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A STUDY OF EFFECTS OF BONE MORPHOGENETIC PROTEIN BONE REGENERATION OF IMPLANTS IN DOGS (성견에서 임프란트 매식시 골형성단백 사용에 따른 골재생에 관한 연구)

  • Jo Jin-Hee;Vang Mong-Sook;Lee Jong-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.32 no.4
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    • pp.593-607
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    • 1994
  • The purpose of this study is to evaluate the effect of the bone morphogenetic protein, bone matrix gelatin and collagen matrix on the amount and shape of generating new bone adjacent to the implant. Implants were inserted in the mandible of adult dogs at 2 months after teeth extraction. Artificial bony defects, 3mm in width and 4mm in depth were made at the mesial and distal side of implant. Experimental groups were divided into three groups ; Group 1 : Defects filled with collagen matrix and bone morphogenetic protein, Group 2 : Defects filled with bone matrix gelatin. Control group : Defects filled with only collagen matrix. After implantation, the animals were sacrificed at 1,3,5 and 10 weeks for light microscopic examination. For the fluorescent microscopic examination. each tertracycline Hcl and calcein were injected at 1, 3, 5, 8 and 10 weeks after implantation. The results obtained were as follows : 1. The molecular weight of bovine BMP was about 18,100 by hydroxyapatite chromatography. 2. Osseointegration was observed in experimental groups 1 & 2, and BMG and BMP had an excellent bone forming capability as a filling materials to the repair of the bone defects. 3. The degree of healing of bone defect area, the experimental group 1 showed more prominent bone formation than control group, and the control group showed fibrous connective tissue between the implant and the bone. 4. In the fluorescent microscopic findings, bone remodelling was observed regenerative lamellar bone at defect area in experimental group 1, and partial remodelling in experimental group 2, In the control group, fibrous connective tissue was observed between the implant and bone surface and sign of remodelling was not apperaed. Above results suggest that BMP has rapid osteoinductive property and can be used clinically as a bone substitute on bone defects around implants.

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Development of LED Irradiation System for Cell Proliferation (세포증식을 위한 LED 조사 시스템 개발)

  • Cheon, Min-Woo;Park, Yong-Pil;Lee, Ho-Shik;Kim, Tae-Gon;Kim, Young-Pyo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.05a
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    • pp.581-582
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    • 2010
  • This paper performed the basic study for developing the Photodynamic Therapy Equipment for medical treatment. We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Control stage is divided into 30 step by program. Consequently, the current value could be controlled by the change of level in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage.

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License Plate Location Using SVM (SVM을 이용한 차량 번호판 위치 추출)

  • Hong, Seok-Keun;Chun, Joo-Kwong;An, Myoung-Seok;Shim, Jun-Hwan;Cho, Seok-Je
    • Journal of Navigation and Port Research
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    • v.32 no.10
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    • pp.845-850
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    • 2008
  • In this paper, we propose a license plate locating algorithm by using SVM. Tipically, the features regarding license plate format include height-to-width ratio, color, and spatial frequency. The method is dived into three steps which are image acquisition, detecting license plate candidate regions, verifying the license plate accurately. In the course of detecting license plate candidate regions, color filtering and edge detecting are performed to detect candidate regions, and then verify candidate region using Support Vector Machines(SVM) with DCT coefficients of candidates. It is possible to perform reliable license plate location bemuse we can protect false detection through these verification process. We validate our approach with experimental results.

The Effects of Kimchi on Hematological and Immunological Parameters in vivo and in vitro (In vivo와 in vitro에서 김치가 혈액성상과 면역세포배양에 미치는 영향)

  • 송영선;김미정;권명자;송영옥;이은경;윤현주
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.6
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    • pp.1208-1214
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    • 1997
  • This study was designed to know the effect of kimchi on the hematological and immunological parameters in vivo and in vitro, respectively. To study the effects of kimchi on the hematological parameters, rats(S.D., male) were divided into 4 groups and fed diets containing of 3%, 5% and 10% kimchi or kimchi free diet(control) for 6 weeks. The results of CBC(complete blood cell) tests obtained from the bloods of rates were as follows ; In 10% kimchi group, the level of WBC(white blood cells), RBC(red blood cells), Hgb(hemoglobin), Hct(hematocrit) were increased significantly than those of control group(p<0.05). MCV(mean corpuscular volume), one of the red cell indices, was also increased significantly in the animals fed 10% kimchi(p<0.05). RDW(Red cell distritution width) and PCT(plateletcrit) was lowest in 10% kimchi group(p<0.05). To examine the effects of kimchi on immune cell growth in vitro, three types of mouse immune cells-spleen cells, bone marrow cells, thymus cells-were cultured with extracts of salted Chinese cabbage, fresh kimchi and fermented kimchi(for 1 week) for 12 or 20 days. Control was supplemented with PBS(phosphate buffer saline) excluding kimchi extract. The results of spleen cell, bone marrow cell, and thymus cell cultures showed similar tendency: control medium accelerated death of cells, extracts of salted Chinese cabbage reduced the rate of cell death, and extracts of fresh kimchi and fermented kimchi promoted cell growth. From these results, it could be suggested that kimchi possibly has an effect on the hematopoietic ability and increases immune cell development and growth in vivo.

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Studies on the Growth Rate of Silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae) Fed with Control and Silver Nanoparticles (AgNps) Treated MR2 Mulberry Leaves

  • Prabu, Ponraj Ganesh;Sabhanayakam, Selvi;Mathivanan, Veeranarayanan;Balasundaram, Dhananjayan
    • International Journal of Industrial Entomology and Biomaterials
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    • v.22 no.2
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    • pp.39-44
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    • 2011
  • To evaluate the growth rate of larval and pupal parameters of silkworm Bombyx mori fed with Silver Nanoparticles (AgNps) treated $MR_2$ mulberry leaves, the following works have been considered. The AgNp was synthesized by chemical reduction method, it was diluted by different concentrations such as 25%, 50%, 75% and 100% (without dilution). Fresh mulberry leaves (Morus alba L.) were sprayed by each concentration and were fed to silkworms, from $3^{rd}$, $4^{th}$ and $5^{th}$ instar, five feedings/day. Group $T_1$ larvae received $MR_2$ mulberry leaves sprayed with distilled water and served as control, group $T_2$, $T_3$, $T_4$ and $T_5$ larvae received 25%, 50%, 75% and 100% AgNps sprayed mulberry leaves, respectively. Silkworm larvae fed on M. alba ($MR_2$) leaves sprayed with 25% concentration of AgNps (group $T_2$) was significantly increased the larvae and cocoon length, width and weight as compared to those fed on control (group $T_1$) $MR_2$ mulberry leaves and other groups ($T_3$, $T_4$ and $T_5$). Hence, 25% AgNps dose was fixed as an effective dose. It has been observed from the present study that 25% AgNps treated (group $T_2$) leaves fed by silkworms have enhanced the larval and pupal growth and quantity of silk production than control.

Research on no coal pillar protection technology in a double lane with pre-set isolation wall

  • Liu, Hui;Li, Xuelong;Gao Xin;Long, Kun;Chen, Peng
    • Geomechanics and Engineering
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    • v.27 no.6
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    • pp.537-550
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    • 2021
  • There are various technical problems need to be solved in the construction process of pre-setting an isolation wall into a double lane in the outburst prone mine. This study presents a methodology that pre-setting an isolation wall into a double lane without a coal pillar. This requires the excavation of two small section roadways to dig a wide section roadway, followed by construction of the separation wall. During this process the connecting lane is reserved. In order to ensure the stability of the separation wall, the required bearing capacity of the isolation wall is 4.66 MN/m and the deformation of the isolation wall is approximately 25 cm. To reduce the difficulty of implementing support the roadway is driven by 5 m/d. After the construction of the separation wall, the left side coal wall is brushed 1.5 m to make the width of the gas roadway reach 2.5 m and the roadway support utilizes anchor rod, ladder beam, anchor cable beam and net configuration. During construction, the concrete pump and removable self-propelled hydraulic wall mold are used to pump and pour the concrete of the isolation wall. In the process of mining, the stress distribution of coal body and isolation wall is detected and measured on site. The results demonstrate that the deformation of the surrounding rock of roadway and separation of roof in the roadway is small. The stress of the bolt and anchor cable is within equipment tolerance validating their selection. The roadway is well supported and the intended goal is achieved. The methodology can be used for reference for similar mine gas control.

Application of rock mass index in the prediction of mine water inrush and grouting quantity

  • Zhao, Jinhai;Liu, Qi;Jiang, Changbao;Defeng, Wang
    • Geomechanics and Engineering
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    • v.30 no.6
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    • pp.503-515
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    • 2022
  • The permeability coefficient is an essential parameter for the study of seepage flow in fractured rock mass. This paper discusses the feasibility and application value of using readily available RQD (rock quality index) data to estimate mine water inflow and grouting quantity. Firstly, the influence of different fracture frequencies on permeability in a unit area was explored by combining numerical simulation and experiment, and the relationship between fracture frequencies and pressure and flow velocity at the monitoring point in fractured rock mass was obtained. Then, the stochastic function generation program was used to establish the flow analysis model in fractured rock mass to explore the relationship between flow velocity, pressure and analyze the universal law between fracture frequency and permeability. The concepts of fracture width and connectivity are introduced to modify the permeability calculation formula and grouting formula. Finally, based on the on-site grouting water control example, the rock mass quality index is used to estimate the mine water inflow and the grouting quantity. The results show that it is feasible to estimate the fracture frequency and then calculate the permeability coefficient by RQD. The relationship between fracture frequency and RQD is in accordance with exponential function, and the relationship between structure surface frequency and permeability is also in accordance with exponential function. The calculation results are in good agreement with the field monitoring results, which verifies the rationality of the calculation method. The relationship between the rock mass RQD index and the rock mass permeability established in this paper can be used to invert the mechanical parameters of the rock mass or to judge the permeability and safety of the rock mass by using the mechanical parameters of the rock mass, which is of great significance to the prediction of mine water inflow and the safety evaluation of water inrush disaster management.

Clinical evaluation of root coverage in Miller class III/RT2 labial gingival recession treated with interdisciplinary periodontal-orthodontic therapy: a randomized controlled clinical trial

  • Sakshi Malhotra;Shikha Tewari;Rekha Sharma;Rajinder Kumar Sharma;Nishi Tanwar;Ritika Arora
    • Journal of Periodontal and Implant Science
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    • v.54 no.4
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    • pp.265-279
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    • 2024
  • Purpose: The aim of current study was to evaluate percentage root coverage (RC%) in isolated Miller class III/RT2 labial gingival recession (GR) associated with malaligned mandibular anteriors, using interdisciplinary periodontal-orthodontic treatment as compared to mucogingival surgery alone. Methods: Thirty-six systemically healthy patients having isolated Miller class III/RT2 GR with respect to malaligned mandibular anteriors, were randomly divided into test group: mucogingival surgery using subepithelial connective tissue graft followed by orthodontic treatment and control group: mucogingival surgery alone. Primary clinical parameters included (RC%), recession depth, keratinized tissue width, mid-labial clinical attachment level, interdental clinical attachment level (iCAL), periodontal phenotype (PP), gingival thickness (GT), root coverage esthetics score (RES) and hypersensitivity. Total duration of follow up was 12 months. Results: Mean RC% was significantly more achieved in test group (66.67%±40.82%) in comparison to control group (39.93%±31.41%) at the end of study (P=0.049). Further, complete root coverage was attained in 5/8 cases of test group versus 1/2 cases of control group after 3/12 months respectively. RES and hypersensitivity, showed statistically significant improvement after complete follow up period in both the groups. An ideal RES score of 10 was achieved in 4/7 cases of test group while in 1/2 cases of control group after 3/12 months respectively. Correlation analysis revealed significant negative correlation between RC% and iCAL. Correlation of RC% with GT and PP was non-significant. Conclusions: Interdisciplinary periodontal-orthodontic approach may be more beneficial in terms of achieving improved RC%, esthetic and resolution of hypersensitivity in the management of Miller class III/RT2 GR in malaligned mandibular anteriors.

Development of a Rapeseed Reaping Equipment Attachable to a Conventional Combine (I) - Design and Construction of a Prototype - (보통형 콤바인 부착용 유채 예취장치 개발(I) - 시작기 설계 및 제작 -)

  • Lee, Choung-Keun;Choi, Yong;Jun, Hyun-Jong;Lee, Seung-Kyu;Ryu, Chan-Seok;Kim, Dong-Min
    • Journal of Biosystems Engineering
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    • v.33 no.6
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    • pp.371-378
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    • 2008
  • Bio-diesel applications seem to be extended due to bio-diesel policies and changes of agricultural environment. This study was conducted to develop a rapeseed reaping equipment attachable to the conventional combine. This paper was intended to report concept design, process and manufacturing of the prototype rapeseed reaping equipment. For concept design, physical properties of "SUNMANG", which is a typical rapeseed as bio-diesel materials, were considered. The designed prototype rapeseed reaping equipment consisted of wide-width plates, finger type knifes, side cutter knifes and drive equipments. The wide-width plate is 2.1 m wide, 0.7 m long, and 0.002 m thick. The finger type cutter knifes have 14.5 fingers, 30 knifes, and the specification was 7.6 cm of pitch, 8.3 cm of length and $21^{\circ}$ of cutting angle. The side cutter knifes consisted of a hydraulic pump, a hydraulic motor, a flow control and a relief valve, a hydraulic hose, a driving equipment and a reciprocating cutter knife. The 18 reciprocating cutter knifes were 137 cm long and knife pitch, knife length and cutting angle were 7.7 cm, 10.5 cm, and $18^{\circ}$. Prototype weight of the rapeseed reaping equipment was heavier by 272 kg when compared with the manual reaping equipments. Load distributions of left and right side showed 50% and 49%, and those of front and rear side showed 64% and 36%. Static turn-over angles in left and right of the prototype were $38.1^{\circ}$ and $38.7^{\circ}$, respectively. The designed prototype rapeseed reaping equipment was properly mounted at the front of a conventional combine.

Algorithm to determine injection position in olive flounder, Paralichthys olivaceus

  • Cha, Bong-Jin;Kwon, Mun-Gyeong;Kim, Hyun-Young;Park, Myoung-Ae
    • Journal of fish pathology
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    • v.26 no.2
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    • pp.59-64
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    • 2013
  • This study aimed to develop an algorithm to determine the optimal injection position in olive flounder, Paralichthys olivaceus when a vaccine is injected into the fish by using a vision-based automatic equipment measruing the total length (TL), width and weight of the fish. Over a 5-month period, 500 olive flounders were examined to analyze the relation of the fish size and to the shape and location of its abdomen, using radiography, a ruler plate and scale. There were significant correlations between the TL and the shape and location of the abdomen. The abdomen was located 0.232TL - 2.7221 mm ($R^2$=0.8787) from the end of the mouth of the fish. The height and width of the abdomen in the fish were 0.1292TL + 1.8768 mm ($R^2$=0.7935) and 0.183TL-5.9791 mm ($R^2$=0.8641), respectively. The injection point in the abdominal region avoiding organs was determined by calculating the center of gravity of the abdomen volume. This can be expressed as g (0.2759TL - 2.0965, 0.1295TL - 4.2325) on the basis of TL line coordinates. The injection point by the expressed coordinates had errors for the x and y axes as 12.15 mm and 8.28 mm, respectively. These were enough to use the algorithm to injection for the equipment. This automated method to determine the position of any part in the fish can also be used for other purposes, for example, intramuscular injection or auto-tagging of fish.