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Alternative Isoforms of TonEBP with Variable N-termini are Expressed in Mammalian Cells

  • Kim, Hyo-Shin;Son, Sook-Jin;Kim, Seon-Nyo;Kim, Yong-Duk;Kim, Kwang-Jin;Jeon, Byeong-Hwa;Park, Jin-Bong;Lee, Sang-Do
    • The Korean Journal of Physiology and Pharmacology
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    • v.11 no.3
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    • pp.135-138
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    • 2007
  • Hypertonicity imposes a great deal of stress to cells since it causes rise in cellular ionic strength, which can be reduced by the accumulation of compatible osmolytes. TonEBP plays a central role in the cellular accumulation of compatible osmolytes via transcriptional stimulation of membrane transporters and aldose reductase. Alternatively spliced forms of TonEBP mRNA have previously been reported and two of them showed different transcriptional activity. In the present study, isoform-specific antibodies were produced to confirm the translation of the spliced mRNA to protein. TonEBP was immunoprecipitated by using anti-TonEBP antibody and then immunoblotted using anti-TonEBP or isoform specific antibodies to find out the expression profile of TonEBP isoforms in basal or stimulated condition. From these results, we conclude that all TonEBP isoforms are expressed in mammalian cells and their expression patterns are not same in every cells.

TREATMENT OF CLASS II MALOCCLUSION IN THE MIXED DENTITION WITH CLASS II ACTIVATOR: CASE REPORT (II급 Activator를 이용한 혼합치열기 II급 부정교합아동의 치험례)

  • Yoo, Kun-Jung;Kim, Hyun-Jung;Nam, Soon-Heun;Kim, Young-Jin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.4
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    • pp.735-742
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    • 1997
  • Class II malocclusion can be treated via early orthopedic, orthodontic treatment or orthognathic surgery with orthodontic treatment. In the mixed dentition, early orthopedic treatment can be used. Especially, in the case of mandibular retrognathism, the functional appliances can be used, and in the case of maxillary protrusion is combined, they can be used together with headgear. After using activator and activator combined with headgear to the class II malocclusion paitent in the mixed dentition, the results were as follows: 1. Lateral profile was improved, and lower face height was increaed. 2. Overjet was decreased, and molar relationship was changed to class I molar relationship. 3. Growth can be undisturbed, and the aggravation of malocclusion can be prevented to make the 2nd phase orthodontic treatment be much easier.

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Development of Gear Stiffness Module for Multi-Body Dynamic Analysis on Gears (다물체 동역학 해석을 위한 기어 강성 모듈 개발)

  • Song, Jin-Seop;Lee, Geun-Ho;Park, Young-Jun;Bae, Dae-Sung;Lee, Chul-Ho
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.21 no.1
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    • pp.130-136
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    • 2012
  • Dynamic as well as static and geometric design parameters such as inertia, tooth profile, backlash and clearance can be directly considered via multi-body dynamic analysis along with contact analysis. However, it is time consuming to use finite elements for the consideration of the tooth flexibility in the multi-body dynamic analysis of gears. A computationally efficient procedure, so called, Gear Stiffness Module, is suggested to resolve this calculation time issue. The characteristics of gear tooth compliance are discussed and rotational stiffness element concept for the Gear Stiffness Module is presented. Transmission error analyses for a spur gear system are carried out to validate the reliability and efficiency of the module. Compared with the finite element model, the Gear Stiffness Module yields considerably similar results and takes only 3% of calculation time.

Ab Initio Study of Mechanism of Forming Spiro-Heterocyclic Ring Compound Involving Si and Ge from Dichlorosilylene Germylidene (Cl2Si-Ge:) and Acetone

  • Liu, Dongting;Ji, Hua;Lu, Xiuhui
    • Bulletin of the Korean Chemical Society
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    • v.33 no.12
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    • pp.4079-4083
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    • 2012
  • The mechanism of the cycloaddition reaction between singlet state dichlorosilylene germylidene ($Cl_2Si=Ge:$) and acetone has been investigated with B3LYP/6-$31G^*$ and B3LYP/6-$31G^{**}$ method, from the potential energy profile, we predict that the reaction has one dominant reaction pathway. The presented rule of the reaction is that the two reactants firstly form a Si-heterocyclic four-membered ring germylene through the [2+2] cycloaddition reaction. Because of the 4p unoccupied orbital of Ge atom in the Si-heterocyclic four-membered ring germylene and the ${\pi}$ orbital of acetone forming a ${\pi}{\rightarrow}p$ donor-acceptor bond, the Si-heterocyclic four-membered ring germylene further combines with acetone to form an intermediate. Because the Ge atom in the intermediate hybridizes to an $sp^3$ hybrid orbital after the transition state, then, the intermediate isomerizes to spiro-heterocyclic ring compound involving Si and Ge (P4) via a transition state.

A Nonlinear Observer Design for Estimating State-of-Charge of Lithium Polymer Battery (리튬폴리머 배터리 잔존충전용량 추정을 위한 비선형 관측기 설계)

  • Yoo, Seog-Hwan
    • Journal of the Korean Institute of Intelligent Systems
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    • v.22 no.3
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    • pp.300-304
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    • 2012
  • This paper presents a nonlinear observer design method for SOC(state-of-charge) estimation of Lithium polymer battery cell. The dynamic equation of the battery cell is modeled as a simple RC electrical circuit with a nonlinear voltage source and the parameters are obtained via nonlinear optimization. Using the sum of squares decomposition, the observer gain is designed such that the error dynamics is asymptotically stable and the decay rate is below the prescribed value. In order to illustrate the performance of the observer, a computer simulation is performed using the experimental data with the UDDS(urban dynamometer driving schedule) current profile.

A Study on the Simulation Model of PV Generation System for its Application to Real Power System (계통 연계형 태양광 발전 시스템의 실계통 적용을 위한 시뮬레이션 모델에 관한 연구)

  • Lee, Kye-Byung;Son, Kwang-Myung;Jeon, In-Soo
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.22 no.6
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    • pp.70-78
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    • 2008
  • This paper deals with the simulation modeling of PV generation system for its application to utility distribution network PSCAD/EMIDC simulation model is developed for use in studying the effect of the PV generation to the distribution system. Simulation results show that the addition of the PV system improves the voltage profile of the area by decreasing the power flow from the utility substation. Case studies also show that power quality at the load side is also improved via voltage compensation at the load bus.

Dynamic SOC Compensation of an Ultracapacitor Module for a Hybrid Energy Storage System

  • Song, Hyun-Sik;Jeong, Jin-Beom;Shin, Dong-Hyun;Lee, Baek-Haeng;Kim, Hee-Jun;Heo, Hoon
    • Journal of Power Electronics
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    • v.10 no.6
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    • pp.769-776
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    • 2010
  • The ultracapacitor module has recently been recast for use in hybrid energy storage systems (HESSs). As a result, accurate state-of-charge (SOC) estimation for an ultracapacitor module is as important as that of primary sources in order to be utilized efficiently in an energy storage system (ESS). However, while SOC estimation via the open-circuit voltage (OCV) method is generally used due to its linear characteristics compared with other ESSs, this method results in many errors in cases of highcurrent charging/discharging within a short time period. Accordingly, this paper introduces a dynamic SOC estimation algorithm that is capable of SOC compensation of an ultracapacitor module even when there is a current input and output. A cycle profile that simulates the operating conditions of a mild-HEV was applied to a vehicle simulator to verify the effectiveness of the proposed algorithm.

Analysis of Consumer Preferences for Cosmetic Essence-for-Men via Choice-Based Conjoint with New Design of Choice Sets

  • Kim, Bu-Yong;Kim, Jiyoung;Kan, Yu-Yeong
    • The Korean Journal of Applied Statistics
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    • v.25 no.6
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    • pp.987-997
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    • 2012
  • The sales volume of men's cosmetics has drastically increased in Korea. In recent years, men's needs for cosmetics have been diversified and the consumer demand for functional cosmetics has greatly risen. In particular, male consumers have become more interested in essence product that is a light and concentrated treatment to correct skin problems. This research analyzes consumer preferences for essence-for-men through the use of choice-based conjoint analysis. This approach is adopted since the task of respondents to choose the most preferred option from several alternatives closely mimics actual marketplace purchasing behavior by consumers. New technique for the construction of choice sets is suggested based on the balanced incomplete block design, to accommodate a larger number of product profiles. The proposed design for choice sets is balanced and provides a tool to filter the contradictory choices. Conjoint analyses are performed to assess the relative importance of attributes and identify the most preferred profile of essence-for-men with respect to attributes such as emphasized function, price, type of content, and design of container. Some differences are indicated in the analysis results between age brackets as well as between groups classified by the amount of fashion item expenditures.

Development of m-Health Application based on Medical Informatics Standards (의료정보 표준에 기반 한 m-Health 어플리케이션 개발)

  • Park, Hyun Sang;Kim, Hwa Sun;Jung, Hyun Jung;Cho, Hune
    • Journal of Korea Multimedia Society
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    • v.17 no.5
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    • pp.640-653
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    • 2014
  • Recently medical consumers pursue high leveled quality of life through active promotion, prevention, management activity of health as the importance of health during home life is emerged to meet the aging society and chronic diseases. In addition, people are directed to the m-Health for managing yourself their health by using smartphone and various personal health devices, if necessary transmits health data to the physician himself. In the previous studies, m-Health Application were developed and applied in the real clinical environment by adopting the medical information standards was rarely conducted. Therefore, in this study, the m-Health application platform was developed. Developed application was communicate with IEEE 11073 standard protocol using the Bluetooth Health Device Profile in personal health device via smart phone to process blood pressure information, and it converted to HL7 V2.6 ORU_R01 message for send to remote medical server. In addition, we tested the interoperability and safety of the developed application for 23 inpatient and 17 outpatient at D University Hospital. As a result, the blood pressure information has been transmitted without error.

Application the mechanism-based strain gradient plasticity theory to model the hot deformation behavior of functionally graded steels

  • Salavati, Hadi;Alizadeh, Yoness;Berto, Filippo
    • Structural Engineering and Mechanics
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    • v.51 no.4
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    • pp.627-641
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    • 2014
  • Functionally graded steels (FGSs) are a family of functionally graded materials (FGMs) consisting of ferrite (${\alpha}$), austenite (${\gamma}$), bainite (${\beta}$) and martensite (M) phases placed on each other in different configurations and produced via electroslag remelting (ESR). In this research, the flow stress of dual layer austenitic-martensitic functionally graded steels under hot deformation loading has been modeled considering the constitutive equations which describe the continuous effect of temperature and strain rate on the flow stress. The mechanism-based strain gradient plasticity theory is used here to determine the position of each layer considering the relationship between the hardness of the layer and the composite dislocation density profile. Then, the released energy of each layer under a specified loading condition (temperature and strain rate) is related to the dislocation density utilizing the mechanism-based strain gradient plasticity theory. The flow stress of the considered FGS is obtained by using the appropriate coefficients in the constitutive equations of each layer. Finally, the theoretical model is compared with the experimental results measured in the temperature range $1000-1200^{\circ}C$ and strain rate 0.01-1 s-1 and a sound agreement is found.