• 한국식품위생안전성학회지
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• 제38권3호
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• pp.158-163
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• 2023

본 연구에서는 경기도내 유통되고 있는 심해성 어류 50건을 대상으로 수은, 메틸수은의 오염도 및 셀레늄의 함량을 알아보았다. 금아말감법의 수은분석기, ICP-MS를 사용하여 측정하였으며 각 항목별 평균함량은 수은 0.7647 mg/kg (0.0182-5.3620), 메틸수은 0.0764 mg/kg (0.0096-0.8750), 셀레늄 0.4728 mg/kg(0.1075-3.5100)으로 메틸수은은 50건 모두 기준규격(1.0 mg/kg) 이하로 나타났다. 셀레늄은 수은과 결합하여 수은의 독성을 감소시키는 것으로 알려져 있으며, 본 연구과제에서는 일일평균섭취량이 3.3 ㎍/kg으로 일일권장량보다 낮은 수치였다. 유해물질인 수은과 메틸수은은 JECFA에서 설정한 PTWI의 5.7%, 1.8%로 조사되었다. 따라서 도내에서 유통되는 심해성 어류의 수은, 메틸수은과 같은 위해중금속은 안전한 것으로 나타났지만 지속적 모니터링이 필요하다고 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1262-1265
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• 2006

The present study was undertaken to analyze the status of erythrocytic malonyl dialdehyde (MDA) as an index of lipid peroxidation in normal cycling and ${\alpha}$-tocopherol supplemented anestrus buffalo heifers. Before supplementation, the blood samples were collected at weekly interval in anestrus and normal cycling buffalo heifers and data from four samples was pooled to establish pre-supplementation base line. Thereafter, the blood samples from anestrus and ${\alpha}$-tocopherol supplemented anestrus buffalo heifers were taken at fortnightly interval for 12 weeks during supplementation and for four weeks during the post-supplementation period. The pre-supplementation endogenous erythrocytic MDA, 0.3% $H_2O_2$ induced-erythrocytic MDA and 1.5% $H_2O_2$ induced-erythrocytic MDA levels (nmol/g Hb) increased significantly (p<0.05) in anestrus heifers ($372.3{\pm}5.0$, $564.4{\pm}5.7$, $862.4{\pm}8.5$) when compared to normal cycling animals ($289.6{\pm}3.0$, $508.2{\pm}6.0$, $777.7{\pm}4.9$). Oral supplementation of 3,000 mg of ${\alpha}$-tocopherol per week per animal in anestrus heifers resulted in a progressive and significant (p<0.01) decline in erythrocytic MDA levels. These observations implied that supplementation of ${\alpha}$-tocopherol to anestrus buffalo heifers ameliorated their antioxidant status by alleviating the effects of oxidative stress.

• Animal Bioscience
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• 제34권1호
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• pp.26-35
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• 2021

Objective: This study was conducted to assess the level of pregnancy-associated glycoprotein (PAG) in whole and skim milk samples, and its suitability for early pregnancy diagnosis in goats. Methods: A two-step sandwich enzyme-linked immunosorbent assay (ELISA) system for estimation of milk PAG was developed and validated, which employed caprine-PAG specific polyclonal antisera. Whole and skim milk samples (n = 210 each) from fifteen multiparous goats were collected on alternate days from d 10 to d 30, and thereafter weekly till d 51 post-mating. PAG levels in milk samples were estimated by ELISA and the pregnancies were confirmed at d40 post-mating by transrectal ultrasonography (TRUS). Results: The level of PAG in whole and skim milk samples of both pregnant and nonpregnant goats remained below the threshold values until d 24 after mating. Thereafter, PAG concentration in whole and skim milk increased steadily in pregnant goats, whereas it continued below the threshold in non-pregnant does. The PAG profiles in whole and skim milk of pregnant goats were almost similar and exhibited strong positive relationship (r = 0.891; p<0.001). Day 26 post-mating was identified as the first time-point for significantly (p<0.05) higher milk PAG concentration in pregnant goats than to non-pregnant goats. When compared to TRUS examination for pregnancy diagnosis, the accuracy and specificity of PAG ELISA using whole and skim milk samples were 94.5% and 95.4%; and 95.3% and 100%, respectively. The high values of area-under-curve (0.904 [whole milk] and 0.922 [skim milk]), demonstrate outstanding discrimination ability of the milk assays. Among the sampling dates chosen, d 37 post-mating was identified as the best suitable time point for collection of milk samples to detect pregnancy in goats. Conclusion: The PAG concentration in whole and skim milk of goats collected between days 26 and 51 post-breeding can be used for the accurate prediction of pregnancy and may be useful for assisting management decisions in goat flocks.

• 한국안광학회지
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• 제9권2호
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• pp.381-389
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• 2004

사용기간과 보관 온도에 따른 소프트콘택트렌즈 다목적용액의 변화를 알아보기 위해 4개 회사의 제품을 조사하였다. 실험에 사용한 다목적용액들을 각각 $4^{\circ}C$, $20^{\circ}C$ 및 $30^{\circ}C$로 보관하면서 24주간동안 단백질 제거 효능을 측정한 결과, 사용기간이나 보관용도에 따라 통계적으로 의미있는 변화가 나타나지 않았다. 다목적 용액들의 pH를 24주 동안 매주 측정한 결과, 개봉 직후 pH는 각기 7.0, 7.5, 7.6 및 8.2로 제품마다 큰 차이가 났으며 한 제품은 안자극을 유발시키지 않는 pH인 6.6~78 범위를 벗어났다. 24주간의 시험기간 동안 pH는 점점 산성화되어 24주에 이르러서는 제품들의 평균 pH는 6.6, 7.2, 7.2 및 7.7로 되어 제품에 따라서는 사용기간에 따라 역시 자극감을 느끼는 pH가 될 수 있음을 알 수 있었다. 그러나, 이러한 pH 변화는 보관온도와는 관계가 없었다. 또한 24주 동안 사용한 한 개의 다목적용액에서 세균의 오염이 관찰되었으며, 박층크로마토그라피와 UV 흡광 양상비교를 통한 성분의 변화여부 분석에서 성분의 변화가 나타남을 확인하였다. 본 연구로 다목적 용액의 변화가 보관온도와는 상관이 없었지만, 보관기간과는 큰 연관성이 있어 착용자에게 불편감을 초래할 수 있는 것으로 확인되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1761-1769
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• 2007

This experiment was conducted to determine the extent to which susceptibility to cortisol-induced follicle shutdown is influenced by fleece phentotype. Twenty Finewool (10 sheep low fibre diameter, low coefficient of fibre diameter-LL and 10 low fibre diameter, high coefficient of variation of fibre diameter-LH) and twenty Strongwool (10 low fibre diameter, low coefficient of variation of fibre diameter-HL and 10 high fibre diameter and high coefficient of variation of fibre diameter-HH) sheep of 9 months of age were individually penned in an animal house and were injected intramuscularly with an aqueous suspension of hydrocortisone acetate at a rate of 1.42 mg/kg body weight for a period of two weeks. Fibre diameter was measured from clipped tattooed patch wool samples. Follicle activity was measured by histological changes in skin biopsies taken weekly. Blood samples were collected at two-week intervals and plasma cortisol measured. Increased plasma cortisol concentration significantly (p<0.05) reduced clean wool production and mean fibre diameter dropped to its lowest level four weeks after commencement and two weeks after the cessation of cortisol injection. Elevation of plasma cortisol concentration significantly (p<0.0001) increased the percentage of inactive follicles two weeks after injection started. High fibre diameter groups (Strongwool sheep; i.e. HL+HH) had significantly (p<0.0001) higher percentage of follicle shutdown than low fibre diameter groups (Finewool sheep; i.e. LL+LH). Average percentage of shutdown follicles for Finewool (LL+LH) and Strongwool (HL+HH) Merino sheep was $9.8{\pm}0.9$ and $13.5{\pm}0.9$ respectively. Shutdown of primary follicles was more pronounced in Finewool than Strongwool sheep. There was no significant effect of coefficient of variation of fibre diameter on propensity to follicle shutdown induced by exogenous cortisol. It is concluded that elevation in plasma cortisol concentration is inhibitory to the normal activity of follicles in Strongwool sheep but that variation in fibre diameter has little or no effect.

• 대한수의학회지
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• 제36권2호
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• pp.471-482
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• 1996

These experiments were undertaken in order to find out the useful clinicopathological diagnostic methods of cadmium poisoning in dogs. Twenty-one dogs were divided into a control group and 6 experimental groups. The experimental groups were adminstered orally 5, 10, 15, 30, 60 and 120mg of cadmium per kg of body weight for 56 days. All dogs were examined for clinical signs, and weekly changes in hematological and blood chemical values. All dogs were necropsied on 57th days of experiment. Tissue samples including hair, skin, muscle, lung, liver, kidney, spleen, pancreas, testis, ovary, uterus, and bone were collected and analyzed for cadmium, zinc, iron and copper contents using atomic absorption spectrophotometer. From these experiments following results were obtained : 1. All experimental dogs showed vomitting, salivation, anorexia, decreased water-intake, dehydration, and marked weight loss. The dogs received 30mg/kg or more of cadmium died during the period from 2nd to 7th week after administration. 2. Hematologically, all experimental dogs showed decrease in erythrocyte count, hemoglobin concentration, and packed cell volume. The anemia was identified as normocytic and regenerative morphologically. 3. No significant differences in serum glutamic oxaloacetic transminase, glutamic pyruvic transaminase, blood urea nitrogen, and cholosterol value were obseved between the control and experimental dogs. 4. The cadmium contents in various tissues of experimental dogs were estimated as $37.8{\sim}201.8{\mu}g/g$ in bone, $14.1{\sim}49.5{\mu}g/g$ in liver, $13.2{\sim}53.1{\mu}g/g$ in kidney, $0.4{\sim}35.2{\mu}g/g$ in pancreas, $0.8{\sim}35.4{\mu}g/g$ in spleen, $0.9{\sim}30.1{\mu}g/g$ in hair, $0{\sim}7.1{\mu}g/g$ in lung, $0{\sim}5.1{\mu}g/g$ in skin, and $0{\sim}3.6{\mu}g/g$ in muscle, respectively. However, the serum, testis, ovary and uterus showed no cadmium accumulation. Two contol dogs showed cadmium accumulation only in bone. 5. Significant differances in zinc, iron, and copper contents in tissue samples were observed between the control and experimental groups.

• 한국대기환경학회지
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• 제16권5호
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• pp.453-467
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• 2000

The aim of this research is to collect and characterize fine particles (FPM:$\leq$2.5${\mu}{\textrm}{m}$) and coarse particles (CPM: 2.5~10${\mu}{\textrm}{m}$) using a low volume air sampler provided by the IAEA, at urban (Taejon) and rural area(Wonju) for a period of about two years(April 1996 to May 1998) and to promote a use of nuclear analytical techniques for air pollution studies. For the collection of airborne particulate matter (PM(sub)10), the Gent stacked filter unit sampler and polycarbonate membrane filters were employed. The concentration of trace elements in collected APM samples were determined byu instrumental Neutron Activation Analysis. For validation of the analytical data, internal quality control were implemented by using both the comparison of the analytical results of standard reference materials(NIST SRM 1648) and interlaboratory comparison for proficiency test (NAT-3). The standard uncertainty was less than 15% and Z-score of two samples were within $\pm$1. The monitoring of (PM(sub)10) mass concentration and elemental concentrations were carried out weekly. The average mass concentration of (PM(sub)10) in urban and rural areas were 59.2$\pm$36.5$\mu\textrm{g}$/㎥ and 41.4$\pm$23.7$\mu\textrm{g}$/㎥, respectively. To investigate the emission source, the enrichment factors were calculated for the fine and coarse particle fractions at two sites, respectively and these values were classified for anthropogenic and soil origin elements.

• Asian-Australasian Journal of Animal Sciences
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• 제26권10호
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• pp.1406-1415
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• 2013

The study was conducted to correlate the periparturient immune status in terms of neutrophil functions and cytokine expression in peripheral blood mononuclear cell culture with impending postpartum reproductive disorders in buffaloes. Forty pregnant buffaloes were observed for occurrence of postpartum reproductive disorders (PRD), i.e., metritis, endometritis and delayed uterine involution etc., during one week prepartum to four weeks postpartum period. A representative number (n = 6) of buffaloes that did not develop any PRD were included in group I (healthy, control), while the animals which experienced PRD were assigned into group II (PRD, n = 8). The blood samples were collected at weekly interval from one week prepartum to four weeks postpartum period considering the day of calving as 'd 0'. Differential leucocytes counts, superoxide and hydrogen peroxide production activity in isolated neutrophils and the mRNA expression profile of cytokines i.e., IL-2, IL-4 and IFN-${\gamma}$ in PBMC culture were studied in all the samples. A higher total leucocytes, neutrophil and band cells count along with impaired neutrophil functions i.e., lowered level of production of superoxide and hydrogen peroxide before parturition and during early postpartum period were observed in buffaloes developing PRD. Further, a lower expression of IL-2, IFN-${\gamma}$ and IL-4 mRNA in PBMC culture was observed at calving in buffaloes that subsequently developed PRD at later postpartum. Thus, suppression in neutrophil function and cytokine expression at prepartum to early postpartum period predisposes the buffaloes to develop postpartum reproductive disorders. Hence, monitoring of neutrophils function and cytokine expression profile would be effective to predict certain reproductive disorders at late pregnancy or immediately after parturition in buffaloes. In future, this may be a novel approach for determining suitable management and therapeutic decisions for prevention of commonly occurring reproductive disorders in farm animals.

• 한국수자원학회논문집
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• 제33권5호
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• pp.561-568
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• 2000

1999년 4월부터 11월까지 전남 영암의 영암호 갑문식어도에서 매주 1회씩 어도로 들어오는어류를 채집한 결과 25종 116,014개체를 채집하였으며, 이중 웅어, 줄공치, 숭어 등 10종이 하천과 바다를 오가며 서식하는 회유성어류로 전채개체수의 99.5%를 차지하였다. 회유성어류중 개체수로는 줄공치, 빙어, 웅어 숭어의 순으로 많이 채집되고 이들 4종이 전 채집 개체수의 99%에 해당하고 나머지 6종은 매우 드물게 채집되었다. 빙어는 63%가 7월 20일, 숭어는 43%가 7월 15일, 웅어는 42%가 5월 10일, 줄공치는 35%가 5월1일에 채집되어 어종별로 뚜렷한 회유시기가 있었다. 회유성 어류중 담수호에서는 웅어, 빙어, 숭어, 문절망둑 등 7종이 채집되고 유입하천에서는 은어, 빙어, 뱀장어 등 10종이 채집되어 어도를 통하여 소상한 회유성 어류가 담수호를 통하여 하천으로 올라가는 것이 확인되었다.

• 실천공학교육논문지
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• 제15권1호
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• pp.31-38
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• 2023

본 논문에서는 비전공자들을 위한 기초 머신러닝 모델 학습 및 활용교육 커리큘럼을 제안하고, Orange 머신러닝 모델 학습 및 분석 도구를 활용한 교육 방법을 제안하였다. Orange는 오픈 소스기반 머신러닝 및 데이터 시각화 도구로서, 복잡한 프로그래밍 없이 시각적인 위젯을 사용하여, 데이터를 학습시켜 머신러닝 모델을 만들 수 있다. Orange는 비전공자 학부생부터 전문가 그룹까지 다양하게 사용되는 플랫폼이다. 본 논문에서는 한 학기 분량의 기초 머신러닝 모델 학습 및 활용교육 커리큘럼과 주별 실습 내용을 제시하였다. 그리고, 머신러닝 모델 학습 및 활용에 대한 교육 내용 실체를 실증하기 위해, Orange 도구를 활용하여, 분류 데이터(Categorical Data) 표본과 수치 데이터(Numerical Data) 표본으로부터 머신러닝 모델을 학습시키고, 모델을 활용하여 모집단의 결과를 예측하는 활용 사례들을 제안하였다. 마지막으로 본 커리큘럼에 대한 교육 만족도를 비전공자 대상으로 조사 및 분석하였다.