• Journal of Plant Biotechnology
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• 제43권4호
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• pp.457-465
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• 2016

Microsatellites are one of the most suitable markers for identification of variety, as they have the capability to discriminate between narrow genetic variations. The polymorphism level between 120 microsatellite primer pairs and 148 soybean varieties was investigated through the fluorescence based automatic detection system. A set of 16 primer pairs showed highly reproducible polymorphism in these varieties. A total of 204 alleles were detected using the 16 microsatellite markers. The number of alleles per locus ranged from 6 to 28, with an average of 12.75 alleles per locus. The average polymorphism information content (PIC) was 0.86, ranging from 0.75 to 0.95. The unweighted pair group method using the arithmetic averages (UPGMA) cluster analysis for 148 varieties were divided into five distinctive groups, reflecting the varietal types and pedigree information. All the varieties were perfectly discriminated by marker genotypes. These markers may be useful to complement a morphological assessment of candidate varieties in the DUS (distinctness, uniformity and stability) test, intervening of seed disputes relating to variety authentication, and testing of genetic purity in soybean varieties.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권1호
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• pp.29-34
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• 2022

Silkworms have recently attracted attention as healthy functional foods. Different varieties of silkworms have functional differences; thus, there is an emerging need for variety identification. In this study, we sequenced complete mitochondrial genomes (mitogenomes) of ten government-recommended silkworm varieties (BaekHwang, BaekOk, DaeBaek, DaeBak, DaeHwang, GoldenSilk, HanSaeng, JooHwang, KumKang, and KumOk). Comparison of these sequences allowed us to select the single nucleotide polymorphisms (SNPs) in 34 sites that are specific to six silkworm varieties: 13 in DaeBak, 8 in GoldenSilk, 9 in KumKang, 2 in BaekHwang, 1 in BaekOk, and 1 in DaeHwang. Among these each one SNP per variety was amplified by preparing variety-specific primers and then using tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR). As a result, it was possible to identify these six varieties among the ten silkworm varieties, evidencing that SNPs developed from mitogenomes are useful marker for the discrimination of genetically closer silkworm varieties.

• Proceedings of the KSPS conference
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• 대한음성학회 1996년도 10월 학술대회지
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• pp.521-528
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• 1996

This presentation explores on the perceptual characteristics of the lateral sound /l/ in CV syllables. At initial position we found that /l/ has well marked formant transitions. Then several questions arise: 1) are these formant structures dependent on the following vowel\ulcorner. 2) Are the formant transitions giving an additional cue for the identification\ulcorner Considering that the French vocalic system presents a greater variety of vowels than Spanish, several experiments were designed to verify to what extent a more extensive range of vocalic timbres contribute to the perception of /l/. Natural emissions of /l/ produced in Argentine Spanish and Canadian French CV syllables were recorded, where V was successively /i, e, a, o, u/ for Spanish and /i, e, $\varepsilon$, a, $\alpha$, o, u, y, \phi$/ for French. For each item, the segment C was maintained and V was replaced by cutting & splicing by each of the remaining vowels without transitions. Results of the identification tests for Spanish show that natural /l/ segments with low Fl and high formants F3, F4 can be clearly identified in the /i, e, u/ vowel contexts without transitions. For French subjects the combination of /l/ with a vowel without transitions reflected correct identifications for its own original vowel context in /e, $\varepsilon$, y, $\phi$/. For both languages, in all these combinations, F1 values remained rather steady along the syllable. In the case of /o, u/ very likely the F2 difference lead to a variety of perceptions of the original /l/. For example in Ilul, French subjects reported some identifications of /l/ as a vowel, mainly /y/. Our observations reinforce the importance of F1 as a relevant cue for /l/, and the incidence of the relative distance between formants frequencies of both components.

• Applied Biological Chemistry
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• 제41권2호
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• pp.160-165
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• 1998

Current discriminating technique of rice variety is known to be not objective till this time because of depending on naked eye of well trained inspector. DNA finger print method based on genetic character of rice has been indicated inappropriate for on-site application, because the method need much labor and skilled expert. The purpose of this study was to develops the identification technique of polished rice varieties using CCD camera images. To minimize the noise of the captured image, thresholding and median filtering were carried out, and edge was extracted from the image data. Image data after pretreatment of normalize and FFT(fast fourier transform) were used for library model and feedforward backpropagation neural network model. Image processing technique using CCD camera could discriminate the variety of rice with high accuracy in case of quite different rice of shape, but the accuracy was reached at 85% in the similar shape of rice.

• The Journal of the Korea Contents Association
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• 제15권12호
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• pp.421-435
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• 2015

The purpose of this study was to explore the relationship between authentic leadership, organizational identification, job performance, and turnover intention. Specifically, the study investigated whether subordinates' organizational identification mediates the relationship between authentic leadership and subordinates' job performance and turnover intention. The study tested the mediated model using data from 299 employees working in a variety of organizations. The result revealed that authentic leadership was positively related to organizational identification and job performance but was negatively related to turnover intention. Further, organizational identification partially mediated the effects of authentic leadership on job performance and turnover intention. Theoretical and practical implications and limitations of the study were discussed.

• Journal of the Korean Society for Precision Engineering
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• 제18권7호
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• pp.72-84
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• 2001

This paper proposes a design methodology for customer, assembly and disassembly considering recycling. The disign process starts with the identification of customer needs, which are in turn converted into functional requirements. The concepts of Design for Customer(DFC), Design for Assembly(DFA), Design for Variety(DFV) and Design for Disassembly(DFD) are considered in the product design phases in order to decreas production variety and mass customization. And, a new module generation approach is developed for rearranging and clustering parts and subassemblies for disassembly and recycling. Based on the result of the module generation, a new configuration methodology is suggested to minimize the disassembly time or number of disassembly operations for recycling.

• Journal of Microbiology
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• 제44권2호
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• pp.137-144
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• 2006

The problems associated with gene identification and the prediction of gene structure in DNA sequences have been the focus of increased attention over the past few years with the recent acquisition by large-scale sequencing projects of an immense amount of genome data. A variety of prediction programs have been developed in order to address these problems. This paper presents a review of the computational approaches and gene-finders used commonly for gene prediction in eukaryotic genomes. Two approaches, in general, have been adopted for this purpose: similarity-based and ab initio techniques. The information gleaned from these methods is then combined via a variety of algorithms, including Dynamic Programming (DP) or the Hidden Markov Model (HMM), and then used for gene prediction from the genomic sequences.

• Journal of Information Processing Systems
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• 제7권4호
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• pp.561-580
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• 2011

This paper describes different aspects of a typical RFID implementation. Section 1 provides a brief overview of the concept of Automatic Identification and compares the use of different technologies while Section 2 describes the basic components of a typical RFID system. Section 3 and Section 4 deal with the detailed specifications of RFID transponders and RFID interrogators respectively. Section 5 highlights different RFID standards and protocols and Section 6 enumerates the wide variety of applications where RFID systems are known to have made a positive improvement. Section 7 deals with privacy issues concerning the use of RFIDs and Section 8 describes common RFID system vulnerabilities. Section 9 covers a variety of RFID security issues, followed by a detailed listing of countermeasures and precautions in Section 10.

• Journal of the Korea Furniture Society
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• 제26권3호
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• pp.274-285
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• 2015

Wood carving has been extremely widely practiced, but survives much less well than the other main materials, being vulnerable to decay, insect damage, and fire. It therefore forms an important hidden element in the art history of many cultures. Even though wood is less durable than either steel or stone, it has been used for a long time due to its usefulness. Wood has a lot of benefits. So people have used wood for materials in houses, trains, cars, bridges, and simple utilities in their ways according to their own religions, climates, and environment they are living in. Nowadays, there are wood products that are made up for its weaknesses and this has made wood be used in variety fields. Moreover, wood has been widely selected materials for sculptures, interior, and also for architecture thanks to its colors and textures. Wood has helped our life more abundant and beautiful.

• Journal of Genetic Medicine
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• 제10권1호
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• pp.27-32
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• 2013

Post-transcriptional nucleotide sequence modification of transcripts by RNA editing is an important molecular mechanism in the regulation of protein function and is associated with a variety of human disease phenotypes. Identification of RNA editing sites is the basic step for studying RNA editing. Databases and bioinformatics resources are used to annotate and evaluate as well as identify RNA editing sites. No method is free of limitations. Correctly establishing an analytic pipeline and strategic application of both experimental and bioinformatics methods constitute the first step in investigating RNA editing. This review summarizes modern bioinformatics approaches and related resources for RNA editing research.