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Desalinated underground seawater of Jeju Island (Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t-BHP treated HepG2 cells

  • Noh, Jung-Ran;Gang, Gil-Tae;Kim, Yong-Hoon;Yang, Keum-Jin;Lee, Chul-Ho;Na, O-Su;Kim, Gi-Ju;Oh, Won-Keun;Lee, Young-Don
    • Nutrition Research and Practice
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    • v.4 no.1
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    • pp.3-10
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    • 2010
  • This study was performed to investigate the effect of desalinated underground seawater (named as 'magma seawater', MSW) of Jeju Island in Korea on lipid metabolism and antioxidant activity. MSW was collected from underground of Han-Dong in Jeju Island, and freely given to high fat diet (HFD)-fed C57BL/6 mice for 10 weeks. Although there were no significant differences in the body weight changes and plasma lipid levels, hepatic triglyceride levels were significantly lower in the MSW group than in the normal tap water (TW)-drunken control group. Furthermore, the activity of fatty acid synthase (FAS) was significantly decreased and carnitine palmitoyltransferase (CPT) activity was increased in MSW group compared to TW group. Similarly, real-time PCR analysis revealed that mRNA expressions of lipogenic genes were lowered in MSW groups compared to the control group. In a morphometric observation on the liver tissue, accumulation of fats was remarkably reduced in MSW group. Meanwhile, in vitro assay, tree radical scavenging activity measured by using diphenylpicrylhydrazyl (DPPH) was increased in MSW group. The 2'-7'-dichlorofluorescein diacetate (DCF-DA) staining followed with fluorescent microscopy showed a low intensity of fluorescence in MSW-treated HepG2 cells, compared to TW-treated HepG2 cells, which indicated that the production of reactive oxygen species by tert-butyl hydroperoxide (t-BHP) in HepG2 cells was decreased by MSW treatment. The antioxidant effect of MSW on t-BHP-induced oxidative stress in HepG2 cells was supported by the increased activities of intracellular antioxidant enzymes such as catalase and glutathione reductase. From these results, we speculate that MSW has an inhibitory effect on lipogenesis in liver and might play a protective role against cell damage by t-BHP-induced oxidative stress.

WELD REPAIR OF GAS TURBINE HOT END COMPONENTS

  • Chaturvedi, M.C.;Yu, X.H.;Richards, N.L.
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.235-243
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    • 2002
  • Ni-base superalloys are used extensively in industry, both in aeroengines and land based turbines. About 60% by weight of most modern gas turbine engine structural components are made of Ni-base superalloys. To satisfy practical demands, the efficiency of gas turbine engines has been steadily and systematically increased by design modifications to handle higher turbine inlet or firing temperatures. However, the increase in operating temperatures has lead to a decrease in the life of components and increase in costs of replacement. Moreover, around 80% of the large frame size industrial/utility gas turbines operating in the world today were installed in the mid-sixties to early seventies and are now 25 to 30 years old. Consequently, there are greater opportunities now to repair and refurbish the older models. Basically, there are two major factors influencing the weldability of the cast alloys: strain-age cracking and liquation cracking. Susceptibility to strain-age cracking is due to the total Ti plus AI content of the alloy; Liquation cracking is due either to the presence of low melting constituents or constitutional liquation of constituents. Though Rene 41 superalloy has 4.5wt.% total Ti and Al content and falls just below the safe limit proposed by Prager et al., controlled grain size and special heat treatments are needed to obtain crack-free welds. Varying heat treatments and filler materials were used in a laboratory study, then the actual welding of service parts was carried out to verity the possibility of crack-tree weld of components fabricated from Rene 41 superalloy. The microstructural observations indicated that there were two kinds of carbides in the FCC matrix. MC carbides were located along the grain boundaries, while M$_{23}$C$_{6}$ carbide was located both inter and intra granularly. Two kinds of filler materials, Rene 41 and Hastelloy X were used to gas tungsten arc weld a patch into the sheet metal, along with varying pre-weld heat treatments. The microstructure, hardness and tensile tests were determined. The service distressed parts were categorized into three classes: with large cracks, with medium cracks and with small or no visible cracks. No significant difference in microstructure among the specimens was observed. Specimens were cut from the corner and the straight edge of the patch repair, away from the corner. The only cracks present were found to be associated with inadequate surface preparation to remove oxidation. Guidelines for oxide removal and the welding procedures developed in the research enabled crack-free welds to be produced.d.

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Effect of SCB Liquid Manure Application in Pear Orchard Managed by Cover Crop System on Tree Growth, Potential Nutrient Recovery and Soil Physicochemical Properties (녹비작물 재배 시 SCB 액비 혼용이 배나무 생육 및 양분 환원 가능량, 토양 물리화학성에 미치는 영향)

  • Lee, Seong-Eun;Park, Jin-Myeon;Choi, Dong-Geun
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.779-786
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    • 2012
  • Many farmers have been seeking alternatives to chemical fertilizer for successful organic fruit production. This experiment was carried out to investigate the replaceability of chemical fertilizer by cover crop and slurry composting biofiltration (SCB) liquid manure (LM) application in pear orchard. Three treatments were contained in this experiment; cover crop only and cover crop + LM treatment, and control (chemical fertilizer application). Dry weight and mineral contents of gramineous cover crops were significantly increased in LM-combined treatments than that of leguminous species. Bulk density of soil was decreased in rye + LM and hairy vetch + LM treatments, compared with each cover crop treatment. Soil pH was lowest in fertilizer treatment and soil nitrate content became similar between treatments after rainy season. Available soil phosphate was lower in cover crop and cover crop + LM treatmemts than control, but exchangeable Mg was higher. The mineral content and net assimilation rate of leaves showed no difference between the treatments. As a result, it is suggested that the application of SCB liquid manure in pear orchard managed by cover crops is desirable to maintain the productivity by improving soil physical properties and potential nutrient recovery.

Early Autumn Maturing Pear Cultivar 'Sinhwa' with Fascinating Very Soft Flesh (부드러운 육질이 매력적인 중생종 배 '신화')

  • Kang, Sam-Seok;Kim, Yoon-Kyeong;Hwang, Hea Seong;Cho, Kwang-Sik;Shin, Il-Sheob;Won, Kyeong-Ho;Choi, Jang-Jeon;Kim, Ki-Hong;Jo, Ji Hyeong
    • Horticultural Science & Technology
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    • v.31 no.4
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    • pp.512-516
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    • 2013
  • Pear cultivar 'Sinhwa' (Pyrus pyrifolia var. culta Nakai) was originated from a cross between 'Niitaka' and Whasan' with the aims of improving the fruit quality and the traits of cultivation and of early maturing more than 'Whasan' cultivar at Pear Research Station of National Institute of Horticultural & Herbal Science, Rural Development Administration in 1995. 'Sinhwa' was preliminarily selected in 2004 and named in 2009. The tree shows vigorous growth habit and semi-spread characters like 'Niitaka'. Furthermore, it has a sufficient and well upkeep of the flower bud, so it can be more easily cultivated in orchards. In the flower characteristics, flowering time of 'Sinhwa' is $11^{th}$ April like as maternal parent 'Niitaka'. Also 'Sinhwa' has short of pollen grains, so it is need above two pollinizer cultivars. 'Sinhwa' is highly resistant to black leaf spot (Alternaria kikuchiana) and relatively strong to pear scab (Venturia nashicola) in field condition. The optimum harvest time is around Sep. $15^{th}$ in Naju, which is ahead of 'Whasan' about 10 days in the harvest period. The fruit shape is oblate and fruit skin color is yellowish-brown during harvesting time. The average weight of fruit is 627 g, and the soluble solids content is $13.0^{\circ}Brix$. The flesh is very soft and juicy, and renders good eating quality. Shelf life is about 30 days under the room temperature condition.

Rhus verniciflua Extract Ingestion and Exercise Training on Blood Lipids and Insulin Resistance in Rats (옻나무 추출액 섭취와 운동훈련이 흰쥐의 혈중 지질 및 인슐린 저항성에 미치는 영향)

  • Lee, Youn-Kyung;Lee, Soo-Chun;Jeon, Byung-Duk;Kim, Sea-Hyun;Kim, Pan-Gi;Ryu, Sungpil
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.681-689
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    • 2009
  • We investigated the effect of Rhus verniciflua Stokes extract ingestion with exercise training on plasma lipids and insulin resistance for 8 weeks. Forty SD (Sprague-Dawley) male rats were used as the experimental animals that were divided into CON (control), RVS (Rhus verniciflua Stokes), EXE (exercise training), and RVS-EXE (Rhus verniciflua Stokes with exercise training), respectively. Body weight gain in EXE (202%) and RVS-EXE (203%) was significantly lower than CON (253%) and RVS (239). Stored fats were significantly lower with RVS and/or exercise training. Blood lipids were enhanced in RVS and RVS-EXE compared to CON. Blood glucose was significantly high in CON compared to the other groups. Insulin and HOMA index has the same tendencies with glucose, however, the synergic effect was found in RVS-EXE. In conclusion, Rhus verniciflua Stokes extract ingestion with exercise training has the effect of lowering amount of stored fats, reducing blood lipids, and enhancing insulin resistance, therefore, metabolic syndrome, diabetes, obesity, and hyperlipidemia might be prevented.

Selection of Crabapple Pollinizers for 'Fuji' Apple through Physiological and Genetic Analysis (꽃사과 품종의 생리 및 유전적 분석을 통한 '후지' 사과의 수분수 선발)

  • Son, KwangMin;Choi, Dong Geun;Kwon, Soon-Il;Kim, Byung Oh;Choi, Cheol;Kang, In-Kyu
    • Journal of Bio-Environment Control
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    • v.22 no.2
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    • pp.116-122
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    • 2013
  • We investigated characteristics and self-incompatibility genotypes of 11 crabapple cultivars to introduce a new pollinizer of 'Fuji' apple tree in Korea. Flowering dates of eleven crabapples were two to seven days earlier than that of 'Fuji'. The rate of pollen germination in vitro was ranged from 85.6% to 98.0% except 'Virginia'. Controlled pollination treatment with each crabapples to 'Fuji' increased fruit set rate about 20.4% to 34.4%, the number of seed per fruit about 13.8% to 42.3% and fruit weight about 7.4% to 16.7% compared to open pollination. Tested crabapples were resistant to peach fruit moth, brown leaf spot and sooty blotch in general. A PCR amplification method using S-RNase primers was carry out in eleven crabapples. S-alleles, $S_3$, $S_5$, $S_9$, $S_{10}$, $S_{20}$, $S_{26} from six crabapples were determinated. Through sequencing analysis, $S_5$ ('Manchurian', 'Virginia') and $S_9$ ('Yantaishagou') showed 100% homologous to previous result. Based on our results, it was recommended that 'Manchurian', 'Hopa A', 'Hanyaehanakaidou', 'Spectabilis' could be promising pollinzers for 'Fuji' apple cultivar.

Fruit Productivity and Cold Hardiness as Affected by Fruit Setting of 'Wonhwang' Pear Trees ('원황' 배의 착과량 조절이 과실생산성과 내한성에 미치는 영향)

  • Kim, Byeong-Sam;Cho, Kyung-Chul;Ma, Kyung-Chul;Yun, Bong-Ki;Jung, Seok-Kyu;Choi, Hyun-Sug
    • Journal of Bio-Environment Control
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    • v.26 no.2
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    • pp.64-71
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    • 2017
  • The study was performed on the effects of crop load of pear (Pyrus pyrifolia Nakai) trees on shoot growth, reserved nutrients, and fruit production for two years, as well as regrowth of cuttings treated with various cold temperatures in 2012 and 2013. Crop load adjustments included three levels of fruiting; 54 (60%), 90 (100%), and 126 fruits per tree (140%). As increasing crop load from 60% to 140%, water sprout growth decreased, and one-year old shoot resulted in declined concentrations of total carbon, macro-nutrients, and free sugar. The 140% of crop load greatly increased fruit yield of approximately 35 tons per ha in both 2011 and 2012 but slightly reduced weight, size, and soluble solid contents in fruit. However, 140% of crop load increased annual production income to approximately over 10 million won compared to the 60% and 100% of crop load. Germination rates in the cuttings did not rapidly decrease up to $-20^{\circ}C$ in a growth chamber condition. The 140% of crop load decreased germination rates less than 70% of cuttings grown under $-30^{\circ}C$. Amounts of electrolyte leakage in the cuttings under various cold temperatures were not significantly different among the adjustment of crop load.

Studies on the Effects of Black-tipped Sawfly, Acantholyda posticalis posticalis Matsumura on the Growth of the Korean White Pine, Pinus koraiensis S. et Z. (잣나무넓적잎벌 피해(被害)가 잣나무생장(生長)에 미치는 영향(影響)에 관(關)한 연구(硏究))

  • Chung, Sang Bae;Shin, Shang Chul
    • Journal of Korean Society of Forest Science
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    • v.83 no.4
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    • pp.450-459
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    • 1994
  • The damage by the black-tipped sawfly, Acantholyda posticalis posticalis Matsumura, has been increasing at several locations in central part of the Korean peninsula. This study was undertaken at selected plots in Kapyung-gun, Kyunggi Province and Hongchon-gun, Kangwon Province from 1984 to 1987. The purpose of this study was to provide basic information for integrated control of this pest and to determine the economic threshold by investigating the pest host interactions with special reference to damage analysis. The results obtained were as follows ; 1. The damage was more severe at upper part than at lower part of the crown. The vertical distribution of dry weight of damaged trees above ground showed L-type distribution, whereas that of healthy trees showed C-type distribution. 2. The needle loss by sawfly reduced both of height and the diameter growth of the main stem. The growth reduction occurs when the needle loss is 50% or higher. About 10% of tree growth was reduced at 50% of needle loss. 3. The reduction of diameter growth was more severe at upper part than at lower part of the main stem. 4. Volume growth was reduced when more than 50% of the needles were damaged after three years of the sawfly infestation. Reductions of total volume increment at the levels 70% and 90% of needle damage were 9% and 20%, respectively.

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Studies on Charateristics of Pinus densiflora Forest in Kangwon Province (II). Constructive and Maintenance Respiration as Related to Growth of Saplings (강원도(江原道) 소나무림(林)의 특성(特性)에 관한 종합적(綜合的) 연구(硏究)(II) 유령목(幼齡木)의 생장(生長)에 따른 구성호흡(構成呼吸)과 유지호흡(維持呼吸))

  • Han, Sang Sup;Chang, Chun Geun;Kim, Sun Hee
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.221-228
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    • 1994
  • Respitation and growth rates of leaves, branches, stems and roots of 2 to 11-yr-old red pine trees(Pinus densiflora) were examined and applied to Thornley's growth equation, $$R=(\frac{1-Yg}{Yg})\frac{dW}{dt}+mW$$. The conversion efficiency of substrates(Yg), maintenance respiration coefficients(m), relative growth rates(${\mu}$) were estimated. The efficiency of conversion of substrates (Yg) was 0.3637g/g dw/yr and the maintenance respiration coefficient(m0 was 0.094g/g dw/yr. The relative growth rate(${\mu}$) was remarkably reduced with age from 0.90(2-year-old) to 0.33:11 year-old). The Ratio of gross respiration(R) per gross photosynthesis(Pg), R/Pg showed the range of 0.6~0.7 and annually 64% of Pg was spent for constructive respiration. The 3.4% of dry weight of whole tree was spent for maintenance respiration.

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Use of Sprinkler System for Production Forest Management of Pine Mushroom (Tricholoma matsutake) (살수장치(撒水裝置)를 이용(利用)한 송이산 관리(管理)에 관(關)한 연구(硏究))

  • Chung, Sang Bae;Kim, Chul Su
    • Journal of Korean Society of Forest Science
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    • v.94 no.1 s.158
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    • pp.21-25
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    • 2005
  • In order to prevent the pine mushrooms, Tricholoma matsutake, from being damaged by the pine needle gall midges, Thecodiplosis japonensis, and thereby, to increase their production and improve their quality, a sprinkler system was installed on the mushroom field. A low-concentration insecticide (deltamethrin 1% EC, x2,000) was sprayed once at insects' most active time every day during the period of insects' adult occurrence and thereafter, the irrigation by ground water spraying was periodically enforced. Such a test was conducted at Yangyang-Gun, Kwangwon-do, Korea for 2 years from 2000 through 2001. The pine needle gall midges generally emerged for about 40 days from late May to early July. 50% emergence of them was about June 6, and peak emergence (more than 80%) was early or mid-June. Gall formation rate was 3.5% on average with this ground insecticide spraying, while 51.3% when not treated. Control effectiveness of this insecticide spraying was 92.3%, which was higher than 82.5% by the conventional injection of insecticide into tree stems. Pine mushrooms emerged for about 35 days from mid-September through earlier October, and around 80% of them did for about 15 days from late September through early October. As a result of the periodic ground water-spraying (30 mm per week) for 2 months (from August to October), the production of mushrooms increased by 74.3% (110% in terms of weight), with their quality improvement. The mushrooms produced from the treated stand by the spraying system were priced 8,670,000 wons per hectare, and thus, the net income deducting the facility and management cost was 4,310,000 wons, about 5% higher than value from the control stand. It was analyzed that this treatment was significantly cost effective when the facilities are used more than 5 years.