• Korean Journal of Medicinal Crop Science
• /
• v.15 no.3
• /
• pp.162-169
• /
• 2007

Immune activities of two different extracts of Kadsura japonica Dunal. by typical extraction processes using water and ethanol at 60 $^{\circ}$C and 100 $^{\circ}$C were compared to them by ultrasofication system and through traditional fermemtation process. The fermented broth Kadsura japonica Dunal. definitely improved the growth of human B and T cell up to 30% and 22%, respectively, compared to the control. The secretion of TNF-${\alpha}$ and IL-60 was also enhanced by the addition of the fermented broth, up to 35%. NK cell activation was significantly improved up to 1.4 times higher than the case of adding other extracts. It was also found that this broth could yield higher nitric oxide production from macrophage than Lipopolysaccharides (LPS). It can be concluded that Kadsura japonica has immune activities and, in general, the culture broth from a conventional fermentation has higher immune activities, possibly by yielding immuno-modulatory compounds, not existed in typcial extraction systems as the result of HPLC analysis.

• Korean Journal of Medicinal Crop Science
• /
• v.10 no.3
• /
• pp.155-161
• /
• 2002

Using semi-selective (SS-) and selective (Jee-) medium, we identified the pathogens isolated from the symptomatic plants and soils collected from different locations, such as Suwon, Andong, and Youngju, as P. drechsleri, which is Phytophthora root rot causal agent of A. macrocephala. At $25^{\circ}C$, these isolates were grown faster on 10% V8A (V8 juice agar) medium than on PDA (potato dextrose agar) with hyphal swelling, but no growing was observed at below $5^{\circ}C$ and over $40^{\circ}C$. In order to identify the pathogenicity of each isolate, seedlings of A. macrocephala were inoculated with mycelium -zoospore suspended inoculum, which was prepared by culturing on 10% V8A medium and homogenizing in distilled water. By this method, wide ranges of pathogenicity were observed as follows; $5.0%{\sim}26.4%$ of disease severities concerning the lesion areas of the top plants and $23.5%{\sim}72.2%$ of disease incidences. Therefore, this was considered as a efficient method to identify the pathogenicity of P. drechsleri in large scale screening. P-A200073, isolated from soils in Andong, and P-9755, from the root of symptomatic plant of A. macrocephala in Suwon, showed the highest degree of pathogenicity to the seedlings. By these isolates, lesion areas and disease incidences of the inoculated seedlings were occurred $26.4%{\sim}63.2%$ and $25.1%{\sim}72.2%$, respectively. However, no symptoms were observed in uninoculated control. Same pathogens were reisolated from roots and lower stems of the inoculated plants, but not from leaves.

• The Korean Journal of Mycology
• /
• v.36 no.1
• /
• pp.84-92
• /
• 2008

Russula rosacea, one of edible and medicinal mushroom belonging Agaricales of Basidiomycota, has been known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at the concentration of $10{\sim}2000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of $21.4{\sim}45%$ in mice previously inoculated with Sarcoma 180. Fr. HW improved the immuno-potentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by 6.8 fold compared with control at the concentration of $500\;{\mu}g/ml$. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6 and 2.6 folds at the concentration of 50 mg/kg, respectively. Therefore, the antitumor effect against mice Sarcoma 180 by Russula rosacea could be due to immunomodulating activity.

• The Korean Journal of Mycology
• /
• v.36 no.1
• /
• pp.75-83
• /
• 2008

Lentinus giganteus, one of edible and medicinal mushroom belongs to Pleurotaceae of Agaricales, has been known to contain some inhibitive substances on Sarcoma 180 and curative effect on high blood pressure. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180 and HepG2 at the concentration of $10{\sim}2,000\;{\mu}g/ml$, but crude polysaccharides from Fr. NaCl was toxic to NIH3T3 at the concentration of $10{\sim}2,000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of $14.3{\sim}67.5%$ in mice previously inoculated with Sarcoma 180, respectively. Fr. NaCl exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by $1.53{\sim}1.68$ folds compared with control at the concentration of $50{\sim}200\;{\mu}g/ml$, respectively. The numbers of peritoneal exudate cells and circulating leukocytes were increased by 7.7 and 1.6 folds by injecting Fr. NaCl and Fr. MeOH into the mice at the concentration of 50 mg/ml body weight, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.13 no.1
• /
• pp.1-13
• /
• 1980

The purpose of the present study is to find the light intensity which induces maximum gathering rate and to observe the variation of the gathering rate both in daytime and at night by using three species of commercial fishes: filefish, Stephanolepis cirrhifer (Temminck et Schlegel), conger eel, Astroconger myriaster (Brevoort) and crucian carp, Carassius oarassius (Linnaeus). An experimental tank $(360L\times50W\times55Hcm)$ was set up in a dark room. An illumination system was attached to one end of the tank to control horizontal, light intensity. Six artificial light sources were prepared by combination of two light bulbs (5W, 150W) and seven filters. During the experiment water depth was maintained 50 cm level in the tank. The tank was marked into six longitudinal sections each being 60 cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 40 minutes prior to the main experiment. Upon turning on the light, the number of fish .in each section was counted 40 times every 30 seconds, and the gathering rates were obtain from the average number of fish in each section. In filefish the light intensity inducing maximum gathering rate was 0.7 lx(0.5-1.1 lx), and there nab little difference between day and night. Gathering rate varied propotionally to the length of illumination time. In conger eel the light intensities inducing maximum gathering rate were 1.9 lx(1.2-2.9 lx) in daytime and 5.2 lx (3.2-7.7 lx) at night. Thus, there was a considerable difference between day and night, but the gathering rate remained almost unchanged regardless to the length of illumination time. In crucian carp gathering rate did not show a definite pattern hut fluctuated irregularly. The gathering rate, however, was slightly higher at 16.21 lx, and there was little difference between day and night.

• Journal of Korean Neurosurgical Society
• /
• v.52 no.4
• /
• pp.281-287
• /
• 2012

Objective : To evaluate the effect of calcium supplementation on spinal bone fusion in ovariectomized (OVX) rats. Methods : Sixteen female Sprague Dawley rats underwent bilateral ovariectomy at 12 weeks of age to induce osteoporosis and were randomly assigned to two groups : control group (n=8) and calcium-supplemented group (OVX-Ca, n=8). Autologous spinal bone fusion surgery was performed on both groups 8 weeks later. After fusion surgery, the OVX-Ca group was supplemented with calcium in drinking water for 8 weeks. Blood was obtained 4 and 8 weeks after fusion surgery. Eight weeks after fusion surgery, the rats were euthanized and the L4-5 spine removed. Bone fusion status and fusion volume were evaluated by manual palpation and three-dimensional computed tomography. Results : The mean fusion volume in the L4-5 spine was significantly greater in the OVX-Ca group ($71.80{\pm}8.06mm^3$) than in controls ($35.34{\pm}8.24mm^3$) (p<0.01). The level of osteocalcin, a bone formation marker, was higher in OVX-Ca rats than in controls 4 weeks ($610.08{\pm}10.41$ vs. $551.61{\pm}12.34$ ng/mL) and 8 weeks ($552.05{\pm}19.67$ vs. $502.98{\pm}22.76$ ng/mL) after fusion surgery (p<0.05). The level of C-terminal telopeptide fragment of type I collagen, a bone resorption marker, was significantly lower in OVX-Ca rats than in controls 4 weeks ($77.07{\pm}12.57$ vs. $101.75{\pm}7.20$ ng/mL) and 8 weeks ($69.58{\pm}2.45$ vs. $77.15{\pm}4.10$ ng/mL) after fusion surgery (p<0.05). A mechanical strength test showed that the L4-5 vertebrae in the OVX-Ca group withstood a 50% higher maximal load compared with the controls (p<0.01). Conclusion : Dietary calcium given to OVX rats after lumbar fusion surgery improved fusion volume and mechanical strength in an ovariectomized rat model.

• the MEAT Journal
• /
• s.34 winter
• /
• pp.26-39
• /
• 2007

This study investigated the effect of salt and glucono-$\delta$-lactone (GdL) on the cold-set binding of restructured pork washed and pressurized at 200 MPa. Binding strength, pH, water holding capacity (WHC) and color were determined. NaCl improved pH, WHC and binding strength. GdL also increased binding strength while decreased WHC and pH significantly (p<0.05). However, low GdL level combined with NaCl showed high pH and WHC, compared to control. In color, NaCl decreased L*-value with increasing a*-value significantly (p<0.05). In contrast to NaCl, GdL increased L*-value and decreased a*-value. GdL tended to decrease b*-value and significant differences were found when GdL was added above 1%. Pearson’s correlation coefficients presented that NaCl had a significant effect on binding strength (0.6632) and lightness (?0.7330) while GdL had a significant correlation with all parameters barring binding strength. The results indicated that under washing and pressure treatments, GdL had a potential effect on cold-set binding with reducing NaCl concentration, especially when low GdL concentration combined with NaCl was added.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.46 no.3
• /
• pp.117-129
• /
• 2018

With the changing of social needs and paradigms, citizens are concerned about aesthetic and cultural community gardens from productive urban farms in Korea. It is still difficult, however, to cultivate a beautiful garden with a community to design, install and manage it. Therefore, this study analyzed the community garden design guidelines of other countries to derive the characteristics and implications. The research method analyzed six guidelines for community garden design in the UK, Canada, the USA, Australia and Japan. As a result of the study, most community garden design guidelines are opened freely via on-line service. The guidelines are composed with chronological processes such as intro, site selection, design, construction and maintenance. The introduction section treats definition, purpose, meaning, efficiency and success factors for community gardens. Site selection emphasizes site conditions (soil, light, shadow, water, etc.), landholding and insurance. The design section, however, lacks adequate drawings and case images. These guidelines offer little explanation with few illustrations and sentences. Construction sections explain about plantings, facilities, expense and details. The maintenance guidelines of community gardens contain how to control members, funding, harvesting, pests and plants. In addition, some guidelines include vandalism, organic cultivation methods, recording and advertisement. Lastly, the appendix contains a variety of checklists, administrative documents, guidelines, drawings, and contacts. This study will help understand the trends and characteristics of overseas community gardens design guidelines so as to also supply directions for guidelines to be introduced in Korea.

• Atmosphere
• /
• v.28 no.1
• /
• pp.99-112
• /
• 2018

In this study, the accuracy of ocean analysis data, which are produced from the Korea Meteorological Administration (KMA) Nucleus for European Modelling of the Ocean/Variational Data Assimilation (NEMO/NEMOVAR, hereafter NEMO) system and the HYbrid Coordinate Ocean Model/Navy Coupled Ocean Data Assimilation (HYCOM/NCODA, hereafter HYCOM) system, was evaluated using various oceanic observation data from March 2015 to February 2016. The evaluation was made for oceanic thermal environments in the tropical Pacific, the western North Pacific, and the Korean peninsula. NEMO generally outperformed HYCOM in the three regions. Particularly, in the tropical Pacific, the RMSEs (Root Mean Square Errors) of NEMO for both the sea surface temperature and vertical water temperature profile were about 50% smaller than those of HYCOM. In the western North Pacific, in which the observational data were not used for data assimilation, the RMSE of NEMO profiles up to 1000 m ($0.49^{\circ}C$) was much lower than that of HYCOM ($0.73^{\circ}C$). Around the Korean peninsula, the difference in RMSE between the two models was small (NEMO, $0.61^{\circ}C$; HYCOM, $0.72^{\circ}C$), in which their errors show relatively big in the winter and small in the summer. The differences reported here in the accuracy between NEMO and HYCOM for the thermal environments may be attributed to horizontal and vertical resolutions of the models, vertical coordinate and mixing scheme, data quality control system, data used for data assimilation, and atmosphere forcing. The present results can be used as a basic data to evaluate the accuracy of NEMO, before it becomes the operational model of the KMA providing real-time ocean analysis and prediction data.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.23 no.3
• /
• pp.33-41
• /
• 2015

An alternative was investigated to maximize the treatment efficiency of wastewater treatment plant without large scale expansion. 79% of detention time was required for enough nitrogen control. As aeration time was extended, nitrification was processed, but opposite trend was seen in phosphorus. High concentration of $NO_3-N$ interrupted PAOs activity not to absorb phosphorus. An alternative was devised for selective use of anaerobic or anoxic zone. Trisection was suggested for alternative use of center room. The result was relatively successful. The concentration of phosphorus was reduced with reduction of nitrogen. Extended anaerobic condition seemed to stimulate denitrification. Valve connection of internal return from aeration tank will make it possible to use middle room alternatively. This method will be a good alternative for seasonal variation of water temperature.