five areas of personal life sector including 'home making', 'place and nature', 'parenting', 'chracter and spiriual experiences', and 'growth of interpersonal relationships' and two areas of public one including 'preperation for work' and 'community, democracy and voluntary activities'. The specific elements in seven sectors of activating happiness education were extracted using the content analysis. How the elements of those suggested by Noddings were reflected in the various parts of the textbook were analyzed in terms of the closeness of approaches, contents, and procedures between Noddings's and textbook. The major findings of this study were as follows: 1. The degree to which the elements of activating happiness education were reflected in the textbook differed by each unit. The elements of activating happiness education were reflected the most frequently in the unit of 'Understanding Adolescence' and the least frequently in the unit of 'Self-management of Adolescence'. 2. Although the elements of activating happiness education were generally reflected in all the elements of a textbook, these elements were relatively more reflected in the body contents and tables/diagrams/pictures.