• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.166-176
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• 2020

Objective: An experiment was conducted to determine the effects of L-arginine (L-Arg) and N-carbamoylglutamic acid (NCG) on the growth, metabolism, immunity and community of cecal bacterial flora of weanling and young rabbits. Methods: Eighteen normal-grade male weanling Japanese White rabbits (JWR) were selected and randomly divided into 6 groups with or without L-Arg and NCG supplementation. The whole feeding process was divided into weanling stage (day 37 to 65) and young stage (day 66 to 85). The effects of L-Arg and NCG on the growth, metabolism, immunity and development of the ileum and jejunum were compared via nutrient metabolism experiments and histological assessment. The different communities of cecal bacterial flora affected by L-Arg and NCG were assessed using high-throughput sequencing technology and bioinformatics analysis. Results: The addition of L-Arg and NCG enhanced the growth of weanling and young rabbit by increasing the nitrogen metabolism, protein efficiency ratio, and biological value, as well as feed intake and daily weight gain. Both L-Arg and NCG increased the concentration of immunoglobulin A (IgA), IgM, and IgG. NCG was superior to L-Arg in promoting intestinal villus development by increasing villus height, villus height/crypt depth index, and reducing the crypt depth. The effects of L-Arg and NCG on the cecal bacterial flora were mainly concentrated in different genera, including Parabacteroides, Roseburia, dgA-11_gut_group, Alistipes, Bacteroides, and Ruminococcaceae_UCG-005. These bacteria function mainly in amino acid transport and metabolism, energy production and conversion, lipid transport and metabolism, recombination and repair, cell cycle control, cell division, and cell motility. Conclusion: L-Arg and NCG can promote the growth and immunity of weanling and young JWR, as well as effecting the jejunum and ileum villi. L-Arg and NCG have different effects in the promotion of nutrient utilization, relieving inflammation and enhancing adaptability through regulating microbial community.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.515-524
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• 2010

The amylose content of starch is a major factor in the texture of cooked cereal grains. Therefore, down-regulation of amylose synthesis is one of the alternative method to improve eating quality of rice. We developed transgenic rice plants designed to suppress granule-bound starch synthase I(GBSSI) gene using RNA interference(RNAi) technology. Transgenic plants with RNAi vector containing the 3'-UTR region of GBSSI showed a lower amylose content in rice endosperm than that of wild-type. The range of amylose content was 5.9~9.0% in the transgenic plants, whereas that of wild-type was 17.7~18.0%. Transgenic rices showed the decrease of short chain and the increase of long chain by analyzing chain length distribution of amylopectin in the endosperm. In the SEM micrographs, we found that compound starch granules in whole grains of the wild-type rice were readily split during fracturing, while the starch granules in RNAi-transgenic lines showed small voluminous, non-angular rounded bodies.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.484-489
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• 2008

A new whole crop silage barley cultivar, "Youngyang", was developed from the cross between "Bunong and Milyang55 at the Yeongnam Agricultural Research Institute (YARI) in 2002. An elite line, YB3882-3B-17-1-3-1, was selected in 1999 and designated as "Milyang111". It showed good agronomic performance in the regional adaptation yield trials (RYT) from 2000 to 2002 and was released by the name of "Youngyang" with higher yield and better animal feed quality than that of Olbori. The average heading and maturing dates of Youngyang were May 2 and June 6, which were late by 2 & 1 days than those of Olbori, respectively, in Suwon. Youngyang had longer culm length, 83cm but it had lesser spikes per $m^2$ 637 than that of Olbori, respectively, in RYT from 2000 to 2002. The rate of leaf sheath/whole length (culm+leaf) and the rate of the grains/whole crop weight was similar to Olbori. It showed resistance to BaYMV at the regions of Naju, Jinju, Milyang andt Iksan. however, the response of Youngyang to other environmental stresses (tolerance of cold and wet) was similar to Olbori. The dried whole crop yield potential of Youngyang in the RYT was about 11.67 MT/ha in paddy fields which was about 18% higher than that of Olbori. The grain yield potential was 6.32 MT/ha in paddy fields which was about 5% higher than that of Olbori. Youngyang had lower rate of shattering, higher content of protein and NDF, lower ADF and TDN, and better quality of silage than those of Olbori.

• Journal of Practical Agriculture & Fisheries Research
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• v.11 no.1
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• pp.103-111
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• 2009

This study was carried out to determine effects of long-term supply of Cordyceps militaris mycelia on cordycepin content in milk in dairy cows. Ten Holstein cows in the early stages of lactation were divided into two groups. Control group received no supplement whereas treatment group received 6% of C. militaris mycelia of their dry matter intake for 6 months. Feed intake, milk urea nitrogen and somatic cell counts were not affected by long-term supply of C. militaris mycelia for the whole period. In addition, milk yield and milk composition were not affected by long-term supply of C. militaris mycelia at any time of the periods with the exception of milk protein content and yield. The average of milk protein content and yield from the whole period was higher for C. militaris mycelia supplement group than for the control group. As expected, cordycepin in whole blood and milk was not detected in the control group. The range of cordycepin content in the treatment was 0.31~0.38µ/ml and 0.18~0.26(µ/ml for whole blood and milk, respectively. Individual variation was found to be very high and, furthermore cordycepin was undetected in some milk samples. Thus, no clear pattern could be seen in cordycepin content in milk throughout the whole period. Overall, the results of the present study suggest that the transfer efficiency of cordycepin to milk by supplementing C. militaris mycelia in dairy cows was unpredictable and low.

• Journal of Practical Agriculture & Fisheries Research
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• v.3 no.1
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• pp.116-131
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• 2001

Experiments were conducted to determine effects of whole and processed corns on in situ disappearance rates of nutrients in the rumen and in vitro degradability of dry matter by rumen microorganisms. Whole corn(WC) was processed into four different types; ground corn(GC), cracked corn(CC), flaked corn(FC), and soaked corn(SC). In the in situ experiment, the rate of ruminal DM disappearance after 48 hour incubation was highest in GC(76.1%) and lowest in WC and SC(12% ; P<0.01). The rate of ruminal CP disappearance after 48 hour incubation was highest in GC and CC(48 and 38%, respectively; P<0.01). The rate of ruminal OM disappearance after 48 hour incubation was highest in GC(76.14%) and lowest in WC and SC(11.82 and 12.26%, respectively; P<0.01). In the in vitro experiment, the two-stage incubation technique was used to measure digestibilities of whole and processed corns. The digestibility of DM was higher in GC, CC and FC(86.95, 85.84 and 82.29%, respectively) than in WC(15.36%; P<0.01).

• Korean Journal of Organic Agriculture
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• v.21 no.3
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• pp.451-465
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• 2013

This study was conducted to estimate Hanwoo carrying capacity when whole crop barley, rye, wheat and tritcale as winter forage crops was grown on different applying sources (chemical fertilizer, cattle or organic fertilizer) and mixed sowing combination with hairy vetch or forage pea during the period of 2011~2012. The experimental plots within whole crop barley or rye were consisted of 7 treatments, which were non-fertilizer, chemical fertilizer (P+K), chemical fertilizer (N+P+K), organic fertilizer, cattle slurry, cattle slurry with hairy vetch, and cattle slurry with forage pea. Each plot was triplicates and experimental treatments were allocated in the randomized complete block design. For whole crop barley, annual mean dry matter (DM) and total digestible nutrients (TDN) yields were the highest in N+P+K plots, but there were no significant differences among organic fertilizer, cattle slurry and mixed sowing with legumes. The TDN were the highest in mixed sowing plots of forage pea plus cattle slurry application. As 450 kg Hanwoo heifers were fed diets included 70% whole crop barley, organic fertilizer, cattle slurry application and mixed sowing plots of forage pea is capable of raising average 2.8 to 3.1 heads/ha a year. For whole crop rye, annual mean DM were the highest in N+P+K plots, but there were no significant differences among cattle slurry. Organic fertilizer application significantly increased TDN and relative feed value (RFV) in comparison with treatments of N+P+K fertilization as chemical fertilizers. In case of 450 kg Hanwoo heifers fed diets included 70% forage rye, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 2.8~ 3.2 heads/ha a year. For whole crop wheat, annual DM, crude protein, and TDN yields of application groups and mixed sowing treatment with legumes showed 6.90~7.44, 0.53~0.60 and 4.35~5.04 ton/ha, respectively. In case of 450 kg Hanwoo heifers fed diets included 70% forage rye, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 3.1~3.7 heads/ha a year. For Triticale, TDN yield was significantly (P<0.05) higher N+P+K plots, organic ferilizer, cattle slurry, cattle slurry with legumes than for no fertilizer and N+P+K plots. The Crude protein (CP) contents were the highest in mixed sowing plots of forage pea plus cattle slurry application. In case of 450 kg Hanwoo heifers fed diets included 70% forage triticale, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 3.4~3.7 heads/ha a year. It can be concluded that, on the basis of DM yield, not only mixed sowing with legumes by applying cattle slurry rather than single sowing of whole crop barley or whole crop rye enhanced production yield and feed values, but also it could be a substitute for imported grains as dietary protein sources in the case of feeding Hanwoo.

• Nutrition Research and Practice
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• v.5 no.4
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• pp.322-328
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• 2011

The aim of this study was to use the Healthy Eating Index-2005 (HEI-2005) to assess diet quality and determine the relationship between the HEI-2005 and the energy and nutrient intakes of adolescents. A cross-sectional study was conducted on 1,104 healthy adolescents, mean age of $15.8{\pm}1.24$ years. Dietary intake was measured with the 24-hour dietary recall method, and dietary quality was assessed by means of the HEI-2005. Diet quality scores ranged from 23.7 to 77.5. The mean score was found to be $51.5{\pm}9.07$ according to the HEI-2005. There were no differences according to gender, 42.8% had a poor diet and 57.2% had a diet that needs improvement. No subjects had a "good diet". Lower mean subgroup scores were found for whole grains, total vegetables, total fruits, dairy products, and meat and beans. Fruits and vegetables scores were significantly high in girls, whereas sodium, oil, and meat and beans scores were significantly high in boys. Total HEI-2005 scores were increasingly associated with parental education level when age and gender were adjusted. There was a negative correlation between HEI-2005 scores and age, total energy intake, and fat intake. Positive correlation was only observed in the HEI-2005 scores for protein and dietary fibre intakes. Consequently, the overall diet quality and nutritional habits of Turkish adolescents need modification and improvement. In the family, measures should be initiated by the government, including advertisements and campaigns.

• Korean Journal of Agricultural Science
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• v.26 no.1
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• pp.12-20
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• 1999

The purpose of this study was to research the change of the forest in Myungseo-lee Sanchuk-myun, Chungju where most of the area managed the Swidden Agriculture(Shifting cultivation). The mountainous district should be used and developed the multi-purposing source among the agricultural field, grassland, forest, a recreational area, and an industrial site, and should be presupposed the establishment of synthetic and efficient change system. This study began to pay attention to observe the peculiarity of Swidden Agricultural area in terms of forest aspects and to broaden the outlook of Swidden Agriculture. In the status of Myungseo-lee, prior to the Project to Rehabilitate the Illegal Swidden, from 1973 to 1976, the private forest gets 110.9ha (23ha for Seodae-village, 26ha for Dooduk-village, 6ha for Myungdol-village, 41ha for Chungam-village, 14.9ha for Pangdae-village). In Myungseo-lee. millet and corn were harvested, and they performed the rotation of planting and temporary resting by changing the field. The cultivated acreage per a house of total 5 villages in Myungseo-lee was just 0.4ha in average. The 70ha of the Swidden in Dooduk-village. Myungseo-lee, was sold to the Miwon corp. and the 342ha of that in Myungseo-lee was sold to Seohae-devlp.(SK forest). Those areas afforested. As the regulation plan for the Swidden began, the people in the site got paid for 300,000won as a migration fee. The special feature of the Swidden in Myungseo-lee was that the whole migration village was already established before the regulation plan for the Swidden began, and the yield was harvested by more than other villages the use of chemical fertilizer. And, in the Swidden agriculture, it showed the well-cultivated aspect, and the major harvest was various grains. The regulation Swidden in Myungseo-lee started in 1974.

• Journal of Nutrition and Health
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• v.7 no.4
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• pp.6-11
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• 1974

1. The status of nutrition hasn't been found a great difference between the model village and the compared one, so there should be a great need of the improvement of the traditional food life which is chiefty consisted of some cereal grains and vegetables, in a Larger sense, the mixing diet would diminish the burden of stomach and help us to get high nutrition with a bit of quantify. 2. The health of the oral cavity has a close relation with that of whole body. In order to keep a good dental health, there fore, we should study and try to get more calcium in another food group except in the vegetarian diet. The pregnant women must especially have much recognition to the necessity for getting a lot of calcium. 3. It is desirable to clean our teeth three times every day after having meals for every three minutes. The habit which is called 'three three three campaign' should clearly be kept for all of us.

• Nutrition Research and Practice
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• v.5 no.3
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• pp.192-197
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• 2011

The dietary intake of whole grains is known to reduce the incidence of chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer. To investigate whether there are anti-adipogenic activities in various Korean cereals, we assessed water extracts of nine cereals. The results showed that treatment of 3T3-L1 adipocytes with Sorghum bicolor L. Moench, Setaria italica Beauvois, or Panicum miliaceum L. extract significantly inhibited adipocyte differentiation, as determined by measuring oil red-O staining, triglyceride accumulation, and glycerol 3-phosphate dehydrogenase activity. Among the nine cereals, P. miliaceum L. showed the highest anti-adipogenic activity. The effects of P. miliaceum L. on mRNA expression of peroxisome proliferator-activated receptor-${\gamma}$, sterol regulatory element-binding protein 1, and the CCAAT/enhancer binding protein-${\alpha}$ were evaluated revealing that the extract significantly decreased the expression of these genes in a dose-dependent manner. Moreover, P. miliaceum L. extract changed the ratio of monounsaturated fatty acids to saturated fatty acids in adipocytes, which is related to biological activity and cell characteristics. These results suggest that some cereals efficiently suppress adipogenesis in 3T3-L1 adipocytes. In particular, the effect of P. miliaceum L. on adipocyte differentiation is associated with the downregulation of adipogenic genes and fatty acid accumulation in adipocytes.