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Isolation of an Anticariogenic Compound from Magnoliae Bark (후박피(Magnoliae bark)로부터 항충치활성을 갖는 물질의 분리)

  • Lee, Youn-Soo;Park, Hun-Joo;You, Jae-Sun;Park, Hyung-Hwan;Kwon, Ik-Boo;Lee, Hyeon-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.1
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    • pp.230-236
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    • 1998
  • We have screened total 32 herbal drugs to find the highest activity against human cariogenic enzyme, glucosyltransferase (GTase) from the extracts of Magnoliae bark. The extracts were separated into three phases, i. e. water, n-butanol and ethylacetate according to their solvent polarity. Among them, ethylacetate fraction had approximately more than 70% of total activities, and the active principle was further isolated by prep. HPLC following silicagel column chromatography to yield single compound as white powder. The chemical structure of the compound was finally elucidated to be 4,4'-dihydroxy-3,3'-dimethoxylignan from the spectral data of FAB-MS. $^1H-\;and\;^{13}C-NMR$ spectrometries. The compound was also shown to have relatively strong antibacterial activity against ten types of cariogenic oral bacteria and one kind of Actinomyces sp.

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Effect of Simvastatin collagen graft on wound healing of defective bone (Simvastatin이 골결손부 회복에 미치는 영향)

  • Kang, Jung-Ho;Kim, Gyu-Tae;Choi, Yong-Suk;Lee, Hyeon-Woo;Hwang, Eui-Hwan
    • Imaging Science in Dentistry
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    • v.38 no.3
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    • pp.133-146
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    • 2008
  • Purpose : To observe and evaluate the effects of Simvastatin-induced osteogenesis on the wound healing of defective bone. Materials and Methods : 64 defective bones were created in the parietal bone of 32 New Zealand White rabbits. The defects were grafted with collagen matrix carriers mixed with Simvastatin solution in the experimental group of 16 rabbits and with collagen matrix carriers mixed with water in the controlled group. The rabbits were terminated at an interval of 3, 5, 7, and 9 days, 2, 4, 6, and 8 weeks after the formation of defective bone. The wound healing was evaluated by soft X-ray radiography. The tissues within defective bones were evaluated through the analysis of flow cytometry for the manifestation of Runx2 and Osteocalcin, and observed histopathologically by using H-E stain and Masson's trichrome stain. Results : 1. In the experimental group, flow cytometry revealed more manifestation of Runx2 at 5, 7, and 9 days and Osteocalcin at 2 weeks than in the controlled groups, but there was few difference in comparison with the controlled group. 2. In the experimental group, flow cytometry revealed considerably more cells and erythrocytes at 5, 7, and 9 days in comparison with the controlled group. 3. In the experimental group, soft x-ray radiography revealed the extended formation of trabeculation at 2, 4, 6, and 8 weeks. 4. Histopathological features of the experimental group showed more fibroblasts and newly formed vessels at 5 and 7 days, and the formation of osteoid tissues at 9 days, and the newly formed trabeculations at 4 and 6 weeks. Conclusion : As the induced osteogenesis by Simvastatin, there was few contrast of the manifestation between Runx2 and Osteocalcin based on the differentiation of osteoblasts. But it was considered that the more formation of cells and erythrocytes depending on newly formed vessels in the experimental group obviously had an effect on the bone regeneration.

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First Report of Curvularia Leaf Blight Caused by Curvularia trifolii on Creeping Bentgrass in Korea (Curvularia trifolii에 의한 크리핑벤트그래스 잎마름병 발생)

  • Sung, Chang-Hyun;Koo, Jun-Hak;Kim, Jung-Ho;Yoon, Jung-Ho;Lee, Jung-Han;Shim, Kyu-Yul;Kwak, Youn-Sig;Chang, Seog-Won
    • Weed & Turfgrass Science
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    • v.5 no.2
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    • pp.101-104
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    • 2016
  • Curvularia leaf blight of creeping bentgrass (Agrostis stolonifera) putting green by caused Curvularia trifolii was observed in Hapcheon, Korea. In July to September 2014, curvularia leaf blight developed on leaf blades of creeping bentgrass as small water-soaked lesions that subsequently turned into dark-colored, necrotic spots. The spots were expanded and became gray, grayish-brown, or light brown, circular to oblong lesions with purple to dark brown borders that often were surrounded by a yellow halo. The necrotic lesions coalesced, became irregular in shape and caused tip or complete blighting of the leaves. Blighted leaf blades appeared grayish-white to tan. The fungus was identified by morphological characters and 16S rDNA sequencing as C. trifolii. Conidia of the pathogen were short, with predominantly 3-septa, straight or often curved, with end cells frequently paler than intermediate cells. Size of the 3-septate conidia in culture are $26{\sim}28{\times}11{\sim}12{\mu}m$. Pathogenicity of the fungus was proved by artificial inoculation on the host. This is the first report of C. trifolii causing leaf blight on creeping bentgrass in Korea.

Spatio-temporal Distribution Pattern of New Biotypes of Weedy Rice (Oryza sativa L.) in Selangor North-West Project, Malaysia

  • Baki, B.B.;M.M., Shakirin
    • Korean Journal of Weed Science
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    • v.30 no.2
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    • pp.68-83
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    • 2010
  • Weedy rice (Oryza sativa L.) occurred sympatrically with other weeds and the rice crop in Malaysian rice granaries. We conducted field surveys in 2006-2008 seasons in 7 farm blocks of Selangor's North West Project, Malaysia to enlist the new biotypes of weedy rice (NBWR) and assess their spatio-temporal pattern of distribution based on quantitative and dispersion indices. No less than 16 accessions of NBWR were identified based on their special traits, viz. panicle type, pericarp colour, presence or absence of awn, seed type and degree of grain shattering. The NBWR accessions exhibited a combination of morphological traits from open panicle, grain with awns, red pericarp, short grain type, and degrees of grain shattering. Others mimic commercial rices with close panicle, awnless grains, white pericarp, long or short grain-type. Invariably, the NBWRs mimic and stand as tall as cultivated rice namely MR219, MR220, or MR235 and these NBWR accessions stand among equals morphologically vis-a-vis the commercial rice varieties. Most accessions displayed varying degrees of grain shattering in excess of 50%, except Acc9 and Acc12. The seasonal dynamics of on the prevalence of dominant NBWR accessions were also displaying significant differences among farm blocks. While Bagan Terap farm block, for example, did not record any measurable changes in the dominant NBWR accessions over seasons, the Sungai Leman farm block recorded measurable season-mediated changes in the dominant NBWR accessions. Sungai Leman started with NBWR Acc3, Acc4, Acc5, Acc7, Acc8, and Acc12 in season 1 of 2006/2007, but no measurable records of Acc3 and Acc5 were shown in season 2 of 2007. In season 3 of 2007/2008, only Acc8 and Acc12 prevailed in the farm block. In Sawah Sempadan farm block, season 3 of 2007/2008 showed much reduced prevalence of NBWRs leaving only Acc8 and Acc12. Most accessions registered clump or under-dispersed spatial distribution pattern based on quantitative indices: variance-to-mean ratio (VMR) and Lloyd's patchiness values. The dynamics on the extent of infestation and prevalence of dominant NBWR accessions registered both season- and farm-block mediated differences. Most accessions showed VMR >1 thus indicative of having a clump or clustered spatial distribution, as exemplified by Acc3, Acc4, Acc7, Acc8 and Acc12 in all farm blocks throughout three seasons. Some accessions have either random or uniform distribution in a few farm blocks. The Acc8 has the highest population counts based on important value index, followed by Acc12, and both were the most dominant accession while Sawah Sempadan was the worst farm block infested by NBWR compared to other farm blocks. These results were discussed in relation with the current agronomic and weed management practices, water availability and extension services in the granary.

Effect of Silicon on the Corrosion Characteristics of Zirconium (Zr의 부식특성에 미치는 Si의 영향)

  • Jeon, Chi-Jung;Kim, Hee-Suk;Kim, Yong-Deok;Hong, Hyun-Seon;Kim, Seon-Jin;Lee, Kyung-Sub
    • Korean Journal of Materials Research
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    • v.8 no.6
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    • pp.513-519
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    • 1998
  • Zr-Si binary alloys containing 0.01 to O.lwt.%Si were prepared to investigate the effect of Si on the corrosion behavior of Zr. Corrosion test was performed in pure water at 36$0^{\circ}C$ under a pressure of 2660psi for 100days. The alloys containing 0.01 wt. % and 0.05wt. %Si had the black and uniform oxide film and didn't show the transition of corrosion rate. However. the alloys containing O.lwt.%Si had white oxide film and showed the trasition of corrosion rate at 70 days corrosion test. The weight gain increased with the increasing Si content from 0.01 to 0.1 wt.%. The variation of Si contents had no effect on changing the oxide structure but had significant effect on the electrical resistivity of oxide. The electrical resistivity decreased with increasing Si content. The fraction of precipitates in the Zr-Si binary alloys. identified as tetragonal $Zr_{3}$Si increased with increasing Si content. The increase of the volume fraction of precipitates is thought to be responsible for the increase of weight gain due to short circuit effect of precipitate.

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Software Development for the Visualization of the Orientation of Brain Fiber Tracts in Diffusion Tensor Imaging Using a 24 bit Color Coding

  • Jung-Su Oh;In Chan Song;Ik-Hwan Cho;Jong-Hyo Kim;Kee Hyun Chang;Kwang-Suk Park
    • Journal of Biomedical Engineering Research
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    • v.25 no.1
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    • pp.43-47
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    • 2004
  • Interests in human brain functionality and its connectivity have much frown up. DTI (Diffusion tensor imaging) has been known as a non-invasive MR) technique capable of providing information on water diffusion in tissues and the organization of white matter tract. Thus. It can provide us the information on the direction of brain fiber tract and the connectivity among many important cortical regions which can not be examined by other anatomical or functional MRI techniques. In this study. was used the 24 bit color coding scheme on the IDL platform in the windows environment to visualize the orientation of major fiber tracts of brain such as main association, projection, commissural fibers and corticospinal tracts. We additionally implemented a color coding scheme for each directional component and FA (fractional anisotropy), and used various color tables for them to be visualized more definitely. Consequently we implemented a fancy and basic technique to visualize the directional information of fiber tracts efficiently and we confirmed the feasibility of the 24 bit color coding scheme in DTI by visualizing main fiber tracts.

Induced Spawning Behavior and Morphological Development of the Eggs and Larvae of the Variable Sabretooth Blenny, Petroscirtes variabilis (Pisces: Blenniidae) (개베도라치(Petroscirtes variabilis)의 산란행동 유도 및 난 발생, 자어의 형태발달)

  • Park, Jae Min;Han, Kyeong Ho;Kim, Na Ri;Cho, Jae Kwon
    • Korean Journal of Ichthyology
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    • v.26 no.4
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    • pp.267-273
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    • 2014
  • This research has carried out to make a data base of taxonomic research. That Data base explains about spawning behavior, egg development, and morphologic development of variable sabretooth blenny. Fertilized egg was demersal egg which is white and opaque. The number of oil glouble were 10~11, and the size of egg was 0.90~1.43 mm (average rate $1.11{\pm}0.23mm$, n=10). Breeding water temperature was $25.5{\sim}28.5^{\circ}C$ (average rate $27.0^{\circ}C$), and salinity was 32.5~33.5‰ (average rate 33.0‰). After 24 hours from 2 cells, the process of egg development was reached to Blastula stage. Moreover, after 330 hours from 2 cells, nostrils and eyes were formed. Egg membrane was pierced by the head, and the hatch began. After the hatch, postlarvae had 2.59~3.02 mm (average rate $2.81{\pm}0.25mm$, n=5) of whole length, and the mouth and anus were opened. Yolk sac and oil glouble were absorbed. After three days from hatch, prelarvae were 3.02~3.07mm(average rate $3.04{\pm}0.04mm$) of whole length, and caudal fin was grown with round shape. After 13 days from hatch, prelarvae had 3.04~3.20 mm (average rate $3.12{\pm}0.11mm$) of whole length, and they could eat food with upper jaw and bottom jaw.

Preparation and Characteristics of Calcium Lactate from Black Snail

  • Lee, Ye-Kyung;Kim, Soon-Dong
    • Preventive Nutrition and Food Science
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    • v.8 no.2
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    • pp.166-172
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    • 2003
  • Calcium lactate (CL) prepared from powdered black snail (PBS) or its ashed powder (ABS), was investigated for ideal manufacturing conditions to optimize color, solubility and sensory quality. Based on the amount of PBS and 100 mL lactic acid (LA), the yields of PBS-CL were 300% and 15 g in 10% LA and 260% and 20 g in 20% LA. Yields of ABS-CL based on the amount ABS and 100 mL LA were 400% and 60 g in 10% LA and 329% and 66 g in 20% LA. Both of the yields were decreased with an increase of the LA concentration on the basis of PBS and ABS amounts, but proportionally increased with the increment in the LA concentration on the basis of LA volume. Optimal preparation times of the dehydrated PBS-CL and ABS-CL were, respectively, 4 hr and 5 hr at 10$0^{\circ}C$, 3 hr and 4 hr at 12$0^{\circ}C$, and 1 hr and 2 hr at 15$0^{\circ}C$, which showed shorter time in preparing the dehydrated ABS-CL. PBS-CL and ABS-CL were confirmed to be Ca($CH_3$CHOH$CO_2$)$_2$ by the analysis results of IR and $^1$H-NMR. Calcium contents of the anhydrous PBS-CL and ABS-CL were individually 15.4% (w/w) and 17.3% (w/w) representing 84.2% and 94.5% or each theoretical value. Colors or PBS-CL and ABS-CL were light yellow and light-greenish white each. Solubilities of PBS- CL and ABS-CL in distilled water at pH 3~8 were 5.43 and 6.11 g/100 mL, respectively, which demonstrated higher mean solubilities rather than the 4.74 g/100 mL of standard CL. Solubilities of PBS-CL (3.14~5.03 g/100 mL) and ABS-CL (4.69~6.05 g/100 mL) against soup soy sauce, 3% brine, Soju (Korean distilled liquor), thick soy sauce, grape juice and orange juice were higher than those of standard CL (2.94~5.84 g/100 mL). ABS-CL was believed to have a wide use range due to its low sourness while different applications of PBS-CL in food are expected due to its mild astringent taste and strong savory taste despite its strong bitter taste as estimated by sensory evaluation.

Functional Properties of Mungbean Protein Isolates (분리녹두단백의 식품기능적 특성에 관한 연구)

  • Kye, In-Sug;Jun, Yeong-Soo;Cheigh, Hong-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.3
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    • pp.300-306
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    • 1989
  • This study was investigated to determine the functional properties of mugbean protein isolates(MPI) from sunhwa-nogdu(SH) and conventional mungbean varieties(C). MPI were prepared from defatted mungbean flour by extraction with 0.1N NaOH, precipitation at pH 4.5, washing of dispersed precipitate with buffer solution and distilled water, and subsequent freeze-drying. Crude protein content of MPI was in the range of $88.7{\sim}91.3%$. The lowest solubility was recorded at $pH\;4{\sim}5$, whereas the best buffering action was in the range of $5.5{\sim}7.5$. On the other hand, gelation of MPI was found to depend on the protein concentration. In the cases of foamability, % volume increase and specific volume were higher for 10 min. with a good whipping ability. And also, the MPI properties of two varieties of SH and C were compared and discussed.

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A Study on a Habitat View of Korean Traditional Villages - in the case of Chung Jae Kwon Bul family - (조선시대 전통마을의 서식관에 관한 연구 - 충재 권벌의 종가를 중심으로 -)

  • 최기수
    • Journal of the Korean Institute of Landscape Architecture
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    • v.26 no.4
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    • pp.82-94
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    • 1999
  • According to Korean's geographical features of Taek Li Ji, the best location for human settlements is on the vicinity of a stream among a stream, an river and a sea, and not far from a ridge. The researcher chose one of best village which is called Yougokmaeul in Bonghwa-Gun, Kyungsang-Do. This village was created a gathering village with the same surname, can be translated as a hen and the front mountain can be seen as a rooster in the view of the shape of geographical features. As it were, the shape of this village and the cultivated land seems to be an egg inside a nest of a parent's chicken in the respect of the analogy of the theory of P'ungsuchiri which is known as Feng Shui in China and geomancy in the western world, and to effect the defensive psychology of the living environment in the terrain inland. This village is the studying place, Suckchunjungsa as the lecturing and studying place for their following students within the Chunghadonchun, the studying Yougok village, and even the ritual place performing ancestral sacrifices and the first incoming ancestor's graves in the same geographical system. The house site of the first incoming resident is surrounded the countian and is located in the front of Backsulryount which is the main mountain through Moonsu-san in the branch mountain of Korean's mountain system. Backsulryoung which is to be seen as the white peak, is symbolized the head of a hen which is to relate to a mysterious turtle in the view of P'ungsuchiri. And the pavilion which is called Chungamjung is sited on the rock of a shape of turtle which is symbolized to live longer. In the section of the mountain and water, Lee Jung Hwan mentioned a living place near the mountain stream is the best residential area and a landscape which is composed by a stream between mountains make a pleasure spirit and a bright feeling and make a refined person. If one can reach in the graceful mountain make a pleasure split and a bright feeling and make a refined person. If one can reach in the graceful mountain half day away, this kind of place must be a best living residential area to live. But this village was structured all in one place. And one of the ideas tangibly reflected in traditional Korean society's view of life and nature is the seclusion based on the Taoism. This kind of a dreamy thoughts make a dream to keep the paradise in our ancestors' mind. This kind of utopia is Chunghadonchun which is structured 5 aspects from the utopian gate near the Samgaesuewon to the village. These 5aspects is expressed by some Confucian thoughts as a small cosmos individually. On the third aspects which is the center among these aspects, the Suckchunjungsa which was made a seclusion place to devoting himself to his studies with refined tastes. The word of Jiju-am, Gangpung-dae, Jaewol-dae and Biryoung-pock are all expressed to cultivate one's moral character and to seek the truth by the Confucius'theory through the nature.

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