• 디지털콘텐츠학회 논문지
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• 제18권7호
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• pp.1303-1312
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• 2017

이데올로기들은 영화를 구성하는 다양한 요소들에 의해 표출된다. 그 중 하나가 의상이다. 본 연구는 웨스 앤더슨의 영화 작품속 의상에 표출된 유니폼의 조형적 특징과 이미지의 분석에 연구의 목적을 두고 있다. 이를 위해 논자는 2010년 이후에 제작된 웨스 앤더슨의 작품들 중 <문라이즈 킹덤>(2012), <그랜드 부다페스트 호텔>(2014)의 두 편을 연구 대상으로 선정하였다. 본 연구를 통해 유니폼과 이데올로기 양자간의 인과성과 그것이 갖는 상징적 의미를 파악할 수 있었다. 영화에 등장한 유니폼은 인물들의 직업이나 역할을 드러내기 위한 목적에서 나아가 소속감, 독립성, 권위, 사명감, 정체성과 동일체성, 자부심 등을 표출하고 있는데, 이 중 두 영화에 공통적으로 나타난 가치는 소속감, 독립성과 권위, 사명감 등이다.

• 건축역사연구
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• 제17권4호
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• pp.97-112
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• 2008

In this study, I attempted to the architectural design characteristics of Daeung-Jeon in Sudeok Temple. For this purpose, After I set up several assumptions in the basis of the general characteristics of Korean wood architecture, and then, analyzed floor plan, structural formation and section sizes of structure and bracket members in relation to module and unit. As the results, the characteristics of the design process of plan and structure are follows. (1) 1 ja(尺), the unit applied to this building is measured $307.6{\sim}318.3mm$) and the average is 312.9mm (2) It is estimated that the floor plan designed on the basis of the top of columns. By the applied unit, every bay of the front side and the side is each designed by 15 ja and 8.5 ja. (3) The section is composed of piled members which have same section size. As basic module of section size called 'jae(재;材)', it is estimated at width 0.45 ja by height 0.75 ja. And as the secondary module, height between jae and is called 'gyoe(계;)' and it wes designed by three height size of 0.25 ja, 0.27 ja and 0.30 ja, (4) It is estimated that the section plan was designed by the order as follows. Firstly, the horizontal position of purlins wes decided on the basis of the intersection point of long and short rafters, and then the position and the section size of purlins and jangheyo(長舌) wes decided on the basis of the slope of roof and rafters. Secondly, going down from purlins, the members of structure composed of 'jae' and 'gyoe' was repeated. Lastly, for the purpose of linking the structure members located on the center line of adjacent purlins organically, the height of whaban(화반) was controlled.

• BMB Reports
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• 제54권7호
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• pp.386-391
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• 2021

Owing to rapid advancements in NGS (next generation sequencing), genomic alteration is now considered an essential predictive biomarkers that impact the treatment decision in many cases of cancer. Among the various predictive biomarkers, tumor mutation burden (TMB) was identified by NGS and was considered to be useful in predicting a clinical response in cancer cases treated by immunotherapy. In this study, we directly compared the lab-developed-test (LDT) results by target sequencing panel, K-MASTER panel v3.0 and whole-exome sequencing (WES) to evaluate the concordance of TMB. As an initial step, the reference materials (n = 3) with known TMB status were used as an exploratory test. To validate and evaluate TMB, we used one hundred samples that were acquired from surgically resected tissues of non-small cell lung cancer (NSCLC) patients. The TMB of each sample was tested by using both LDT and WES methods, which extracted the DNA from samples at the same time. In addition, we evaluated the impact of capture region, which might lead to different values of TMB; the evaluation of capture region was based on the size of NGS and target sequencing panels. In this pilot study, TMB was evaluated by LDT and WES by using duplicated reference samples; the results of TMB showed high concordance rate (R² = 0.887). This was also reflected in clinical samples (n = 100), which showed R² of 0.71. The difference between the coding sequence ratio (3.49%) and the ratio of mutations (4.8%) indicated that the LDT panel identified a relatively higher number of mutations. It was feasible to calculate TMB with LDT panel, which can be useful in clinical practice. Furthermore, a customized approach must be developed for calculating TMB, which differs according to cancer types and specific clinical settings.

• Korean Journal of Radiology
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• 제23권1호
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• pp.101-111
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• 2022

Objective: Familial intracranial aneurysms (FIAs) are found in approximately 6%-20% of patients with intracranial aneurysms (IAs), suggesting that genetic predisposition likely plays a role in its pathogenesis. The aim of this study was to identify possible IA-associated variants using whole exome sequencing (WES) in selected Korean families with FIA. Materials and Methods: Among the 26 families in our institutional database with two or more IA-affected first-degree relatives, three families that were genetically enriched (multiple, early onset, or common site involvement within the families) for IA were selected for WES. Filtering strategies, including a family-based approach and knowledge-based prioritization, were applied to derive possible IA-associated variants from the families. A chromosomal microarray was performed to detect relatively large chromosomal abnormalities. Results: Thirteen individuals from the three families were sequenced, of whom seven had IAs. We noted three rare, potentially deleterious variants (PLOD3 c.1315G>A, NTM c.968C>T, and CHST14 c.58C>T), which are the most promising candidates among the 11 potential IA-associated variants considering gene-phenotype relationships, gene function, co-segregation, and variant pathogenicity. Microarray analysis did not reveal any significant copy number variants in the families. Conclusion: Using WES, we found that rare, potentially deleterious variants in PLOD3, NTM, and CHST14 genes are likely responsible for the subsets of FIAs in a cohort of Korean families.

• 한국식품영양학회지
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• 제17권2호
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• pp.163-170
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• 2004

계란찜 제조시 물의 첨가량,가열 온도와 시간, pH, NaCl의 첨가량이 전란액의 점도와 색, 계란찜의 텍스쳐와 색에 미치는 영향을 조사하였다. 계란찜은 계란을 할난시켜 완전히 섞어 계란액을 만든 다음ultrasonicator로 탈기시키고 10$0^{\circ}C$의 증기로 7분간 가열한 다음 22$^{\circ}C$에서 90분간 냉각시켰다. 그 결과 물의 첨가량은 50% 이상부터 계란액의 점도와 계란찜의 견고성 및 항복응력에 현저한 감소가 있었다. 계란액의 색은 a와 b값의 감소가 현저하였고, 계란찜은 ( - )값 범위에서의 a값이 더 낮아져 녹색이 짙어짐을 보였다. 가열 온도의 증가는 응고시간의 단축과 높은 항복응력이 측정되었으나 변형도는 감소함을 보였다. 가열시간에 따른 계란찜의 L과 b값은 9$0^{\circ}C$와 10$0^{\circ}C$에서 감소함을 보였고, a값은 8$0^{\circ}C$에서 감소함을 보였다. 소금의 첨가를 1.3%까지 하였을 때 계란찜의 항복응력과 단단함이 크게 증가하였지만, 색은 약간의 b값 감소 외에는 큰 차이가 없었다. 계란액의 pH를 4.0에서 10.0으로 증가시켰을 때 pH 6.0에서 점도가 가장 낮았으나 계란찜은 항복응력과 단단함에서 가장 높은 값을 보여주었고 pH 8.0 이상에서는 L값과 b값의 감소가 현저하였다. 이상의 결과에서 물의 첨가량과 가열온도, 가열시간, 소금첨가량은 계란찜의 텍스쳐 및 색특성에 영향을 줌이 밝혀졌다.

• 대한가정학회지
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• 제45권2호
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• pp.133-145
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• 2007

The purpose of this research was to identify the importance of emotional development in early childhood, in children ages three to five, by examining the relationship between the variables in the children such as gender, age, and temperament, as well as their mothers' emotionality, in relation to emotional development. The participants included a total of 72 children between three and five years of age. The major findings are as follow: First, there were significant differences in emotional expression and emotional recognition between the boys and the girls. Additionally, the emotional recognition of the children increased as age increased, and more positive strategies for emotional regulation were used with the increasing age of the children. Temperament characteristics did not have any relationship with emotional expression or emotional recognition, while the strategies for emotional regulation were related to the temperament characteristics. Second, the emotional expressivity of the mother was related to the emotional expression and recognition of the child, but wes not associated with strategies for emotional regulation. The emotional reactivity of the mother was related to a child's strategies for emotional regulation, but not to emotional expression or recognition. Third, emotional development of the children wes influenced by the individual child variables and emotionality of the mother.

• Clinical and Experimental Pediatrics
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• 제63권6호
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• pp.195-202
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• 2020

Developments in next-generation sequencing (NGS) techogies have assisted in clarifying the diagnosis and treatment of developmental delay/intellectual disability (DD/ID) via molecular genetic testing. Advances in DNA sequencing technology have not only allowed the evolution of targeted panels but also, and more currently enabled genome-wide analyses to progress from research era to clinical practice. Broad acceptance of accuracy-guided targeted gene panel, whole-exome sequencing (WES), and whole-genome sequencing (WGS) for DD/ID need prospective analyses of the increasing cost-effectiveness versus conventional genetic testing. Choosing the appropriate sequencing method requires individual planning. Data are required to guide best-practice recommendations for genomic testing, regarding various clinical phenotypes in an etiologic approach. Targeted panel testing may be recommended as a firsttier testing approach for children with DD/ID. Family-based trio testing by WES/WGS can be used as a second test for DD/ID in undiagnosed children who previously tested negative on a targeted panel. The role of NGS in molecular diagnostics, treatment, prediction of prognosis will continue to increase further in the coming years. Given the rapid pace of changes in the past 10 years, all medical providers should be aware of the changes in the transformative genetics field.

• BMB Reports
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• 제51권11호
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• pp.584-589
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• 2018

Secondary prevention via earlier detection would afford the greatest chance for a cure in premalignant lesions. We investigated the exomic profiles of non-malignant and malignant changes in head and neck squamous cell carcinoma (HNSCC) and the genomic blueprint of human papillomavirus (HPV)-driven carcinogenesis in oropharyngeal squamous cell carcinoma (OPSCC). Whole-exome (WES) and whole-genome (WGS) sequencing were performed on peripheral blood and adjacent non-tumor and tumor specimens obtained from eight Korean HNSCC patients from 2013 to 2015. Next-generation sequencing yielded an average coverage of $94.3{\times}$ for WES and $35.3{\times}$ for WGS. In comparative genomic analysis of non-tumor and tumor tissue pairs, we were unable to identify common cancer-associated early mutations and copy number alterations (CNA) except in one pair. Interestingly, in this case, we observed that non-tumor tonsillar crypts adjacent to HPV-positive OPSCC appeared normal under a microscope; however, this tissue also showed weak p16 expression. WGS revealed the infection and integration of high-risk type HPV16 in this tissue as well as in the matched tumor. Furthermore, WES identified shared and tumor-specific genomic alterations for this pair. Clonal analysis enabled us to infer the process by which this transitional crypt epithelium (TrCE) evolved into a tumor; this evolution was accompanied by the subsequent accumulation of genomic alterations, including an ERBB3 mutation and large-scale CNAs, such as 3q27-qter amplification and 9p deletion. We suggest that HPV16-driven OPSCC carcinogenesis is a stepwise evolutionary process that is consistent with a multistep carcinogenesis model. Our results highlight the carcinogenic changes driven by HPV16 infection and provide a basis for the secondary prevention of OPSCC.

• 생명과학회지
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• 제24권3호
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• pp.311-317
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• 2014

Rimed vacuole을 가진 원위 근육병(distal myopathy with rimmed vacuoles, DMRV)은 제2형 유전성 봉입체 근육병으로도 불리며 초기 성인기에 발병하여 원위부의 근력약화를 보이는 임상양상과 rimmed vacuole의 근육병리소견을 특징으로 하는 상염색체 열성의 근육병이다. 이러한 DMRV의 원인은 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) 유전자의 돌연변이임이 밝혀져 있다. 저자들은 원위부 근력약화를 호소하는 환자에서 전체 엑솜 염기서열분석을 이용하여 GNE 유전자의 복합 이형접합성 돌연변이(p.Asp176Val 및 p.Val572Leu)를 확인하여 DMRV를 진단할 수 있었다. 본 연구는 근육병의 정확한 분자진단에 있어서 전체 엑솜 염기서열분석의 유용성을 보여주었기에 이를 보고하는 바이다.

• 한국재료학회지
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• 제26권4호
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• pp.194-199
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• 2016

We prepared polymethyl methacrylate (PMMA) beads with a particle size of 80 nm to improve the energy conversion efficiency (ECE) by increasing the effective surface area and the dye absorption ability of the working electrodes (WEs) in a dye sensitized solar cell (DSSC). We prepared the $TiO_2$ layer with PMMA beads of 0.0~1.0 wt%; then, finally, a DSSC with $0.45cm^2$ active area was obtained. Optical microscopy, transmission electron microscopy, field emission scanning electron microscopy, and atomic force microscopy were used to characterize the microstructure of the $TiO_2$ layer with PMMA. UV-VIS-NIR was used to determine the optical absorbance of the WEs with PMMA. A solar simulator and a potentiostat were used to determine the photovoltaic properties of the PMMA-added DSSC. Analysis of the microstructure showed that pores of 200 nm were formed by the decomposition of PMMA. Also, root mean square values linearly increased as more PMMA was added. The absorbance in the visible light regime was found to increase as the degree of PMMA dispersion increased. The ECE increased from 4.91% to 5.35% when the amount of PMMA beads added was increased from 0.0 to 0.4 wt%. However, the ECE decreased when more than 0.6 wt% of PMMA was added. Thus, adding a proper amount of PMMA to the $TiO_2$ layer was determined to be an effective method for improving the ECE of a DSSC.