• Journal of fish pathology
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• v.30 no.2
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• pp.71-78
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• 2017

The glycoprotein of novirhabdoviruses is known to play a critical role in the determination of host specificity. Viral hemorrhagic septicemia viruses (VHSVs) in different genotypes have different glycoprotein sequences and show different preferences for specific cell lines. In this study, to know whether the glycoprotein is solely responsible for the host cell preference of VHSV, a recombinant VHSV expressing vesicular stomatitis virus (VSV) glycoprotein instead of VHSV IVa glycoprotein (rVHSV-VSV-G) was generated by reverse genetics and inoculated into several fish cell lines, then, cytopathic effect (CPE) and viral growth caused by rVHSV-VSV-G infection were compared with those caused by rVHSV-wild that was previously generated and has the same genomic sequence with wild-type VHSV except a few nucleotides. The plaque numbers of rVHSV-VSV-G were significantly higher in EPC, BF-2 and GF cells than those of rVHSV-wild. However, in HINAE cells (originated from olive flounder), rVHSV-VSV-G titer was significantly lower than rVHSV-wild titer, and both recombinant VHSVs were not grown well in CHSE-214 cells. Although statistical significances were detected in the titers between rVHSV-wild and rVHSV-VSV-G in several cell lines, the cell line-preference order of rVHSV-VSV-G was not different from that of rVHSV-wild. These results suggest that the replacement of VHSV glycoprotein may not completely change host cell preference, and other regions of VHSV might also involve in the determination of host cell preference.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.92-92
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• 2005

• Fisheries and Aquatic Sciences
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• v.26 no.9
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• pp.569-581
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• 2023

The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

• Journal of fish pathology
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• v.22 no.1
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• pp.75-83
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• 2009

Disease surveillance was performed to monitor the prevalence of fish pathogens in wild marine fish caught in coastal offshore water from February to October in 2008. A total of 401 fish samples were collected at set net or fish market at landing port on the coast of Pohang, Geoje, Yeosu and Jeju. In this study, 17 kinds of fish pathogens were isolated from 152 fish samples. The detection rates of parasites, bacteria or viruses were 21.4%, 17.0% and 2.7%, respectively. The detected parasites were Scutica, Trichodina, Cryptocaryon, Dactylogyrus, Microcotyle, Benedenia, Bivagina, Heteraxin, Caligus, Epistylis and nematode. The dominant bacterial pathogens were Vibrio, Streptococcus, Photobacterium and Psuedomonas. Red sea bream iridovirus (RSIV) and lymphocystis disease virus (LDV) were detected in 6 species of fish virus examined in this study. The detection rates of fish pathogens from Scorpaenidae, Monacanthidae, Pleuronectidae, Sparidae and Carangidae investigated over 30 samples were 59.2%, 48.4%, 34.2%, 30.6% and18.2%, respectively.

• Journal of Aquaculture
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• v.7 no.2
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• pp.109-122
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• 1994

The cultured olive flounder larvae given nutrient enriched rotifer, Artemia nauplii and commertial micro diet were investigated the changes of lipid content, fatty acid composition and vitamin E content in whole body with growth stages in order to compare with those of the wild one. The results obtained are as follows. 1) Lipid and vitamin E contents of the cultured larvae were higher than those of the wild one. 2) Clear differences of fatty acid composition with growth stages, especially 18: 2n-6 and 22 : 6n-3, were recognized between the wild and cultured olive flounder larvae. 3) Changes of lipid content, fatty acid composition and vitamin E content were found to be affected by dietary composition given to fish. 4) The wild olive flounder larvae were found to obtain natural live foods containing of low lipid content and high concentration 22: 6n-3 based on the fatty acid composition of the fish.

• Development and Reproduction
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• v.24 no.4
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• pp.317-325
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• 2020

The Far Eastern catfish (Silurus asotus) is an important commercial freshwater fish in Korea. Investigation of the genetic diversity of wild and cultured domestic catfish groups is essential for the restoration of fishery resources and for increasing local revenue. However, there are relatively few genetic diversity studies on wild and cultured catfish in Korea. In the present study, we analyzed the genetic diversity and association of wild and cultured catfish using five microsatellite markers. We determined that the number of alleles per locus (N_A) ranged from 9 to 25, wherein the Jeonbuk catfish demonstrated the highest mean number of alleles per locus and the cultured catfish exhibited the lowest. The average expected heterozygosity (He) of the wild catfish samples was 0.907, and that of the cultured catfish showed was 0.875. The genetic distances (GD value) among populations of all catfish ranged from 0.138 to 0.242. Jeonnam and Jeonbuk wild catfish were located closest to each other, and the cultured group was separated from the other groups. In conclusion, the present study confirmed that the genetic diversity of wild and cultured catfish was maintained at a high level. In the case of the wild group, it is effective in maintaining diversity due to the continuous fry release by the local fish research institute. However, the genetic diversity of cultured catfish declined. Low diversity is associated with slow growth and weakened immunity, and therefore continuous monitoring is necessary.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.248-253
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• 2018

Freshwater farms are primarily located adjacent to rivers and lakes, facilitating the introduction and spread of pathogens into natural systems. Therefore, it is necessary to continuously monitor natural aquatic organisms, the breeding environment, and infection rates by pathogenic organisms. Fish and crustaceans were sampled 4 times in the Geum River estuary in 2016. The samples were analyzed for the presence of pathogens for reportable communicable diseases, including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease); parasite abundance was also examined. The dominant fish species were deep body bitterling Acanthorhodes macropterus (21.4%), followed by skygager Erythroculter erythropterus (12.7%). For crustaceans, Palaemon paucidens and Chinese mitten crab Eriocheir sinensis were dominant. Sixty fish and 36 crustacean species were examined for reportable communicable diseases. When using a specific primer set for each disease, PCR analysis did not detect any reportable communicable diseases in the samples. Some instances of Dactylogyrus, copepods, nematodes and metacercaria were detected. However, the PCR results indicated that the metacercaria were not Clonorchis sinensis.

• Journal of fish pathology
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• v.13 no.1
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• pp.15-20
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• 2000

Infection rates with white spot baculovirus(WSBV) in wild-caught shrimp spawners appeared to be 52% in Penaeus chinensis and 20% in Penaeus japonicus when diagnosed by polymerase chain reaction (PCR). Infection rates of WSBV from inhabitant organisms in shrimp farm showed 37% in Upogebia major and 29% in Calliamassa japonica. The viruses maintained their survivorship in seawater at $4^{\circ}C$, but lost it at $25^{\circ}C$.

• Journal of fish pathology
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• v.26 no.3
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• pp.129-137
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• 2013

The study surveyed infection rate of Kudoa septempunctata parasitized in the trunk muscle of olive flounder, Paralichthys olivaceus, cultured in Jeju Island and wild fish species caught in the coastal area around Jeju Island during 2012. Among 143 olive flounder that were randomly sampled from 26 different culture farms, K. septempunctata was detected in 7 fish samples (4.9%) from 4 different culture farms, showing no typical Kudoa infestation. However, K. septempunctata was not detected in olive flounder fry sampled from hatcheries and 8 species of wild fish. In addition, we compared 3 different sampling sites on trunk muscle of 7 Kudoa positive fish that included head part, tail part and entire muscle. Among 7 fish, K. septempunctata was detected in 3 fish that were sampled from head part; while 4 fish from tail part of trunk muscle. However, all 7 fish were positive when sampled from entire muscle. Thus, we suggest that it will be more efficient to use entire muscle sample than sampling partial muscle parts for detection of K. septempunctata.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.6
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• pp.462-468
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• 2004

In order to evaluate adaptation to the environment in released fish, digestive organ and body composition were compared among 1-year old cultured (CUL), released (REL) and wild (WIL) Black Sea Bream, Acanthopagrus schlegeli. Hepatosomatic index (HSI) of the REL was significantly lower than those of the CUL and WIL fish, but intestine weight index (IWI) of REL was significantly higher than those of the CUL and WIL fish. There were no significant differences in stomach weight index (SWI) and condition facto. (CF) among those different fish groups. The number of pyloric caeca of the REL and WIL fish were 4, but the $30{\%}$ of CUL fish was 3 counts. The moisture content of whole body in the WIL fish was significantly higher than the CUL and REL fish, but significant lowest in the crude lipid of whole body. The content of moisture and crude protein of dorsal muscle were no significant differences among the CUL, REL and WIL fish, but crude lipid content of WIL fish was significantly lower than the CUL and REL fish. Moisture content of CUL fish in the liver was significantly lower than the REL and WIL fish, and crude lipid was significant lowest in the WIL fish. Amino acid content of dorsal muscle in the WIL fish was highest in the total amino acid, EAA and E/A ratio, and CUL fish was highest EAA and E/A ratio in liver. EPA content in dorsal muscle of WIL fish was lower than CUL and REL fish, and the REL fish was highest in DHA content of the fatty acid. EPA content in liver of WIL fish was lower than CUL and REL fish, and DHA content in REL and WIL fish was highest and lowest. Crude protein content of CUL and WIL fish in the scale was significantly higher than REL fish, but there were no significant differences in contents of crude lipid and ash. Ca and P of scale were formed most of mineral and content of P in CUL fish was significantly higher than those of REL and WIL fish. There were no significant differences in Mg, K, Na and S of scale, but CUL fish was significantly lower than REL fish in C1. The results suggest that difference of digestive organs and body composition concluded it from differences of inhabitation environment and feed formulations.