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Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities

  • Fu, Bo;Ren, Liang;Liu, Di;Ma, Jian-Zhang;An, Tie-Zhu;Yang, Xiu-Qin;Ma, Hong;Zhang, Dong-Jie;Guo, Zhen-Hua;Guo, Yun-Yun;Zhu, Meng;Bai, Jing
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.12
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    • pp.1703-1712
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    • 2015
  • The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB- oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.

Sedimentary facies of the Cambrian Sesong Formation, Taebacksan Basin (태백산분지 캠브리아기 세송층의 퇴적상)

  • Joo, Hyun;Ryu, In-Chang
    • Economic and Environmental Geology
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    • v.45 no.5
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    • pp.565-578
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    • 2012
  • Sedimentary facies of the Middle to Upper Cambrian Sesong Formation, Taebacksan Basin, are analyzed using detailed field mapping and stratigraphic section measuring. As a result, five sedimentary facies are recognized in the formation, which include lime nodule bearing shale facies, anastomosing wackestone-packstone facies, well-laminated siltstone facies, fine to medium sandstone facies and lime pebble conglomerate facies. Together with sedimentary facies analysis, study on vertical facies variation indicates that the Sesong Formation was deposited in an outer to inner shelf during relative sea-level fall. Especially, shallow marine aspects of the upper part of the Sesong Formation including 10-m-thick, fine to medium-grained sandstones appear to be very similar with the shallow marine strata accumulated during the Steptoean Stage (Dunderbergia) in Laurentia. These lithofacies comparisons of coeval strata between two continents suggest that sedimentation in the Sesong Formation reflects the influence of global sea-level fall occurred during the late Middle Cambrian to early Late Cambrian. As well, a stratigraphic discontinuity surface that may have sequence stratigraphic significance is recognized within the shallow marine sandstone beds of the uppermost Sesong Formation. This stratigraphic discontinuity surface may correspond to the Sauk II-III sequence boundary in Laurentia. Therefore, results delineated in this study will use a new stratigraphic paradigm for regional correlation of the Middle to Late Cambrian strata (e.g., the Sesong Formation) in the Taebacksan Basin, and will provide very useful information on intercontinental stratigraphic correlation in the future.

Implementation of Rule Management System for Validating Spatial Object Integrity (공간 객체 무결성 검증을 위한 규칙 관리 시스템의 구현)

  • Go, Goeng-Uk;Yu, Sang-Bong;Kim, Gi-Chang;Cha, Sang-Gyun
    • Journal of KIISE:Software and Applications
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    • v.26 no.12
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    • pp.1393-1403
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    • 1999
  • 공간 데이타베이스 시스템을 통하여 공유되는 공간 데이타는 무결성이 적절하게 유지되지 않는 한 전체 응용 시스템의 행위를 예측할 수 없게 되므로 데이타의 무결성 확인 및 유지는 필수적이다. 특히 공공 GIS에 저장된 공간 데이타는 토지 이용도 평가, 도시 계획, 자원 관리, 시설물 관리, 안전 관리, 국방 등 국가 전체 및 지역의 중요한 정책 결정을 위한 다양한 응용 시스템들에 의해 이용되므로 적절한 공간 객체의 무결성 확인이 더욱 더 필요하다. 본 논문에서는 능동(active) DBMS의 능동 규칙(active rule) 기법을 이용하여 공간 객체의 무결성 확인을 지원하기 위한 규칙 관리 시스템을 제시한다. 능동 규칙을 이용한 공간 객체의 무결성 확인은 응용 프로그래머를 무결성 확인에 대한 부담으로부터 자유롭게 할 수 있다. 본 시스템은 특정 DBMS에 종속되지 않는 독립적인 외부 시스템으로 존재하며, 능동 규칙 관리기, 규칙 베이스, 그리고 활성규칙 생성기의 3 부분으로 구성된다. 사용자가 공간 데이타베이스 응용 프로그램을 통해 공간 객체를 조작하고자 할 때, 본 시스템은 데이타베이스 트랜잭션을 단위로 조작되는 모든 공간 객체의 무결성 확인을 위해 응용 프로그램에 삽입될 무결성 제약조건 규칙들을 효율적으로 관리하는 역할을 한다.Abstract It is necessary that the integrity of spatial data shared through the spatial database system is validated and appropriately maintained, otherwise the activity of whole application system is unpredictable. Specially, the integrity of spatial data stored in public GIS has to be validated, because those data are used by various applications which make a decision on an important policy of the region and/or whole nation such as evaluation of land use, city planning, resource management, facility management, risk management/safety supervision, national defense. In this paper, we propose rule management system to support validating the integrity of spatial object, using the technique of active rule technique from active DBMS. Validating data integrity using active rules allows database application programmer to be free from a burden on validation of the data integrity. This system is an independent, external system that is not subject to specific DBMS and consists of three parts, which are the active rule manager, the rule base, and the triggered rule generator. When an user tries to manipulate spatial objects through a spatial database application program, this system serves to efficiently manage integrity rules to be inserted into the application program to validate the integrity constraints of all the spatial objects manipulated by database transactions.

Clinical Characteristics of Acute Drug Intoxication in Emergency Department

  • Kim In Byung;Chun Sung Pil;Kim Seung Whan
    • Biomedical Science Letters
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    • v.10 no.3
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    • pp.299-303
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    • 2004
  • The number of acute drug intoxication who visiting to emergency department which is located near urban and rural area concomitantly is inclined to increase slightly, The purpose of this study was the investigation of methods and strategies of management of acute drug intoxication in emergency department Clinical trials were done on 92 cases of acute drug intoxication visiting to Emergency Department of Chungnam National University Hospital during 4 months from April to July 2003. 1. The total number of acute drug intoxication during this period was 92 patients, which presented 0.95% of all the emergency department visiting patients during the study. The intentional ingestions were observed in 84 cases (91.3%). 2. The maximal point of age distribution was 4th decade. The number of that was 21 cases (22.8%). 3. The number of patients who had ingested benzodiazepines and doxylamine succinates was 26 cases (28.2%), organophosphorus was 20 (21.7%), paraquat was 10 (10.8%), others were 36 (39.3%). 4. Five kinds of po1ydrug ingestion was observed in 3 cases, four kinds was observed in 3, three kinds was observed in 5 and co-ingestion of alcohol was observed in 28 cases (30.4%). 5. The mortality was occurred in 4 cases, 2 of them were caused by paraquat, 1 of them was caused by organophosphorus (OP), 1 of them was caused by acetic acid. 6. The gastric larvage as a mean of treatment modality was done in 57 cases (61.9%). The use of activated charcoal was done in 8 (8.69%). The maintenance of tracheal intubation was done in 6 (6.52%). 7. The mean observational period in emergency department was 8 hours 42 minutes in benzodiazepine and doxylamine succinate group, 21 hours 46 minutes in OP, 20 hours 39 minutes in other germicidal except OP, 23 hours 9 minutes in paraquat group. Without a drug information and intoxication center in Korea, We should minimize the exhaustion of medical resources by establishment of determinant criteria which can be seen in relatively less-complicated cases of acute drug intoxication. Thus, we should consider the introduction of simple toxicology treatment protocol and toxicologic observation unit in emergency department as possible means to reduce economical and social burdens.

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Comparison of the Estimated Result of Ecosystem Service Value Using Pixel-based and Object-based Analysis (화소 및 객체기반 분석기법을 활용한 생태계서비스 가치 추정 결과 비교)

  • Moon, Jiyoon;Kim, Youn-soo
    • Korean Journal of Remote Sensing
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    • v.33 no.6_3
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    • pp.1187-1196
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    • 2017
  • Despite the continuing effort to estimate the value of function and services of ecosystem, most of the researches has used low and medium resolution satellite imagery such as MODIS or Landsat. It means that the researches to measure the ecosystem service value using VHR (Very High Resolution) satellite imagery have not been performed much, while the source of available VHR imagery is increasing. Thus, the aim of this study is to estimate and compare the result of ecosystem service value over Sejong city, S. Korea, which is one of the rapidly changed city, through the pixel-based and object-based classification analysis using VHR KOMPSAT-3 images, for more specific and precise information. In the result of the classification, forest and grassland were underestimated while agriculture and urban were overestimated in the pixel-based result compared to the object-based result. Furthermore, bare soil area was presented contrasting result that was increased in the pixel-based result, however, decreased in the object-based result. Using those results, ecosystem service values were estimated. The annual ecosystem service values in 2014 were $8.18 million USD(pixel-based) and $8.63 million USD(object-based), however, decreased to $7.80 million USD(pixel-based) and $8.62 million USD(object-based) in 2016. It is expected to use those results as a preliminary data when to make sustainable development plan and policy to improve the quality of life in the local level.

Multi-step Ahead Link Travel Time Prediction using Data Fusion (데이터융합기술을 활용한 다주기 통행시간예측에 관한 연구)

  • Lee, Young-Ihn;Kim, Sung-Hyun;Yoon, Ji-Hyeon
    • Journal of Korean Society of Transportation
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    • v.23 no.4 s.82
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    • pp.71-79
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    • 2005
  • Existing arterial link travel time estimation methods relying on either aggregate point-based or individual section-based traffic data have their inherent limitations. This paper demonstrates the utility of data fusion for improving arterial link travel time estimation. If the data describe traffic conditions, an operator wants to know whether the situations are going better or worse. In addition, some traffic information providing strategies require predictions of what would be the values of traffic variables during the next time period. In such situations, it is necessary to use a prediction algorithm in order to extract the average trends in traffic data or make short-term predictions of the control variables. In this research. a multi-step ahead prediction algorithm using Data fusion was developed to predict a link travel time. The algorithm performance were tested in terms of performance measures such as MAE (Mean Absolute Error), MARE(mean absolute relative error), RMSE (Root Mean Square Error), EC(equality coefficient). The performance of the proposed algorithm was superior to the current one-step ahead prediction algorithm.

Development of an Verification System for Enhancing BIM Design Base on Usability (활용성을 고려한 BIM 설계 오류 검증시스템 개발)

  • Yang, Dong-Suk
    • Land and Housing Review
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    • v.8 no.1
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    • pp.23-29
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    • 2017
  • The BIM design is expected to expand to the domestic and overseas construction industries, depending on the effect of construction productivity and quality improvement. However, with the obligation of Public Procurement Service to design the BIM design, it includes a design error and the problem of utilization of 3D design by choosing a simple 2D to 3D remodelling method that can not be modelled in 3D modeling or use of the construction and maintenance phases. The results reviewed by BIM design results were largely underutilized and were not even performed with the verification of the error. In order to resolve this, one must develop the check system that secures the quality of BIM design and ensure that the reliability of BIM results are available. In this study, it is designed to develop a program that can automatically verify the design of the BIM design results such as violation of the rules of the BIM design, design flaws, and improve the usability of the BIM design. In particular, this programs were developed not only to identify programmes that were not commercially available, but also to validate drawings in low-light computer environments. The developed program(LH-BIM) store the information of attribute extracted from the Revit file(ArchiCAD, IFC file included) in the integrated DB. This provides the ability to freely lookup the features and properties of drawings delivered exclusively by the LH-BIM Program without using the Revit tools. By doing so, it was possible to resolve the difficulties of using traditional commercial programs and to ensure that they operate only with traditional PC performance. Further, the results of the various BIM software can be readily validated, which can be solved the conversion process error of IFC in the case of SMC. Additionally, the developed program has the ability to automatically check the error and design criteria of the drawings, as well as the ability to calculate the area estimation. These functions allow businesses to apply simple and easy tasks to operate tasks of BIM modelling. The developed system(LH-BIM) carried out a verification test by reviewing the review of the BIM Design model of the Korea Land & Housing Corporation. It is hoped that the verification system will not only be able to achieve the Quality of BIM design, but also contribute to the expansion of BIM and future construction BIM.

Antioxidant Activities of Ethanol and Water Extracts from Propolis (프로폴리스 에탄올 및 물 추출물의 항산화 활성)

  • Jeong, Chang-Ho;Shin, Chang-Sik;Bae, Young-Il;Shim, Ki-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.12
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    • pp.1725-1730
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    • 2010
  • To obtain basic information on the potential use of propolis as a raw material in functional food, proximate composition, total phenolics content and antioxidant activities of different propolis extracts in Korea were investigated. Propolis had the highest level of crude fat and the lowest level of crude fiber. The total phenolics content of ethanol and water extract of propolis from Geochang (GEE and GWE), ethanol and water extract of propolis from Jeju (JEE and JWE) were 184.17, 316.19, 204.33 and 47.83 mg gallic acid equivalent/g, respectively. GWE contained relatively higher levels of total phenolics than the other extracts. The antioxidant potential of the extracts was assessed by different in vitro assays such as DPPH, ABTS, reducing power, ferric reducing/antioxidant power (FRAP) and peroxidation inhibiting activities through linoleic acid emulsion system. DPPH and ABTS radical scavenging activities of all the extracts were dose dependent. The GWE exhibited the best performance in reducing power, FRAP, and lipid peroxidation using ferric thiocyanate (FTC) assay. These results demonstrated that GWE has excellent antioxidant activities and thus it has great potential as a raw material for functional food.

Effect of the Combined Using of Fly Ash and Blast Furnace Slag as Cementitious Materials on Properties of Alkali-Activated Mortar (결합재(結合材)로 플라이애시와 고로(高爐)슬래그의 혼합사용(混合使用)이 알칼리 활성(活性) 모르타르의 특성(特性)에 미치는 영향(影響))

  • Koh, Kyung-Taek;Kang, Su-Tae;Park, Jung-Jun;Ryu, Gum-Sung;Lee, Jang-Hwa;Kang, Hyun-Jin
    • Resources Recycling
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    • v.19 no.4
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    • pp.19-28
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    • 2010
  • Attempts to increase the utilization of a by-products such as fly ash and blast furnace slag to partially replace the cement in concrete are gathering momentum. But most of by-products is currently dumped in landfills, thus creating a threat to the environment. Many researches on alkali-activated concrete that does not need the presence of cement as a binder have been carried out recently. However, most study deal only with alkali-activated blast furnace slag or fly ash, as for the combined use of the both, little information is reported. In this study, we investigated the influence of mixture ratio of fly ash/slag, type of alkaline activator and curing condition on the workability and compressive strength of mortar in oder to develop cementless alkali-activated concrete. In view of the results, we found out that the mixture ratio of fly ash/slag and the type of alkaline activator always results to be significant factors. But the influence of curing temperature in the strength development of mortar is lower than the contribution due to other factors. At the age of 28days, the mixture 50% fly ash and 50% slag activated with 1:1 the mass ratio of 9M NaOH and sodium silicate, develop compressive strength of about 65 MPa under $20^{\circ}C$ curing.

Semiology as a Way of Expression for Message-focused on the advertising design- (메시지 표현방법으로서의 기호-광고 디자인을 중심으로-)

  • 박영희
    • Archives of design research
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    • no.18
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    • pp.113-121
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    • 1996
  • Persuasion is one of the attractive channels to enhance the relation between markerters and consumers and deliver the true communication on products to the consumers. This arctic tried to examine the symbolic meaning system as a way of maximizing the visual communicational effect of persuasion, the efforts of marketers who are trying to utilize the psychology of the mass that consume the symbol rather than products and the symbol system as a mean of propaganda were analyzed as well. People in modern age, in general, place more value on the emotional assoessibility than the efficiency of the product, As a result, the ways of expression of propaganda approaching the mass are in the process of gradual change, which was another theme this article tried to explore. Ames sied that the human preoeption has a tendency to perceive things in some organized pattern, which can be applied to even untransparable and meaningless image. Human beings don't perceive what there is but what, thery believe there to be, and his peroeption are channelized by the opportunity of the past, his peroeption are channelized by the opportunity of the past, his experience of the environment, and the history of learning. To say another word, people not only recognize the objective form but also accept the inside meaning of the visual object. Their reponse to the visual object, therefore, include personal cognition, judgment, and attitued. The communication in visual design reveals the culture, society, and art in a complex symbol, and make synthetic cultural interpretation possible. It's pretty attractive, effective, and reasonable method to use symbolic meaning system as a way of persuasion. It is because communication means whole process from receiving and delivering the information and message to making common meaning system, to measuring the effect of the behavior change.

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