• 환경생물
• /
• 제23권2호
• /
• pp.152-156
• /
• 2005

Ionizing radiation is a well- known therapy factor for human carcinoma cells. Genotoxic stress mediates cell cycle control, transcription and cellular signaling. In this work, we have used a microarray hybridization approach to characterize the cell type-specific transcriptional response of human carcinoma MCF-7 and HeLa cell line to $\gamma-radiation$, such as 4Gy 4hr. We found that exposure to $\gamma-ray$ alters by at least a $log_2$ factor of 1.0 the expression of known genes. Of the 27 genes affected by irradiation, 11 are down- regulated in MCF-7 cells and 2 genes induced by radiation,15 are repressed in HeLa cells. Many genes were involved in known damage- response pathways for cell cycling, transcription factor and cellular signaling response. However, in MCF-7 cells, we observed gene expression pattern in chromatin, apoptosis, stress, differentiation, cytokine, metabolism, ribosome and calcium. In HeLa cells, it showed clearly the expression changes in adhesion and migration, lysosome, brain, genome instability and translation. These insights reveal new therapy directions for studying the human carcinoma cell response to radiation.

• 한국전기전자재료학회논문지
• /
• 제21권6호
• /
• pp.499-502
• /
• 2008

In this work, Ga-doped ZnO (GZO) thin films for gas sensor application were deposited on low temperature co-fired ceramics (LTCC) substrates, by RF magnetron sputtering method. The LTCC substrate is one of promising materials for this application since it has many advantages (e.g., low cost production, high manufacturing yields and easy realizing 3D structure etc.). The LTCC substrates with thickness of $400\;{\mu}m$ were fabricated by laminating 12 green tapes which consist of alumina and glass particle in an organic binder. The structural properties of the fabricated GZO thin film with thickness of 50 nm is analyzed by X-ray diffraction method (XRD) and field emission scanning electron microscope (FESEM). The film shows good adhesion to the substrate. The GZO gas sensors are tested by gas measurement system and show fast response and recovery characteristics to $NO_x$ gas that is 27.2 and 27.9 sec, recpectively.

• 마이크로전자및패키징학회지
• /
• 제27권3호
• /
• pp.9-19
• /
• 2020

기능성 나노박막을 손상 없이 목표기판으로 옮기는 전사 기술은 나노박막 기반의 차세대 응용 제품 개발을 위한 초석이다. 본 논문에서는 최근 나노박막 전사의 연구 동향을 박막-기판 계면의 박리 원리에 따라 습식 식각 전사, 전기화학적 박리, 기계식 전사 방법 세 가지로 분류하여 간단하게 살펴볼 것이다. 더 나아가, 손쉽고, 기판 재활용이 가능하며, 광범위한 적용 가능성을 가지고 있어 유망 기술로 간주되는 기계식 전사 방법에 대하여 파괴 역학적 관점에 초점을 맞추어 다룰 것이다. 마지막으로, 나노박막의 기계식 전사 방법의 기술 성숙도를 향상시키기 위한 향후 도전 과제와 방향성에 대하여 고찰하고자 한다.

• Transactions on Electrical and Electronic Materials
• /
• 제3권1호
• /
• pp.9-13
• /
• 2002

In this work, the surface of inorganic fillers were modified with some functional groups such as stearic acid, aliphatic long chain, vinylsilane and aminosilane to control the interaction between inorganic fillers and polymer matrix. Ethylene-vinyl acetate copolymers (EVA) with various amount of vinyl-acetate and copolyether-ester elastomer were used as polymer matrix. The addition of inorganic fillers increases flame retardancy, but results in steep drop of electrical and mechanical properties, which may be caused by the defects in the interface between organic/inorganic hybrid composites. The hybrid composites are found to show better mechanical properties and higher volume resistivities as inorganic fillers are well dispersed and have good adhesion with polymer matrix. Also, the most effective type of functional group coated on fillers depends on the chemical structure of polymer.

• Structural Engineering and Mechanics
• /
• 제70권4호
• /
• pp.395-405
• /
• 2019

In the present work, the effects of cutting parameters on surface roughness parameters (Ra), tool wear parameters (VBmax), tool vibration (Vy) and material removal rate (MRR) during hard turning of AISI 4140 steel using coated carbide tool have been evaluated. The relationships between machining parameters and output variables were modeled using response surface methodology (RSM). Analysis of variance (ANOVA) was performed to quantify the effect of cutting parameters on the studied machining parameters and to check the adequacy of the mathematical model. Additionally, Multi-objective optimization based desirability function was performed to find optimal cutting parameters to minimize surface roughness, and maximize productivity. The experiments were planned as Box Behnken Design (BBD). The results show that feed rate influenced the surface roughness; the cutting speed influenced the tool wear; the feed rate influenced the tool vibration predominantly. According to the microscopic imagery, it was observed that adhesion and abrasion as the major wear mechanism.

• 한국도서관정보학회지
• /
• 제9권
• /
• pp.115-144
• /
• 1982

In ordinary modern wester expression 'the Renaissance' was used to denote the impact made by dead Hellenism civilization in western Christendom, particularly Italian literary and artistic movement at Northern and central Italy in the late medieval period. However, A.J. Toynbee examined the renaissance from the different aspect of view. In his great work "A Study of History" in vol. IX, he succeeded in establishing the theory of historic civilization encounters in space and in time; and in time, civilization of the present and the past or between dead and infant successor contacts on the analogy of parenthood and sonship in the relation of A n.0, pparentation-and-Affiliation. The distinguished his view of 'Renaissance' was illustrated in the sense of encounters between a grown-up civilization and the 'ghost' of its long-dead predecessor. The renaissances (by the process of evocation of ghost of its parent society) has not only one single aspect of literary and artistic field but also in politics, law, science and philosophy, languages and literatures and visual arts, and religion. The main theme of this study is to examine the development of libraries and its historical meaning through Toynbee's literary renaissance. His renaissance of Languages and Literatures has three typical steps: They are: 1st step-to restive the dead literature's remains: 2nd step-to remaster their meaning: 3rd step-to reproduce them in counterfeits... Through its first and second steps, collecting and editing, annotating by compiling an anthology, thesaurus, lexicon or encyclopedia, and in its third step publishing mostly imitation of classics took place. Toynbee depicted the five outstanding eminent representatives of literary renaissance who had a n.0, ppeared on the state of history down to the time of writing. They are: Assurbanipal, Constantine prophyrogenitus, Yung Lo, K'ang Hsi, and Ch'ien Lung and the last four had all been emperors of imperia rediviva. As the result of the examination of these five emperors with three steps of literary renaissance, the common result may be summarized as follows: 1. Those emperors of imperia rediviva interested in intellectual work and study, they also were deeply involved in collecting classics in an ostensible reason. 2. There were strong political intention of collecting materials as an a n.0, ppeasement policy of civilization by transferring scholars energies to an intellectual field. 3. Under the rulers of a resuscitated universal state, the literary renaissance were a product of political plane and that the total size of collection and work were huge. 4. Since there were strong exercise of sovereign power, an active censorship by distortion and elimination was inevitable. 5. There existed newly developed strained atmosphere between grown-up and long-dead parent civilization, whenever the book collection movement had occurred. 6. Over adhesion to the parent civilization caused imitation of classic work and the creative activities were stagnated.stagnated.

• Clinical and Experimental Reproductive Medicine
• /
• 제12권1호
• /
• pp.47-70
• /
• 1985

This study was presented of the 1,872 cases of infertile couples who visited and examined at the sterility clinic of Department of Obstetrics & Gynecology, Seoul National University Hospital from Sept., 1980 to Dec., 1983. Age, duration of infertility, past medical history, and other general factors were analyzed, and the factors responsible for infertility were classified and discussed. Mode of treatment, outcome of pregnancy, pregnancy rate responsible for each factor were also presented. The results were as follows: 1) The infertility was primary in 1,128, or 60.3% and secondary in 744, or 39.7%. 2) The age between 26 and 30 years of age comprised about one half of the total patients. 3) The duration of infertility between 1 and 4 years comprised about three quarters of the total patients, and the mean duration was 3.8 years. 4) The most common medical history in primary infertility was tuberculous disease, and that in secondary infertility was history of previous laparotomy. 5) About two thirds of antecedent pregnancies were abortion. 6) The major etiologic factor of infertility were male factor in 12.3%, tubal factor in 38.8%, ovulatory failure in 25.4%, uterine factor in 8.8%, cervical factor in 5.2%, peritoneal factor in 9.5%, and no demonstrable cause in 11.3%. 7) The types of male factor were azoospermia in 61.6%, oligospermia in 25.8%, low motility in 11.6%, and other abnormality in 1.0%. 8) The types of ovulatory failure were ovarian failure in 7.4%, hypothalamo-pituitary failure in 8.1 %, hypothalamo-pituitary dysfunction (including Polycystic ovarian syndrome) in 30.2%, and hyperprolactinemia in 22.4%. 9) The types of uterine factor were endometrial tuberculosis in 27.5%, uterine synechia in 33.8%, uterine anomaly in 19.7%, myoma and polyp in 9.1 %, and luteal phase defect in 9.9%. 10) The types of peritoneal factor were pelvic adhesion in 80.9% and endometriosis in 19.6%. 11) Surgeries were done in 408 patients, and they were salpingolysis, lysis of extraadnexal adhesion, salpingostomy, fimbrioplasty, ovarian wedge resection for polycystic ovarian disease, tubo-tubal anastomosis, and tubo-uterine implantation in orders. 12) 243 pregnancies were achieved during the infertility work-up, of which livebirth was 46.5%, ectopic pregnancy was 7.4%, spontaneous abortion was 7.8%, and on-going pregnancy or lost to follow-up was 36.2%. 13) Pregnancy rates in various factors were male factor in 18.7%, ovulatory factor in 31.7%, tubal factor in 24.2%, uterine factor in 34.6%, cervical factor in 19.0%, peritoneal factor in 29.0%, combined factors in 10.5%, and unexplained infertility in 37.1%. Pregnancy rate in whole patients was 25.2%.

• 접착 및 계면
• /
• 제4권3호
• /
• pp.33-40
• /
• 2003

본 연구에서는 이관능성 에폭시 (diglycidyl ether of bisphenol A. DGEBA)와 Polyetherimide(PEI) 블랜드를 Amime계 경화제인 DDM을 이용하여 경화를 시켰으며, PEI의 함량 변화가 순수 에폭시 수지의 열적특성과 파괴인성에 미치는 영향에 대해 고찰하였다. 경화된 시편의 열적특성은 DSC에 의한 열분석을 통해 Kissinger 식을 이용하여 경화 활성화 에너지 ($E_a$)를 구하였고, 파괴인성은 크랙성장의 저항을 나타내는 임계응력세기 인자 ($K_{IC}$)을 측정하여 알아보았다. 시편의 파단특성은 주사전자현미경 (SEM)을 통해 조사하였으며, 기계적 계면특성을 알아보기 위해 contact angle로 표면자유에너지를 측정하였다. 실험 결과 DGEBA/PEI 블랜드계의 $E_a$, $K_{IC}$는 PEI 함량이 7.5 phr에서 최대값을 보였다. 이는 PEI 도입에 따른 치밀한 네트워크 구조가 증가했기 때문으로 판단되어 진다. 또한 DGEBA/PEI 블랜드계의 표면자유에너지는 $K_{IC}$와 유사한 경향을 보였으며, 이는 에폭시의 하이드록실기와 PEI의 imide 그룹 사이의 수소결합에 의해 극성요소가 증가되었기 때문으로 판단되어진다.

• Journal of Microbiology and Biotechnology
• /
• 제23권9호
• /
• pp.1308-1321
• /
• 2013

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average $1.85{\pm}0.11{\times}10^6$ cells/ml against $0.60{\pm}0.08{\times}10^6$ cells/ml for CultiSpher-S), mAb production ($2.04{\pm}0.41{\mu}g/ml$ against $0.99{\pm}0.35{\mu}g/ml$ for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, $4{\times}10^5$ cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.

• 접착 및 계면
• /
• 제15권3호
• /
• pp.116-122
• /
• 2014

자외선 경화법을 이용한 강판의 표면 코팅 시 사용되는 올리고머, 단량체, 광개시제 및 강판의 특성 등 주요 인자가 코팅 표면의 $20^{\circ}$ 및 $60^{\circ}$ 광택도(ASTM D523)에 미치는 영향에 대해서 연구하였다. 공정 변수에 대한 광택도의 변화는 $60^{\circ}$ 광택도에 비해 $20^{\circ}$ 광택도가 민감하게 변화하였다. 단독으로 올리고머를 사용할 경우의 경우, 광택도의 변화는 거의 없었으나, 올리고머의 혼합 비율이, 단량체의 종류와 혼합 비율, 올리고머와 단량체의 혼합 비율, 개시제의 종류와 함량 비, 강판의 종류는 광택도에 큰 영향을 주었다. 올리고머 혼합의 경우 폴리우레탄 아크릴레이트(UA)와 에폭시 아크릴레이트(EA)의 혼합 비율이 70 : 30, 단량체의 경우 트리메틸올프로판트리아크릴레이트(TMPTA)와 테트라히드로퍼퍼릴아크릴레이트(THFA)가 5 : 5의 비율로 혼합된 경우가, 혼합 올리고머(UA : EA = 70 : 30)의 함량이 90 wt%인 경우 최대의 광택도를 나타내었다. 광개시제 중 액체 타입인 Darocur MBF가 고체 타입인 Irgacure 184에 비해 우수한 광택도를 나타내었으며, 최소 투입량인 1 wt%의 농도에서 높은 광택도를 나타내었다. 기질의 경우, EG강판이나 primer가 처리된 강판에 비해 GI강판이 더 좋은 광택도를 보여주었다. 상기 공정 변수에 대한 연구를 통해 광택도 향상을 위해 사용되는 첨가제 없이도 $20^{\circ}$ 광택도 95 GU의 최대값을 $60^{\circ}$ 광택도는 120 GU의 광택도를 얻을 수 있었다.