• 한국동물위생학회지
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• 제42권4호
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• pp.289-296
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• 2019

A novel rapid procedure with liquid chromatography tandem mass spectrometry (LC-MS/MS) detection has been developed by changing various conditions including sample preparation such as QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology. This work has been involved the optimization and validation of detection method for fluoroquinolones which are widespread used in livestock especially in the chicken. Five grams of homogenized chicken muscle were extracted with QuEChERS EN and acetonitrile containing 5% formic acid and cleaned with anhydrous magnesium sulfate and C₁₈ sorbent. The separation was performed on Acquity UPLC HSS T3 (2.1 mm×100 mm, 1.8 ㎛) column. The mobile phase A and B were composed of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid, respectively. Flow rate was 0.25 mL/min and column temperate was 40℃. LC-MS/MS with multiple reaction monitoring has been optimized for ten fluoroquinolones (ciprofloxacin, danofloxacin, difloxacin, enrofloxacin, marbofloxacin, norfloxacin, ofloxacin, orbifloxacin, pefloxacin and sarafloxacin). The method developed in this study has been presented good linearity with correlation coefficient (R²) of 0.9971~0.9998. LOD and LOQ values ranged from 0.09 to 0.76 ppb and from 0.26 to 2.29 ppb, respectively. The average recoveries were from 77.46 to 111.83% at spiked levels of 10.0 and 20.0 ㎍/kg. Relative standard deviation (%) ranged 1.28~11.90% on intra-day and 3.10~8.38 % on inter-day, respectively. This analysis method was applicable to the livestock residue laboratories and was expected to be satisfactory for the residue surveillance system.

• 대한본초학회지
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• 제26권1호
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• pp.41-46
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• 2011

Objectives : To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated. Methods : A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin $(PE)E_2$ were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats. Results : The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on $PGE_2$ production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.

• Journal of Biosystems Engineering
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• 제22권4호
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• pp.469-478
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• 1997

This study was conducted to develop an automatic nutrient-solution mixing system for small-scale sewers. The nutrient-solution mixing system consisted of a low-cost and precise metering device and data acquisition ＆ control system with a personal computer. and, the metering device was composed of three parts those were supply pumps, metering cylinders and venturi tube. The system controlled electric conductivity(EC) and pH of nutrient-solution based on the time-based feedback control method with the information about temperature, EC, and pH of the nutrient-solution. The performance of the nutrient-solution mixing system was evaluated through the control of EC and pH while compared with those of commercial system. Also an experimental cultivation of tomato was conducted to verify and to improve the developed system. Results of this study were as follows. 1. The correlation coefficient of meteing device between the flow rate and operating time was 0.9999, and the linear reuession equation computed was y=21.759x, where y is the discharge($g$) and x is the operating time(s). 2. Calculated errors for the developed metering device and two commercial pump were $\pm$0.3% $\pm$2.45% and $\pm$1.38 % FS error respectively. 3. An automatic nutrient-solution mixing system based on a low-cost and precise metering device was developed. 4. The full scale errors of the developed system in controlling EC and pH at 23$\pm$1$^{\circ}C$ were $\pm$0.05mS/cm and $\pm$0.2, respectively 5. When using the commercial system, the controlled values of EC and pH of the 500 $\ell$ of water were 1.29 mS/cm and 6.1 pH for the setting points of 1.4 mS/cm and 6.0 pH respectively at 23$pm1^{\circ}C$. 6. The developed nutrient-solution control system showed $\pm$0.05 ms/cm of deviation from the setting EC value over the experimental cultivation period. 7. The deviation from the average values of Ca and Mg mass content in the several nutrient-solution were 0.5% and 1.8% respectively.

• Fisheries and Aquatic Sciences
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• 제21권9호
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• pp.26.1-26.8
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• 2018

Inclusion effect of soybean meal (SBM) and fermented SBM (FSM) in extruded pellet for juvenile abalone (Haliotis discus) was compared in abalone farm. Dietary inclusion effect of the combined macroalgae (MA) (Undaria pinnatifida and Hizikia fusiforme) and a single Saccharina japonica on abalone was also compared. Three thousand six hundred juvenile abalone were purchased from a private hatchery and acclimated to the experimental conditions for 2 weeks. Six 5-ton flow-through raceway tanks were used, and abalone were randomly distributed into tanks (n = 600 per tank). Three experimental diets were prepared in duplicate. Fish meal, FSM, corn gluten meal, and shrimp meal and wheat flour and dextrin were used as the protein and carbohydrate sources, respectively, in the FSM diet. MA was also included in the FSM diet. FSM and MA in the FSM diet were substituted with SBM at the expense of wheat flour and S. japonica, referred to as the SBM and SJ diets. The experimental diets were pelletized by an extruded pelleter. Water stability of nutrients in the experimental diets was monitored at 12, 24, and 48 h after seawater immersion. The experimental diets were fed to abalone once a day to satiation with a little leftover for 120 days. The retained crude protein and lipid and ash content of the extruded pellets were changed over all period of time. Weight gain and specific growth rate (SGR) of abalone fed the SBM diet were greater than those of abalone fed the FSM and SJ diets. Weight gain and SGR of abalone fed the SJ diet were also greater than those of abalone fed the FSM diet. The longest shell length, widest shell width, highest shell height, and greatest soft body weight were obtained in abalone fed the SBM diet, followed by the SJ and FSM diets. Proximates of the soft body of abalone were not different among the experimental diets. In conclusion, SBM was a superior protein source to FSM in extruded pellet for growth performance of abalone. Dietary inclusion of a single S. japonica was superior to the combined inclusion of U. pinnatifida and H. fusiforme in the production of abalone.

• 생약학회지
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• 제43권4호
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• pp.279-285
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• 2012

The Mume Fructus (MF) has been used for relieves cough, arrests arrest chronic diarrhea, treat fluid depletion, and treat ascariasis in Korea. In this study, a high-performance liquid chromatography (HPLC) method was established for simultaneous determination of six main components of MF. Additionally, we were investigated the anti-inflammatory and anti-allergic effects of MF extract on lipopolysaccharide (LPS)-treated RAW264.7 cells and tumor necrosis factor (TNF)-${\alpha}$/interferon (IFN)-${\gamma}$-treated HaCaT cells. The analytical column for separation was used a Gemini $C_{18}$ column maintained at $40^{\circ}C$. The mobile phase consisted of 1.0% (v/v) acetic acid in water (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 280 nm and 320 nm. We evaluated the inhibitory effect of MF extract on the production of inflammatory markers, nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) in LPS-stimulated RAW264.7 cells and thymus- and activation-regulated chemokine (TARC/CCL17) in TNF-${\alpha}$/IFN-${\gamma}$-treated HaCaT cells, respectively. We confirmed the genes expression related with TARC, macrophage-derived chemokine (MDC/CCL22) and regulated on activation, normal T cell expressed and secreted (RANTES/CCL5) in HaCaT keratinocyte cells by MF extract. The contents of the five compounds in MF were 0.22-1.01 mg/g. Also, the MF extract show inhibition of about 78% and 75% on NO and $PGE_2$ production at the concentration 1000 mg/mL in RAW264.7 cells. MF extract suppressed the hTARC level and genes expression such as TARC, MDC, and RANTES on TNF-${\alpha}$/IFN-${\gamma}$-treated HaCaT cells.

• 수산해양교육연구
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• 제5권1호
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• pp.31-44
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• 1993

Through the early 1900's, the evolution of the surface condenser was closely tied to the development of steam engine and the turbine. As the chemical and petroleum industries evolved in the 1900's, the use of surface condensers in many different processes. Today, industry uses condensers in many shapes and sizes. The actual condensation process occurs on the outside surface of tubes. The nature of this surface geometry affects the condenser's heat transfer performance. The first condensers were built with plain tubes. As tube manufacturing techniques advanced, manufacturers started making tubes with integral fins. In the 1940's, fin densities were limited to about 600 to 700 fins per meter(fpm) because of manufacturing procedure. Today new manufacturing techniques allow production of tubes with fin densities ranging from 750 to 1600 fpm. The integral-fin tubes investigated in this paper are nominally 19 mm diameter. Eight tubes have been used with trapezodially shaped integral-fins having fin density from 748 to 1654 fpm and 10, 30 grooves. For comparison, tests are made using a plain tube having the same inside diameter and an outside diameter equal to that at the root of the fins for the finned tubes. Betty and Katz's theoretical modelis is used to predict the R-11 condensation coefficient on horizontal integral-fin tubes having 748, 1024 and 1299 fpm. Experiments are carried out using R-11 as working fluid. The refrigerant condensates at a saturation state of $30^{\circ}C$ on the outside tube surface cooled by coolant. The amount of noncondensable gases present in the test loop is reduced to a negligible value by repeated purging. For a given heat input to the boiler and given cooling water flow rate, all test data are taken at steady state. The observed heat transfer enhancement for the finned and grooved tubes significantly exceeded that to be expected on grounds of increased area. For the eight fin tubes and one plain tube tested, the best performance has been obtained with a tube having a fin density of 1299 fpm, and a fin bight of 1.2mm and 30 grooves.

• 한국습지학회지
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• 제13권2호
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• pp.231-242
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• 2011

도시화가 진행될수록 건물의 옥상 및 교통관련 시설물 또한 증가하여, 녹지공간은 감소하고 불투수면은 증가하게 된다. 불투수면이 증가함에 따라, 도시내 자연적 물순환 시스템 파괴, 비점오염물질 증가, 생태축 단절 등과 같은 다양한 환경수리학적 문제점이 발생되고 있다. 최근 들어 국민들의 생활 및 의식수준이 향상되면서, 도시의 경제적인 측면뿐만 아니라 심미적인 측면, 환경적인 측면을 동시에 고려한 지속가능한 도시 개발이 요구되고 있다. 또한 강우유출수 관리에 대한 관심과 인식이 높아지면서 Low Impact Development(LID) 관련 기술의 개발 및 적용에 관한 연구가 활발히 진행되고 있으나, 국내 환경에 적합한 가이드라인, 설계기준 등은 매우 부족한 실정이다. 본 연구는 도시 및 도로가에 적용하기 위한 소규모 인공습지 기술을 개발하기 위하여, Pilot-scale 실험을 실시하였으며, 분석 결과, TSS, $COD_{Cr}$, TN, TP, Total Pb에 대한 평균저감효율이 각각 95%, 82%, 35%, 91%, 57%로 나타났다. 이러한 결과는 소규모 인공습지가 향후 도시 내 효율적인 물순환 시스템을 구축하고, 수생태계 보전에 기여할 수 있을 것으로 판단된다.

• 한국습지학회지
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• 제20권2호
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• pp.181-189
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• 2018

본 연구는 탐진강 하구역 생물군집의 군집 동태 및 분포 조건을 통해 기수지역 생태계 관리의 기초정보를 제공하기 위해 각 분류군별 전문가들이 정밀 조사한 결과를 분석하였다. 식생을 포함한 총 11개 생물 분류군에 대한 생물종의 다양성, 풍부성, 상관관계 및 생태적 특성을 분석하였다. 분석 결과 식생은 하천의 물리적 환경과 염도 등 서식처 환경조건에 따라 7개 상관형에 18개 단위식생의 분포가 확인 되었다. 생물종 다양성은 멸종위기야생생물 9분류군을 포함해 총 1,125개 분류군이 확인되었다. 각 분류군별 종조성과 분포 특성은 넓은 구간에 걸쳐 형성되는 자연성 높은 기수역의 하구역 특성을 잘 반영하고 있었다. 특히, 하천을 중심으로 유속과 염도, 토성 등 기반환경 차이에 따른 종다양성과 그 분포가 뚜렷하게 구분되었다. 일부구간에 분포하는 물막이보, 인공제방과 같은 인위적 교란요소에도 불구하고 현재의 환경조건 하에서 생물종의 다양성과 그 분포는 전반적으로 자연성 높은 것으로 평가되었다. 그러나 물막이보, 간척, 주기적인 제방관리, 경작지 확장 등에 의한 야생생물의 서식처 소실과 축소, 생태계 연결성 단절 등은 지역 생태계의 다양성과 건강성을 위협하는 주요 요인으로 지목되었다. 탐진강 하구역의 생물 분포 현황과 그 특성을 규명하는 최초의 정밀조사로서 탐진강을 비롯한 하구역에 대한 각종 개발압력 및 훼손 위기에 대응 할 수 있는 기초자료로 활용될 수 있을 것으로 기대한다.

• 자원환경지질
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• 제29권3호
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• pp.315-323
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• 1996

인회석 배수로가 1994년 9월 30일 미국 인디아나주 중서부에 위치한 Green Valley 폐탄광에 건설되었다. 현장설치의 일차적인 목적은 현장조건에서 산성수에 대한 인회석 배수로의 장기적인 처리능력을 평가하는 데 있다. 이 배수로는 실내실험결과에 따라 설계되었는데 연장 9m, 폭 3.3m, 심도 0.75m이며，95mm~30번 채 크기의 인회석광 (francolite)으로 채워져 있고 복구된 폐석더미로 부터 흘러나오는 산성수가 유입되게 되어 있다. 본 배수로는 폭우 및 실트의 퇴적으로 인한 손상을 막기 위해 석회석 자갈과 filter fabric으로 덮여져 있다. 본 배수로는 플로리다주의 인광석광산에서 채광된 50톤의 인회석으로 채워져 있다. 인회석은 산성수내의 첼 최대 4,200 mg/l, 알루미늄 최대 830 mg/l, 황산염 최대 13,430 mg/l를 제거하였다. 산도는 거의 일정하였으며 3.1에서 4.3까지 변화를 보였다. 배수로 하류에서 측정한 유출량은 3~4.5 l/m였다. 철 및 알루미늄 인산염 침전물은 노란색 및 흰색 부유성 입자인데 침전못에 계속적으로 퇴적되었다.

• 한국환경복원기술학회지
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• 제15권2호
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• pp.19-29
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• 2012

Numerous studies suggested that fish assemblage structure reflects the status of stream ecosystems. The status of streams integrity, including various trophic levels, water quality and habitat degradation, can be assessed by fish assemblages. In this study, we investigated the relationships between fish assemblages and streamline geometry of streams. Previous studies suggested that geomorphologic parameter can be a critical factor of permeability between adjacent two systems. From a landscape ecological perspective, edges may partially control the flow rate of energy between two adjacent systems. Thus, the Streamline geometry can be a geomorphologic parameter that exhibits the integrity of stream. We selected the Nakdong river for study areas, which is one of major rivers and the longest (525 km) River in South Korea. We used the revised IBI representing overall ecological characteristics of Korean fish assemblages and eight sub-assessment criteria of IBI, collected from 82 sampling sites in the Nakdong River. For calculating the Streamline geometry, we measured fractal dimension index that generally used in biology, ecology and landscape ecology. We used the digital land-use/land-cover map and generated a 1-km buffer for each sampling site and refined the shape of the Streamlines. Pearson correlation analyses were performed between Streamline geometry and IBI and sub-assessment criteria of IBI. The results show that IBI and eight sub-assessments of fish are significantly correlated with geometry of Streamline. The fractal dimension of Streamline geometry were related with IBI (r = 0.48) and six sub-assessments of IBI, including total number of native fish and native species, the number of riffle benthic species, sensitive species, tolerant species and native insectivore. Especially, the number of tolerant species(r = -0.52) and native insectivore(r = 0.52) show strong correlation with geometry of Streamline. These results indicate that lower Streamline geometry can result in poor fish assemblages, while higher geometry of Streamline can enhance fish assemblages by potentially supplying insects and better habitat conditions. We expect the results of our study to be useful for stream restoration and management. However, we see the necessity of study investigating the mechanisms how Streamline geometry affect fish assemblages.