• Journal of Embryo Transfer
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• v.28 no.3
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• pp.215-222
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• 2013

Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of $E^D$, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of $E^+$ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, $E^+/E^+$ (sire 1), $E^+/e$ (sire 2 and dam 3), $E^+/e$ with 4 bands of 174, 207 and 328 bp (dam 1) and $E^+/e$ with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), $E^+/E^+$ (22%) and $E^+/e$ (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, $E^+/E^+$ with 67% and $E^+/e$ with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had $E^+/e$ genotype and the dam had $E^+/E^+$ with the 3 bands or $E^+/e$ genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires ($E^+/e$) and dams ($E^+/E^+$ with the 3 bands or $E^+/e$) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.181-188
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• 1991

Epidemiological studies on host rodents of tsutsugamushi disease were carried out during the period of July∼September 1990 at nine localities of central Korea. Among total 111 wild rodents trapped by the modified Sherman live traps, 103 were Apodemus agrarius (92.8%), seven were Crocidura lasiura (6.3%) and one was Microtus fortis (0.9%) , showing 24.0% of trapping rate in winter, 11.7% in spring, 11,2% in summer and 12.0% in autumn. Out of 103 A. agrarius 84 were parasitized by chiggers, showing 81.6% of the infestation rate and 43.0 of the chigger index. The antibody positive rate of A. agrarius sera to Rickettsia tsutsugamushi was significantly variable by locality, being in the range of 0∼78.6%. The seasonal change of the antibody positive rate at Dorai 5-ri, Goyang-gun was 75.8% in average during November∼March, decreased to 30.3% in April and further decreased to 13.3% in average during May∼August. Among 33 antibody positives, 31 were Karp strain and two were Gilliam. Seven Crocidura lasiura sera showed all negative. R. tsutsugamushi organisms were isolated from three A. ngrarius out of 94 mice tested, showing 3.2% of the infection rate.

• Journal of the Korean Data and Information Science Society
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• v.25 no.2
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• pp.271-280
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• 2014

Foot-and-mouth Disease (FMD) is a highly infectious and fatal viral livestock disease that affects cloven-hoofed animals domestic and wild and the FMD outbreak in Korea in 2010/2011 was a disastrous incident for the country and the economy. Thus, efforts at the national level are put to prevent foot-and-mouth disease and to reduce the damage in the case of outbreak. As one of these efforts, it is useful to study the spread of the disease by using probabilistic model. In fact, after the FMD epidemic in the UK occurred in 2001, many studies have been carried on the spread of the disease using a variety of stochastic models as an effort to prepare future outbreak of FMD. However, for the FMD outbreak in Korea occurred in 2010/2011, there are few study by utilizing probabilistic model. This paper assumes a stochastic spatial-temporal susceptible-infectious-removed (SIR) epidemic model for the 2010/2011 FMD outbreak to understand spread of the disease. Since data on infections of FMD disease during 2010/2011 outbreak of Aniaml and Plant Quarantine Agency and on the livestock farms from the nationwide census in 2011 of Statistics Korea do not have detail informations on address or missing values, we generate detail information on address by randomly allocating farms within corresponding Si/Gun area. The kernel function is estimated using the infection data and by using simulations, the susceptibility and transmission of the spatial-temporal stochastic SIR models are determined.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.6
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• pp.35-46
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• 2012

This study attempts to specifically analyze green volume and scenery characteristics by classifying the streets in Seoul and suggest improvements accordingly. The subject streets for research were limited to those that have high occupancy ratio and are wide enough to have a big potential in improving green volume. In terms of green volume and scenery according to the types of streets, Platanus occidentalis and Ginkgo biloba were most common regardless of the street type, and the green ratio was 51.6% in residential areas, 50.4% in commercial areas, and 43.7% in business areas. Apart from the residential area, there was almost no green areas, and the ratio of green coverage was 71.0% in business areas, 64.0% in green areas, 37.3% in residential areas, and 36.2% in commercial areas, while the green volume coefficient was $1.9m^3/m^2$ on average. Based on the study results, it was set as a goal to provide the residential areas with a green community space and flowering trees throughout the year for scenery, and commercial areas with flowering trees and maples to emphasize the stores' image while not interfering with service and walking. For business areas, the goals were to improve green ratio and create streets that suit the urban image, and to set up spaces to provide habitat for wild animals with multi-layer planting and link to surrounding forests.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.10
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• pp.7176-7181
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• 2015

This thesis is about the system where the solar module is attached to the high functional guardrail posts with anti-weed, anti-plant, and road-kill applied to produce internal power, enabling the integrated control and real-time monitoring of appearance of wildlife and road conditions using the USN. The whole system consists of a photovoltaic module(PV), a detection sensor(pyroelectric), a controller(operation select and motion sensor), the USN system, the DB(sound and flash), an output unit of sound and flash, and the control system of road-kill prevention and safety induction for vehicles. Thus this study aims to address the remote control and monitoring system of multipurpose guardrails to improve road environment, prevent road-kills, protect wild animals, and guide cars safely by using the USN which is combined with new renewable energy and IT convergence technology. As a result of the study on the remote control and monitoring system using the USN, it was ascertained that the response time of the unmanned sensing system was within 5.1 ms with the current consumption of 0.328 mA, and the data transmission speed of the remote control system was 250 kbps with the current consumption of 0.283 mA.

• Journal of fish pathology
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• v.22 no.3
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• pp.201-210
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• 2009

Although Anisakis type larvae have been shown to cause various injuries directly or indirectly in humans and animals, the epidemiological studies on these larval infestations are in insufficient state. The status of larval infestation was investigated in 989 fishes of 44 species, which are inhabiting around the east-westernsouth costal area of Korea during the period from March 2007 to February 2008. The Anisakis type larvae were infected approximately 38% (377 fishes) in 989 fishes. Most of the worms were identified as Anisakis simplex type I by morphological finding and 18S ribosomal DNA sequence analysis. In the seasonal variations of infestation, most of the fishes showed higher infestation rate during spring and summer, while the fishes such as herring Clupea pallasii did during winter. From the histopathological studies of infested fishes, it has been observed that Anisakis type larvae are harbouring mainly around the intestinal viscera such as liver, pancreas, stomach, pylolic cecum, and cloaca.

• Korean Journal of Ecology and Environment
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• v.50 no.3
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• pp.314-324
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• 2017

In order to consider application and evaluation of value and class of domestic wetland, we investigated 146 wetlands located Gyeongsangnam-do using Rapid Assessment Method (RAM). We utilized Self-Organizing-Map (SOM) to analysis relationship between evaluation index and land coverage ratio surrounding wetland. Among total 8 evaluation index, 'Fish and herptile habitat' and 'Aesthetic value' were higher, most of the wetlands evaluated as 2, 3 grade. Result of SOM analysis, 'vegetation diversity and wild animals habitat' is negatively related to the 'Fish and herptile habitat', because fishs were not prefer habitat excessively occupied by plant. However, high vegetation diversity can be support high score of 'Aesthetic' in wetland. Also, 'Erosion control' and 'Flood storage and control' were closely related, wetlands with high score of 'Erosion control' have high score of 'Flood storage and control'. When applied RAM in domestic wetland, six out of 6 evaluation index induced biased results, the index of RAM need a little change as some new or modify evaluation index. Therefore, we consider to need adjustable, subdivide, and actualization of some evaluation index for application of RAM in domestic wetlands. Consequently, wetland assessment and class using RAM can be utilized as important indicate for conservation and management of wetland, and contributed greatly to maintain biodiversity include to endangered species by preserving remaining wetland.

• Food Science of Animal Resources
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• v.23 no.4
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• pp.309-314
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• 2003

Color and its stability in venison, longissumus dorsi (LD) and quadriceps femoris (QF) muscles, from 8 wild Cervus nippon yesoensis (Japanese Yeso Deer) were investigated by means of the CIE L$^{*}a{*}b{*}$ measurement and autoxidation rate recorded using partially purified myoglobin. It was observed a common feature of the change of three mean values($L^{*}$, $a^{*}$ and $b^{*}$) in both LD and QF that mean value increased at 1 or 2 day post-mortem and then decreased during storage. The differences between 1 and 7 days was the largest in $a^{*}$ value than those in $L^{*}$ and $b^{*}$ values. The mean differences among storage days were only significant in $a^{*}$ except for $b^{*}$ of LD. It was same tendency that the mean difference of CIE $L^{*}$, $a^{*}$ and $b^{*}$ values during refrigerator storage was larger in $a^{*}$ than both in $L^{*}$ and $b^{*}$ reported in beef(Sekikawa et al., 1995) and venison(Stevenson et al., 1989) during storage. The smaller $a^{*}$ value was indicated that bright red of meat changed to dull red, brown red causing met-Mb formation. To compare of color stability with respects to the Mb autoxidation rate, we measured this rate of deer and horse muscles, because horse Mb was considered to have the fastest autoxidation rate among domestic animals, and we used crude Mb and pH 6.0, which might be reflected to the intact meat. Mean value of the autoxidation rate measured in this study in deer was 0.037 and that was 0.026 in horse(sigma). Although there was no significant mean difference and were different Mb purity between deer(A409/A 280 nm = 4.0) and horse(5.6), in generally Mb purity was the higher and the faster autoxidation rate, but this rate in deer was faster than in horse. These results might indicate that venison meat discolors at faster rate compared with beef.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.13-19
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• 2012

To determine the status of Korean molluscs infection and relation with climate change of Korean peninsula, references were reviewed. Wild animals serve as intermediate, reservoirs and paratenic hosts to zoonotic parasites of human beings. Trematode are common parasites of molluscs and almost all trematodes infect mollusks as the first host in the life cycle, and most have a complex life cycle involving other hosts. The significance of auxiliary hosts to the ecology of the parasite has not been proper discussion. There is increasing concern as to the impact of climate change on the epidermiology of many parasitic diseases. A total of 21 species for snail transmitted parasites from Korean molluscs has been reviewed. Among them, 15 species was aquatic mollusks and 6 species for marine mollusks. Maximum infections belonged to Semisulcospira livertina had 11 kind of parasite pathogenic organisms, including Paragonimus westermani, Metagonimus yokogawai, Centrocestus armatus, Notocotylus magniovatus, Centrocestus formosanus, incerte cercaria, nipponensis cercaria, Yoshidae cercaria, cristata cercaria, innominatum cercaria and Metagonimus sp. And 11 in Parafossarulus manchouricus including Clonorchis sinensis, Asymphylodora japonica, Cyathocotyle orientalis, Exorchis oviformis, Notocotylus attenuatus, Echinochasmus japonicus, Loxogenes liberum, Cercariae of Loxogenes liberum Type I, Cercariae of Loxogenes liberum Type II, Furcocercus cercariae (Family Sanguincolidae) and Cercaria of Mucobucaris, 10 in Semisulcospira sp. including Paragonimus westermani, Metagonimus yokogawai, Centrocestus armatus, Echinochasmus redioduplicatus, Notocotylus magniovatus, Cercaria incerte, Cercaria nipponensis, Cercaria yoshidae, paludinarum cercaria and Metagonimus sp., 7 in Koreanomelania globus including Pseudexorchis major, Cercaria of parapleurolophocercous type, Metagonimus sp. (A & B), Cercaria nipponensis, Cercaria inserta and Cercaria yoshidae. Also, Tapes philippinarum have 3 pathogenic organisms including Cercariae tapidis, Cercariae furcocercus and Parvatrema sp. In particular, under climatic extremes such as floods and drought, aquatic molluscs may play a more prominent role in parasite transmission in the future.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1446-1451
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• 2016

Clostridium difficile toxin A causes acute gut inflammation in animals and humans. It is known to downregulate the tight junctions between colonic epithelial cells, allowing luminal contents to access body tissues and trigger acute immune responses. However, it is not yet known whether this loss of the barrier function is a critical factor in the progression of toxin A-induced pseudomembranous colitis. We previously showed that NADH:quinone oxidoreductase 1 (NQO1) KO (knockout) mice spontaneously display weak gut inflammation and a marked loss of colonic epithelial tight junctions. Moreover, NQO1 KO mice exhibited highly increased inflammatory responses compared with NQO1 WT (wild-type) control mice when subjected to DSS-induced experimental colitis. Here, we tested whether toxin A could also trigger more severe inflammatory responses in NQO1 KO mice compared with NQO1 WT mice. Indeed, our results show that C. difficile toxin A-mediated enteritis is significantly enhanced in NQO1 KO mice compared with NQO1 WT mice. The levels of fluid secretion, villus disruption, and epithelial cell apoptosis were also higher in toxin A-treated NQO1 KO mice compared with WT mice. The previous and present results collectively show that NQO1 is involved in the formation of tight junctions in the small intestine, and that defects in NQO1 enhance C. difficile toxin A-induced acute inflammatory responses, presumably via the loss of epithelial cell tight junctions.