• Korean Journal of Pharmacognosy
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• v.43 no.1
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• pp.10-15
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• 2012

An accurate and sensitive analysis method was established for simultaneous determination of three bioactive compounds (glycyrrhizin, 6-gingerol and ginsenoside Rg3) in the Ejung Tang with high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-Mass spectrometry (MS). The optimizing chromatographic separations a were acquired by an $C_{18}$ column ($5{\mu}m$, $4.6I.D{\times}250mm$, SHISHEDO) using gradient elution with water comprising 0.1% TFA(trifluoroacetic acid) and acetonitrile at a performing temperature of $35^{\circ}C$. Flow rate was 1.0 ml/min. A detection UV wavelength set at 205 nm and 250 nm. The three compounds were identified by electrospray ionization mass spectrometry. All calibration curves indicated great linear regression within test ranges ($R^2>0.9997$). The established method provided acceptable precision and accuracy. The relative standard deviations (RSDs) of intra-day and inter-day were less than 2.00% and 3.00%, respectively. The recoveries were found to range from 94.49 to 101.10% for the three compounds analyzed. These results showed that this method was effective and reliable for quality control of Eiung-Tang.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.414-420
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• 2015

Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose disease development, which showed no inhibitory effects by tea saponin. NSC extractum controlled in vitro C. musae growth through directly inhibiting germination rate and germ tube elongation, and causing distortation, rupture and indentation of C. musae mycelium. In banana fruit subject to C. musae inoculation, higher PAL, POD, GLU and CHT activity was observed in banana fruit treated with crude NSC extractum than that of water control fruits. Current study proved the best controlling effects of crude NSC extractum in C. musae in vitro and in vivo development, which through direct inhibition of C. musae growth and increasing defense system of the banana fruit.

• Journal of the Korean Wood Science and Technology
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• v.40 no.1
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• pp.53-59
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• 2012

This study used pine wilt disease damaged trees as raw materials. The damaged woods were subjected to soaking treatment using wood vinegar, nematicide and sea water, fumigated treatment using fumigation, and the bending strength and hardness of the timber were measured at different points of time after the treatment. In soaking treatment, the highest bending strength was obtained from wood vinegar (100 fold dilution)-soaked specimens of Pinus densiflora and the nematicide-soaked specimens of Pinus thunbergii. The highest hardness in cross section was gained from the nematicide-soaked specimens of Pinus densiflora and Pinus thunbergii, the highest hardness in radial section was obtained from the nematicide-soaked specimens of Pinus densiflora and wood vinegar (100 fold dilution)-soaked specimens of Pinus thunbergii, and the highest hardness in tangential section was obtained from the nematicide-soaked specimens of Pinus densiflora and Pinus thunbergii, and there were not significantly different among the treatments. When fumigated treatment was applied, bending strength, hardness was somewhat lower in the control but were not significantly different between the treatments.

• Journal of Chest Surgery
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• v.18 no.4
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• pp.822-834
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• 1985

Reexpansion of the lung is the most desirable method of filling the pleural space whether it`s contents may be, and closed thoracotomy connected to a water-seal drainage remains the basic therapeutic modality in the treatment of the problems of the pleural space. We usually used rubber mushroom tubes, size No. from 16 to 34 Fr., and performed closed thoracotomy after preliminary thoracentesis to determine the exact depending position. Author reviewed 576 cases of closed thoracotomy which were performed in the Department of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, since Jan., 1980 to June, 1984. The results were as follows: 1. The age distribution was 10 days to 76 years old and mean age of the patients was 36.6 year, and the most prevalent age group was twenties, and sex predisposition was male dominant, 86.9%. 2. The most common etiologic disease group was pneumothorax and the most common etiologic disease was traumatic hemothorax. 3. Sites of tubing were predominantly at posterior axillary line, 7th intercostal space and midclavicular line, 2nd intercostal space even though frequent presence of free pleural space. The two sides, right and left difference of occurrence rate was more frequent at right side, 51.2%. 4. Usually the durations of tubing was less than 10 days, 52.6%, and the number of tubes used to the same patient concomitantly was one, 73.9%, and the time of tubing to the same patient was 1st, 83.6%. 5. The common symptom and sign were dyspnea, 50.0%, chest pain, 30.7%, cough, 10.7%, fever, 6.5%. Especially, fever and cough was the most common symptom and sign in pyogenic empyema, 59.3%. 6. The common etiologic lesions of pneumothorax were blebs and bullae, 73.3%, and of pyogenic empyema was pneumonia, 69.0%. 7. The complication rate of closed thoracotomy was 26.0%. Among these complications, infection was 44.7%, and intercostal neuralgia was 25.3%. 8. 70.9% of all patients recovered with only closed thoracotomy and the rest of patient needed additional some necessary managements such as open thoracotomy [Blebectomy, Resection, Pleurodesis, Decortication, Bleeding control], open drainage, thoracoplasty and so on to have successful results.

• Membrane Journal
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• v.25 no.5
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• pp.456-463
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• 2015

In this study, we synthesize novel silane based inorganics for preparation of the polymer electrolyte membrane with high proton conductivity under high temperature condition and developed membranes are characterized. SPAES, hydrocarbon based polymer are synthesized and used as main polymeric material. We used sol-gel method to prepare inorganic material with high performance using silica, phosphate and zirconium. Three types of inorganics were prepared by control of the mole ration of each component. As a result of EDX analysis, the inorganic materials are well dispersed in the polymer membrane. The water uptake of the composite membrane is increased by introduction of the hydrophilic inorganic material in the membrane. When the content of the zirconium in the membrane is increased, the proton conductivity of the composite membrane shows the higher value than pure SPAES membrane at the high temperature. And the silica based inorganics effect to increase the proton conductivity under low temperature condition.

• Korean Journal of Soil Science and Fertilizer
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• v.10 no.2
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• pp.85-92
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• 1977

The six soybean cultivars (Lee, Hill, Harosoy, Clark-63 Chippewa and R56-49) different in phosphorus sensitivity were cultured with $NH_4-N$, $NO_3-N$ or urea-N under water culture condition. Free sugars and organic chrematogram. Three peaks (unknown x, y and sucrose) were appeared as considerable main peaks. The X compound appeared as trace in the nitrate fed plant while unexpectedly high in ammonium or urea fed plant. The Y compound tend to decrase in urea fed plant. Sucrose was trace in ammonium fed plant but it was greater in urea onethan in nitrate one. The X was assumed a four carbon sugar acid derived from erythrose or a ring compound derived from purine or pyrimidine. While Y was assumed a hexose derived from glycolysis path. Since Y/x ratio is a good index of phosphorus sensitivity (inve rserelation) these compounds appears keycompounds to elucidate phosphorus sensitivity and ammonium toxicity.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.270.1-270.1
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• 2013

Zinc oxide (ZnO) is one of the most powerful materials for purifying organic pollutants using photocatalytic activity. In this study, we have introduced a novel method to design highly photoreactive flexible 3 dimensional (3D) ZnO nanocomposite [F-ZnO-m (m: reaction time, min)] by electrospinning and simple-step ZnO growth processing (one-step ZnO seed coating/growth processing). Significantly, the F-ZnO-m could be a new platform (or candidate) as a photocatalytic technology for both morphology control and largearea production. The highest photocatalytic degradation rate ([k]) was observed for F-ZnO-m at 2.552 h-1, which was 8.1 times higher than that of ZnO nanoparticles (NPs; [k] = 0.316 h-1). The enhanced photocatalytic activity of F-ZnO-m may be attributed to factors such as large surface area. The F-ZnO-m is highly recyclable and retained 98.6% of the initial decolorization rate after fifteen cycles. Interestingly, the F-ZnO-m samples show very strong antibacterial properties against both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) after exposure to UV-light for 30 min. The antibacterial properties of F-ZnO-m samples are more effective than those of ZnO NPs. More than 96.6% of the E. coli is sterilized after ten cycles. These results indicate that F-ZnO-m samples might have utility in several promising applications such as highly efficient water/air treatment and inactivation of pathogenic microorganisms.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.281.2-281.2
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• 2013

Since heavy metal ions included in water or food resources have critical effects on human health, highly sensitive, rapid and selective analysis for heavy metal detection has been extensively explored by means of electrochemical, optical and colorimetric methods. For example, quantum dots (QDs), such as semiconductor QDs, have received enormous attention due to extraordinary optical properties including high fluorescence intensity and its narrow emission peaks, and have been utilized for heavy metal ion detection. However, the semiconductor QDs have a drawback of serious toxicity derived from cadmium, lead and other lethal elements, thereby limiting its application in the environmental screening system. On the other hand, Graphene oxide (GO) has proven its superlative properties of biocompatibility, unique photoluminescence (PL), good quenching efficiency and facile surface modification. Recently, the size of GO was controlled to a few nanometers, enhancing its optical properties to be applied for biological or chemical sensors. Interestingly, the presence of various oxygenous functional groups of GO contributes to opening the band gap of graphene, resulting in a unique PL emission pattern, and the control of the sp2 domain in the sp3 matrix of GO can tune the PL intensity as well as the PL emission wavelength. Herein, we reported a photoluminescent GO array on which heavy metal ion-specific DNA aptamers were immobilized, and sensitive and multiplex heavy metal ion detection was performed utilizing fluorescence resonance energy transfer (FRET) between the photoluminescent monolayered GO and the captured metal ion.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.77.1-77.1
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• 2013

Atomic layer deposition (ALD) is known for its self-limiting reaction, which offers atomic-level controllability of the growth of thin films for a wide range of applications. The self-limiting mechanism leads to very useful properties, such as excellent uniformity over a large area and superior conformality on complex structures. These unique features of ALD provide promising opportunities for future electronics. Although the ALD of Al2O3 film (using trimethyl-aluminum and water as a metal precursor and oxygen source, respectively) can be regarded as a representative example of an ideal ALD based on the completely self-limiting reaction, there are many cases deviating from the ideal ALD reaction in recently developed ALD processes. The nonconventional aspects of the ALD reactions may strongly influence the various properties of the functional materials grown by ALD, and the lack of comprehension of these aspects has made ALD difficult to control. In this respect, several dominant factors that complicate ALD reactions, including the types of metal precursors, non-metal precursors (oxygen sources or reducing agents), and substrates, will be discussed in this presentation. Several functional materials for future electronics, such as higher-k dielectrics (TiO2, SrTiO3) for DRAM application, and resistive switching materials (NiO) for RRAM application, will be addressed in this talk. Unwanted supply of oxygen atoms from the substrate or other component oxide to the incoming precursors during the precursor pulse step, and outward diffusion of substrate atoms to the growing film surface even during the steady-state growth influenced the growth, crystal structure, and properties of the various films.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.489-496
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• 1995

Artemisia messes-schmidiana var viridis(Compositae) has been used for jaundice, hepatitis, diuretic and liver cirrhosis etc. 1-naphthylisothiocyanate(ANIT) has been used as a model compound to study mechanisms of intrahepatic cholestasis in laboratory animals as rat and mouse. The purposes of present study are to examine pharmacological effects of Artemisia messes-schmidiana var viridis water extract(AMWE) on alterations of triacylglycerol, cholesterol, protein, albumin and A/G ratio levels in serum, of histopathological appearances of liver, and that of hepatic microsomal cytochrome P-450 contents. Increased serum triacylglycerol levels by ANIT were significantly decreased with AMWE. However, AMWE posttreatment aggravated ANIT-induced cholesterol increase. Serum total protein and albumin contents, and A/G ratio were decreased in all ANIT-treated groups, and there were increased compared with control by AMWE posttreatment. Hepatic microsomal cytochrome P-450 contents were decreased in either AMWE and ANIT treatment, which greatly increased with AMWE pretreatment. On the other hand, in histological findings, our results shown that ANIT induced increase of lipid droplets and widening of sinusoidal capillary and these phenomena were disappeared with AMWE treatment. In conclusion, AMWE have choleresis effect. Also, AMWE improved lipid metabolism, protection and regeneration of hepatocytes in ANIT-induced cholestasis.