• Title/Summary/Keyword: wise life

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Transcriptome Analysis of Bacillus subtilis by DNA Microarray Technique

  • Kang, Choong-Min;Yoshida, Ken-Ichi;Matsunaga, Masayuki;Kobayashi, Kazuo;Ueda, Minoru;Ogasawara, Naotake;Fujita, Yasutaro
    • Proceedings of the Korean Society of Life Science Conference
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    • 2000.06a
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    • pp.3-8
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    • 2000
  • The complete genome sequence of a Gram-positive bacterium .Bacillus subtilis has recently been reported and it is now clear that more than 50% of its ORFs have no known function (1). To study the global gene expression in B. subtilis at single gene resolution, we have tested the glass DNA microarrays in a step-wise fashion. As a preliminary experiment, we have created arrays of PCR products for 14 ORF whose transcription patterns have been well established through transcriptional mapping analysis. We measured changes in mRNA transcript levels between early exponential and stationary phase by hybridizing fluorescently labeled cDNA (with Cy3-UTP and Cy5-UTP) onto the array. We then compared the microarray data to confirm that the transcription patterns of these genes are well consistent with the known Northern analysis data. Since the preliminary test has been successful, we scaled up the experiments to ${\sim}$94% of the 4,100 annotated ORFs for the complete genome sequence of B. subtilis. Using this whole genomic microarray, we searched genes that are catabolite-repressive and those that are under the control of ${\sigma}^{Y}$, one of the functionally unknown ECF sigma factors. From these results, we here report that we have established DNA microarray techniques that are applicable for the whole genome of B. subtilis.

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A Study on clothing in Suhainmyuldo painted on an old tomb of ancient kingdom of Goguryeo between the $4^{th}$ and late $6^{th}$ century

  • Kang, Eun-Sook
    • Journal of Fashion Business
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    • v.10 no.3
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    • pp.54-68
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    • 2006
  • Suhainmyuldo (樹下人物圖) is a type of picture which expresses a person under the tree and is known to be brought to the Korean Peninsula from the Middle Asia. The origin of this kind of expression is assumed to be from Iykshini (or Iygsha), the fairy of tree, in India and the 'Tree of Life' in Western Asia which influenced on the craft art design of Chinese art. However, Chinese art had already developed the motif of this kind in its unique way; for example, Jookrimchilhundo (竹林七賢圖: seven wise men in bamboo forest) in the Six Dynasty. There are only two items of female dress and its ornament from the 4th to 6th century Suhainmyuldo of the Goguryeo ancient tomb mural: one from the Jangchun Tomb No. 1 in which a female figure is portrayed as a part of living genre painting and the other from the Anahk Tomb No. 1 with seven female figures in a painting which is one of the earliest Suhainmyuldos, presumably from the 4th century. These two paintings must be considered in parallel with the origin of Suhainmyuldo. The mountain wizard's dress and Men's dress and its ornament in Suhainmyuldo from the 4th to the late 6th century Goguryeo ancient tomb is mostly joint adjust in which its collar is connected by a line and almost all the wizards are wearing Sang (upper garment). Most mountain wizards are wearing black feather shaped jackets and upper garments. One noticeable observation is that the mountain wizards of Mooyongchong, Tongu Ohgoibun Nos. 4 and 5 are completely different from one another in terms of ways to dress each wizard's official hats and shoes.

Vocabulary Education for Korean Beginner Level Using PWIM (PWIM 활용 한국어 초급 어휘교육)

  • Cheng, Yeun sook;Lee, Byung woon
    • Journal of Korean language education
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    • v.29 no.3
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    • pp.325-344
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    • 2018
  • The purpose of this study is to summarize PWIM (Picture Words Inductive Model) which is one of learner-centered vocabulary teaching-learning models, and suggest ways to implement them in Korean language education. The pictures that are used in the Korean language education field help visualize the specific shape, color, and texture of the vocabulary that is the learning target; thus, helping beginner learners to recognize the meaning of the sound. Visual material stimulates the intrinsic schema of the learner and not only becomes a 'bridge' connecting the mother tongue and the Korean language, but also reduces difficulty in learning a foreign language because of the ambiguity between meaning and sound in Korean and all languages. PWIM shows commonality with existing learning methods in that it uses visual materials. However, in the past, the teacher-centered learning method has only imitated the teacher because the teacher showed a piece-wise, out-of-life photograph and taught the word. PWIM is a learner-centered learning method that stimulates learners to find vocabulary on their own by presenting visual information reflecting the context. In this paper, PWIM is more suitable for beginner learners who are learning specific concrete vocabulary such as personal identity (mainly objects), residence and environment, daily life, shopping, health, climate, and traffic. The purpose of this study was to develop a method of using PWIM suitable for Korean language learners and teaching procedures. The researchers rearranged the previous research into three steps: brainstorming and word organization, generalization of semantic and morphological rules of extracted words, and application of words. In the case of PWIM, you can go through all three steps at once. Otherwise, it is possible to divide the three steps of PWIM and teach at different times. It is expected that teachers and learners using the PWIM teaching-learning method, which uses realistic visual materials, will enable making an effective class together.

A study on the implementation of door control unit for railway trains operable at low and high platforms

  • Young-Seok Cho
    • Journal of the Korea Society of Computer and Information
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    • v.28 no.7
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    • pp.1-9
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    • 2023
  • Currently, as the demand for stops in the urban increases resulting from an increase in the supply of express trains, the development of trains capable of operating both high-floor platforms in the urban and low-floor platforms in the suburbs is required. In this paper, we studied the design and fabrication of a controller for train doors that consists of low and high steps as a plug-in type door mechanism and thus can be used on low and high platforms. This DCU H/W was designed and implemented using 32 bit MCU to control 4 motors, 33 digital inputs and 16 digital outputs. In addition, based on the software life cycle of EN50128, 2 items of operation requirements and 12 items of control requirements were derived, and then they were designed and implemented as operation SW. The implemented SW was tested for each requirement. As a result, we performed tests on 13 items that could be tested in the mock-up out of 14 total requirement items and showed that the requirements were satisfied.

The Effects of Career Orientation on the Entrepreneurial·Reemployment Intention: Focused on the Mediating Effects of the Social Capitals (경력지향성이 창·재취업의도에 미치는 영향 연구: 사회적자본의 매개효과를 중심으로)

  • Kang, Gyung Lan;Park, Cheol Woo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.12 no.5
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    • pp.177-189
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    • 2017
  • The purpose of this research is to investigate the factors which effect the intention to reemploy after the retirement and the mediation effect of the social capitalism for retired and prospective retired baby boomers. Starting from 2010, baby boomers have been retiring at a large scale. However retirement at a large scale without securing provision for fiancial wise in later life can lead to many issues regarding social and fiancial wise such as instability of labour market and increase of spending in society welfare cost and such on. Change of individual's self prospective and institutional support to help prepare find new career for next 3decade after the retirement needs to be concretized. The results of this study suggest that the management orientation affects the social capital positively and the autonomous orientation does not affect the relationship between career orientation and social capital. In terms of the relationship between career orientation and reemployment intention, management orientation does not affect the intention of the entrepreneur but affects the reemployment intention positively, while the autonomous intention affects neither entrepreneurial intention nor reemployment intention (+), Respectively. Social capital has a positive effect on the intention of reemployment, and the mediating effect of social capital on the relationship between career orientation and reemployment. Also, while in the work force, potential retiring baby boomer workers should be systematically supported with education, training and reemployment program based on individual's career orientation and allowing them to stay for a longer period in the labour market can be the implication to the solution for the economic stability and increasing social cost such as public pensions.

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Priority Analysis of Project Stage-wise Risk Factors : Focusing on New Product Development Projects in ICT Industry (프로젝트 단계별 리스크 요인들의 우선순위 분석 : ICT(정보통신기술)산업 분야의 신제품 개발 프로젝트를 중심으로)

  • Jang, Heeseok;Choi, Sungyong;Lee, Minho
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.41 no.3
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    • pp.72-82
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    • 2018
  • In this paper, we identify risk factors that are likely to occur during the lifecycle of a new product development (NPD) project from the literatures, and identify the three objectives or three constraints that will ultimately be achieved for project success in the ICT industry : performance (scope/quality), schedule (time), and cost. Firstly, we interviewed the project experts to classify the risk factors according that the final project objectives are changeable based on scope/quality, time and cost budget constraints. Secondly, the survey for pairwise comparisons between the risk factors was asked to the project managers and members who had ever actually participated in the NPD projects of ICT industry to determine the priority ranks on relative importance using AHP (Analytic Hierarchy Process). The risk factors negatively affecting the goals of projects were analyzed by using the AHP respectively in four project stages during the life cycle of the project. The comparison of risk factors within each stage is a different approach unlike the literatures which have covered project's overall risk assessment. There is an advantage that risk management can be effectively performed with priorities according to each stage from the start to the end of the project. In other words, it is necessary to identify what risk factors will occur in each stage, and to have ideas at each stage with the priorities so that they can be mitigated and eliminated before actual occurrence. As a result, risks on scope & quality changes were found to be the most important considerations for initiative stage of NPD projects in the ICT industry, whereas in the final stage, risks on schedule (time) changes were the most important priorities. Among the ICT industry product categories, 'communication and broadcasting devices' and 'IT and communication based devices' generally have a high priority in terms of risks on scope & quality changes when initiating the project. At the closing stage of the project, however, considering that schedule (time) changeable risk is getting higher, these products tend to target at B2B market rather than B2C because the new products must be delivered and launched in time as customer firm required.

Post-evaluation of the Seoul Garden Show through a Survey (설문조사를 통한 서울정원박람회의 사후평가)

  • LEE, Hyukjae;Hong, Seunghun
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.5
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    • pp.741-747
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    • 2022
  • The purpose of this study is to analyze the success and failure factors of the Seoul Garden show to redefine the purpose and goal of the Seoul Garden show in the future, and to create a foundation for the Seoul Metropolitan Government to contribute to the popularization and spread of garden culture and improvement of emotional life. In order to achieve the above purpose, a survey was conducted on two groups of the general public and exhibition participants. The contents of the survey include identifying satisfaction factors and problems with the Seoul Garden show, and establishing the direction of the Seoul Garden show. As for the factors of success, the number of writers was the highest, and the improvements were overall for each element of the Seoul Garden show, but mainly for industrial exhibitions, rest areas, and publicity. In addition, the Seoul Garden show should be held in the old park with the aim of spreading, popularizing, and living the garden culture, and many gardens should be created around the artist's garden. The Seoul Garden show is contributing to society in many ways, and there are some improvements, but considering the high willingness to revisit the Seoul Garden show, it can be said that the Seoul Garden show so far is valuable enough.

Genetic Diversity in Three Populations of Hibiscus hamabo(Malvaceae) in Jeju Island, Korea (제주도 황근(Hibiscus hamabo) 집단의 유전적 다양성)

  • Kim, Young-Dong;Kim, Ki-Joong;Kim, Sung-Hee;Kim, Hyeong-Tae
    • Korean Journal of Plant Taxonomy
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    • v.37 no.2
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    • pp.115-129
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    • 2007
  • Using internal transcribed spacer (ITS) sequences and inter-simple sequence repeats (ISSRs) data, genetic diversity of a rare species, Hibiscus hamabo Siebold & Zucc. was examined for 3 populations in Jeju Island, Korea. A total of 14 nucleotide (excluding 3 ambiguous nucleotide) site variation in the ITS was observed from 18 individuals (Population 1, Hadori), which differed up to 13 bp in pair-wise comparison. On the contrary, the ITS sequences of all individuals in Populations 2 and 3 were identical. Genetic diversity estimates including Nei's gene diversity (h) generated by ISSR data were substantially high in Population 1 compared to other two populations. Low genetic variation in Populations 1 and 2 is considered due to genetic drift (bottleneck effect) and limited gene flow in these populations. Considering the differences in genetic diversity, protection of the Population 1(Hadori) is very critical for in situ conservation of Hibiscus hamabo in Korea. If ex situ conservation is required, making the full use of Population 1 will be most efficient.

Elementary Science Textbook Analysis of Korea and the United States (한국과 미국의 초등학교 과학 교과서 분석)

  • Kim, Hyo-Nam;Park, Do-Yong
    • Journal of Science Education
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    • v.33 no.2
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    • pp.258-270
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    • 2009
  • Science textbook is the most frequently used teaching material in elementary schools of the United States and Korea. Elementary science textbooks of the United States and Korea are analyzed to find out the educational objectives and characteristics of contents shown in textbooks. About 100 pages each in the first grade and fourth grade science textbooks each nations are selected randomly for educational objective analysis. Life science contents of 1st to 6th grade are analyzed from elementary science textbooks of the United States and Korea. The analyzed textbooks in Korea are 'Wise life,' an integrated subject with social studies and science, and 'science.' The analyzed elementary science textbooks of the United States are Harcourt Science, which is one of the frequently used textbooks. The educational objective framework used includes science knowledge, scientific inquiry, scientific attitude, STS, and philosophy and history of science. The results show science textbooks of the United States emphasize scientific knowledge more than scientific inquiry. Korean science textbooks emphasize scientific inquiry more than scientific knowledge. Elementary science textbooks of the United States present some life science topics redundantly and expose more difficult topics than Korean.

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A Study on the Improvement of the Safety and Safe Living Standards of Apartment Houses through AHP Analysis (AHP 분석을 통한 공동주택 안전 및 안심생활 수준 향상에 관한 연구)

  • Ji, Yung il;Shin, Seung Ha;Choi, Byong Jeong
    • Journal of the Society of Disaster Information
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    • v.17 no.2
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    • pp.289-305
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    • 2021
  • Purpose: The purpose of this study is to check the importance of higher level, sub higher level and lower alternative factor and the factor for relieved life and also check the comprehensive priority in executed "Safety and relieved life level improvement of apartment house residents by AHP analysis". Method: The safety management evaluation elements and alternatives have been arranged as hierarchical structure based on the preceding studies and the advice of experts for the maintenance and system of apartment house in this study. Study model is used for the survey of pair wise comparison composition targeting experts and AHP analysis method is used for alternative plan ranking, etc. Result: The response result of survey secured the consistency and it is confirmed that safety inspection for apartment house electricity facility is most important for higher level, safety inspection, detailed inspection and diagnosis evaluation are most important for sub higher level and plumbing equipments is most important for alternatives based on the analysis result of relative weight comparison for sub higher level and alternatives for final goal attainment of the study. Conclusion: Plumbing equipments, crime prevention safety and electrical safety are shown as top priority alternatives when observe it with expert manager's point of view for apartment house. It shows that securing the importance of living safety accident and deciding the level of relieved life can be improved are the alternatives of pre-prevention and management strengthening.