• Title/Summary/Keyword: washing system

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Development of microarrayer for manufacturing DNA chip used in genome project (유전자 검색을 위한 DNA 칩 제작용 microarrayer의 개발)

  • Lee, Hyun-Dong;Kim, Ki-Dae;Kim, Chan-Soo;Lim, Yong-Pyo;Park, Jung-Kyu
    • Korean Journal of Agricultural Science
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    • v.30 no.1
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    • pp.76-88
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    • 2003
  • This study exploits the robot system which is necessary in gene study, bio-technology industry. As well, it can achieve the job of DNA chip manufacturing whose use rate has been increased recently. The robot consists of DNA spotting device for spotting DNA on the silylated slide and well plate, bed for fixing well-plate, washing & drying device of washing and drying the pin part of DNA spotting device, distillation-water vessel, and discharge vessel of wash water. We made the term of sticking DNA to the pin on well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin for 1 second. At this rate, if DNA is spotted in the minimum space possible of about 0.32mm, it can stick about 8,100 DNA spots on the well plate. Analyzing the procedure: Movement starts. Pin washes, dries, and smears DNA on the well plate. Spots DNA onto 12 chips takes 2 minutes and 50 seconds.

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Estimation of Potential Supply of Offset from Household Electric Appliances (가정용 전자기기의 잠재 상쇄 공급량 추정)

  • Jin, Hyun Joung;Kim, Jeong In;You, Eun Young;Park, Seo Hwa
    • Environmental and Resource Economics Review
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    • v.24 no.3
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    • pp.463-488
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    • 2015
  • A more detailed design of offset system is needed according to the emission trading system started in 2015. This study aims to estimate the supply of potential offset that can be secured by expanding high-efficiency household electric appliances. The target commodities for analysis are three different householding electric appliances: TV, washing machine, electric fan, refrigerator and air conditioner. By using the ARDL model, we estimated the coefficients of diffusion of these high-efficiency appliances from 2016 to 2022. Then, the potential supply of offset was drawn by calculating the amount of electricity saving by efficiency improvement and by applying the rates of carbon exchange. Supposing that the electricity savings rates of high-efficiency appliances are each 10% and 20%, the accumulated carbon decrement in 2022 was respectively $361,899CO_2t$ and $723,797CO_2t$. The appliance that showed the biggest carbon decrement was air conditioner, and the second biggest was refrigerator and the next was TV, followed by washing machine, electric fan.

Characteristics of Environmental Contamination for the Groundwater and Stream Water in the Vicinity of the Woonjeongdong Sanitary Landfill Area in Kwangju-city, Korea (광주광역시 운정동 위생매립장 주변 지하수와 하천수의 환경지구화학적 오염특성)

  • 오근창;윤정환;김경웅;박천영;김정빈
    • Economic and Environmental Geology
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    • v.34 no.6
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    • pp.523-537
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    • 2001
  • The physical properties of pH, turbidity, TDS and salt contents in the stream water near the entrance of Woonjcongdong sanitary landfill area with a drainage pipe are higher than those in neighbored drainage system, but DO it lower than that in neighbored drainage system blue to the eutrophication. In the ground water, pH, turbidity, TDS and salt contents at A ,F and C where may be under effect of the Woonjeongdong sanitary landfill area, are also shown higher values in contrast to other groundwater site, like B , D , and E , Particularly, the groundwater in A , F and C are plotted in Na-Cl type (If the piper diagram, which is an another important prolf of the contaminapion by the leachate and washing water from the Woonjeongdong sanitary landfill area. Some elements and physical properties of the waters in rainy season are desplayed a clear characteristics of contaminaton, compared to the theose of winter in thes study. Elements such as Ca, Mg, Na, K, Cl­,$SO_4^{2-} , \;and \;F­^-$) are accompanied well with each other by the result of water in the vicinity of landfillarea.

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Shape Optimization of Three-Way Reversing Valve for Cavitation Reduction (3 방향 절환밸브의 공동현상 저감을 위한 형상최적화)

  • Lee, Myeong Gon;Lim, Cha Suk;Han, Seung Ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.11
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    • pp.1123-1129
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    • 2015
  • A pair of two-way valves typically is used in automotive washing machines, where the water flow direction is frequently reversed and highly pressurized clean water is sprayed to remove the oil and dirt remaining on machined engine and transmission blocks. Although this valve system has been widely used because of its competitive price, its application is sometimes restricted by surging effects, such as pressure ripples occurring in rapid changes in water flow caused by inaccurate valve control. As an alternative, one three-way reversing valve can replace the valve system because it provides rapid and accurate changes to the water flow direction without any precise control device. However, a cavitation effect occurs because of the complicated bottom plug shape of the valve. In this study, the cavitation index and percent of cavitation (POC) were introduced to numerically evaluate fluid flows via computational fluid dynamics (CFD) analysis. To reduce the cavitation effect generated by the bottom plug, the optimal shape design was carried out through a parametric study, in which a simple computer-aided engineering (CAE) model was applied to avoid time-consuming CFD analysis and difficulties in achieving convergence. The optimal shape design process using full factorial design of experiments (DOEs) and an artificial neural network meta-model yielded the optimal waist and tail length of the bottom plug with a POC value of less than 30%, which meets the requirement of no cavitation occurrence. The optimal waist length, tail length and POC value were found to 6.42 mm, 6.96 mm and 27%, respectively.

Microbiological Evaluation for HACCP System Application of Green Vegetable Juice Containing Lactic Acid Bacteria (유산균을 함유한 녹즙의 HACCP 시스템 적용을 위한 미생물학적 위해도 평가)

  • Kwon, Sang-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.11
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    • pp.4924-4931
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    • 2011
  • This research performed to evaluate a production processes reporting by the HACCP system of green vegetable juice products, containing lactic acid bacteria, stage of processing raw materials agricultural products and production facilities of general bacteria and pathogenic micro organism. General bacteria are found from four samples of storage of agricultural products at process stage and water was detected 8.67~14.67 CFU/ml. However, all samples were detected less than 105 CFU/ml as a legal standards after the process of UV sterilization. For the outcome of experiment of E.coli, E.coli O157:H7, B.cereus, L.moonocytogenes, Salmonella spp, Staph.aureus as the food poisoning bacterial, E.coli was detected until UV pre-step process in storage process and B.cereus was detected partly till 1st washing. Since all bacterial, Yeast and Mold are detected in main materials, pre-control method is a necessary to establish for decreasing with a number of initial bacteria of main materials and it is considered to establish the effective ways of washing and sterilization such as production facilities for cross contamination prevention of bacteria and Sthaphylococcus. Based on above results, the process of UV sterilization should be managed with CCP as an important process to reduce or eliminate the general and food poisoning bacterial of green vegetable juice products, including lactic acid bacteria. Therefore, it is considered to need an exhaustive HACCP plan such as control manual of UV sterilization, solution method, verification, education and training and record management.

Implementation on the Portable Blood Gas Analyzer and Performance Estimation (휴대형 혈액가스분석 시스템의 구현 및 성능평가)

  • Jeong, Do-Un;Jeon, Gye-Rok;Bae, Jin-Woo;Kim, Gil-Jung;Sim, Yoon-Bo
    • Journal of Sensor Science and Technology
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    • v.12 no.1
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    • pp.34-43
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    • 2003
  • In this study, we implemented the measurement of pH, $pO_2$, $pCO_2$ of the arterial blood on a portable blood gas analysis system. This system is consist of two parts of hardware and software. The hardware part is divided into a fluidic mechanism and an electronic circuit unit. The system program is composed of operating, washing, correcting, and measuring routines. Both of 1-point and 2-point calibration schemes were used to enhance the accuracy of the measurement. In order to evaluate the performance of the developed system, we measured and performed statistical analysis on the characteristics of the sensing electrode response. As a result, coefficient variation was within 1.12, and maximum error was within 1.298%. We confirmed development possibility of portable blood gas analyzer.

A novel retentive type of dental implant prosthesis: marginal fitness of the cementless double crown type implant prosthesis evaluated by bacterial penetration and viability

  • Hong, Seoung-Jin;Kwon, Kung-Rock;Jang, Eun-Young;Moon, Ji-Hoi
    • The Journal of Advanced Prosthodontics
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    • v.12 no.4
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    • pp.233-238
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    • 2020
  • PURPOSE. This study aims to compare the marginal fitness of two types of implant-supported fixed dental prosthesis, i.e., cementless fixation (CL.F) system and cement-retained type. MATERIALS AND METHODS. In each group, ten specimens were assessed. Each specimen comprised implant lab analog, titanium abutment fabricated with a 2-degree tapered axial wall, and zirconia crown. The crown of the CL.F system was retained by frictional force between abutment and relined composite resin. In the cement-retained type, zinc oxide eugenol cement was used to set crown and abutment. All specimens were sterilized with ethylene oxide, immersed in Prevotella intermedia culture in a 50 mL tube, and incubated with rotation. After 48 h, the specimens were washed thoroughly before separating the crown and abutment. The bacteria that penetrated into the crown-abutment interface were collected by washing with 500 µL of sterile saline. The bacterial cell number was quantified using the agar plate count technique. The BacTiter-Glo Microbial Cell Viability Assay Kit was used to measure bacterial adenosine triphosphate (ATP)-bioluminescence, which reflects the bacterial viability. The t-test was performed, and the significance level was set at 5%. RESULTS. The number of penetrating bacterial cells assessed by colony-forming units was approximately 33% lower in the CL.F system than in the cement-retained type (P<.05). ATP-bioluminescence was approximately 41% lower in the CL.F system than in the cement-retained type (P<.05). CONCLUSION. The CL.F system is more resistant to bacterial penetration into the abutment-crown interface than the cement-retained type, thereby indicating a precise marginal fit.

The Effect of Manufacturing Conditions of Coated Yarn Using Anti-Static Thermoplastic Polyurethane M/B on Anti-Static Resistance (대전방지 열가소성폴리우레탄 M/B를 이용한 코팅사 제조 조건이 대전방지성에 미치는 영향)

  • Yedam Jeong;Jieun Kwon;Sunmin Kwon;Seehyeon Chae;Hyunjea Cho;Wooseok Kim;Mikyung Kim;Jongwon Kim
    • Textile Coloration and Finishing
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    • v.35 no.1
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    • pp.20-28
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    • 2023
  • In this study, TPU resin for coating was prepared by varying the mixing ratio of antistatic TPU and recycled TPU to manufacture permanent antistatic materials. The coated yarn was prepared by coating on the nylon yarn, and then the thermal, rheological, mechanical properties and antistatic properties were analyzed. In addition, antistatic properties and durability were confirmed after manufacturing UD fabrics using coated yarns. The mixing ratio of antistatic TPU and recycled TPU was most appropriate at 4:6, and the antistatic property had a surface resistance of 2.20 × 109 Ω and a static charge of 398 V. In the coating process, the coating speed was most appropriate at 0.21 m/s, and the surface resistance of the UD fabric manufactured with the coated yarn manufactured under this condition was 6.80 × 109 Ω and the static charge was 484 V. The UD fabric had a surface resistance of 7.21 × 109 Ω and a static charge of 517 V after washing 10 times, and it was confirmed that the permanent antistatic property was excellent.

Sea water Treatment using an Automatic Backwashing Filter (자동역세척 여과장치를 이용한 해수처리)

  • Park Sang-Ho;Lim Jae-Dong;Kim In-Soo
    • Journal of Navigation and Port Research
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    • v.30 no.3 s.109
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    • pp.241-245
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    • 2006
  • Displayed result that handle particle contaminant and hydrospace organism included a number of ballast that is happened in ship using automatic back washing filter. Reason that removes first contaminant that is included in number of ballast is that heighten processing effectof after processing processof the filter. Another advantage is to drop off the solids with controlling revolution of drum screen in pretreatment filtration process. The fact that it is easy to attach and detach a several type of screen for getting the expected water quality is another advantage. Filter rotation speed of filtration filter could be expose by 35mmHg more than 60 rpm and filter consecutively filtration pressure is 40.5mmHg in 20 rpm and 40 rpm are 36.6mmHg. Filtration system removal aquatic organism over $70{\mu}min$ ballast water. This study shows that the filtration treatment system has a potential for the treatment of ballast water.

Design of RFID-based Integration System for Collection and Recycling Process of EOL Household Electric Appliances in Korea (국내 폐가전제품의 회수 및 재활용을 위한 RFID 기반 통합관리시스템 설계)

  • Kim, Hyun-Soo;Han, Dae-Hee;Jeong, Hae-Jun;Lee, Seong-Hyun
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.32 no.2
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    • pp.120-131
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    • 2009
  • Most world-leading companies are aware that Environment and Health and Safety Issues are critical to the product quality and sustainable growth of their company. Environment-friendly efforts are seen in almost all aspects of business operations in an advanced nation. The Extended Producer Responsibility(EPR) and EU Directive on Waste Electric and Electronic Equipment(WEEE) attempt to tackle the growing quantity of WEEE by making producers responsible for the costs of the collection and recycling of their products at the End-of-Life(EOL). To implement the RFID-based integration system for EOL household electric appliances, such as washing machines and refrigerators, we analyzed the process of collecting, recovering, and recycling the EOL products returned from the distribution points. Furthermore, we proposed a soon-to-be process using the RFID-based integration system in the metropolitan recycling center(MRC). This soon-to-be process model is composed of RFID tags, readers, ALEs, applications and several devices. Through the introduction of the RFID-based integration system, we are expecting to see increasing traceability and real-time management for EOL products from customers, and also improvements in valuable reusable materials(VRM) produced from recycling processes.