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Rapid Diagnosis of Resistance to Glufosinate-ammonium in Transgenic Sweet Potato (형질전환 고구마에 대한 Glufosinate-ammonium 저항성 간이진단법)

  • Kuk, Yong-In
    • Korean Journal of Weed Science
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    • v.30 no.4
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    • pp.380-389
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    • 2010
  • Transgenic herbicide-resistant sweet potato plants [Ipomoea batatas (L.) Lam.] produced through a biolistic transformation were used in this study. The objective of this research was to find out a rapid and reliable assay method for confirming glufosinate-ammonium resistance. The techniques tested include whole-plant bioassay, one leaf bioassay, and leaf disk bioassay. Parameters investigated in this study were leaf injury and ammonium accumulation at 1 and 5 days after treatment of glufosinate-ammonium. In the leaf disk bioassay, leaf injury of the transgenic line 7171 was 1.9-fold less affected by glufosinate-ammonium than the wild type. The leaf injury of 7171 in one leaf and whole-plant bioassays was 59- and 92-fold less affected by glufosinate-ammonium, respectively, compared with that of the wild type. Leaf disk, one leaf, and whole-plant bioassays showed that ammonium accumulation of the 7171 was 2 to 20-, 4 to 43-, and 6 to 115-fold less affected by 0.5-5 mM glufosinate-ammonium than that of the wild type. All three bioassays successfully distinguished the resistance from the transgenic lines, but one leaf bioassay is the simplest and quickest. Leaf injury and ammonium accumulation were the same in leaves 1, 3, 5, 7, and 10 of 3 mM glufosinate-ammonium treated plants or nontreated plants. The one leaf bioassay was chosen as the standard procedure for future confirmation of resistance in transgenic sweet potato because it is a rapid and reliable assay.

Development of Clubroot Resistant Doubled-Haploid Inbred Lines in Kimchi Cabbage (Chinese Cabbage) (Brassica rapa L.)

  • Park, Suhyoung;Jang, Hayoung;Park, Min Young
    • 한국균학회소식:학술대회논문집
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    • 2015.05a
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    • pp.37-37
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    • 2015
  • Kimchi cabbage (Chinese cabbage), radish and Cabbage are major Brassicaceae vegetables in Korea. Especially, we can easily develop whole plant from one microspore in Kimchi cabbage. To develop clubroot resistant doubled-haploid (DH) inbred lines, we pollinated a clubroot resistant turnip of 'IT 033820' with a Kimchi cabbage (Chinese cabbage) inbred of 'BP 079'. More than 85 DH inbred lines were developed from this combination. We screened about 400 materials including these DH inbred lines, commercial cultivars and breeding materials during 3 years using hydroponic system after inoculating single spore isolation race 4(SSI-04) inoculate. One inbred line derived from this combination selected as clubroot resistant and registered as 'Wonkyo20036ho'. We inoculated 26 DH inbred lines derived from 'Zoong-baek 2ho' using SSI-4, the percent of resistant plants varied from 0 to 83%. However the horticultural traits of highly resistant DH inbred line was poor. Thus we selected one DH line showing 77% resistant with yellow inner leaf and maid good head, was registered as 'Wonkyo20034ho'. Another DH inbred line derived from Korean variety of 'Wol-dong' showing 86% resistant was registered as 'Wonkyo20037ho'. Other DH inbred lines were derived from Chinese cultivar of 'Choon-hi-go-hang-wang' and 'Hwang-shim-zo48' showed 80 and 71% resistant, respectively, was also selected for registration. Even though DH inbred lines derived from turnip showed highly resistant to SSI-04 and provincial inoculate, they showed poor characteristics in horticultural traits. However, commercial seed companies showed interesting for adapting these DH inbred lines in commercial breeding.

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Outflow properties of DIGIT embedded sources

  • Kang, Seonmi;Lee, Jeong-Eun;Choi, Minho;Evans II, Neal J.;Dunham, Michael M.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.80.1-80.1
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    • 2015
  • We present a study of outflows on 24 embedded young stellar objects selected from the source list of the Dust, Ice, and Gas in Time (DIGIT) Herschel key program. To study the relation between the CO outflows observed in low-J transitions and the properties of protostars more consistently with a homogeneous data set, we mapped the CO outflows of the selected targets in the J = 1-0 and J = 2-1 lines with two Korean telescopes (SRAO and TRAO). We compare CO outflow force ($F_{CO}$) with the bolometric luminosity, ($L_{bol}$) bolometric temperature, and the FIR molecular line luminosities of CO, $H_2O$, OH, and [O I] detected by the Herschel-PACS observations. We find that $F_{CO}$ of J = 1-0 is greater than that of 2-1 by a factor of ~ 2. The well known correlation between $F_{CO\;2-1}$ and $L_{bol}$ is not very evident in our sample as a whole, but they show a rather strong correlation when IRAM 04191+1522 is excluded. IRAM 04191+1522 has relatively high $F_{CO\;2-1}$ in spite of its low $L_{bol}$. This object is a well-known VeLLO, which is believed in the quiescent phase of the episodic mass accretion in the embedded stage. $L_{bol}$ traces a current accretion, but $F_{CO\;2-1}$ traces accretion happened long ago. Therefore, the low-$L_{bol}$ with the high-$F_{CO\;2-1}$ can be explained by the episodic accretion. $F_{CO\;2-1}$ shows little correlation with individual FIR line luminosities of CO, $H_2O$, OH, while [O I] and total FIR line luminosity seem to have correlations with $F_{CO\;2-1}$. This result is interpreted as the accretion energy deposits on species differently depending on shock properties, but the total FIR line luminosity sums the total accretion energy dispersed to different species.

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A Study on the Shape of Hem-line of Semi-Flare Skirts according to a Cutting Angle - Based on the Comparison between Real Clothing and 3D Virtual Clothing - (재단 각도에 따른 세미 플레어 스커트의 외관 및 헴라인 드레이프 형상에 관한 연구 - 실제 착의와 i-Designer의 가상 착의 시스템을 중심으로 -)

  • Koo, Mi-Ran;Suh, Mi-A
    • The Research Journal of the Costume Culture
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    • v.17 no.3
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    • pp.499-511
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    • 2009
  • As the demand of the consumer for high-sense clothing, relation with materials is becoming important even in clothing construction. Especially, the cutting angle of materials is becoming an crucial element in the formation of silhouette, and drape of Hem line, of skirts. Accordingly, in this study, Hem line shapes between real clothing and 3D virtual clothing of "i-Designer" were analyzed by manufacturing semi-flare skirts of polyester 100% according to a cutting angle, and the results are as follows. As a result of comparison of silhouette between the real clothing and the virtual clothing, the real and the virtual have a similar feeling regardless of a cutting angle. In case of drape shapes, both the front and the lateral side were represented in almost similar shapes. The back side, however, the real and the virtual showed a great difference in case of grain direction. And in the whole silhouette, the real and the virtual were represented similarly. It could be known that with regard to Hem-line drape shapes between the real clothing and the virtual according to a cutting angle, the real clothing is represented in a location farther from the body than the virtual clothing and, the location or number of node was similarly showed in the real and the virtual.

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On-line Nonlinear Principal Component Analysis for Nonlinear Feature Extraction (비선형 특징 추출을 위한 온라인 비선형 주성분분석 기법)

  • 김병주;심주용;황창하;김일곤
    • Journal of KIISE:Software and Applications
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    • v.31 no.3
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    • pp.361-368
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    • 2004
  • The purpose of this study is to propose a new on-line nonlinear PCA(OL-NPCA) method for a nonlinear feature extraction from the incremental data. Kernel PCA(KPCA) is widely used for nonlinear feature extraction, however, it has been pointed out that KPCA has the following problems. First, applying KPCA to N patterns requires storing and finding the eigenvectors of a N${\times}$N kernel matrix, which is infeasible for a large number of data N. Second problem is that in order to update the eigenvectors with an another data, the whole eigenspace should be recomputed. OL-NPCA overcomes these problems by incremental eigenspace update method with a feature mapping function. According to the experimental results, which comes from applying OL-NPCA to a toy and a large data problem, OL-NPCA shows following advantages. First, OL-NPCA is more efficient in memory requirement than KPCA. Second advantage is that OL-NPCA is comparable in performance to KPCA. Furthermore, performance of OL-NPCA can be easily improved by re-learning the data.

A Study on Variation and Development of Railroad Station Zones on Incheon Metro Train Rail Line 1 (인천 1호선전철 역세권의 변화 및 개발에 관한 연구)

  • Park, Man-Hee;Ahn, Kyung-Su
    • Journal of the Korean Society of Hazard Mitigation
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    • v.9 no.3
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    • pp.27-39
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    • 2009
  • The 1st Incheon Rapid Transit Line opened in October, 1999. However, the current development plans for areas near stations do not involve plans for the both urban planning and transportation together. As the focus on only the aspect of installing a new transportation mode, the developments even make the problems such as reckless development and traffic congestion gets worse. It is required to analyse status of the current land uses and land use plans in the future and consider distribution of functions of the areas near stations and aims of development plans so as to harmonize the functions of each region in view of making balance in the whole city. The aims of developments of areas near stations has to focus on the study follows steps to find and suggest aims and strategies for the revitalization plan for areas near stations with considering the both transportation and land uses together. The plans for the areas near stations of the Incheon 1st Rapid Transit Line are analysed and then the features and rational strategies are derived. The impact is analysed as applying the development strategies derived from the step into the areas near the stations of Incheon 1st Rapid Transit line.

Quantitative Analysis of Developmental Process of Cranial Suture in Korean Infants

  • Sim, Sook-Young;Yoon, Soo-Han;Kim, Sun-Yong
    • Journal of Korean Neurosurgical Society
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    • v.51 no.1
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    • pp.31-36
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    • 2012
  • Objective : The purpose of this study was to elucidate the anatomical development of physiologic suture closure processes in infants using three dimensional reconstructed computed tomography (CT). Methods : A consecutive series of 243 infants under 12 months of age who underwent three dimensional CT were included in this study. Four major cranial sutures (sagittal, coronal, lambdoidal and metopic suture) were classified into four suture closure grades (grade 0=no closure along the whole length, grade 1=partial or intermittent closure, grade 2=complete closure with visible suture line, grade 3=complete fusion (ossification) without visible suture line), and measured for its closure degree (suture closure rates; defined as percentage of the length of closed suture line divided by the total length of suture line). Results : Suture closure grade under 12 months of age comprised of grade 0 (n=195, 80.2%), grade 1 (n=24, 9.9%) and grade 2 (n=24, 9.9%) in sagittal sutures, whereas in metopic sutures they were grade 0 (n=61, 25.1%), grade 1 (n=167, 68.7%), grade 2 (n=6, 24%) and grade 3 (n=9, 3.7%). Mean suture closure rates under 12 months of age was 58.8% in metopic sutures, followed by coronal (right : 43.8%, left : 41.1%), lambdoidal (right : 27.2%, left : 25.6%) and sagittal sutures (15.6%), respectively. Conclusion : These quantitative descriptions of cranial suture closure may help understand the process involved in the cranial development of Korean infants.

Characteristics of Somatotype Classified by the Drop Value of Middle-Aged Women (중년 여성의 드롭치에 따른 체형 특성 연구)

  • Yoon, Ji-Won;Suh, Mi-A
    • The Research Journal of the Costume Culture
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    • v.17 no.6
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    • pp.939-946
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    • 2009
  • This study is to classify the shape of the body by means of the drop value of the middle-age women. Drop value is based on the circumstances of the body deciding the dimentions of the clothes. Therefore, the study based on the drop value seems to decided the measurement of the clothes in making mass production of ready made clothes. In order to put measure items on this study of the value on 'The fifth survey of body measure of Korean', select 785 people in middle-aged women aged from 40 to 59. And considering the characteristic of upper-drop and lower-drop, and swelling belly by accumulated fat, selected five items(Bust Circumstance - Waist Circumstance, Hip Circumstance - Waist Circumstance, Hip Circumstance - Bust Circumstance, Waist Circumstance(Omphalion) - Waist Circumstance, Hip Circumstance - Waist Circumstance(Omphalion)). The result of the study is summarized as follows. Type 1, M type of the body showed the distribution of 32.5% and belonged to low body development style. The line of human body is the nearest of the four bodies in middle-age woman out of whole shapes of 4 types and the line of human body is close to middle-age woman. Type 2, X type of the body showed the distribution of 40.8% and the line of human body remarkably showed. Type 3, H type of the body showed the distribution of 12.4% and the line of human body appears least. This group was larger than any other group in having more fat in the belly above the circumstance of the hip than other groups. Type 4, Y type of the body showed the distribution of 14.4%. This type was upper half of body development style, waist was slender, and there was seldom the bend around the circumstance of belly and hip.

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Compact and Broadband 90° Coupler Using a Metamaterial (메타 물질을 이용한 초소형, 광대역 90° 커플러)

  • Kim, Hong-Joon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.7
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    • pp.844-847
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    • 2012
  • By using LHTL(Left-Handed Transmission Line) which is a form of a metamaterial and conventional RHTL (Right-Handed Transmission Line), we designed, fabricated and tested a broadband $90^{\circ}$ coupler which is a basic circuit for I-Q vector signal generation. Synthetic LHTL and RHTL were implemented with capacitors and inductors only, that the size is minimized. Also, by implementing a Wilkinson power divider which is required for the suggested circuit using a synthetic RHTL, the size of whole circuit is only $11mm{\times}12mm$. For the frequency range 0.8~1.25 GHz, the phase difference at the outputs maintained $90^{\circ}{\pm}5^{\circ}$ and thus, a broadband $90^{\circ}$ coupler could be made in a compact form. for the same frequency range, the insertion loss is less than 1.6 dB and return loss is more than 10.1 dB. To the best of our knowledge, this is the smallest and broadband $90^{\circ}$ coupler for the frequency range and if the circuit is made with MMIC(Monolithic Microwave Integrated Circuit) technology, the size will be reduced much further.

Uncertainty Observer using the Radial Basis Function Networks for Induction Motor Control

  • Huh, Sung-Hoe;Lee, Kyo-Beum;Ick Choy;Park, Gwi-Tae;Yoo, Ji-Yoon
    • Journal of Power Electronics
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    • v.4 no.1
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    • pp.1-11
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    • 2004
  • A stable adaptive sensorless speed controller for three-level inverter fed induction motor direct torque control (DTC) system using the radial-basis function network (RBFN) is presented in this paper. Torque ripple in the DTC system for high power induction motor could be drastically reduced with the foregoing researches of switching voltage selection and torque ripple reduction algorithms. However, speed control performance is still influenced by the inherent uncertainty of the system such as parametric uncertainty, external load disturbances and unmodeled dynamics, and its exact mathematical model is much difficult to be obtained due to their strong nonlinearity. In this paper, the inherent uncertainty is approximated on-line by the RBFN, and an additional robust control term is introduced to compensate for the reconstruction error of the RBFN instead of the rich number of rules and additional updated parameters. Control law for stabilizing the system and adaptive laws for updating both of weights in the RBFN and a bounding constant are established so that the whole closed-loop system is stable in the sense of Lyapunov, and the stability proof of the whole control system is presented. Computer simulations as well as experimental results are presented to show the validity and effectiveness of the proposed system.