• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.193-193
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• 2004

Gene targeting is an in situ manipulation of endogenous gene with precise manner by the introduction of exogenous DNA. The process of gene targeting involves a homologous recombination reaction between the targeted genomic sequence and an exogenous targeting vector. In elucidating the function of many genes, gene targeting has become the most important method of choice. (omitted)

• Proceedings of the KIPE Conference
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• 2017.11a
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• pp.41-42
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• 2017

기존의 다중 코일을 이용한 소형 자성체를 제어하는 시스템에서 전류 벡터 지령은 구동 매트릭스의 pseudo-inverse를 사용하여 계산되었다. 본 논문에서는 기존의 전류 지령에 null space 전류 벡터 지령을 더하는 방법을 이용하여 현재 전류 벡터값과 목표 전류 벡터 지령 사이의 전류 변화량이 최소화 되는 전류 지령 수정 방식을 제안한다. 제한된 전압 정격 내에서 제안된 방식을 적용할 경우 전류 지령 벡터값에 도달하는 시간이 짧아짐으로, 기존 방식에 비해 빠른 반응성을 얻을 수 있다. 제안된 방법은 Matlab(R) Simulink(R)를 통해 검증하였다.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.32 no.1
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• pp.62-69
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• 2008

A post-processing program based on the OOP(Object-Oriented Programming) concept has been developed for flow visualization of the flow analysis code(PowerCFD) using unstructured cell-centered method. User-friendly GUI(GTaphic User Interface) has been built on the base of MFC(Microsoft Foundation Class). The program is organized as modules by classes including those based on VTK(Visualization ToolKit)-library, and these classes are made to function through inheritance and cooperation which is an important and valuable OOP concept. The major functions of this post-processor program are introduced and demonstrated, which include mesh plot, contour plot, vector plot, surface plots, cut plot, clip plot, xy-plot and streamline plot as well as view manipulation (translation, rotation, scaling etc).

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.4
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• pp.459-466
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• 1994

Primordial germ cells (PGCs) in aves are the progenitor cells for the gametes. These cells first appear in the epiblast (Eyal-Giladi et al.. 1981). Then translocate and concentrate to endoderm of germinal crescent area in the junction of the area opaca and area pellucida lateral to the primitive streak in stage 4 through 7. They separate from the endoderm, temporarily circulate via the blood vascular system, leave the blood vessels, and finally settle down in the gonadal anlagen at stage 20-24 where they rapidly proliferate to form germ cells. Recently, several attempts have been made to introduce foreign gene into the avian genome to form a transgenic chicken. The stem cells most readily available as vehicles for genetic manipulation of germline in avian species are the PGCs. PGCs have recently been manipulated genetically and used successfully as a vector for gene transfer.

• Korean Journal of Computational Design and Engineering
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• v.12 no.2
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• pp.126-136
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• 2007

Teleconferences have been used in business sectors to reduce traveling costs. Traditionally, specialized telephones that enabled multiparty conversations were used. With the introduction of high speed networks, we now have high definition videos that add more realism in the presence of counterparts who could be thousands of miles away. This paper presents a new technology that adds even more realism by telecommunicating with hand gestures. This technology is part of a teleconference system named SMS (Smart Meeting Space). In SMS, a person can use hand gestures to manipulate meeting data that could be in the form of text, audio, video or 3D shapes. Fer detecting hand gestures, a machine learning algorithm called SVM (Support Vector Machine) has been used. For the prototype system, a 3D interaction environment has been implemented with $OpenGL^{TM}$, where a 3D human skull model can be grasped and moved in 6-DOF during a remote conversation between distant persons.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.3
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• pp.427-434
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• 1994

The primordial germ cells (PGCs) were transfected in vitro and expressed the exogenous RSVLTR/${\beta}G2$ plasmid, suggesting thaI PGC is a possible vector for direct gene transfer into the germ line. Transfection efficiency of cell suspensions containing PGCs was 1.5% by liposome mediated DNA transfection. By microinjection of the transfected PGCs into the host germinal crescent, PGCs migrated via blood vessel to the future gonad and these transfected PGCs resulted in the RSVLTR/${\beta}G2$ expression in the gonad. The results from the seeding of PGCs on the chorioallantoic membrane were insufficient to test the hypothesis that PGCs can penetrate or invade the chorioallantoic membrane for transport via the circulatory system.

• Korean Journal of Optics and Photonics
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• v.12 no.6
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• pp.437-445
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• 2001

We examine theoretically the properties of the force on Mie particles induced by evanescent fields at a system of multilayer films (including a metal film), at which the surface plasmon resonance is excited by a p-polarized plane electromagnetic wave. An expression of the surface plasmon-coupled evanescent fields produced in Kretschmann (or Sarid) geometry is expanded in terms of vector spherical wave functions, while multiple reflections between the Mie particle and the metal boundary are taken into account. The Cartesian components of the force on Mie particles by the evanescent fields are analytically formulated and numerically evaluated. The force components are increased by one or two orders of magnitude at metal boundaries over those at dielectric boundaries. As a result, we can confirm the possibility of stable manipulation or rotation of a finite-sized object by forces of surface plasmon-coupled evanescent fields.

• Proceedings of the KSRS Conference
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• 2009.03a
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• pp.82-86
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• 2009

원격탐사 근적외선(NIR)과 Red 밴드의 반사도로부터 계산되는 정규식생지수(NDVI)는 구름에 오염된 곳에서는 실제보다 낮은 값으로 계산된다. 식생지수에서 구름오염 문제를 극복하는 기존의 대표적인 방법에는 보름 정도 장기간 식생지수 값 중에서 최대인 값을 취하는 MVC(Maximum Value Composite) 방법이 있다. 하지만 MVC 방법으로는 식생지수의 단기간 변동을 파악할 수 없으며, 장기간 계속 구름으로 오염된 곳은 잘못된 식생지수 값으로 계산되는 문제점이 있다. 가시광 RGB 자료로부터 snapshot 영상자료의 구름을 마스크(mask)하는 새로운 방법인 CIM(Color Index Manipulation) 알고리즘을 개발하였다. 이 알고리즘을 사용하면 snapshot 영상자료에서 구름에 오염된 곳은 제외하고 오염되지 않은 곳에 대한 식생지수를 계산할 수 있다. RGB 자료에 대한 정규색상지수 NCI (Normalized Color Index) 3개 성분을 $120^{\circ}$ 간격으로 벌어진 3개 축상의 좌표로 나타낸 후 이들 3개 값의 벡터합(vector sum) 정보를 이용하여 구름을 식별하는 CIM 방법으로 위성영상에서 두꺼운 구름과 않은 구름을 구분하여 식별할 수 있다. 이 구름식별 기법을 MODIS snapshot 위성영상 자료에 적용하여 한반도의 일별(daily) 식생지수 자료를 계산하였다. 그리고 수년간의 일별 식생지수 자료로부터 한반도 식생지수의 계절적 변동을 조사하였다.

• Journal of the Korean Institute of Telematics and Electronics
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• v.23 no.2
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• pp.197-205
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• 1986

In this paper, we present an efficient test algorithm for data processing function of MC68000 \ulcorner. The test vector for functional testing is determined by stuck-at, coupling and transition fault for data storage and transfer. But for data manipulation it is determined by a boolean function of micro-operation. This test algorithm is composed of 3 parts, choosing optimum test instructions for maximizing fault coverage and minimizing test process time, deciding the test order for minimizing test ambiguity, and processing the actual test.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.745-753
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• 2008

Gene-manipulated mice were discovered for the first time about a quarter century ago. Since then, numerous sophisticated technologies have been developed and applied to answer key questions about the fundamental roles of the genes of interest. Functional genomics can be characterized into gain-of-function and loss-of-function, which are called transgenic and knock-out studies, respectively. To make transgenic mice, the most widely used technique is the microinjection of transgene-containing vectors into the embryonic pronucleus. However, there are critical drawbacks: namely position effects, integration of unknown copies of a foreign gene, and instability of the foreign DNA within the host genome. To overcome these problems, the ROSA26 locus was used for the knock-in site of a transgene. Usage of this locus is discussed for the gain of function study as well as for several brilliant approaches such as conditional/inducible transgenic system, reproducible/inducible knockdown system, specific cell ablation by Cre-mediated expression of DTA, Cre-ERTM mice as a useful tool for temporal gene regulation, MORE mice as a germ line delete and site specific recombinase system. Techniques to make null mutant mice include complicated steps: vector design and construction, colony selection of embryonic stem (ES) cells, production of chimera mice, confirmation of germ line transmission, and so forth. It is tedious and labor intensive work and difficult to approach. Thus, it is not readily accessible by most researchers. In order to overcome such limitations, technical breakthroughs such as reporter knock-in and gene knock-out system, production of homozygous mutant ES cells from a single targeting vector, and production of mutant mice from tetraploid embryos are developed. With these upcoming progresses, it is important to consider how we could develop these systems further and expand to other animal models such as pigs and monkeys that have more physiological similarities to humans.