• Journal of Korean society of Dental Hygiene
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• v.21 no.5
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• pp.507-515
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• 2021

Objectives: The aim of this study was to evaluate the extent of the potential erosion of enamel induced by three different types of commercial fermented milk using the pH cycle model. Methods: Specimens were treated and soaked up in three types of fermented milk and in mineral water for 10 min, four times a day for 8 days, and all of the specimens were immersed in artificial saliva outside of treatment times. The microhardness of the surface was measured by a microhardness tester, and a scanning electron microscope (SEM) was used to identify the enamel surface morphology. Results: The differences in the surface microhardness (ΔVHN) of enamel were different among the groups (p<0.05). The four groups were in descending order of ΔVHN: the liquid type group, condensed-drink type group, condensed-stirred type group, and control group. The liquid type group had a higher ΔVHN than the other two fermented milk groups (p<0.05). Based on SEM observation, the most severe surface damage was due to the liquid type of fermented milk. Conclusions: Customers' careful discretion is advised when purchasing these types of fermented milk. This information is anticipated to be of much value in the prevention of dental erosion.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.41 no.4
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• pp.399-404
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• 2021

From 1 January 2020, the limit for Sulphur in fuel oil used on board ships operating outside designated emission control areas will be reduced to 0.5 %. This regulation by international maritime organization (IMO) is able to significantly reduce the amount of Sulphur oxides (SOx) discharging from ships and should have environmental advantages and health for all over the world. To meet the regulation, in these days, wet scrubber system is being actively developed. However, this process leads to make washing wastewater. In this study, we evaluated ion exchange resin system in accordance with scrubber wastewater discharge regulation by IMO. Theoretical wastewater used as feed solution of lab scale water treatment systems. The results revealed that nitrate ion was removed selectively in spite of high TDS wash wastewater solution depending on ion exchange resin property. Moreover, it was possible to improve efficiency of the system by optimizing operating conditions.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.6
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• pp.737-743
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• 2021

In this paper, the current status of marine industrial facilities, regulatory legal systems, and emission status of hazardous and noxious substances (HNS) in these facilities were analyzed, and the direction of improvement of the regulatory system was presented accordingly. As a result of the analysis, it is estimated that about 1,100 marine industrial facilities are subject to the Marine Environment Management Act of 2007. It is estimated that 190 kinds of hazardous substances are discharged from these marine industrial facilities and are highly likely to flow into the ocean, of which 20 are estimated to be discharged into the water system. However, due to the lack of relevant laws and regulation, it is difficult to clearly determine whether the discharged material corresponds to an exceptional discharged material, making it difficult to effectively enforce regulations in the field. For this reason, effective regulatory enforcement is difficult in the regulatory field. The marine environment management law should clearly stipulate the exceptional emission standards and types of substances, and clarify the selection system, risk assessment system, and emission information collection and monitoring system for related Hazardous and Noxious Substances.

• Journal of Life Science
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• v.31 no.10
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• pp.960-968
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• 2021

Since microalgae research started on late 18 century, they have been recognized as one of the most important bioresources used in bioindustry. Owing to the large efforts paid to industrial application of this microorganisms, their importance on food/feed and bioactive compounds has been further extending into the environmental research areas including alternative energy resources, mitigation of the carbon emission, and waste-water treatment. However, despite the importance on their industrial application, the fundamental research field related to the long-term preservation of microalgae culture has not received much attention. However, a less labor intensive and cost-efficient preservation technology enabling biologically active and stable microalgae-culture provides a key success factor in the biotechnological application. Therefore, this study investigated various cutting-edge microalgae cryopreservation technologies currently developed so far, mainly targeting Chlorophyta, which occupies the largest taxon in the classification system of microalgae. In addition, for the development of successful cryopreservation technique, the key factors such as temperature control effect and preservative effect during cryopreservation of microalgae culture were investigated. In addition, the problems with current preservation technology that is being used in Korean domestic biological resource banks and the international microalgal resource banks are described. According to our investigation, currently no standard method for long-term preservation of microalgae is available due to their various morphological and physiological characteristics. To overcome such issues, much more efforts on fundamental research area on the identification of specific biomarker used for microalgae taxonomical classification and further systemic approaches based on strain-specific cryopreservation methods needed.

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.503-524
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• 2021

A largemouth bass (Micropterus salmoides) is an ecosystem disturbance fish species at the highest rank in the aquatic ecosystem, causing a serious imbalance in freshwater ecosystems. Although various attempts have been made to eradicate and control largemouth bass, no effective measures were found. Therefore, it is necessary to find an approach to maximize the effective population reduction based on the unique characteristics of largemouth bass. This study used the transcriptome analysis to derive 182,887 unigene contigs and select 12 types of final target sequences for applying the CRISPR/Cas9 system in the genes of IZUMO1 and Zona pellucida sperm-binding protein, which are proteins involved in sperm-egg recognition. After synthesizing 12 types of sgRNA capable of recognizing each target sequence, 12 types of Cas9-sgRNA ribonucleoprotein (RNP) complexes to be used in subsequent studies were prepared. This study searched the protein-coding gene of sperm-egg through the Next Generation Sequencing (NGS) and edited genes through the CRISPR/Cas9 system to induce infertile individuals that produced reproductive cells but could not form fertilized eggs. Through such a series of processes, it successfully established a composition development process for largemouth bass. It is judged that this study contributed to securing the valuable basic data for follow-up studies to verify its effect for the management of ecological disturbances without affecting the habitat of other endemic species in the same water system with the largemouth bass.

• Journal of Animal Science and Technology
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• v.63 no.5
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• pp.1126-1141
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• 2021

Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42℃ for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 ㎍/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 ㎍/mL (MET70, 70 ㎍/mL of Met), 20 ㎍/mL (MET80, 80 ㎍/mL of Met), and 30 ㎍/mL (MET90,90 ㎍/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 ㎍/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 ㎍/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 ㎍/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.

• Korean Journal of Remote Sensing
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• v.37 no.6_1
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• pp.1709-1718
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• 2021

The waveform data of the Airborne Bathymetric LiDAR (ABL; LiDAR: Light Detection And Ranging) system provides data with improved accuracy, resolution, and reliability compared to the discrete-return data, and increases the user's control over data processing. Furthermore, we are able to extract additional information about the return signal. Waveform decomposition is a technique that separates each echo from the received waveform with a mixture of water surface and seabed reflections, waterbody backscattering, and various noises. In this study, a new waveform decomposition technique based on a Gaussian model was developed to improve the point extraction performance from the ABL waveform data. In the existing waveform decomposition techniques, the number of decomposed echoes and decomposition performance depend on the peak detection results because they use waveform peaks as initial values. However, in the study, we improved the approximation accuracy of the decomposition model by adding the estimated potential peak candidates to the initial peaks. As a result of an experiment using waveform data obtained from the East Coast from the Seahawk system, the precision of the decomposition model was improved by about 37% based on evaluating RMSE compared to the Gaussian decomposition method.

• Ocean and Polar Research
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• v.43 no.4
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• pp.307-320
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• 2021

Udo Rhodolith Beach is a small-scale, mixed sand-and-gravel beach embayed on the N-S trending rocky coast of Udo, Jeju Island, South Korea. This study analyzes the short-term topographic changes of the beach during the extreme storm conditions of four typhoons from 2016 to 2020: Chaba (2016), Soulik (2018), Lingling (2019), and Maysak (2020). The analysis uses the topographic data of terrestrial LiDAR scanning and drone photogrammetry, aided by weather and oceanographic datasets of wind, wave, current and tide. The analysis suggests two contrasting features of alongshore topographic change depending on the typhoon pathway, although the intensity and duration of the storm conditions differed in each case. During the Soulik and Lingling events, which moved northward following the western sea of the Jeju Island, the northern part of the beach accreted while the southern part eroded. In contrast, the Chaba and Maysak events passed over the eastern sea of Jeju Island. The central part of the beach was then significantly eroded while sediments accumulated mainly at the northern and southern ends of the beach. Based on the wave and current measurements in the nearshore zone and computer simulations of the wave field, it was inferred that the observed topographic change of the beach after the storm events is related to the directions of the wind-driven current and wave propagation in the nearshore zone. The dominant direction of water movement was southeastward and northeastward when the typhoon pathway lay to the east or west of Jeju Island, respectively. As these enhanced waves and currents approached obliquely to the N-S trending coastline, the beach sediments were reworked and transported southward or northward mainly by longshore currents, which likely acts as a major control mechanism regarding alongshore topographic change with respect to Udo Rhodolith Beach. In contrast to the topographic change, the subaerial volume of the beach overall increased after all storms except for Maysak. The volume increase was attributed to the enhanced transport of onshore sediment under the combined effect of storm-induced long periodic waves and a strong residual component of the near-bottom current. In the Maysak event, the raised sea level during the spring tide probably enhanced the backshore erosion by storm waves, eventually causing sediment loss to the inland area.

• Korean Journal of Organic Agriculture
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• v.28 no.4
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• pp.591-604
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• 2020

The objectives of this study were to find out the best condition of extracting methods of limonene from citron and to determine effects of limonene on immune modulation activity by measuring cytokine secretion using RAW 264.7 mouse macrophage cells. When distilled water was used as a solvent instead of organic solvents to extract limonene from citron, addition of refluxing process to simultaneous steam distillation extraction method was found to be much effective in extracting limonene. However, it required longer extraction time than using other organic solvents. Limonene extracts showed increased IL-β and IL-6 but decreased the TNF-α gene expression in limonene concentration dependant manner. However oral administration of limonene extracts to mice did not influence significantly compared to control in in vivo experiment. It might be due to that the mice were kept in well controlled and complete environment. Limonene, a natural material from citron has been approved to have a immune-modulation activity in the present study and have a potential as a feed additive that is environmentally friendly and no harmful. Further study with protected limonene, for example, for the protection of limonene from oxidation or bypass the ruminal degradation in order consequently to increase immune-modulation activity might be useful as a further research.

• Food Engineering Progress
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• v.14 no.3
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• pp.235-242
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• 2010

This study was to investigate the combined effect of enzyme and trehalose addition on the retrogradation rate and quality changes in a Korean traditional rice cake, Backsulgi. As for the enzyme, a commercial maltogenic amylase, Novamyl, was used. From texture profile analysis and sensory tests, the optimum enzyme content for the preparation of Backsulgi was determined to be 0.1%. Backsulgi was prepared with the fixed content of Novamyl (0.1%) and different ratios (5, 10, 15%) of trehalose, and physicochemical and sensory properties were examined for 3 days of storage at ${25^{\circ}C}$. As trehalose content increased, the water activity of Backsulgi decreased. In Hunter's color value, L- and a-values of trehalose-added samples were significantly lower than those of control. In texture profile analysis, hardness of Backsulgi decreased with trehalose content. During storage, a increase in hardness and a decrease in cohesiveness were typically observed for all samples. However, those changes were significantly reduced with trehalose content. The retardation of retrogradation with the enzyme and trehalose addition was confirmed using calculated Avrami rate and time constants. These results revealed that combined treatments of 0.1% Novamyl and 10% trehalose could produce Backsulgi of better sensory quality with retarded retrogradation compared to separated treatment.