• Toxicological Research
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• 제8권1호
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• pp.95-107
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• 1992

Basolateral and brush border membrance (BLM and BBM) vesicles of renal proximal tubules were prepared from adult male New Zealand White rabbits to develop an experimental for assessment of nephrotoxicity. PAH uptakes using BLMV, and glucose and leucine uptakes using BBMV were measured in the rabbits treated uranyl acetate. In addition, urinalysis and histopathological studies were performed to investigate the correlationship with the membrance vesicle uptakes.

• Korean Journal of Acupuncture
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• 제20권2호
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• pp.55-66
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• 2003

Objectives : The aim of this experiment is to investigate the effects of moxibustion on SP6, Sp9 and BL28 in uranyl acetate-induced acute renal failure in rats. Methods : To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later moxibustion treatment was done at SP6, Sp9 and BL28 bilaterally of the rats. And then 4 days later, we measured rats' body weight, kidney weight, serum BUN, creatinine and serum electrolytic levels(Na, K and Cl). Results : In the SP6 group, serum BUN were significantly decreased (p<0.01). In the BL28 group, serum Na level were significantly decreased (p<0.05). In the $SP6{\cdot}SP9$ group, serum creatinine level were significantly increased but serum Na level, serum Cl level were significantly decreased(p<0.05). In the $SP6{\cdot}BL28$ group, hypertrophy index of kidney and serum creatinine level were significantly decreased(p<0.01) and serum BUN were marginally significantly decreased (0.1

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 1993년도 제24회 학술대회 연제 초록
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• pp.16-17
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• 1993

• Journal of Acupuncture Research
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• 제19권6호
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• pp.49-60
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• 2002

Objective : The aim of this experiment is to investigate the effect of acupuncture and moxibustion at SP6(Sanyinjiao) and BL28(Pangguanshu) on uranyl acetate-induced acute renal failure in rats. Methods : To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later acupuncture and moxibustion treatment was done at SP6(Sanyinjiao) and BL28(Pangguanshu) bilaterally of the rats. And then 4 days later, we measured rats' body weight, kidney weight, serum BUN, creatinine and serum eletrolyte levels(Na, K and Cl). Results : In the moxibustion group, hypertrophy index of kidney and serum BUN level were decreased marginally significantly, and serum creatinine, K levels were decreased significantly. But in the acupuncture group, there was no significance at this experiment. Conclusions : SP6(Sanyinjiao) and BL28(Pangguanshu) moxibustion can decrease serum BUN, creatinine, and serum eletrolyte levels on uranyl acetate-induced acute renal failure in rats. And the study will be done continuously about non-effect in the acupuncture group at this experiment.

• 환경위생공학
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• 제12권1호
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• pp.15-23
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• 1997

The anionic exchange resins with the Dowex-1 and Amberlite CG-400 form were transformed into resin of sulfate and acetate acid form, respectively. The uranyl complex ions with SO$_{4}$$^{2-}$ and CH$_{3}$COO$^{-}$ were adsorbed on the anion exchange resion mentioned above, and these complex ions were eluted as mixture eluents of 0.7M HNO$_{3}$ - 0.5M NH$_{4}$NO$_{3}$ by anion exchange chromatography. The optimum adsorption conditions of uranyl anion complex ions adsorbed on the upper of the resin colmun were 1.5-2.0 ml/min of flow rates at pH 2.0 and adsorptive power of uranyl complex ion of sulfuric acid type were nearly consistent with the Caussion normal distribution curve, whereas the elution state of UO$_{2}$(Ac)$_{2}$$^{4-}$ with acetic acid type was departed. The weighing form obtained from resin of sulfuric acid and aceric acid type was U$_{3}$O$_{8}$ whose recovery was 91.7%. The possibility of recovering uranium from the monazite sulfate solution using a strong base anion resin, Amberlite CG-400(sulfate form), was successfully recovered more than 90%.

• 대한치과의사협회지
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• 제20권7호통권158호
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• pp.625-630
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• 1982

The author observed the behavior of inflammatory cells in the primary intension of incisional wound on the Albino rat palate. The superficial wounds (0.5mm depth) were made anteroposterior lineally by surgical knife. They were sacrified on 4, 8, 12, 16, 24, 48, 72 and 120 hr after wounding. The specimens were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer and 1% osmic acid in 0.1M cacodylate buffer, and embeddd in Epon 812. Ultrathin sections were obtained by LKB 8800, and stained with uranyl acetate/lead citrate, and observed with Corynth 500 EM.

• Nuclear Engineering and Technology
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• 제51권5호
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• pp.1322-1332
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• 2019

In this paper, we systematically investigated the influence of some selected ligands on the U-phosphate precipitation induced by soil bacteria. These organics are widely ranging from acetate, lactate, salicylate and citrate to oxalate. The results revealed that uranium could be biomineralized on bacteria as $UO_2HPO_4{\cdot}4H_2O$ or $(UO_2)_3(PO_4)_2{\cdot}4H_2O$. The influence of organic ligands on the biomineralization had clear-cut correlations with its complexation abilities to uranyl. It was clearly found that the U-phosphate biomineralization was affected noticeably by the strong ligands (oxalate and citrate). Further study discovered that when the organic ligands were uncompetitive with biotic $PO_4^{3-}$ for uranyl, the transformation of uranyl species from ${\beta}-UO_2(OH)_2$ colloidal particles to free $UO_2^{2+}$-ligands ions could facilitate the U-phosphate biomineralization. However, when the organic ligands competed with biotic $PO_4^{3-}$ for uranyl, the U-phosphate biomineralization were inhibited. Our results highlight the importance of complex interactions of strong organic ligands with uranyl during the bacterial precipitation of U-P compounds and thus for the mobilization and immobilization of radio-nuclides in the nature.

• Applied Microscopy
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• 제1권1호
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• pp.27-34
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• 1969

The compound eyes of the adult Drosophila melanogaster were. fixed in 1.25 per cent glutaraldehyde and 1 per cent osmium tetroxide buffered with sodium cacodylate at pH 7.2. Double fixed specimens were dehydrated using the alcohol series and embedded in Epon 812. They were sectioned with porter blum and JUM 5B ultra-microtome and then stained with lead hydrooxide and uranyl acetate. All thin sections were examined with Hitachi HS-7 or HU-11 electron microscope. The rhabdomere of the compound eye is composed of numerous microvilli packed, arranged, and projected from inner edge of each retinal cell. Each microvillus consisted of a centrum, about $82{\AA}$ in diameter, surrounded by the substances, about $105{\AA}$ in width, which were bounded with double membrane about $44{\AA}$ in thickness. In each inner edge of the microvilli, there was a cylinder, about $175{\AA}$ in diameter, in parallel with retinal cells, which contained a cylindrical axis about $583{\AA}$ in diameter. The surface of the outer edges .of .the microvilli was bounded with reticullar substances about $500{\AA}$ in thickness.

• Applied Microscopy
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• 제50권
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• pp.14.1-14.6
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• 2020

Scanning electron microscopy (SEM) plays a central role in analyzing structures by imaging a large area of brain tissue at nanometer scales. A vast amount of data in the large area are required to study structural changes of cellular organelles in a specific cell, such as neurons, astrocytes, oligodendrocytes, and microglia among brain tissue, at sufficient resolution. Array tomography is a useful method for large-area imaging, and the osmium-thiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods are commonly used to enhance membrane contrast. Because many samples prepared using the conventional technique without en bloc staining are considered inadequate for array tomography, we suggested an alternative technique using post-staining conventional samples and compared the advantages.

• 한국동물학회지
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• 제26권4호
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• pp.271-282
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• 1983

동면기 양서류 피부 색소세포의 미세구조를 관찰하기 위하여 무미 양서류인 개구리 (Rana nigromaculata)의 피부조직을 2.5% glutaradehyde-paraformaldehye (pH 7.2)와 2% osmium tetroxide에 전후 고정한 후 ethanol과 acetone으로 탈수, Epon 812 mixture에 포매하여 LKB ultramicrotome으로 초박절 표본을 만들어 uranyl acetate와 lead citrate로 염색하여 Jeol-100B형 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 동면기 개구리의 피부 색소세포는 대황세포, iridophore 및 흑색소 보유세포로 구성되었으며, 이들 세포의 특징은 다음과 같다. 1. 대황세포 A. 대황세포는 pterinosome과 carotinoid vesicles이 전세포질에 채워져 있었으며, 많은 ribosome과 소수의 mitochondria 및 glycogen particle이 pterinosome 사이에 분산되었다. B. Pterinosome은 크고 작은 원형 또는 타원형이며, 이 소낭속의 내부 구조물에 EK라 6가지 형 (제1형 pterinosome, 제2형 pterinosome, 제3형 pterinosome, 제4형 pterinosome, 제5형 pterinosome, 제6형 pterinosome)으로 구분되며, 특히 제1형 pterinosome, 제2형 pterinosome, 제3형 pterinosome이 발달되었따. C. Carotinoid vesicle은 작은 원형 또는 타원형이며, 대부분의 carotinoid vesicle은 핵 주변부에 덩어리 모양으로 모여있고, 일부분은 이 세포의 pterinosome 사이에 분산되어 나타났다. 2. Iridophore A. Iridophore는 볼록렌즈와 같이 나타나고 좁은 세포간 공간에 의하여, 대황세포 아래에 위치하였다. B. Iridophore는 장방형 EH는 방추형의 reflective platelet로 채워져 있었으며, 서로 평행하게 규칙적으로 배열되었다. 3. 흑색소 보유세포 A. 흑색소 보유세포는 대황세포와 iridophore와 평행하여 가장 밑부분에 위치하였다. B. 흑색소 과립은 원형 또는 타원형으로 전세포질에 채워져 있었으며, 세포 소기관은 잘 관찰되지 않았다. C. 흑색소 과립으로 채워진 흑색소 보유세포의 돌기는 iridophore의 양 측부로 \ulcorner어 올라가 대황세포와 iridophore의 사이에 위치하였다.