• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2003.11a
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• pp.118-126
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• 2003

In the present study, the residual stresses can have a significant on the life of structural engineering components. Residual stresses are created by the surface treatment such as shot peening or deep rolling. The objective of this experimental investigation is to study the influence of friction and wear characteristics due to residual stress under dry sliding condition. Friction and wear data were obtained with a specially designed tribometer. Test specimens were made of SUP9(leaf spring material) after they were created residual stress by shot peening treatment. Residual stress profiles were measured at surface by means of the X-ray diffraction. Sliding tests were carried out different contact pressure and same sliding velocity 0.035m/s(50rpm). Leaf spring assembly test used to strain gauge sticked on leaf spring specimen in order to measure interleaf friction of leaf spring. Therefore, we were obtained hysteresis curve. As the residual stresses of surfaces increased, coefficient of friction and wear volume are decreased, but the residual stresses of surfaces are high, and consequently wear volume do not decreased. Coefficient of friction obtained from leaf spring assembly test is lower than that obtained from sliding test. From the results, structural engineering components reduce coefficient of friction and resistant wear in order to have residual stresses themselves.

• Journal of the Microelectronics and Packaging Society
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• v.9 no.4
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• pp.35-45
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• 2002

The stresses of the underfill/chip interface due to thermal loading was studied using the finite element method. At first, the effective properties of underfill for several volume fractions of silica particles were calculated by Mori-Tanaka method for three different material sets, and the parameters of singularity for the bimaterial edge and the bimaterial wedge were calculated. Consequently, the stresses at the underfill/chip interface with volume fraction of silica particles were investigated. Five different geometric models of flip-chip assembly involving two kinds of bimaterial strips and three kinds of three-layer models were considered under the assumption that the underfill is homogeneous. It was assumed that all components of the flip-chip assembly were linear elastic and isotropic, and their properties were temperature independent. The analysis was conducted in the context of the uncoupled plane thermo-elasticity under a plane strain assumption.

• Journal of Veterinary Science
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• v.24 no.1
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• pp.11.1-11.14
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• 2023

Background: Peripheral blood mononuclear cells (PBMCs) are commonly used to assess in vitro immune responses. However, PBMC isolation is a time-consuming procedure, introduces technical variability, and requires a relatively large volume of blood. By contrast, whole blood assay (WBA) is faster, cheaper, maintains more physiological conditions, and requires less sample volume, laboratory training, and equipment. Objectives: Herein, this study aimed to develop a porcine WBA for in vitro evaluation of immune responses. Methods: Heparinized whole blood (WB) was diluted (non-diluted, 1/2, 1/8, and 1/16) in RPMI-1640 media, followed by phorbol myristate acetate and ionomycin. After 24 h, cells were stained for interferon (IFN)-γ secreting T-cells followed by flow cytometry, and the supernatant was analyzed for tumor necrosis factor (TNF)-α. In addition, diluted WB was stimulated by lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (poly I:C), reference strain KCTC3557 (RS), field isolate (FI), of heat-killed (HK) Streptococcus suis, and porcine reproductive and respiratory syndrome virus (PRRSV). Results: The frequency of IFN-γ⁺CD3⁺ T-cells and concentration of TNF-α in the supernatant of WB increased with increasing dilution factor and were optimal at 1/8. WB TNF-α and interleukin (IL)-10 cytokine levels increased significantly following stimulation with LPS or poly I:C. Further, FI and RS induced IL-10 production in WB. Additionally, PRRSV strains increased the frequency of IFN-γ⁺ CD4^-CD8⁺ cells, and IFN-γ was non-significantly induced in the supernatant of re-stimulated samples. Conclusions: We propose that the WBA is a rapid, reliable, and simple method to evaluate immune responses and WB should be diluted to trigger immune cells.

• Structural Engineering and Mechanics
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• v.88 no.1
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• pp.1-12
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• 2023

In this study, forced vibration behavior of a piezo magneto electric sandwich Timoshenko beam is investigated. It is assumed a sandwich beam with porous core and graphene platelet reinforced composite (GPLRC) in facesheets subjected to magneto-electro-elastic and temperature-dependent material properties. The magneto electro platelets are under linear function along with the thickness that includes a cosine function and magnetic and electric constant potentials. The governing equations of motion are derived using modified strain gradient theory for microstructures. The effects of material length scale parameters, temperature change, different distributions of porous, various patterns of graphene platelets, and the core to face sheets thickness ratio on the natural frequency and excited frequency of a sandwich Timoshenko beam are scrutinized. Various size-dependent methods effects such as MSGT, MCST, and CT on the natural frequency is considered. Moreover, the final results affirm that the increase in porosity coefficient and volume fractions lead to an increase in the amount of natural frequency; while vice versa for the increment in the aspect ratio. From forced vibration analysis, it is understood that by increasing the values of volume fraction and the length thickness of GPL, the maximum deflection of a sandwich beam decreases. Also, it is concluded that increasing the temperature, the thickness of GPL, and the initial force leads to a decrease in the maximum deflection of GPL. It is also shown that resonance phenomenon occurs when the natural and excitation frequencies become equal to each other. Outcomes also reveal that the third natural frequency owns the minimum value of both deflection and frequency ratio and the first natural frequency has the maximum.

• Geomechanics and Engineering
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• v.36 no.1
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• pp.39-50
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• 2024

Bitumen and high-quality subangular aggregates, the two principal materials used for asphalt concrete construction, are finite and expensive materials. The general availability of crumb rubber and naturally occurring aggregates of different shapes, especially flat and elongated shapes, indicates that they are feasible alternative materials for expanding the volume of bitumen and utilizing a wider range of aggregate shapes for the development of asphalt concrete, with an associated environmental benefit. The study investigated the effect of adding up to 15% crumb rubber and aggregates sorted into different groups, i.e., rounded, elongated, flat, and their combinations, on the rheological and mechanical properties and durability of 50/70 of hot-mix asphalt pavement. The addition of crumb rubber decreased ductility and penetration but increased the softening point. For a 5.5% bitumen content, asphalt concrete briquettes consisting of 7% crumb rubber and three types of aggregate shapes, i.e., 100% rounded, a mix of 75% rounded and 25% elongated, and a mix of 75% rounded, 15% elongated and 10% flat, were associated with high Marshall stability and indirect tensile strength as well as low lateral deformation due to their high solidity and moderate angularity ratio. Also, the addition of 7% crumb rubber resulted in a significant improvement in the tensile strength ratio and rebound strain of briquettes consisting of 75% rounded and 25% elongated aggregates and those with 75% rounded, 15% elongated and 10% flat aggregates. In relation to the parameters investigated, the three groups of briquettes met some of the local (South Africa) requirements for the surface course and base course of low traffic volume roads.

• Journal of Microbiology and Biotechnology
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• v.34 no.8
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• pp.1580-1591
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• 2024

Menopause is induced by spontaneous ovarian failure and leads to life quality deterioration with various irritating symptoms. Hormonal treatment can alleviate these symptoms, but long-term treatment is closely associated with breast and uterine cancer, and stroke. Therefore, developing alternative therapies with novel anti-menopausal substances and improved safety is needed. In our study, heat-killed Bifidobacterium breve HDB7040 significantly promoted MCF-7 cell proliferation in a dose-dependent manner under estrogen-free conditions, similar to 17β-estradiol. This strain also triggered ESR2 expression, but not ESR1, in MCF-7 cells. Moreover, administrating HDB7040 to ovariectomized (OVX) Sprague-Dawley (SD) female rats reduced estrogen deficiency-induced weight gain, fat mass, blood triglyceride, and total cholesterol levels. It also recovered collapsed trabecular microstructure by improving trabecular morphometric parameters (bone mineral density, bone volume per tissue volume, trabecular number, and trabecular separation) and decreasing blood alkaline phosphatase levels with no significant changes in uterine size and blood estradiol. HDB7040 also significantly regulated the expression of Tff1, Pgr, and Esr2, but not Esr1 in uteri of OVX rats. Heat-killed B. breve HDB7040 exerts an anti-menopausal effect via the specific regulation of ERβ in vitro and in vivo, suggesting its potential as a novel substance for improving and treating menopausal syndrome.

• Journal of the Korea Concrete Institute
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• v.27 no.5
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• pp.521-530
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• 2015

Design of fiber reinforced ultra-high strength concrete members should be verified with analytical or experimental methods for safety. Members with compressive strength larger than limitation of current design code usually be designed with analytical verification using stress-strain relation of concrete and reinforcements. For this purpose, mechanical characteristics of steel fiber reinforced ultra-high strength concrete were defined under uniaxial compression. Mix proportions of test specimens were based on reactive powder concrete and straight steel fibers were mixed with different volume fraction. Compressive strength of matrix were distributed from 80 MPa to 200 MPa. Effect of fiber inclusion were investigated : increase of compressive strength of concrete, elastic modulus and strain corresponding to peak stress. For the wide range application of investigation, previously tested test specimens were collected and used for investigation and estimation equation. Based on the investigation and evaluation of previous research results and estimation equation of mechanical characteristics of concrete, regression equations were suggested.

• Geomechanics and Engineering
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• v.8 no.1
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• pp.81-95
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• 2015

The permeability behavior of Ariake clays has been investigated by constant rate of strain (CRS) consolidation tests with vertical or radial drainage. Three types of Ariake clays, namely undisturbed Ariake clay samples from the Saga plain, Japan (aged Ariake clay), clay deposit in shallow seabed of the Ariake Sea (young Ariake clay) and reconstituted Ariake clay samples using the soil sampled from the Saga plain, were tested. The test results indicate that the deduced permeability in the horizontal direction ($k_h$) is generally larger than that in the vertical direction ($k_v$). Under odometer condition, the permeability ratio ($k_h/k_v$) increases with the vertical strain. It is also found that the development of the permeability anisotropy is influenced by the inter-particle bonds and clay content of the sample. The aged Ariake clay has stronger initial inter-particle bonds than the young and reconstituted Ariake clays, resulting in slower increase of $k_h/k_v$ with the vertical strain. The young Ariake clay has higher clay content than the reconstituted Ariake clay, resulting in higher values of $k_h/k_v$. The microstructure of the samples before and after the consolidation test has been examined qualitatively by scanning electron microscopy (SEM) image and semi-quantitatively by mercury intrusion porosimetry (MIP) tests. The SEM images indicate that there are more cut edges of platy clay particles on a vertical plane (with respect to the deposition direction) and there are more faces of platy clay particles on a horizontal plane. This tendency increases with the increase of one-dimensional (1D) deformation. MIP test results show that using a sample with a larger vertical surface area has a larger cumulative intruded pore volume, i.e., mercury can be intruded into the sample more easily from the horizontal direction (vertical plane) under the same pressure. Therefore, the permeability anisotropy of Ariake clays is the result of the anisotropic microstructure of the clay samples.

• The Korean Journal of Pesticide Science
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• v.2 no.2
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• pp.113-118
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• 1998

The efficacy of conidia of Beauveria bassiana (Balsamo) Vuillemin strain CS-1 that showed high mortality in laboratory and net house was examined against diamondback moth larvae in the field. Conidia ($4{\times}10^{7}$ conidia/ml) were applied to larvae on chinese cabbage in a 1.5% emulsifiable oil-emulsion amended with 4% clay at a volume of 0.55 L/3.3 $m^{2}$. There were no significant differences among plants and replicates in the deposition of spray droplets on water-sensitive papers or of conidia on leaves and larvae. Weather conditions were rainy and cool during first few days, and then hot and dry. Persistence of conidia was equally short on both leaves and larvae. Nevertheless, treatment of B. bassiana potentially reduced larval populations. But the field efficacy was lower than the laboratory efficacy. This reduced efficacy was ascribed to unfavorable environmental conditions.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.187-191
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• 1998

For efficient and economical production of Btrc,illus tlz~rr.ingi~r1~sstirsa in NT0423 as amicrobial-control agents, a new culture medium and culture condition were developed. Five mediadesignatzd as SWI I , SW14, SW23. SW32 and SW4I were prepared ~ : i t hv arious mixture ratio ofsoybean cake and wheat bran. It was founcl that in terms of the cell growth rate and development ofsporulation of B, thrri.il~girrl.sis strain NT0423 in all SW culture media were more efficient than those inGYS and in LB media. Total cell number in all SW media showed similar values, hut SW32 lnediilm wasthe most efficient in the development of spore, which amo~~ntetod 3.7 x 10XC FUImI. Also. at the pHranging frorn 6.2 to 7.3 in the mediiun~ no ad\:erse effect was not made in the culture of B. thur-ingicnsisstrain NT0423. The optimal volume (%) of SW32 mecliuni in a 5 1 fernientor was determined as 4 8\rolume of total niediuni. resulting ill growth (4.2 x 1OTCFUlrnl) of H. t1~~irir1,yirrz.ssit.vr ain NT0423. As H.t l i ~ t r i t ~ g iw~ a~s~ csuil~tu rcd in the shakc-flash and 5 1 fcrnientor. bacterial cells were yielded to 1 X 10"CFUIml and 5 x I O1oCFLJlml.FUIml and 5 x I O1oCFLJlml.