• Journal of Pharmaceutical Investigation
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• 제30권4호
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• pp.253-258
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• 2000

In order to design the oral vaccine delivery system, we prepared the alginate micro spheres containing GFP (green fluorescent protein) as a model drug by spray method. To optimize the preparation conditions of microspheres, we investigated the effects of various parameters including nozzle pressure, nozzle opening angle, and concentrations of sodium alginate and calcium chloride. The prepared microspheres were evaluated by measuring their sizes, loading efficiency, and morphology. The particle size of microspheres was affected by the concentration of sodium alginate and calcium chloride, nozzle pressure, and nozzle opening angle. As the concentration of sodium alginate increased, GFP loading efficiency and particles size of microsphere also increased. However, it was observed to be difficult to spray the sodium alginate solution with concentration greater than 1.5% (w/v), due to high viscosity. The pressure over $3\;kgf/cm^2$ didn't affect the size of particles. As a result, the spraying method enabled us to prepare microspheres for oral vaccine delivery system. In this study, microspheres prepared with 1% (w/v) sodium alginate had greater loading efficiency and better spherical shape.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2003년도 2003 Annual Meeting, BioExhibition and International Symposium
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• pp.55-62
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• 2003

The mucosal immune system provides a first line of defense against invasion of infectious agents via inhalation, ingestion and sexual contact. For the induction of protective immunity at these invasion sites, one must consider the use of the CMIS, which interconnects inductive tissues, including PP and NALT, and effector tissues of the intestinal, respiratory and genitourinary tracts. In order for the CMIS to induce maximal protective mucosal immunity, co-administration of mucosal adjuvant or use of mucosal antigen delivery vehicle has been shown to be essential. When vaccine antigen is administered via oral or nasal route, antigen-specific Th 1 and Th2 cells, cytotoxic T lymphocytes(CTLs) and IgA B cell responses are effectively induced by the CMIS. In the early stages of induction of mucosal immune response, the uptake of orally or nasally administered antigens is achieved through a unique set of antigen-sampling cells, M cells located in follicle-associated epithelium(FAE) of inductive sites. After successful uptake, the antigens are immediately processed and presented by the underlying DCs for the generation of antigen-specific T cells and IgA committed B cells. These antigen-specific lymphocytes are then home to the distant mucosal effector tissues for the induction of antigen-specific humoral(e.g., IgA) and cell-mediated (e.g., CTL and Th1) immune responses in order to form the first line of defense. Elucidation of the molecular/cellular characteristics of the immunological sequence of mucosal immune response beginning from the antigen sampling and processing/presentation by M cells and mucosal DCs followed by the effector phase with antigen-specific lymphocytes will greatly facilitate the design of a new generation of effective mucosal antigen-specific lymphocytes will greatly facilitate the design of a new generation of a new generation of effective mucosal adjuvants and of a vaccine deliver vehicle that maximizes the use of the CMIS.

• Clinical and Experimental Vaccine Research
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• 제12권3호
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• pp.240-248
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• 2023

Purpose: We reported a survey-based study assessing the parental intention to vaccinate children of 5 to 7 years old against coronavirus disease 2019 (COVID-19). The aim of this study is to assess factors influencing the parental intention to vaccinate their children against COVID-19. Materials and Methods: This study adopted a cross-sectional design, held at the public health center of Senen district, Jakarta, Indonesia from November 1-30, 2022. The off-line questionnaires were distributed via the school administrator to all eligible parents. Factors associated with intention to vaccinate were analyzed with the regression logistic models. Results: Of the 435 parents in this study, 215 had already vaccinated their children against COVID-19 (49.4%), and the overall intention of the participants to vaccinate was 69.7%. Factors associated with intention to vaccinate the children against COVID-19 were parental employment status, parental COVID-19 vaccine status and concern of contracting COVID-19. Parents who are employed, had completed vaccines with COVID-19 booster vaccine, and had concern of their children contracting COVID-19 were more likely to vaccinate their children (odds ratio [OR], 2.10; 95% confidence interval [CI], 1.22-3.69; p=0.011; OR, 2.15; 95% CI, 1.21-3.83; p=0.013; OR, 2.40; 95% CI, 1.34-4.30; p=0.004, respectively). Concern on the vaccine's side effects was negatively associated with the willingness to vaccinate. Conclusion: This study showed that childhood COVID-19 vaccine only covered half of the population, with parental intentions for childhood COVID-19 vaccination being high, reaching almost two-thirds of the study participants. Factors influencing parental intentions were employment status, parental COVID-19 vaccine status, concerns about COVID-19 and concerns about vaccine side effects.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2000년도 제52차 추계학술대회 연제집
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• pp.112-113
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• 2000

• 한국동물위생학회지
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• 제21권3호
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• pp.277-284
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• 1998

Serum samples were collected from 100 breeders and their progeny 600 broilers. The breeders and broilers were vaccinated against infectious bronchitis(IB), infectious bursal disease(IBD) and Newcastle disease(ND) viruses according to general vaccination program. The antibodies in serum samples against IB, IBD and ND viruses were detected by ELISA using commercial ELISA kit. Geometric mean titer(GMT) of ELISA was monitored from 1-day-old to 35-day-old broilers and compared to that of breeder chickens. The GMT of ELISA to IB, IBD and ND was declined half level of the day old broiler's antibody titers at about 4, 9 and 4 days of age. The GMT of ELISA to IB, IBD and ND was declined than that of protective antibody titer at about 12, 11, and 15 days of age. Thereafter, the GMT of ELISA to IB, ND were declined and disappeared according to age of broilers. The GMT of ELISA to IBD was declined according to age of broilers, but at 25 days of age increased and 31 days of age increased than that of protective antibody titer. Taken together, these studies led to conclusion that time-course of antibody titers of broilers from vaccinated breeders and that of progeny broliers which vaccinated according to vaccine program. Those are very important data to design vaccine program to breeders and broilers.

• 융합보안논문지
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• 제8권4호
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• pp.191-197
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• 2008

악성 소프트웨어로 인한 피해가 나날이 급증하면서, 컴퓨터 사용자가 보안상 안전하게 사용할 수 있는 환경이 필수적으로 요구되고 있다. 일반적인 백신 프로그램은 악성코드가 실행된 이후에 이를 탐지한다. 이러한 백신 프로그램은 알려진 악성코드에 대해서는 효율적인 결과를 보이지만, 실행 전 응용 소프트웨어에 포함되어 있는 악성코드의 검출에 대해서는 그 기능이 없거나 부족한 실정이다. 이에 본 논문에서는 응용 소프트웨어의 실행 전 악성코드의 유무를 판단하기 위해 응용소프트웨어의 안전성 검증 시스템을 제안한다. 제안하는 안전성 검증 시스템은 악성코드의 흐름 유형을 파악하여 소프트웨어가 실행되기 전 이를 탐지함으로서 악성코드로 인해 일어날 수 있는 피해를 줄일 수 있는 계기가 될 것으로 사료된다.

• 컴퓨터교육학회논문지
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• 제18권6호
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• pp.33-42
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• 2015

본 연구는 해석수준이론을 바탕으로 정보보안 백신 소프트웨어를 필요로 하는 시점과 정보보안 백신의 광고메시지 유형, 정보보안 지식수준에 따라 사용자의 정보보안 백신 소프트웨어 채택 의도가 어떠한 차이를 나타내는지 알아보았다. 이를 위해 정보보안 백신 제품을 대상으로 2(지식수준: 고/저) ${\times}2$(시간적 거리: 가까운 거리/먼 거리) ${\times}2$(광고메시지 유형: How(구체적)/Why(추상적)) 실험을 설계한 후, 실험을 실시하였다. 분석 결과 시간적 거리와 광고메시지 유형에 따라 정보보안 백신 채택 의도에 차이가 있음을 확인하였고, 정보보안 지식수준에 따라서도 선택이 달라짐을 확인하였다. 이런 결과는 사용자의 정보보안 행동을 높이기 위해서는 사용자의 지식수준과 정보보안 시점에 따라 백신 소프트웨어에 대한 소개를 달리하는 전략을 수립해야 함을 시사한다. 특히 지식수준이 높은 사용자에게는 시기적으로 적합한 설득메시지 고려가 중요하고, 지식수준이 낮은 사용자의 올바른 백신 소프트웨어 채택을 위해서 시간적 거리에 따른 추상적 사고력을 계발할 필요성이 있음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.813-819
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• 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) induces severe respiratory impairment with a reported mortality rate of ~36% in humans. The absence of clinically available MERS-CoV vaccines and treatments to date has resulted in uncontrolled incidence and propagation of the virus. In vaccine design, fusion with the IgG Fc domain is reported to increase the immunogenicity of various vaccine antigens. However, limited reports have documented the potential negative effects of Fc fusion on vaccine antigens. To determine whether Fc fusion affects the immunogenicity of MERS-CoV antigen, we constructed a Fcassociated MERS-CoV spike protein (eS770-Fc, 110 kDa), whereby human IgG4 Fc domain was fused to MERS-CoV spike protein (eS770) via a Gly/Pro linker using baculovirus as the expression system. For comparative analyses, two eS770 proteins lacking the IgG4 Fc domain were generated using the IdeS protease ($eS770-{\Delta}Fc$) or His tag attachment (eS770-His) and the immunogenicity of the above constructs were examined following intramuscular immunization in mice. Contrary to expectations, non-Fc spike proteins ($eS770-{\Delta}Fc$, eS770-His; 90 kDa) showed higher immunogenicity than the Fc fusion protein (eS770-Fc). Moreover, unlike non-Fc spike proteins, eS770-Fc immunization did not elicit neutralizing antibodies against MERS-CoV. The lower immunogenicity of Fc-fused eS770 was related to alterations in the structural conformation of the spike protein. Taken together, our results indicate that IgG Fc fusion reduces the immunogenicity of eS770 by interfering with the proper folding structure.

• Journal of Veterinary Science
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• 제19권6호
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• pp.817-826
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• 2018

The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.

• 한국컴퓨터정보학회논문지
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• 제10권6호
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• pp.127-136
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• 2005

최근 악성코드에 의한 피해는 상업용 백신의 지속적인 개발에도 불구하고 급격히 증가되고 있다. 일반적으로 백신은 이미 알려진 악성코드는 효과적으로 탐색이 가능하지만 아무런 정보가 없는 악성코드를 탐색하기는 어려우며, 또한 최근의 악성코드들은 백신의 갱신속도보다 훨씬 빨리 새로운 변종들을 만들어내고 있기 때문에 백신의 대응이 늦게 되는 경향이 있다. 본 논문에서는 이러한 악성코드들을 효과적으로 탐색할 수 있는 탐색도구의 설계 및 개발에 관하여 기술한다 본 연구의 도구는 악성코드의 기능을 분석하여 특정한 시그너처를 추출함으로서 기존의 악성코드들 뿐 아니라 새로운 악성코드와 그 변종들에 대해서도 능동적으로 대처할 수 있다.