• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1531-1541
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• 2023

Lipase is a well-known and highly in-demand enzyme. During the last decade, several lipase optimization studies have been reported. However, production costs have always been a bottleneck for commercial-scale microbial enzyme production. This research aimed to optimize the conditions for lipase production by Limtongozyma siamensis DMKU-WBL1-3 via a One-Factor-At-a-Time (OFAT) approach combined with statistical methods while using a low-cost substrate. Results suggest that low-cost substrates can be substituted for all media components. An optimal medium was found, using response surface methodology (RSM) and central composite design (CCD), to consist of 0.50% (w/v) sweet whey, 0.40% (w/v) yeast extract (food grade), and 2.50% (v/v) palm oil with the medium pH adjusted to 4 under shaking flask cultivation. From an economic point of view, this work was successful in reducing production costs while increasing lipase productivity. The medium costs were reduced by 87.5% of the original cost while lipase activity was increased by nearly 6-fold. Moreover, lipase production was further studied in a 2-L stirred-tank fermentor. Its activity was 1,055.6 ± 0.0 U/ml when aeration and agitation rates were adjusted to 1 vvm and 170 rpm, respectively. Interestingly, under this optimal lipase production, the yeast showed accumulated lipids inside the cells. The primary fatty acid is a monounsaturated fatty acid (MUFA) that is typically linked to health benefits. This study hence reveals promising lipase production and lipid accumulation by L. siamensis DMKU-WBL1-3 that are worthy of further study.

• Proceedings of the KSR Conference
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• 2004.06a
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• pp.767-772
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• 2004

A dynamic simulation program of a catenary-pantograph system including tunnel section and transient section is developed in this study. The simulation program can accommodate for the pantograph of two panheads and three d.o.f model. Using the developed program, the dynamic characteristics with a SCHUNK'S WBL 85-PANTOGRAPH is analyzed at the conventional TAEBAEK line and its tunnel section when the catenary system is supported by a tunnel bracket. The simulation results show that the variation of contact force and uplift displacement is allowable in general section and the entrance and exit of a tunnel, but the uplift displacement and the separation ratio within tunnel section is difficult to allow.

• Journal of the Korean Society for Railway
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• v.8 no.1
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• pp.51-56
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• 2005

A dynamic simulation program of a catenary-pantograph system including tunnel section and transient section is developed in this study. The simulation program can accommodate for the pantograph of two panheads and three d.o.f model. Using the developed program, the dynamic characteristics with a SCHUNK'S WBL 85-PANTOGRAPH are analyzed at the conventional TAEBAEK line and its tunnel section when the catenary system is supported by a tunnel bracket. The simulation results show that the variation of contact force md uplift displacement is allowable in general section and the entrance and exit of a tunnel, but the uplift displacement and the separation ratio within tunnel section is difficult to allow.

• Korean Journal of Food Science and Technology
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• v.50 no.6
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• pp.608-613
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• 2018

For increasing both the viscosity and intake of calories and nutrients, four cereal powders: Goami (GR), waxy barley (WBL), germinated brown rice (GBR), and oat (OT) were added to rice porridge, and the properties of the porridge were compared with that containing a commercial thickener. Among the samples, the porridge with WBL showed the highest viscosity, whereas that with GR showed the lowest. With regards to color properties, the porridge with GR showed the highest whiteness, while those with OT or GBR had the highest yellowness, affecting the color of the rice porridge. The viscosity level of porridges with cereal powders did not reach that of the porridge with the commercial thickener at $90-95^{\circ}C$; however, at $55-60^{\circ}C$, the viscosity of the porridge with cereals was enough to replace the commercial thickener. With regards to organoleptic tests, the overall acceptability was mainly attributed to color, and there were no significant differences in other properties. From these results, it seems that the cereal powders could be suitable substitutes for commercial thickeners.

• The Plant Pathology Journal
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• v.36 no.2
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• pp.185-191
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• 2020

Streptomyces griseus S4-7, a well-characterized keystone taxon among strawberry microbial communities, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the present study, we created a proteomics reference map for S. griseus S4-7. Additionally, analysis of differentially expressed proteins was performed against a wblE2 mutant, which was deficient in spore chain development and did not express an antifungal activity-regulatory transcription factor. We believe that our data provide a foundation for further in-depth studies of functional keystone taxa of the phytobiome and elucidation of the mechanisms underlying plant-microbe interactions, especially those involving the Streptomyces genus.

• Child Health Nursing Research
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• v.11 no.1
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• pp.117-124
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• 2005

Purpose: The purpose of this study was to investigate the effects of sensory stimulation conducted by primiparas on mother-infant interaction, maternal self-esteem and infant temperament. Method: A non-equivalent control group post-test study design was used. The data were collected from December 1, 2001 to March 30, 2003. The participants were 39 primiparas with normal birth weight infants. They were divided into two groups, 20 mothers in the intervention group and 19 in the control group. The mothers in the intervention group were given instruction on sensory stimulation by the researcher, and the mothers, in turn, used this technique on their infants twice a day during the first 2 months of life. Both groups were measured for maternal self-esteem and infant temperament using the maternal self-report inventory (MSRI) and What My Baby Is Like (WBL) and a film was made of the feeding situation when each infant was between 8 and 10 weeks of age. Results: Compared to the control group, the experimental group had significantly higher scores in mother-infant interaction, maternal self-concept and infant temperament. Conclusion: This suggests that sensory stimulation conducted by primiparas may improve the mother-infant interaction, maternal self-esteem and infant temperament.

• The KIPS Transactions:PartA
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• v.9A no.1
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• pp.113-120
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• 2002

Many CAI materials have been developed for middle school education. Among subjects in middle school education curricula, various multimedia materials are used in science subjects. Especially, those materials are necessary to teach basic knowledge of the environment, structure and components of animal. Thus, we implemented a multimedia title for a chapter of heart and blood circulation from first year science subject in middle school curricula. Most previous researches mainly focussed on the development of CAI programs, but did not suggest the analysis result of impact using those CAI programs. In this paper, we presented the analysis method of impact and the result, but also suggested a way to improve effectiveness of teaching in a clams.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.1
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• pp.750-753
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• 2005

Internet service, a part of our life, requires the changes not only of environment in our life but also of people's sense of value. It also requires a quick change in the spear of education activities without exception. It brought a lot of changes into educational methods, our recognition on education, and various kinds of educational tools and environment. The introduction of WBI (Web Based Instruction) is one of the most important thing. This study have applied WBI to Industrial high school education to improve its quality and accomplish its effective teaching-learning. That is, we have applied CNC(Computerized Numerical Control) and Processing learning to machining center learning and have given students chances and various learning materials so that they could contact with new information. We aimed at testing and studying some effects on the improvements of learning accomplishment and motive in this study.

• Child Health Nursing Research
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• v.12 no.3
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• pp.314-321
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• 2006

Purpose: The purposes of this descriptive survey study were to describe postpartum depression level and to identify the influence of postpartum depression on maternal role confidence, parenting stress and infant temperament, in order to develop a base for nursing intervention programs for mothers who have postpartum depression. Method: Data were collected from 183 mothers of infants who visited one public health center in Goyang city. The instrument used for this study was a self-report questionnaire to identify the postpartum depression (BDI), maternal role confidence, parenting stress (PSI) and infant temperament (WBL). Results: Of the mothers 42.1% were in the normal range for postpartum depression, 33.3% in the mild group and 24.6% were in the moderate to severe group. There were significant differences in parenting stress, and infant temperament according to level of postpartum depression. There were correlations between maternal role confidence (r=-0.13), infant temperament (r=-0.20), parenting stress (r=0.51) and postpartum depression, and weak negative correlation between parenting stress and infant temperament (r=-0.30). Conclusion: Postpartum depression in the infant period when the mother's role is very important and the effect is not only on the mother's role but also on the infant's growth. It is essential to assess and provide immediate care to mothers who have postpartum depression.

• Korean Parent-Child Health Journal
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• v.8 no.2
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• pp.123-136
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• 2005

This research is to compare the infant's temperament and parenting stress perceived by premature infants' mother and full-term infants' mother. It aims at establishing the healthy relationship between mothers and infants. It investigates the basic data of nursing intervention program to help the mother of premature infant. The period of data collection was from May 1, 2005 to May 30, 2005, and the subjects were total 123 mothers: 44 mothers of premature infants and 79 mothers of full-term infants under 6 months who visited general hospitals, individual pediatrics, and health center located in G city. "What My Baby Is Like(WBL)" developed by Pridham, Chang, and Chiu(1994) and translated by Bang Kyeong Sook(1999) was used as a measuring instrument of infants' temperament, and Parenting Stress Index (PSI) developed by Abidin(1990) and revised and complemented by Kim Dong Hee(1997) was used as a measuring instrument of parenting stress. Collected Data were analyzed through $X^2$-test, t-test, Pearson's correlation with SPSS 12.0 window program. The results are as follows: 1. There was a significant difference in the infant's temperament perceived by premature infants' mothers and full-term infants' mothers (t=-4.08, p=.00). In subcategory, there were significant difference between premature infants' mother and full-term infants' mother in geniality(t=-3.62, p=.00), adaptation(t=-3.43, p=.00) and reaction(t=-2.01, p=.05). 2. There was a significant difference in parenting stress between premature infants' mother and full-term infants' mother(t=6.57, p=.00). The parenting stress of premature infants mothers appeared to be higher than full-term infants' mothers. They showed the higher stress in the mother-child relationship area(t=6.27, p=.00) and child area(t=7.38, p=.00) among 3 areas of parenting stress. 3. There were negative correlation between infants' temperament and parenting stress. As mothers perceived the infant's temperament negatively, the parenting stress. Especially, the negative correlation of infants' temperament and parenting stress of premature infants' mothers(r=-.44) was stronger than that of full-term infants' mothers(r=-37). From the research, as mothers of premature infants receive more stress, their stress can cause the serious problem to the relationship of the mother and the infant. Therefore, the nursing intervention should be carried out in order to change the negative perception of mothers towards their infants into the positive perception reduce the parenting stress.