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Enhancement of Hyaluronic Acid Production by Batch Culture of Streptococcus zooepidemicus via the addition of n-Dodecane as an Oxygen Vector

  • Liu, Long;Yang, Haiquan;Zhang, Dongxu;Du, Guocheng;Chen, Jian;Wang, Miao;Sun, Jun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.6
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    • pp.596-603
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    • 2009
  • This study aimed to examine the influence of adding an oxygen vector, n-dodecane, on hyaluronic acid (HA) production by batch culture of Streptococcus zooepidemicus. Owing to the high viscosity of culture broth, microbial HA production during 8-16 h was limited by the oxygen transfer coefficient $K_La$, which could be enhanced by adding n-dodecane. With the addition of n-dodecane to the culture medium to a final concentration of 5% (v/v), the average value of $K_La$ during 8-16 h was increased to $36{\pm}2h^{-1}$, which was 3.6 times that of the control without n-dodecane addition. With the increased $K_La$ and dissolved oxygen (DO) by adding 5% (v/v) of n-dodecane, a 30% increase of HA production was observed compared with the control. Furthermore, the comparison of the oxygen mass transfer in the absence and presence of n-dodecane was conducted with two proposed mathematical models. The use of n-dodecane as an oxygen vector, as described in this paper, provides an efficient alternative for the optimization of other aerobic biopolymer productions, where $K_La$ is usually a limiting factor.

A Study on Oxygen Saturation, Vital Signs, and Vomiting by Routine Suctioning to Healthy Newborns at Nursery (정상 신생아의 출생 후 흡인에 따른 산소포화도, 활력징후 및 구토)

  • Choi, Hye-Mi;Lee, Ja-Hyung
    • Child Health Nursing Research
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    • v.16 no.2
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    • pp.128-135
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    • 2010
  • Purpose: This study aimed to confirm the effect of routine suctioning at a nursery for healthy newborns who have undergone immediate oronasopharyngeal bulb suctioning after birth in a delivery room through the observation of their oxygen saturation level, heart rate, respiration rate, the vomiting sign, and the number of instances of vomiting. Methods: Data were collected for 62 days from March 15 to May 15, 2009 at the nursery of a hospital located in Seoul. One hundred forty newborns were assigned to one of three groups: a no suction group, an oropharyngeal suction group, or a orogastric suction group. Collected data were analyzed with the SPSS WIN 15.0 program using ANOVA, cross tabulations and an independent 2-sample t-test. Results: Routine suctioning to healthy newborns resulted in decreasing oxygen saturation levels and increasing the heart and respiration rate regardless of the kind of suctioning. Stabilization of the oxygen saturation level and vital signs was also observed without suctioning. Conclusion: To prevent healthy newborns from the side effect of suctioning, selective suctioning is recommended.

Behavior of Oxygen Equilibrium Pressure in CRT Glass Melts doped with Sb and Ce ions from the Viewpoint of Fining

  • Kim, Ki-Dong;Kim, Hyo-Kwang;Kim, Jun-Hong
    • Journal of the Korean Ceramic Society
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    • v.44 no.8
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    • pp.419-423
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    • 2007
  • The behavior of oxygen gas participating in fining was observed in CRT (Cathode Ray Tube) glass melts doped with $Sb_2O_5\;or\;CeO_2$ by means of a yttria-stabilized zirconia (YSZ) electrode. The temperature dependence of the oxygen equilibrium pressure ($P_{o2}$) or the activity in both melts showed typical behavior corresponding to a theoretical redox reaction. In other words, the $P_{o2}$ value of melts with $CeO_2$ was lower than that of melts with $Sb_2O_5$ above $1250^{\circ}C$. The result implies that $Sb_2O_5$, is more efficient as a fining agent compared to $CeO_2$. On the other hand, melts from a batch containing $Sb_2O_5\;and\;KNO_3$ showed much higher $P_{o2}$ values compared to melts without $KNO_3$ above $1350^{\circ}C$. It is suggested that the addition of $KNO_3$ to CRT glass batch contributes partly to the first fining of the melts.

Cochlodinium Red Tide Effects on the Respiration of Abalone, Haliotis discus hannai Ino

  • Seo, Kyung-Suk;Lee, Chang-Kyu
    • ALGAE
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    • v.22 no.3
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    • pp.241-246
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    • 2007
  • Cochtodinium votykrikoides -related red tide is the most notorious tidal bloom, resulting in mass mortality to marineanimals. This study aimed to test the effect of C. polyknkoides on the lethality to Haliotis discus hannai under con-trolled conditions. The oxygen demand of C. polykrikoides increases to reach its peak duhng the night, while the oxy-gen usage by H. discus hannai was continuously decreased with a threshold of 2 mg L U. The addition of C.polykrikoides did not effect the respiration of the H. discus hannai. However, the usage of oxygen by C. polykrikoidesduhng the night may lead to anoxia in the animal. With aeration, the level of dissolved oxygen (D.O.) was between6.06 and 7.28 mg LU; 90% of abalones survived even with a high concentration of C. potykrikoides (9000 cells mL U).Without aeration (3 mg LU of D.O.), however, the H. discus hannai suffocated immediately. Once 20 hours hadelapsed, all of the abalones were dead. The density of the H. discus hannai population contributed to their mortality.Therefore, aeration during the night and maintaining lower abalone densities is the best way to promote the sur-vivorship of H. discus hannai during a C. polykrikoides red tide.

Metabolic Analysis of Poly(3-Hydroxybutyrate) Production by Recombinant Escherichia coli

  • WONG, HENG HO;RICHARD J. VAN WEGEN;JONG-IL CHOI;SANG YUP LEE
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.593-603
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    • 1999
  • Poly(3-hydroxybutyrate) (PHB) production by fermentation was examined under both restricted- and ample-oxygen supply conditions in a single fed-batch fermentation. Recombinant Escherichia coli transformed with the PHB production plasmid pSYLl07 was grown to reach high cell density (227 g/l dry cell weight) with a high PHB content (78% of dry cell weight), using a glucose-based minimal medium. A simple flux model containing 12 fluxes was developed and applied to the fermentation data. A superior closure (95%) of the carbon mass balance was achieved. When the data were put into use, the results demonstrated a surprisingly large excretion of formate and lactate. Even though periods of severe oxygen limitation coincided with rapid acetate and lactate excretion, PHB productivity and carbon utilization efficiency were not significantly impaired. These results are very positive in reducing oxygen demand in an industrial PHA fermentation without sacrificing its PHA productivity, thereby reducing overall production costs.

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MBE-growth and Oxygen Pressure Dependent Electrical and Magnetic Properties of Fe3O4 Thin Films

  • Dung, Dang Duc;Feng, Wuwei;Sin, Yu-Ri-Mi;Thiet, Duong Van;Jo, Seong-Rae
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.60-60
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    • 2011
  • Giant magnetoresistance (GMR), tunneling magnetoresistance (TMR), and magnetic random-access memory (MRAM) are currently active research areas in spintronics. The high magnetoresistance and the high spin polarization (P) of electrons in the ferromagnetic electrodes of tunnel junction or intermediate layers are required. Magnetite, Fe3O4, is predicted to possess as half-metallic nature, P ~ 100% spin polarization, and has a high Curie temperature (TC~850 K). Experiments demonstrated that the P~($80{\pm}5$)%, ~($60{\pm}5$)%, and ~40-55% for epitaxial (111), (110) and (001)-oriented Fe3O4 thin films, respectively. Epitaxial Fe3O4 films may enable us to investigate the effects of half metals on the spin transport without grain-boundary scattering.In addition, it has been reported that the Verwey transition (TV, a first order metal-insulator transition) of 120 K in bulk Fe3O4 is strongly affected by many parameters such as stoichiometry and stress, etc. Here we report that the growth modes, magnetism and transport properties of Fe3O4 thin films were strongly dependent on the oxygen pressure during film growth. The average roughness decreases from 1.021 to 0.263 nm for the oxygen pressure increase from $2.3{\times}10-7$ to $8.2{\times}10^{-6}$ Torr, respectively. The 120 K Verwey transition in Fe3O4 was disappeared for the sample grown under high oxygen pressure.

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Characteristic Changes of ITO/PET Thin Films with Ratio of Oxygen Partial Pressure (산소분압비에 따른 ITO/PET박막의 특성변화)

  • Kim, Hyun-Hoo;Lee, Moo-Young;Kim, Kwang-Tae;Yoon, Shang-Hyun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.05b
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    • pp.58-61
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    • 2003
  • ITO (indium tin oxide) thin films on PET (polyethylene terephthalate) substrate have been deposited by a dc reactive magnetron sputtering without heat treatments such as substrate heater and post heat treatment. Each sputtering parameter during the sputtering deposition is an important factor for the high quality of ITO thin films deposited on polymeric substrate. Particularly, the material, electrical and optical properties of as-deposited ITO oxide films are dominated by the ratio of oxygen partial pressure. As the experimental results, the excellent ITO films are prepared on PET substrate at the operating conditions as follows: operating pressure of 5 mTorr,target-substrate distance of 45 mm, dc power of 20-30 W, and oxygen gas ratio of 10 %. The optical transmittance is above 80 % at 550 nm, and the sheet resistance and resistivity of films are $24\;{\Omega}$/square and $1.5{\times}10^{-3}\;cm$, respectively.

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Effect of Ammonium Concentration on the Emission of $N_2O$ Under Oxygen-Limited Autotrophic Wastewater Nitrification

  • Kim, Dong-Jin;Kim, Yu-Ri
    • Journal of Microbiology and Biotechnology
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    • v.21 no.9
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    • pp.988-994
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    • 2011
  • A significant amount of nitrous oxide ($N_2O$), which is one of the serious greenhouse gases, is emitted from nitrification and denitrification of wastewater. Batch wastewater nitrifications with enriched nitrifiers were carried out under oxygen-limited condition with synthetic (without organic carbon) and real wastewater (with organic carbon) in order to find out the effect of ammonium concentration on $N_2O$ emission. Cumulated $N_2O$-N emission reached 3.0, 5.7, 6.2, and 13.5 mg from 0.4 l of the synthetic wastewater with 50, 100, 200, and 500 mg/l ${NH_4}^+$-N, respectively, and 1.0 mg from the real wastewater with 125 mg/l ${NH_4}^+$-N. The results indicate that $N_2O$ emission increased with ammonium concentration and the load. The ammonium removal rate and nitrite concentration also increased $N_2O$ emission. Comparative analysis of $N_2O$ emission from synthetic and real wastewaters revealed that wastewater nitrification under oxygen-limited condition emitted more $N_2O$ than that of heterotrophic denitrification. Summarizing the results, it can be concluded that denitrification by autotrophic nitrifiers contributes significantly to the $N_2O$ emission from wastewater nitrification.

Physiological Assessment of the Psychophysical Maximum Acceptable Weight of Load for Manual Materials Handling Tasks (인력물자취급시의 인체심리학적 최대허용중량의 생리학적 고찰)

  • Jeong, Seong-Hak;Kim, Hong-Gi
    • Journal of the Ergonomics Society of Korea
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    • v.18 no.1
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    • pp.1-12
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    • 1999
  • The objective of this study was to investigate the metabolic energy consumption rate of the psychophysical Maximum Acceptable Weight of Loads (MAWLs) for different manual materials handling tasks. Lifting activities with four different lifting frequencies (2, 5, 8, 11 lifts/min) for a lifting range (from floor to 76cm height) were studied. The oxygen consumption rate and heart rate were measured or recorded while subjects were lifting their MAWLs. It was found that the relationship between MAWL and frequency can be described best by the exponential function with the R-sq value 0.9865 for this study. Psychophysical MAWL decreased from 22.38 to 7.48 kg, while the oxygen consumption rate with the MAWL increased from 717.8 to $1114.7m{\ell}-O_2/min$ as the frequency increased from 2 to 11 lifts/min. Heart rate also increased from 104.5 to 120.7 bpm. The ratio of oxygen consumption for the MAWL to the Physical Work Capacity (PWC) ranged from 28 to 43%. The MAWLs were greater than or equal to the Maximum Permissible Limits (MPLs) when the frequencies were higher than 8 lifts/min. It seems that the MAWLs by psychophysical approach when the frequencies were higher than 8 lifts/min tend to be overestimated from the viewpoint of the physiological criterion of the oxygen consumption rates. From these findings it is suggested that the NIOSH Guideline should not be directly applied to Korean without reasonable modifications.

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SF6 and O2 Effects on PR Ashing in N2 Atmospheric Dielectric Barrier Discharge

  • Jeong, Soo-Yeon;Kim, Ji-Hun;Hwang, Yong-Seuk;Kim, Gon-Ho
    • Transactions on Electrical and Electronic Materials
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    • v.7 no.4
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    • pp.204-209
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    • 2006
  • Photo Resist (PR) ashing process was carried out with the atmospheric pressure- dielectric barrier discharge (ADBD) using $SF_6/N_2/O_2$. Ashing rate (AR) was sensitive to the mixing ratio of the oxygen and nitrogen of the blower type of ADBD asher. The maximum AR of 5000 A/min was achieved at 2% of oxygen in the $N_2$ plasma. With increasing the oxygen concentration to more than 2% in the $N_2$ plasma, the discharge becomes weak due to the high electron affinity of oxygen, resulting in the decrease of AR. When adding 0.5% of SF6 to $O_2/N_2$ mixed plasma, the PR AR increased drastically to 9000 A/min and the ashed surface of PR was smoother compared to the processed surface without $SF_6$. Carbon Fluorinated polymer may passivate the PR surface. It was also observed that the glass surface was not damaged by the fluorine.