• Title/Summary/Keyword: worm

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Insecticidal Activities against Major Lepidopteran Pests and Culture Condition of Bacillus thuringiensis sp. aizawai collected in Korea (국내에서 선발한 Bacillus thuringiensis sp. aizawai 균주의 주요 나방류 해충에 대한 살충 활성 및 배양특성)

  • Lee, Sang-Guei;Choi, Kee-Hyun;Lee, Young-Su;Oh, Kyeong-Seok;O, Jeung-Hun;Choi, Sung-Won
    • The Korean Journal of Pesticide Science
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    • v.10 no.2
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    • pp.131-137
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    • 2006
  • This experiment was conducted to select prominent microorganisms with a good insecticidal activity among the ten species, which isolated from soil at the near of Chung-buk, Chung-nam, and Gang-won provinces and made protein crystal endotoxin. As a result, GB-413 strain was finally selected, which showed the high insecticidal activity against susceptible diamondback moth (Plutella xylostella), beet army worm (Spodoptera exigua) and tobacco cutworm (Spodoptera litura) as well as resistant diamondback moth strains. By modifying the cultivation process f.g. lowing the glucose concentration at early cultivation stage and adding the carbon after inducing the spores, the percentage of making spore as well as the number of active spore were increased and the time for cultivation and spore forming was reduced without a reduction of insecticidal activity. These results were not only applied successfully for the optimized cultivation process for a fermentation tank containing five tons capacity, but also improved the possibility of mass cultivation for commercial production.

Tropical plant supplementation effects on the performance and parasite burden of goats

  • Romero, Juan J.;Zarate, Miguel A.;Ogunade, Ibukun M.;Arriola, Kathy G.;Adesogan, Adegbola T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.2
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    • pp.208-217
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    • 2018
  • Objective: Examine the effects of supplementing bahiagrass hay (BG) with potentially anthelmintic quantities of hays of perennial peanut (PEA) or sericea lespedeza (LES) or seeds of velvet bean (Mucuna pruriens L.; MUC) or papaya (PAP) on the intake and nutritive value (Experiment 1), and the performance and parasite burden (Experiment 2) of goats. Methods: In Experiment 1, 38 male goats ($27.4{\pm}5.7kg$ body weight) were randomly assigned to each of 5 treatments: i) BG alone and BG plus; ii) PEA; iii) LES; iv) MUC; and v) PAP. Goats were fed for ad libitum consumption and adapted to the diets for 14 d followed by 7 d of measurement. The PEA, LES, MUC (50%, 50%, and 10% of the diet dry matter [DM], respectively), and PAP (forced-fed at 10 g/d) were fed at rates that would elicit anthelmintic effects. In Experiment 2, goats remained in the same treatments but were allocated to 15 pens (3 pens per treatment) from d 22 to 63. All goats were infected with parasites by grazing an infected bahiagrass pasture from 0800 to 1500 h daily and then returned to the pens. Results: Dry matter intake tended to be greater in goats fed PEA and LES than those fed BG (757 and 745 vs 612 g/d, respectively). Digestibility of DM (59.5% vs 54.9%) and organic matter (60.8% vs 56.0%) were greater in goats fed MUC vs BG, respectively. In Experiment 2, feeding PAP, LES, and PEA to goats reduced nematode fecal egg counts by 72%, 52%, and 32%, reduced abomasal adult worm counts by 78%, 52%, and 42%, and decreased plasma haptoglobin concentrations by 42%, 40%, and 45% relative to feeding BG alone, respectively. Conclusion: Supplementation with PEA, LES, and PAP decreased the parasite burden of goats but did not increase their performance. PAP was the most effective anthelmintic supplement.

Population-Based Intervention for Liver Fluke Prevention and Control in Meuang Yang District, Nakhon Ratchasima Province, Thailand

  • Kompor, Pontip;Karn, Rattikarn Muang;Norkaew, Jun;Kujapun, Jirawoot;Photipim, Mali;Ponphimai, Sukanya;Chavengkun, Wasugree;Paew, Somkiat Phong;Kaewpitoon, Soraya;Rujirakul, Ratana;Wakhuwathapong, Parichart;Phatisena, Tanida;Eaksanti, Thawatchai;Joosiri, Apinya;Polsripradistdist, Poowadol;Padchasuwan, Natnapa;Kaewpitoon, Natthawut
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.2
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    • pp.685-689
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    • 2016
  • Opisthorchiasis is still a major health problem in rural communities of Thailand. Infection is associated with cholangiocarcinoma (CCA), which is found frequently in Thailand, particularly in the northeastern. Therefore, this study aimed to evaluate the effectiveness of health intervention in the population at risk for opisthorchiasis and CCA. A quasi-experimental study was conducted in Meuang Yang district, Nakhon Ratchasima province, northeastern Thailand, between June and October 2015. Participants were completed health intervention comprising 4 stations; 1, VDO clip of moving adult worm of liver fluke; 2, poster of life cycle of liver fluke; 3, microscopy with adult and egg liver fluke; and 4, brochure with the knowledge of liver fluke containing infection, signs, symptoms, related disease, diagnosis, treatment, prevention, and control. Pre-and-post-test questionnaires were utilized to collect data from all participants. Students paired t-tests were used to analyze differences between before and after participation in the health intervention. Knowledge (mean difference=-7.48, t=-51.241, 95% CI, -7.77, -7.19, p-value =0.001), attitude (mean difference=-9.07, t=-9.818, 95% CI=-10.9, -7.24, p-value=0.001), and practice (mean difference=-2.04, t=-2.688, 95% CI=-3.55, -0.53, p-value=0.008), changed between before and after time points with statistical significance. Community rules were concluded regarding: (1) cooked cyprinoid fish consumption; (2) stop under cooked cyprinoid fish by household cooker; (3) cooked food consumption; (4) hygienic defecation; (5) corrected knowledge campaign close to each household; (6) organizing a village food safety club; (7) and annual health check including stool examination featuring monitoring by village health volunteers and local public health officers. The results indicates that the present health intervention program was effective and easy to understand, with low cost and taking only a short time. Therefore, this program may useful for further work at community and provincial levels for liver fluke prevention and control.

A Study on Public Characters : focused on Site-Specificity (장소특정성(Site-Specificity)을 중심으로 한 공공캐릭터에 관한 연구)

  • Ryu, Yu-hee
    • Cartoon and Animation Studies
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    • s.47
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    • pp.335-356
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    • 2017
  • With the development of the domestic character industry, the interest in public characters has also been increased as like the characters of industrial contents. The public character is a non-commercial character implicitly expressing the symbolism shared or directed by members of the specific region, organization, organization, organization, or event. It has been distinguished from commercial design, aiming the profit for a specific person or group, and it has been given the role coincided with the purpose of public design, pursuing social value such as citizens' happiness, happiness in the subject and object, aiming value and role of design. Public characters has been grown around the local government character development, but most of the characters were not practically utilized. However, as interest in public characters has been increased, those have been received worm welcome again as a means of expressing the uniqueness and identity of the region. In this paper, we have tried to study the expression of identity of public character by substituting the site-specificity. The site-specificity is referring the art of the time that focuses on the region in contemporary art, a work in which the meaning is created by a specific region and situation. Currently, as a main role of public character is representing the region or the institution, its identity and value can be judged by the community it belongs. Through this, it is intended to study the social meaning of the character in the region as a public design.

Studies on the chromosomes of trematoda, Fasciola spp, Eurytrema pancreaticum and Calicophoron calicophorum in Korea (흡충류인 간질, 췌질 및 칼리코포론쌍구흡충의 염색체에 관한 연구)

  • Jang, Du-hwan;Noh, Jae-wuk;Kang, Du-weon;Kim, Byung-ki;Kim, Sung-won
    • Korean Journal of Veterinary Research
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    • v.30 no.3
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    • pp.317-331
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    • 1990
  • Chromosomes of gonadal tissues from Fasciola spp, Eurytrema pancreaticum and Calicophoron calicophorum occurred Korean cattle were egamined using modified air-drying method. To compare their phenotype with three different genotypes among Fasciola spp, the adult and egg si2e were measured since they have been known as important taxonomical characters. The results obtained were as followed; Cattle liver fluke, Fasciola spp were classified into three types based on their chromosomal complements such as individual with 2o chromosome(diploid), 30 chromosome(triploid) and 20/30 mosaic constitution(mixoploid). The propotions of appearance of three types were 40.00%, 54.29% and 5.71%, respectively. The frequency of three types in type I which was regarded as F gigantica were 58.82% for diploid, 35.29% for triploid and 5.88% for mixoploid, but in type II which was regarded as F hepatica were 72.2% for triploid, 22.22% for diploid and 5.56% for mixoploid. Egg length of triploid forms was significantly larger than that of diploid forms and egg size of mixoploid forms was similiar to that of triploid forms. Worm size of triploid forms was larger than that of diploid forms and was more similar to that of mixoploid forms, but the statistical data were not significant. Diploid chromosome consisted of one pair of metacentric chromosome(m), four pairs of submetacentric chromosomes(sm), five pairs of subtelocentric chromosomes(st), while triploid chromosome consisted of one pair of metacentric chromosome, seven pairs of submetacen.tric chromosomes, one pair of subtelocentric chromosome and telocentric chromosome(t), respectively. In mixoploid chromosome, constitution of the chromosomes of diploid or triploid cell was consistent with that of diploid or triploid. Chromosomes of gonadal tissues from pancreatic fluke, Eurytrema pancreaticum consisted of 13 pairs of homologs(2n=26, n=13). The mitotic and meiotic divisions were observed frequently. In the mitotic metaphase, Karyotype consisted of five pairs of metacentric chromosomes, four pairs of submetacentric chromosomes, three pairs of subtelocentric chromosomes and one pair of telocentric chromosome. Chromosomes of gonadal tissues from stomach fluke, Calicvphoron calicophorum consisted of 9 pairs of homologs(2n=18, n=9). The meiotic divisions was frequently observed, but mitotic divisions was rare. In the mitotic metaphase, Karyotype consisted of two pairs of metacentric chromosomes, three pairs of submetacentric chromosomes and four pairs of subtelocentric chromosomes. Karyotype of Calicophoron calicophorum differed from that of Japanese C calicophorum which was similar to that of Paramphistomum cervi of Korean cattle. Though that of Calicophoron calicophorum of Korean cattle was similar to that of Paramphistomum explanatum of Korean cattle, that have been recognized to be a different species of fluke.

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Aroma Compounds and Antimicrobial Effect of Garlic from Different Areas in Korea (국내 산지별 마늘의 향기성분 및 항균활성)

  • Shin, Jung-Bye;Kim, Ra-Jeong;Lee, Soo-Jung;Kang, Min-Jung;Seo, Jong-Kwon;Sung, Nak-Ju
    • Food Science and Preservation
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    • v.18 no.2
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    • pp.199-207
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    • 2011
  • Physico-chemical characteristics such as width, weight, color and aroma compounds of garlic from 9 different areas were analyzed. Also, antimicrobial activity was tested for their juices. Width of garlic bulb was larger in growing worm-season garlics (Jeju, Namhae, Hapcheon, Hampyeong and Muan) than those of cool-season garlics (Taean, Seosan, Uiseoung) and imported from China. But numbers of nuts were smaller in growing cool-season garlics than others. L color levels of garlics were 80.73~87.40 and a color level have not significantly difference in all samples, b color level was lower in growing Namhae ($20.79{\pm}1.20$) and Muan ($20.91{\pm}1.62$). 25~30 peaks obtained from aroma compounds analysis of various garlics. Among these compounds, 17 kinds of aroma compounds were identified by GC-MS. And, we showed that 15 kinds of compounds were sulfur containing aroma compounds. Diallyl disulfide was contained 34.90~60.54% in various garlic and the highest contents than other compounds in total compounds. It was detected the highest in China garlic but the lowest in growing Muan garlic. All of garlic samples showed antimicrobial activity against Strep. mutans, B. subtilis, E. coli. V. parahaemolyticus, Asp. flavus, and C. albicans. Especially antimicrobial activity was stronger against E. coli, and C. albicans. Antimicrobial activity was enhanced by sample addition volume and the highest in Namhae garlic sample.

Effects of sewage sludge on the cocoon production and its hatchability of tiger worm(Eisenia fetida) (하수슬러지가 줄지렁이(Eisenia fetida)의 산란 및 부화율에 미치는 영향)

  • Park, Kwang-Il;Bae, Yoon-Hwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.4
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    • pp.105-111
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    • 2004
  • When the clitellate tiger worms(Eisenia fetida) were fed with paper mill sludge, sewage sludge of agricultural-urban area and sewage sludge of agricultural area, number of cocoons produced by individual clitellate earthworm for 104 days were 11.8, 8.6 and 3.5, respectively. However, clitellate earthworms that had been fed with sewage sludge of agricultural-urban area for 52 days or fed with sewage sludge of agricultural area for 95 days could not produce cocoons, whereas the earthworms that were fed with paper mill sludge kept producing cocoons through the investigating period. The hatchability of cocoons produced by the clitellate tiger worms(Eisenia fetida) fed with sewage sludge of agricultural-urban region, sewage sludge of agricultural region and paper mill sludge were 42.8%, 38.3% and 39.2%, respectively. And hatching periods of cocoons were 33.8~36.0 days, which were not affected by the kinds of sludges. However, the cocoons produced by clitellate earthworms which had been fed with sewage sludge for about 1.5 months could not hatch at all. Death rates of second generation hatched from cocoons produced by tiger worms(Eisenia fetida) which had been fed with the sewage sludge from agricultural-urban region and the sewage sludge from agricultural region reached to 100% and 90% at 60 days after hatching, respectively, whereas death rate of second generation fed with paper mill sludge reached to 16%.

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Pattern on Enterobius vermicularis in Korea (한국(韓國)에 있어서의 요충감염상(蟯虫感染相))

  • Lee, Joon-Sang;Kim, Kyu-Ho;Ryu, Jong-Cheol;Hong, Kyung-Sook;Lee, Hae-Jung;Rim, Han-Jong
    • Journal of agricultural medicine and community health
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    • v.3 no.1
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    • pp.18-26
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    • 1978
  • Enterobius vermicularis (pinworm) infestation in present day Korea has become one of nationwide problems. But, the incidence of pinworm infestation having been reported by many workers was almostly the data for specific groups. The incidence of pinworm infestation for 208 families of 760(male; 30, female;420) middle class inhabitant of Seoul, 238 subrural inhabitants in Gyeonggi Province and 150(male;77, female;73) rural primary students in three different districts were examined by modified Gralam's scotch-tape swab method. The results are summarized as follows; 1. The incidence of nationwide infestation a. The nationwide incidence of pinworm infestation of the 1,148 specimens examined was 28.7% (male; 27.5%, female; 29.6%). b. The highest incidence was found among 5-14 years of age. c. In comparison of the incidence by the area, the highest incidence was found among subrural area, next among rural area and the lowest among urban area. 2, Urban area a. The incidence of the 760 inhabitants examined was 23.2% (male; 24.1%, female; 22. 3%). b. The incidence of the parents group was 12.2% and children group was 30. 30.3%. c. In the parents who hold one or more pinworm poitive cases among children, the incidence of father group was 13.2% and mother group was 29.7%.3. Subrural area a. The incidence of the 238 inhabitants examined was 42.9% (male, 40.8%, female; 44.4%). b. The cumulative positive rate of anal swabs showed increasing tendency. c. Worm collection rates after chemotherapy of 98 inhabitants were 54, 0%. d. In scotch tape anal swab only one times, diagnostic value was 74.0%. e. Mother group infested with pinworm seemed to play an important role for the high incidence in individual family. f. In comparison of the incidence by the examination method, the highest incidence was found among scotch tape method. 4. Rural area The incidence of the 150 primary students examined was 34. 5% (male ; 24. 7%, female; 43.8%).

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Studies on insecticidal activity and synthesis of Bis (trichlorophenoxy) ethane (Bis(trichlorophenoxy) ethane(BTPE)의 합성 및 살충효력에 관한 연구(I))

  • Kim C. S.;Kim H. W.;Kim M. Y.;Kang S. W.;Lee D. S.;Lee E. S.
    • Korean journal of applied entomology
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    • v.4
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    • pp.47-50
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    • 1965
  • Many of the chlorophenol derivatives are widely used as insecticides, herbicides and plant growth regulators. The function and use of these chemicals would be different according to the number of chlorine and their chemical structures. It was reported in this article that 1) Bis(trichlorophenoxy) ethane was synthesized with 2 mol-trichlorophenol and 1 moi-dibromoethane in 2 moi-Sodium hydroxide alcohol solution, and 3) the insecticidal activity of Bis(trichlorophenoxy) ethane was compared with Phenkapton, Tedion V-18, BHC, and DDT, on Citrus red mite, Bombix mori (Silk worm), and Daikon leaf beetle(Phaedon brassicae) 3) The toxicity of Bis(2, 4, 5-trichlorophenoxy) ethane and Bis(2, 4, 5-trichlorophenoxy) ethane was studied on mice. The following results were obtained. 1) Yield of Bis(2, 4, 5-trichlorophenoxy) ethane, $50.06\%$, m.p. $157-159^{\circ}C$, and yield of Bis(2,4, 6-trichlorophenoxy) ethane, $32.60\%$, m.p. $162-163^{\circ}C$. 2) Insecticidal activity of Bis(2, 4, 5-trichlorophenoxy) ethane to Citrus red mite is stronger than that of Tedion V-18, and weaker than that of Phenkapton. 3) Insecticidal activity of Bis(2, 4, 5-trichlorophenoxy) ethane to Bombix mori is weaker than those of BHC and DDT. 4) Insecticidal activity of Bis(2. 4, 5-trichlorophenoxy) ethane to Daikon leaf beetle proved to be ineffective. 5) Five rams of Bis(2, 4, 5-trichlorophenoxy) ethane and Bis(2, 4, 6-trichlorophenoxy) ethane pet kg of body weight respectively were given to mice orally, and none of the mouse was killed by it after a period of 72 hours. Therefore it seems that there is almost no toxicity.

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Occurrence of Lepidopteran Insect Pests and Injury Aspects in Adzuki Bean Fields (팥 포장에서 나비목 해충의 발생과 피해 양상)

  • Jung, Jin-Kyo;Seo, Bo-Yoon;Cho, Jum-Rae;Kwon, Yun-Hee;Kim, Gil-Ha
    • Korean journal of applied entomology
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    • v.48 no.1
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    • pp.29-35
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    • 2009
  • Three lepidopteran insect pests of the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), the soybean pod worm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and an Ostrinia spp. (Lepidoptera: Pyralidae) attacking adzuki bean, Vigna angularis, were confirmed as the major insect pests during the reproductive developmental stage of adzuki bean, and M. vitrata existed dominantly. Almost all plants in the adzuki bean field were injured by at least one of the three species, and $15{\sim}60%$ of reproductive organs were injured. Unbloomed inflorescence occurred continuously through the reproductive stage of adzuki bean. While the flower was many at the early stage and its number soon decreased, the pod was few at the early stage, but its number increased soon and maintained at a constant level. The results suggested that the adzuki bean injured by insect pests compensate the flower loss by developing new inflorescence, but the compensation do not cause the development of new pods. While M. vitrata and M. phaseoli were observed in flowers, pods and stem mainly during the first half of reproductive stage of adzuki bean, Ostrinia spp. was observed only in pods and stem during the second half. In addition, while all instars of larvae of M. vitrata were observed, larvae between the third and fifth instars for M. pahseoli and Ostrinia spp. were observed.