Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Simultaneous Determination of Three Compounds in Ejung Tang by HPLC-DAD and LC-ESI-MS

HPLC를 이용한 이중탕 중 3종 활성성분의 동시분석법 확립

  • Lee, Bo-Hyoung (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Jin-Yeul (TKM Converging Research Division, Korea Institute of Oriental Medicine) ;
  • Weon, Jin-Bae (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Yang, Hye-Jin (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Yun, Bo-Ra (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Choong-Je (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
  • 이보형 (강원대학교 생물소재공학전공) ;
  • 마진열 (한국한의학연구원) ;
  • 원진배 (강원대학교 생물소재공학전공) ;
  • 양혜진 (강원대학교 생물소재공학전공) ;
  • 윤보라 (강원대학교 생물소재공학전공) ;
  • 마충제 (강원대학교 생물소재공학전공)
  • Received : 2011.12.23
  • Accepted : 2012.02.27
  • Published : 2012.03.31
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Abstract

An accurate and sensitive analysis method was established for simultaneous determination of three bioactive compounds (glycyrrhizin, 6-gingerol and ginsenoside Rg3) in the Ejung Tang with high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray ionization (ESI)-Mass spectrometry (MS). The optimizing chromatographic separations a were acquired by an $C_{18}$ column ($5{\mu}m$, $4.6I.D{\times}250mm$, SHISHEDO) using gradient elution with water comprising 0.1% TFA(trifluoroacetic acid) and acetonitrile at a performing temperature of $35^{\circ}C$. Flow rate was 1.0 ml/min. A detection UV wavelength set at 205 nm and 250 nm. The three compounds were identified by electrospray ionization mass spectrometry. All calibration curves indicated great linear regression within test ranges ($R^2>0.9997$). The established method provided acceptable precision and accuracy. The relative standard deviations (RSDs) of intra-day and inter-day were less than 2.00% and 3.00%, respectively. The recoveries were found to range from 94.49 to 101.10% for the three compounds analyzed. These results showed that this method was effective and reliable for quality control of Eiung-Tang.

Keywords

References

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