• The Plant Pathology Journal
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• v.21 no.3
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• pp.262-269
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• 2005

An antiviral producing bacterial strain was isolated from a ginseng rhizosphere in Kangwon province of Republic of Korea. In order to identify the bacterial strain, microbiological, physiological and biochemical tests were performed, along with RAPD, 16S rRNA, 16S-23S rRNA ITS (intergenic spacer region) and DNA-DNA hybridization analyses. The bacterium was found to be a strain of Pseudomonas fluorescens, which was designated as Gpf01. The strain was grown in Muller-Hinton (MH) broth, and the culture supernatant obtained was filtered through a $0.45{\mu}l$ filter. It was further boiled at $100^{\circ}C$ and tested in two experiments for its ability to control a yellow strain of Cucumber mosaic virus (CMV-Y). In the first experiment, boiled culture filtrate (RCF) was treated on one half of the leaves of Chenopodium amaranticolor followed by CMV- Y inoculation on both halves. In the second experiment, BCF was treated on the lower leaves of Nicotiana tobacum var. Xanthi-nc, with the CMV-Y mechanically inoculated onto the upper untreated leaves. In the first experiment, BCF treatment was able to considerably reduce the number of viral lesion, and in the second experiment, plants treated with BCF showed no visible viral symptoms compared to the Muller-Hinton (MH) media treated controls 15 days post inoculation (dpi), and remained symptomless throughout the study period. Thus, Gpf01, identified as P. fluorescence, was able to produce an antiviral component in the culture filtrate, which was found to be heat stable, non-phytotoxic and effective in local as well as systemic hosts of CMV.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.3
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• pp.241-246
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• 2014

Viruses in the genus Megalocytivirus have been subdivided into four subgroups. Among these subgroups 2 and 4, represented by the red sea bream iridovirus (RBIV) and the olive flounder iridovirus (FLIV), respectively, are non-exotic. subgroups 1 and 3, represented by the red sea bream iridovirus (RSIV) and the infectious spleen and kidney necrosis virus (ISKNV), respectively, have not been detected in Korea and are known as exotic. Shellfish are filter-feeders, and can thus filter and accumulate Megalocytivirus in their digestive glands, allowing us to track viral contamination in surrounding aquatic environment. In this study, we developed a high-resolution melting (HRM) analysis to differentiate among subgroups of Megalocytivirus accumulated in shellfish, and confirmed the convenience and efficiency of this method. More than two subgroups of Megalocytivirus were found in the digestive gland of a single shellfish. We classified all Megalocytivirus viruses from shellfish in Korea into subgroups 2 and 4, although proportions of subgroups were different among regions. Compared to nucleotide sequencing analysis, HRM analysis is a simple and rapid method for differentiating of Megalocytivirus subgroups.

• Journal of Life Science
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• v.10 no.4
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• pp.368-373
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• 2000

Enteroviruses in the environment pose a public health risk because they can be transmitted via the fecal-oral route through contaminated water, and low numbers are able to initiate an infection in humans. Because the levels of viruses typically found in environmental water and drinking water are low, they must be concentrated from hundreds to thousands of liters of water. Therefore, the main goal of this study was the development of a rapid, simple and efficient procedure to concentrate, isolate and detect enteroviruses from environmental water samples. Viruses were first concentrated by adsorption to 1 MDS cartridge filter and then eluted with approximately 0.5 liter of 1.5% beef extract/0.05M glycin(pH 9.4). In this study, several procedures to concentrate and purify intact viruses from beef extract obtained from the adsorbent filters were tested. Among them, organic floccuration was the best reliable method for reconcentration. sample volume could be reduced to 200∼400 folds and the efficiency of virus recovery through the procedure was over 72%. Finally, the samples were filtered through a membrane disk filter and then analyzed by either the plaque assay or combined cell culture-polymerase chain reaction.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2001.05a
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• pp.126-130
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• 2001

In this paper, we propose the design methodology of target tracking system using fuzzy basis function expansion (FBFE) based on virus evolutionary genetic algorithm(VEGA). In general, the objective of target tracking is to estimate the future trajectory of the target based on the past position of the target obtained from the sensor. In the conventional and mathematical nonlinear filtering method such as extended Kalman filter (EKF), the performance of the system may be deteriorated in highly nonlinear situation. To resolve these problems of nonlinear filtering technique, by appling artificial intelligent technique to the tracking control of moving targets, we combine the advantages of both traditional and intelligent control technique. In the proposed method, after composing training datum from the parameters of extended Kalman filter, by combining FBFE, which has the strong ability for the approximation, with VEGA, which prevent GA from converging prematurely in the case of lack of genetic diversity of population, and by identifying the parameters and rule numbers of fuzzy basis function simultaneously, we can reduce the tracking error of EKF. Finally, the proposed method is applied to three dimensional tracking problem, and the simulation results shows that the tracking performance is improved by the proposed method.

• Journal of the Korea Safety Management & Science
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• v.4 no.3
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• pp.177-187
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• 2002

The contamination problem is getting worse now a day because of the industrial activities. Recently it has been announced that there's environmental hormone in river and lake which produces clean water, also there's too many of algae reproduces under the water and some virus in the drinking water. The quality of water is very important. pure and clean water is not only a precondition for human being to live but a basic factor to improve the quality of life. so a water purification system must be developed. This study is about the surface washing treatment technique. We tried to use a rotary nozzle to get a tush degree of efficiency, for it was not enough with present way of washing. The nozzle is run by water pressure. The results of the experiment are as follows. We got a clean and equal surface after washing. After washing, the water's maximum consistency was 330NTU and it shows that this way is better than before one with 215NTU. Clean level of the filter was 6$^{\circ}$and it's 2.8 times higher than 17$^{\circ}$with the old way. We can see the results that the new way of washing is more effective than old way based on this study.

• Proceedings of the Membrane Society of Korea Conference
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• 1997.06a
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• pp.207-231
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• 1997

The use household water purifier is continuously increasing due to the distrust of the piped, rap water, which may be caused by the deterioration of water quality and water supply facilities in Korea. The water purifier distributing in the domestic market is the membrane type that composes of membrane as a basic filter, sediment filter as a prefilter and activated carbon filter instead of one purifying water system. The membrane type using as a water cleaning system is mainly the method of micro filtration(NIF) as well as ultra filtration(UF) and reverse osmosis(RO). The types of MF and UF are using to remove suspended solids and virus, and RO is using to remove ionic compound in chemical organic compounds. At the beginning the household water purifier was imported as end-product or assembled by only importing module from small businesses in Korea. Therefore, people was hard to confide in the product quality because the sales policy and management of water purifier by small business were not effectively organized. However, the defects of water purifier having up to now ate improving by the efforts of special branches of water purifier and large enterprises, and new great products are developing and producing. To develop the best quality product of water purifier in Korea, it should be considered seasonal water characteristrics rather than any other condition. For example, the water purifier can be affected by the water temperature change, increase of suspended solids in the rainy season and inflow of raw water having concentration in a dry season. The subject we have to investigate in the future is to develop the water purifying system adaptable in Korea which can treat the water quality using close analyses of local and seasonal water characteristics.

• 대한예방의학회:학술대회논문집
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• 1995.10a
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• pp.123-124
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• 1995

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.1
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• pp.17-30
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• 2015

Unlike existing widely used bytecode-centric Android application code obfuscation methodology, our scheme in this paper makes encrypted file i.e. DEX file self-extracted arbitrary Android application. And then suggests a method regarding making the loader app to execute encrypted file's code after saving the file in arbitrary folder. Encrypted DEX file in the loader app includes original code and some of Manifest information to conceal event treatment information. Loader app's Manifest has original app's Manifest information except included information at encrypted DEX. Using our scheme, an attacker can make malicious code including obfuscated code to avoid anti-virus software at first. Secondly, Software developer can make an application with hidden main algorithm to protect copyright using suggestion technology. We implement prototype in Android 4.4.2(Kitkat) and check obfuscation capacity of malicious code at VirusTotal to show effectiveness.

• The KIPS Transactions:PartC
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• v.8C no.6
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• pp.741-756
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• 2001

With the development of computer systems, there has been a sharp increase in the threats on these systems including attacks by malicious programs such as virus, vandal, etc. Currently virus vaccines are widely used to thwart these threats, but they have many weaknesses. They cannot guard against unknown threats and sometimes, they also cannot detect the existence of malicious programs before these malicious programs make any destructive results. For lack of an efficient security model, the existing security programs have the problem that they raise many false-positive alarms in spite of normal action. So it becomes very important to develop the improved security program that can make up for the weakness of the existing computer security program and can detect many threats of malicious programs as early as possible. In this paper we describe the design of an improved security model and the implementation of a security program that can filter and handle the threats on computer systems at the kernel level in real time.

• Journal of Environmental Health Sciences
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• v.48 no.2
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• pp.116-122
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• 2022

Background: The recent COVID-19 pandemic is one of the worst disease outbreaks of the 21th century. Due to a lack of reliable antiviral therapeutics, wearing face masks is recommended to prevent airborne infection originating from virus-contaminated bioaerosols. Objectives: The aim of this study was to evaluate the filtration efficiencies of face masks that are commercially available in South Korea for a biological aerosol of Staphylococcus aureus (S. aureus) and murine coronavirus, a well-known surrogate for human coronaviruses. Methods: We collected six different kinds of commercial masks: two Korea Filter (KF)94 (KF94-1, KF94-2) masks, one surgical (Surgical-1) mask, one anti-droplet (KF-AD-1) mask, and two dust (Dust-1, Dust-2) face masks. S. aureus (ATCC 6538), a well-performing test bacteria and murine coronavirus (ATCC VR-764) were prepared under a suitable culture condition. Then, a mask biological filtration tester was used to examine the microbial filtration efficiencies of masks. Test microorganisms were quantitatively measured via cultivation methods and microbial filtration efficiencies were calculated appropriately. Results: All face masks showed over 99.6% filtration efficiency for S. aureus or murine coronavirus. There were no significant differences among the bacterial filtration efficiencies of the face masks. KF94-1 (99.97±0.08%) and Dust-1 mask (99.97±0.07%) showed the highest (over 99.9%) filtration efficiency for murine coronavirus. KF94-1 or Dust-1 masks showed a significant virus filtration efficiency compared to Surgical-1 mask (p<0.05; Mann-Whitney U test). Conclusions: All the commercially available face masks used in this study can filter S. aureus or murine coronavirus in bioaerosols efficiently, regardless of the mask type. Therefore, our results suggest that wearing a certified face mask is a reliable means to prevent the transmission of infectious airborne diseases via biological aerosols.