• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.194-194
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• 2017

Camelina (Camelina sativa L.) is a potential bio-energy crop that has short life cycle about 90 days and contains high amount of unsaturated fatty acid which is adequate to bio-diesel production. Enhancing environmental stress tolerance is a main issue to increase not only crop productivity but also big mass production. CsRCI2s (Rare Cold Inducible 2) are cold and salt stress related protein that localized at plasma membrane (PM) and assume to be membrane potential regulation factor. These proteins can be divide into C-terminal tail (CsRCI2D/E/F/G) or no-tail group (CsRCI2A/B/C/H). However, function of CsRCI2s are less understood. In this study, physiological responses and functional characterization of CsRCI2s of Camelina under salt stress were analyzed. Full-length CsRCI2s (A/B/E/F) and CsPIP2;1 sequences were confirmed from Camelina genome browser. Physiological investigations were carried out using one- or four-week-old Camelina under NaCl stress with dose and time dependent manner. Transcriptional changes of CsRCI2A/B/E/F and CsPIP2;1 were determined using qRT-PCR in one-week-old Camelina seedlings treated with NaCl. Translational changes of CsRCI2E and CsPIP2;1 were confirmed with western-blot using the antibodies. Water transport activity and membrane potential measurement were observed by cRNA injected Xenopus laevis oocyte. As results, root growth rate and physiological parameters such as stomatal conductance, chlorophyll fluorescence, and electrolyte leakage showed significant inhibition in 100 and 150 mM NaCl. Transcriptional level of CsPIP2;1 did not changed but CsRCI2s were significantly increased by NaCl concentration, however, no-tail type CsRCI2A and CsRCI2B increased earlier than tail type CsRCI2E and CsRCI2F. Translational changes of CsPIP2;1 was constitutively maintained under NaCl stress. But, accumulation of CsRCI2E significantly increased by NaCl stress. CsPIP2;1 and CsRCI2A/B/E/F co-expressed Xenopus laevis oocyte showed decreased water transport activity as 61.84, 60.30, 62.91 and 76.51 % at CsRCI2A, CsRCI2B, CsRCI2E and CsRCI2F co-expression when compare with single expression of CsPIP2;1, respectively. Moreover, oocyte membrane potential was significantly hyperpolarized by co-expression of CsRCI2s. However, higher hyperpolarized level was observed in tail-type CsRCI2E and CsRCI2F than others, especially, CsRCI2E showed highest level. It means transport of $Na^+$ ion into cell is negatively regulated by expression of CsRCI2s, and, function of C-terminal tail is might be related with $Na^+$ ion influx. In conclusion, accumulation of NaCl-induced CsRCI2 proteins are related with $Na^+$ ion exclusion and prevent water loss by CsPIP2;1 under NaCl stress.

• Korean Journal of Heritage: History & Science
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• v.51 no.3
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• pp.72-87
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• 2018

This study highlights a drainage gate and a ditch, which existed around the whole area of the western shore of Wolji Pond(月池) and focuses on a possible connection between the drainage facility on the western shore and the historical drainage system of Wolji Pond. Specifically, it primarily considered locations and the form of a drainage gate, the relationship between northwestern ditch of Wolji Pond and the drainage gate, and the establishment period and the character of the drainage facility on the western shore. The drainage gate found in excavation in 1975 is determined as the same facility as Surakgu(水落口) recorded on an actual measurement drawing, 1922. Therefore, it is highly probable that there were already the drainage facility in the western shore of Wolji Pond before the 1920s. The drainage gate constructed by processing rectangular stones has four drainage holes for controlling water level. The way of the drainage through the drainage holes is the same as that of the northern shore of Wolji Pond. From a cadastral map drawn in 1913, it is found that the ditch existed in northwest of Wolji Pond. The ditch was proximate to the drainage gate and shared the same axes. Hence, the ditch and the drainage gate are determined as a organic facility connected to the drainage system of Wolji Pond. In particular, the ditch existed in northwest of Wolji Pond is the basis for judging that the drainage facility in the western shore were established before the 1910s. Water flowed in through drainage holes of the drainage gate is drained into the northwest of Wolji Pond, through the ditch. The establishment period and the intention of the drainage facility on the western shore can be interpreted in two aspects. First, they might be 'a agricultural irrigation facility in the Joseon era', given that Wolji Pond was recorded as a agricultural reservoir, and that the whole northwestern area of Wolji Pond was used as farm land areas. Second, they might be 'a drainage facility for controlling the water level in creating Wolji Pond', given that the drainage gate was annexed to the lower shore forming the waterline of Wolji Pond, and that the hight of drainage holes on top of the drainage gate was similar to the full water level of Wolji Pond. Considering the related grounds and circumstance, the latter possibility is high.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.15 no.1
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• pp.1-10
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• 2003

In this paper, the laboratory test results with middle-sired ,soil box test in modeling the in-situ installing of horizontal drains are discussed the estimation of the optimum negative pressure. The test was carried out in the different vacuum pressure conditions together with the measurement for the settlement and volume change of drained water by the installed drains during the consolidation process. After the test, the water content was measured to both directions of lateral distance from the drain and depth of the soil, to find out the distribution of ground improvement and strength enhancement. From the analysis on the distribution of water content, the gradual application of vacuum pressure to higher level by pre-determined stages starting from low vacuum pressure is found to be effective and desirable. In the comparison of the degrees of consolidation with elapsed time, the calculated value by the prediction method based on the Barren's conventional theory showed a good agreement with the measured value. With this, It is positively considered that the applicability of the prediction method based on Barren's theory to the practical design of horizontal drains can be justified such as in the calculation of drain spacing and consolidation period.

• Journal of Korean Society on Water Environment
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• v.34 no.4
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• pp.375-381
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• 2018

The monitoring of phytoplankton biomass and community structure is essential as a first step to control the harmful cyanobacterial blooms in freshwater systems, such as seen in rivers and lakes, due to the process of eutrophication and climate change. In order to quantify the biomass of phytoplankton with a wide range in size and shape, the measurement of cell biovolume along with cell density is required for a comprehensive review on this issue. However, most routine monitoring programs preserve the gathered phytoplankton samples before analysis using chemical additives, because of the constraint of time and the number of samples. The purpose of this study was to investigate the cell biovolume change characteristics of six cyanobacterial species, which are common bloom-causing cyanobacteria in the Nakdong River, after the preservation with Lugol's iodine solution. All species showed a statistically significant difference after the addition of Lugol's iodine solution compared to the live cell biovolume, and the cell biovolume decreased to the level of 34.0 ~ 56.3 % at maximum in each species after the preservation. The nonlinear regression models for determining the shrinkage ratio by a preservation period were derived by using the cell biovolume measured until 180 days preservation of each target species, and the equation to convert the cell biovolume measured after preservation for a certain period to the cell biovolume of viable cell was derived using that formula. The conversion equation derived from this study can be used to estimate the actual cell biovolume in the natural environment at the time of sampling, by using the measured biovolume after the preservation in the phytoplankton monitoring. Moreover this is expected to contribute to the final interpretation of the water quality and aquatic ecosystem impacts due to the cyanobacterial blooms.

• Journal of Korea Water Resources Association
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• v.54 no.6
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• pp.395-405
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• 2021

When issuing forecasts and alerts for agricultural drought, the relevant ministries only rely on the observation data from the reservoirs managed by the Korea Rural Community Corporation, which creates gaps between the drought analysis results at the local (si/gun) governments and the droughts actually experienced by local residents. Closing these gaps requires detailed local geoinformation on reservoirs, which in turn requires the information on reservoirs managed by local governments across Korea. However, installing water level and flow measurement equipment at all of the reservoirs would not be reasonable in terms of operation and cost effectiveness, and an alternate approach is required to efficiently generate information. In light of the above, this study validates and calibrates the parameters of the TANK model for reservoir basins, divided them into groups based on the characteristics of different basins, and applies the grouped parameters to unmeasured local government reservoirs to estimate and assess inflow. The findings show that the average determinant coefficient and the NSE of the group using rice paddies and inclinations are 0.63 and 0.62, respectively, indicating better results compared with the basin area and effective storage factors (determinant coefficient: 0.49, NSE: 0.47). The findings indicate the possibility of utilizing the information regarding unmeasured reservoirs managed by local governments.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.spc
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• pp.18-22
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• 2022

Because it is difficult to apply direct and optical detection techniques to sunken hazardous and noxious substances (HNS), effective acoustic detection techniques are required to detect sunken HNS in water. In this study, the possibility of acoustic detection of sunken HNS was investigated through backscattering signal measurement experiments using chloroform, a sunken HNS. After establishing a pool in an acrylic tank, backscattering signals were measured according to the presences or absence of chloroform by varying the grazing angle from 90° to 50° in 0.5° intervals using a pan&tilt system. A directional transducer transmitted and received sinusoidal signals with a frequency of 200 kHz and a pulse length of 25 ㎲ in a monostatic state. When chloroform was deposited, the received level of the backscattering signal at the interface between water and chloroform became low at a grazing angle of approximately 80° or smaller. Based on the backscattering signal results obtained at the interface between water and chloroform, the possibility of acoustic detection of sunken HNS was demonstrated.

• Korean journal of food and cookery science
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• v.2 no.2
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• pp.43-54
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• 1986

Back-pyun is a kind of rice cake made by steaming rice flour added sugar, water and salt and garnished with shredded chestnuts, dates, mushrooms and pinenuts. The Back-pyun has been widely used in Korean celebrations. This study aimed to compare and determine the effects on sensory characteristics and texture of Back-pyun 1) when the proportion of glutinous rice, added i.1 making Back-pyun, is 0%, 5%, 10%, 15%, 20%, 25% and 30% 2) then it reheated after storage for 0,24, 48 and 72 hours. Evaluation was conducted through sensory evaluation and objective evaluation. The results are as follows : 1. In sensory evaluation, color of Back-pyun turned yellow as the addition level of glutinous rice increased and then by reheating after storage for 24, 48 and 73 hours. Coarseness was not significantly different as the addition level of glutinous rice increased before storage. But Back-pyun reheated after storage for 24,48 and 72 hours were significantly different by the addition level of glutinous rice. Softness, dryness and crumblyness tended to decrease as the addition level of glutinous rice increased and by reheating after storage for 24, 48 and 72 hours. The most favorite tendency on the appearance was at the addition level of glutinous rice of 5%. Texture and overall preference were better at the addition level from 10% to 20% than any other levels. 2. Rheometer measurement indicated that compression force, gumminess ana chewiness tended to increase as the addition level of glutinous rice increased and decrease by the storage conditions. But work ratio and recovered height did not have much effect the addition level of glutinous rice and the storage conditions. Compression force of Back-pyun (not reheated) tended to increase by the storage time and the addition level of glutious rice. Especially it showed remakably increasing tendency during 24 hours storage (at $4^{\circ}C$) 3. Moisture content tended to increase by the addition level of glutinous rice and decrease by the storage time. But moisture content of Back-pyun reheated after storage for 24 hours stowed the increasing tendency. 4. Softness of Back-pyun had significant relationship with compression force and work ratio. Dryness and crumblyness had significant relationship with work ratio. Therefore compression force and work ratio represented the texture of Back-pyun.

• Analytical Science and Technology
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• v.28 no.6
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• pp.377-384
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• 2015

A survey of the artificial radionuclides in rivers and lakes was conducted to investigate their levels in surface water. Water samples were collected at 60 points and analyzed by gamma-ray spectrometry with a measurement time of 10,000 seconds for ¹³⁴Cs, ¹³⁷Cs, and ¹³¹I. The obained values were lower than MDA for all points, except one point for ¹³¹I that was 0.533±0.058 Bq/L. ¹³¹I is known as a radioactive material that occurs frequently in sewage treatment plants. Because it is often used for medical treatments and subject to spreading into the environment due to the excretion from the patients. For the point where ¹³¹I was detected, we conducted additional investigation on the upstream river point and the effluent points of nearby sewage treatment plant to find the source of ¹³¹I. ¹³¹I was not detected at the upstream points of one of the upstream sewage treatment plants but found at the downstream points with the level being 0.257±0.034 to 0.799±0.051 Bq/L, proving the sewage treatment plant was the ¹³¹Isource.

• Bulletin of the Korean Chemical Society
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• v.34 no.6
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• pp.1698-1702
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• 2013

Glucose is a common medical analyte measuring in human serum or blood samples. The development of a primary method is necessary for the establishment of traceability in measurements. We have developed an isotope dilution liquid chromatography tandem mass spectrometry as a primary method for the measurement of glucose in human serum. Glucose and glucose-$^{13}C_6$ in sample were ionized in ESI negative mode and monitored at mass transfers of m/z 179/89 and 185/92 in MRM, respectively. Glucose was separated on $NH_2P$-50 2D column, and the mobile phase was 20 mM $NH_4OAc$ in 30% acetonitrile/70% water. Verification of this method was performed by the comparison with NIST SRMs. Our results agreed well with the SRM values. We have developed two levels of glucose serum certified reference material using this method and distributed them to the clinical laboratories in Korea as samples for proficiency testings. The expended uncertainty was about 1.2% on 95% confidence level. In proficiency testings, the results obtained from the clinical laboratories showed about 3.6% and 3.9% RSD to the certified values. Primary method can provide the traceability to the field laboratories through proficiency testings or certified reference materials.

• International Journal of Fluid Machinery and Systems
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• v.9 no.2
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• pp.182-193
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• 2016

Introduction of intermittent electricity production systems like wind and solar power to electricity market together with the deregulation of electricity markets resulted in numerous start/stops, load variations and off-design operation of water turbines. Hydraulic turbines suffer from the varying loads exerted on their stationary and rotating parts during load variations since they are not designed for such operating conditions. Investigations on part load operation of single regulated turbines, i.e., Francis and propeller, proved the formation of a rotating vortex rope (RVR) in the draft tube. The RVR induces pressure pulsations in the axial and rotating directions called plunging and rotating modes, respectively. This results in oscillating forces with two different frequencies on the runner blades, bearings and other rotating parts of the turbine. This study investigates the effect of transient operations on the pressure fluctuations exerted on the runner and mechanism of the RVR formation/mitigation. Draft tube and runner blades of the Porjus U9 model, a Kaplan turbine, were equipped with pressure sensors for this purpose. The model was run in off-cam mode during different load variations. The results showed that the transients between the best efficiency point and the high load occurs in a smooth way. However, during transitions to the part load a RVR forms in the draft tube which induces high level of fluctuations with two frequencies on the runner; plunging and rotating mode. Formation of the RVR during the load rejections coincides with sudden pressure change on the runner while its mitigation occurs in a smooth way.