• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.248-254
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• 2008

Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources. For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C. magnoliae. Immunoblot analysis with the Pma1 antibody and two-dimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S. pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.

• Journal of Nutrition and Health
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• 제8권3호
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• pp.19-29
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• 1975

This study was designed to compare the metabolic effects of varicous types of Korean diet. 40 males and same number of females of Albino rats, divided into eight groups, 5 rats each. 1 Group; Sugar casein standard Group 2 Group; Rice casein standard Group 3 Group; Rice group 4 Group; 65% Rice +35% Anchovy 5 Group; 65% Rice +35% Chinese cabbage 6 Group; 65% Rice+10% Anchovy +25% Chinese cabbage 7 Group; 65%. Rice+26% Chinese cabbage+3.5% Anchovy+4% Bean+1% Potatoes+0.2% Sea Weeds+0.3% Seasonings 8 Group; 47% Rice+24% Chinese cabbage+20.5% Anchovy+5% Bean+1% Potatoes+1.8% Sea Weeds+0.7% Seasonings The rats were kept in individual cage and given 8 different diet for 10 weeks. The result of this study were elucidated as fallow. Food intake of sugar casein standard group and rice casein standard group and Seoul diet pattern group were high, Rice diet group showed low food intake. F.E.R, P.E.R, body weight, organ weight were the similar results. The nitrogen content in various organs were no great difference, but nitrogen metabolism and total nitrogen retention were significant differences. The lipid content in the liver showed no significant differences, but fecal lipid and serum cholesterol showed significant differences. This study showed the glucose content in urine and feces were due to the dietary carbohydrate content. In other word, the results of this study showed no significant differences between sugar casein standard group and rice casein standard group, but significiant differences between standard group and experimental group.

• 한국보건간호학회지
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• 제16권2호
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• pp.436-444
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• 2002

This study surveyed 146 obese elementary school children(94 male and 52 female) in Seoul, and measured height, body weight, degree of obesity, blood pressure, fasting blood sugar, serum lipid profiles and liver function. The hypertension was above 95 percentile depending on age and sex. and the hyperglycemia was defined as above 110mg/dl. The hypercholesterolemia and hypertriglyceridemia were defined as above 200mg/dl, 160mg/dl, repectively. The abnormal liver function was considered as elevated alanine aminotransferase(ALT>35IU/L)or aspartate aminotransferase(AST>35IU/L). The results were as follows: 1. The mean degree of obesity was $29.56\pm12.56\%$, and the percentage of overweight was $18.5\%$, mild obesity $41.1\%$, moderate obesity $35.6\%$, severe obesity $4.8\%$ respectively in surveyed children. 2. The systolic blood pressure was $108.34\pm13.73mmHg$, diastolic blood pressure was $67.46\pm8.27mmHg$. 3. FBS was $93.79\pm6.51mg/dl$. 4. Total cholesterol, triglyceride, ALT and AST were $183.34\pm31.38mg/dl$. $115.55\pm56.43mg/dl,\;24.08\pm18.42IU/L,\; 28.73\pm10.45IU/L,$ respectively. 5. The prevalence of complications was $47.2\%$ : hypertension$(13\%)$, hyperglycemia$(0.7\%)$, hypercholesterolemia$(23.3\%)$, hypertriglyceridemia$(17.1\%)$, and liver dysfunction$(21.9\%)$. In conclusion. childhood obesity is associated with various risk factors. Therefore, the aggressive approaches to successful prevention, early detection, and effective treatment of obesity in children are urgently required.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.II
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• pp.388-395
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• 2000

This study was performed to examine the effects of infrared (IR)/heated-air combination drying on some quality attributes of Korean white ginsengs. Ginseng roots were dried in a dryer where both the far infrared ray and heated-air are available as drying energy sources. Diametral shrinkage, external color, total saponin content, and ginsenosides and free sugar composition of the IR/heated-air combination dried ginsengs were measured and compared with those of commercial white ginseng products. The external color became lower in lightness and higher in saturation as the IR radiating plate temperature increased. IR/heated-air combination dried white ginsengs at IR plate temperature of 100$^{\circ}C$ was comparable to the commercial white ginseng products in color characteristics. Diametral shrinkage ratios ranged from 20 to 36% and appeared to be independent on the different drying methods. No definite evidence could be found whether the IR/heated-air combination drying and the conventional. hot-air drying practice resulted in white ginsengs having different ginsenoside contents and compositions. No conclusion could be made on whether the various drying treatments used in the study had effects on the free sugar contents and compositions of white ginsengs.

• 한국식품조리과학회지
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• 제26권2호
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• pp.138-145
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• 2010

This study was conducted to develop a novel tomato sauce using natural seasoning. The data was analyzed using SPSS for Windows (version 14.0). Two factors of the tomato sauce were varied was tomato paste and sugar. Nine tomato sauces were produced with various compounding ratios of tomato paste and sugar. The color, viscosity and sensory evaluations of the nine tomato sauces were determined. Items examined in the sensory evaluation were color, sweetness, viscosity and overall quality. Sweetness($4.44{\pm}1.36$), viscosity($5.16{\pm}1.28$) and overall quality($4.68{\pm}1.11$) of the tomato paste 340g and sugar 100 g sample got highest scores. Therefore, this composition was used as tomato spaghetti sauce. Tomato spaghetti sauce using a natural tomato sauce and artificial tomato sauce was prepared and subjected to a sensory evaluation. Items included in the sensory evaluation were taste, flavor, harmony, overall quality. In this analysis, all sensory item scores of the tomato spaghetti sauce using natural seasoning were significantly higher than when artificial seasoning was used. Therefore, the potential of commercialization of tomato sauces using natural seasoning was confirmed.

• 한국식생활문화학회지
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• 제9권2호
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• pp.131-136
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• 1994

한국산 씀바귀(Lactuca dentata Makino)의 ethylacetate 추출물에서 6 종류의 flavonoid 성분이 TLC에 의해서 검출 되었으며, 이들은 극성에 따라서 A에서 F가지 명명되었다. 그들 중 주성분으로 생각되는 물질 E를 column chromatography을 이용하여 분리정제 하였다. 물질 E의 녹는 점은 $249.5^{\circ}C-251^{\circ}C$ 로 나타났으며, 그 구조를 밝히기 위하여, 물질 E와 이를 가수분해한 genin에 대한 여러가지 shift reagent에서 UV와 IR분석을 하고 또한 물질 E와 그의 acetyl 유도체에 대한 NMR 분석을 하였으며 물질 E에 결합된 당은 TLC에 의하여 확인하였다. 이와같은 분석 결과에서 물질 E는 luteolin 모핵에 glucose가 결합된 $luteolin-7-0-{\beta}-D-glucoside$로서 확인되었다.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.497-504
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• 2017

The aim of this study was to investigate the characteristics of paprika dried by various methods. Moisture content of dried paprika was higher in vacuum freeze-dried red paprika (DRP) (11.85%) than in vacuum freeze-drying of osmotic dried red paprika in sugar (RPS), vacuum freeze-drying of osmotic dried red paprika in fructose (RPF) and vacuum freeze-drying of osmotic dried red paprika in corn syrup (RPCS). Carbohydrate content of DRP was the lowest among the dried groups, but fat, protein, and ash contents were the highest in DRP. The pH of paprika was the highest in RPF (5.34), while it was the lowest in DRP (5.05). Reducing sugar and sugar contents of RPF were 28.59 g/100 g and $5.03^{\circ}Brix$, respectively, which are the highest level among the groups. All color values in inside of paprika were the highest in RPCS, while in outside of paprika, L value is the highest in RPCS, and the value of a, b were the highest in RPS. Regarding the texture characteristics of paprika, strongness, hardness, adhesiveness, chewiness and brittleness were the highest in RPS (p<0.05).

• 농업과학연구
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• 제36권1호
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• pp.41-50
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• 2009

Fucoidan is a uniquely-structured sulfated fucose-rich polysaccharide derived from brown algae. Recently, the abalone glycosidase-digested fucoidan extract (fucoidan extract) derived from seaweed Cladosiphon novae-caledoniae Kylin (Mozuku) draws much attention because of its clinical anti-cancer effect in Japan. Here, we report the cancer cells-specific apoptosis inducing effects of the fucoidan extract. The fucoidan extract suppressed the growth of various anchorage-dependent and -independent cancer cells. The fucoidan extract contained low molecular weight components, which induced apoptosis of human leukemic HL 60 cells but not of human lymphocytes. It was shown that the fucoidan extract lead caspase 3/7 activation and loss of mitochondrial membrane potential in HL 60 cells. Another function of the fucoidan extract was also observed. It has been known that sugar chain expression on the surface of cancer cell membrane changes dependent on their malignancy. The analysis on sugar chain expression profiling using FITC-labeled lectins revealed that the expression of concanavalin A (Con A) binding sugar chain was enhanced by the treatment of human lung adenocarcinoma A549, human uterine carcinoma HeLa and human fibrosarcoma HT1080 cells with the fucoidan extract. Con A-induced apoptosis of cancer cells was stimulated in a dose-and time-dependent manner by the treatment with the fucoidan extract but not of human normal fibroblast TIG-1 cells.

• 한국식품저장유통학회지
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• 제14권5호
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• pp.469-473
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• 2007

비동결 파운드 케이크의 배합비를 반응표면분석법에 의해 여러 가지 동결방지제의 함량을 조절하여 결정하였다. 수분함량만이 동결방지제 함량과 선형관계를 나타내었으나, 다른 반응변수들은 quadratic모형으로 나타났다. 비동결 파운드 케이크에 제조시 함유되는 당류의 최적 배합비는 7736 : 0.0 : 20.4, 60.0 : 32.8 : 16.4, 70.8 : 11.2 : 19.6와 같이 3가지로 제시되었다. 최적배합비로 제조한 파운드 케이크의 냉동 시간은 기준 배합비로 만든 케이크보다 $45{\sim}27%$가 단축되었으며, 동결점은 $-20^{\circ}C$이하에서 나타났다. 동결방지제의 적절한 배합율을 적용하면 빙점강하에 의해 가공식품 냉동에 필요한 냉동 에너지 감소 효과를 얻으며 기존제품과 유사한 품질 특성을 구할 수 있음을 알았다.

• 한국식생활문화학회지
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• 제4권4호
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• pp.411-416
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• 1989

적채시기별 녹차의 침출조건에 따른 유리당, 총질소 및 탄닌함량은 침출온도$(55{\sim}95^{\circ}C)$가 높을수록 침출시간(30초${\sim}$10분)이 길수록 증가하였다. 침출조건에 따른 녹차의 관능검사 결과 유리당 및 총 질소는 유의성 있는 상관관계가 나타나지 않았으나 탄닌함량과는 $15{\sim}30mg/g$의 농도에서 변곡점을 형성하는 유의성 있는 상관관계(r=-0.9061)을 나타내었다. 탄닌함량과 관능검사에 의한 녹차의 최적 침출조건은 상급차의 경우 낮은 온도$(55{\sim}75^{\circ}C)$에서 단시간 ($0.5{\sim}1$분)이었고 중급차는 $85^{\circ}C$ 이하에서 2분 이내가 최적 조건이었다.